scholarly journals Arenibacter nanhaiticus sp. nov., isolated from marine sediment of the South China Sea

2010 ◽  
Vol 60 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Fengqin Sun ◽  
Baojiang Wang ◽  
Yaping Du ◽  
Xiupian Liu ◽  
Qiliang Lai ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
The South China Sea ◽  
Rrna Gene ◽  
Sandy Sediment ◽  
The South ◽  
China Sea ◽  
Gene Sequence Analysis

An aerobic, Gram-stain-negative, rod-shaped bacterial isolate, strain NH36AT, was isolated from a sandy sediment sample from the South China Sea. Colonies of the isolate were dark orange on M2 agar. Optimal growth was observed at pH 7.0–8.5, 30 °C and in the presence of 0.5–4.0 % (w/v) NaCl. The major fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c). The DNA G+C content was 38.9 mol%. 16S rRNA gene sequence analysis revealed that strain NH36AT was most closely related to members of the genus Arenibacter, exhibiting 94.3–96.2 % sequence similarity to the type strains of Arenibacter species. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus Arenibacter. The name Arenibacter nanhaiticus sp. nov. is proposed and the type strain is NH36AT (=LMG 24842T=CCTCC AB 208315T=MCCC 1A04137T).

scholarly journals Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter

2007 ◽  
Vol 57 (2) ◽  
pp. 219-222 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Zhi-Pei Liu ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Su-Sheng Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Emended Description

A novel marine bacterial strain, HY1T, was isolated from sediment of the South China Sea. The strain was aerobic and heterotrophic and formed saffron yellow-pigmented colonies on marine agar 2216. Cells were non-motile, Gram-negative rods, frequently occurring in chains. blastn searches revealed that the 16S rRNA gene sequence of strain HY1T showed high similarity with those of members of the genera Gillisia (91.7–93.8 %) and Salegentibacter (92.6–93.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain clustered with members of both Salegentibacter and Gillisia and phylogenetic trees constructed using three different methods (neighbour-joining, maximum-parsimony and minimum-evolution) indicated that strain HY1T clustered more frequently with members of the genus Salegentibacter. The DNA G+C content of strain HY1T was 44.4 mol% and its major cellular fatty acids (⩾5 % of the total fatty acids) were iso-15 : 1 (5.0 %), iso-15 : 0 (6.8 %), anteiso-15 : 0 (6.4 %), 15 : 0 (10.4 %), iso-16 : 0 (13.5 %), summed feature 3 (comprising iso-15 : 0 2-OH and/or 16 : 1ω7c; 6.3 %), iso-17 : 0 3-OH (5.2 %) and 17 : 0 2-OH (5.0 %). Cells contained menaquinone 6. Based on the phylogenetic and phenotypic analyses, strain HY1T should be classified as representing a novel species within the genus Salegentibacter, for which the name Salegentibacter catena sp. nov. is proposed. The type strain is HY1T (=CGMCC 1.6101T=JCM 14015T). Based on this study and on previously described Salegentibacter species, an emended description of the genus Salegentibacter is given.

Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea

2010 ◽  
Vol 60 (9) ◽  
pp. 2215-2220 ◽  
Author(s):  
Mingshuang Xu ◽  
Yuhua Xin ◽  
Yong Yu ◽  
Jianli Zhang ◽  
Yuguang Zhou ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Gene Sequence ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Bacterium Strain ◽  
Ubiquinone 10 ◽  
Sequence Similarities

A novel Gram-negative, orange-pigmented, slightly halophilic, rod-shaped bacterium, strain T30T, was isolated from sediment from the South China Sea. Phylogenetic analysis showed that strain T30T was a member of the genus Erythrobacter, sharing highest 16S rRNA gene sequence similarities with Erythrobacter aquimaris JCM 12189T (99.5 %) and Erythrobacter vulgaris DSM 17792T (99.0 %). Levels of DNA–DNA relatedness between strain T30T and closely related strains of Erythrobacter species ranged from 14.5 to 56.9 %.The isolate lacked bacteriochlorophyll a and contained ubiquinone-10 as the predominant respiratory lipoquinone. The major fatty acids of this strain were C18 : 1 ω7c (38.2 %) and C16 : 1 ω7c /C16 : 1 ω6c (17.4 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and phosphatidylglycerol. The DNA G+C content of strain T30T was 59.5 mol%. On the basis of phenotypic and phylogenetic data, a novel species, Erythrobacter nanhaisediminis sp. nov., is proposed; the type strain is T30T (=CGMCC 1.7715T=JCM 16125T).

scholarly journals Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea

2007 ◽  
Vol 57 (1) ◽  
pp. 157-160 ◽  
Author(s):  
Jun Gu ◽  
Bin Guo ◽  
Ya-Nan Wang ◽  
Su-Lin Yu ◽  
Ryuhei Inamori ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
Respiratory Quinone ◽  
China Sea ◽  
Major Respiratory Quinone ◽  
Bacterium Strain ◽  
Sequence Similarities

A Gram-negative, non-motile, rod-shaped bacterium, strain SS011B1-20T, was isolated from sediments of the South China Sea. Growth occurred at NaCl concentrations between 0 and 10 % and at temperatures between 10 and 37 °C. Strain SS011B1-20T contained Q-10 as the major respiratory quinone and C18 : 1 ω7c (81.2 %), C16 : 0 (7.0 %) and C18 : 1 methyl (4.3 %) as the predominant fatty acids. The G+C content of the genomic DNA was 64.7 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SS011B1-20T belonged to a clade within the genus Oceanicola in the Alphaproteobacteria, the highest sequence similarities being found with respect to Oceanicola batsensis (96.3 %) and with Oceanicola granulosus (94.9 %). Strain SS011B1-20T could be clearly distinguished from other Oceanicola species on the basis of the genotypic, phenotypic and phylogenetic data. Thus, it is proposed that strain SS011B1-20T represents a novel species of the genus Oceanicola, with the name Oceanicola nanhaiensis sp. nov. The type strain is SS011B1-20T (=LMG 23508T=CGMCC 1.6293T).

scholarly journals Wenxinia marina gen. nov., sp. nov., a novel member of the Roseobacter clade isolated from oilfield sediments of the South China Sea

2007 ◽  
Vol 57 (8) ◽  
pp. 1711-1716 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Su-Sheng Yang ◽  
Shuang-Jiang Liu ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
The Family ◽  
Dinoroseobacter Shibae ◽  
Novel Genus And Species ◽  
Sequence Similarities

An aerobic and heterotrophic, Gram-negative bacterial isolate, strain HY34T, was isolated from sediment of an oilfield in the South China Sea, China. The taxonomy of strain HY34T was studied by phenotypic and phylogenetic methods. Strain HY34T formed faint-pink colonies on marine agar 2216. Cells of strain HY34T were non-motile, ovoid or short rods. Strain HY34T was positive for catalase and oxidase, and nitrate was reduced to nitrite. The nearly complete 16S rRNA gene sequence of strain HY34T was obtained and sequence analysis showed that it, together with the genus Rubellimicrobium, formed a distinct clade close to some members of the Roseobacter clade in the family Rhodobacteraceae, and it showed highest sequence similarities to Oceanicola granulosus HTCC2516T (93.8 %), Silicibacter lacuscaerulensis ITI-1157T (93.3 %), Dinoroseobacter shibae DFL 12T (93.3 %) and Rubellimicrobium thermophilum C-lvk-R2A-2T (92.2 %). Bacteriochlorophyll a was not detected. The ubiquinone system was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. The major fatty acids (>10 %) were C18 : 1 ω7c and C16 : 0. The DNA G+C content of this strain was 69.4 mol%. A polyphasic analysis supported the conclusion that this strain represents a novel genus and species, which we designated Wenxinia marina gen. nov., sp. nov. The type strain of Wenxinia marina is HY34T (=CGMCC 1.6105T =JCM 14017T).

scholarly journals Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea

2007 ◽  
Vol 57 (9) ◽  
pp. 1970-1974 ◽  
Author(s):  
Bin Guo ◽  
Jun Gu ◽  
Yu-Guang Ye ◽  
Yue-Qin Tang ◽  
Kenji Kida ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Carbon Sources ◽  
The South China Sea ◽  
Rrna Gene ◽  
Organic Substrates ◽  
The South ◽  
Respiratory Quinone ◽  
Moderate Halophile ◽  
China Sea

A Gram-negative, motile, non-spore-forming and moderately halophilic ellipsoid-shaped marine coccobacillus, designated strain SS011B1-4T, was isolated from benthic sediment of the South China Sea. Optimum growth occurred at 30–37 °C, pH 7.5–8.0 and 4–8 % (w/v) NaCl. Strain SS011B1-4T utilized a variety of organic substrates as sole carbon sources, but did not utilize toluene, n-tetradecane or crude oil. Strain SS011B1-4T had ubiquinone-9 as the major respiratory quinone and C18 : 1 ω9c, C16 : 0 and C12 : 0 3-OH as the predominant fatty acids. The genomic DNA G+C content was 62.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS011B1-4T belonged to the genus Marinobacter of the Gammaproteobacteria. The results of the phenotypic, phylogenetic and genomic analyses revealed that strain SS011B1-4T represents a novel species of the genus Marinobacter. The name Marinobacter segnicrescens sp. nov. is therefore proposed, with strain SS011B1-4T (=LMG 23928T=CGMCC 1.6489T) as the type strain.

scholarly journals Cyclobacterium lianum sp. nov., a marine bacterium isolated from sediment of an oilfield in the South China Sea, and emended description of the genus Cyclobacterium

2006 ◽  
Vol 56 (12) ◽  
pp. 2927-2930 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Bao-Jun Wang ◽  
Su-Sheng Yang ◽  
Shuang-Jiang Liu
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
Phenotypic Properties ◽  
China Sea ◽  
Emended Description ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

The marine bacterial strain HY9T was isolated from sediment from the South China Sea. Strain HY9T is aerobic, heterotrophic and rose-pigmented. The cells are non-motile and curved, i.e. ring-like or horseshoe-shaped. The 16S rRNA gene sequence of strain HY9T was determined and blast searches revealed that it possessed significant sequence similarities with respect to Cyclobacterium species (92.8–93.6 %). Phylogenetic analysis confirmed that strain HY9T was tightly clustered with members of the genus Cyclobacterium. The cellular morphology and chemotaxonomic and phenotypic properties of strain HY9T showed that it should be classified as a member of the genus Cyclobacterium. Significant evolutionary distances and a range of phenotypic features distinguished strain HY9T from previously described Cyclobacterium species. Hence, strain HY9T represents a novel species in the genus Cyclobacterium, for which the name Cyclobacterium lianum sp. nov. is proposed. The type strain is HY9T (=CGMCC 1.6102T=JCM 14011T). On the basis of this study and previously described properties of Cyclobacterium species, an emended description of the genus Cyclobacterium is proposed.

scholarly journals Oceanicola nitratireducens sp. nov., a marine alphaproteobacterium isolated from the South China Sea

2010 ◽  
Vol 60 (7) ◽  
pp. 1655-1659 ◽  
Author(s):  
Qiang Zheng ◽  
Chuang Chen ◽  
Ya-Nan Wang ◽  
Nianzhi Jiao
Keyword(s):  
South China Sea ◽  
South China ◽  
Phylogenetic Analyses ◽  
The South China Sea ◽  
Rrna Gene ◽  
Beibu Gulf ◽  
16S Rrna Gene Sequences ◽  
The South ◽  
Phenotypic Data ◽  
China Sea

A Gram-negative, non-motile, short-rod-shaped bacterial strain (JLT1210T) that accumulates poly-β-hydroxybutyrate granules was isolated from the Beibu Gulf in the South China Sea. Cells have polar or subpolar flagella. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belongs to the genus Oceanicola in the order Rhodobacterales, class Alphaproteobacteria. The closest neighbours were Oceanicola nanhaiensis SS011B1-20T (96.5 % similarity) and Oceanicola batsensis HTCC2597T (96.4 %). The predominant respiratory ubiquinone of strain JLT1210T was Q-10 and the DNA G+C content was 72.8 mol%. Evidence from genotypic, chemotaxonomic and phenotypic data shows that strain JLT1210T represents a novel species of the genus Oceanicola, for which the name Oceanicola nitratireducens sp. nov. is proposed; the type strain is JLT1210T (=LMG 24663T=CGMCC 1.7292T).

scholarly journals Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea

2010 ◽  
Vol 60 (4) ◽  
pp. 985-989 ◽  
Author(s):  
Jian Wang ◽  
Yan Li ◽  
Jiang Bian ◽  
Shu-Kun Tang ◽  
Biao Ren ◽  
...  
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Ocean Sediment ◽  
Actinomycete Strain

A novel actinomycete strain, designated MS498T, was isolated from an ocean sediment sample collected from the South China Sea. It was subjected to a polyphasic analysis to determine its taxonomic position. The phylogenetic tree based on 16S rRNA gene sequences showed that strain MS498T had the highest similarity (96.5 %) with members of the genus Prauserella and was loosely associated with Prauserella rugosa DSM 43194T and Saccharomonospora halophila DSM 44411T. Based on 16S rRNA gene sequence analysis, phenotypic characteristics and chemotaxonomic data, the new isolate is proposed to represent a novel species of the genus Prauserella, named Prauserella marina sp. nov. (type strain MS498T=CCTCC AA 208056T =DSM 45268T).

scholarly journals Nocardiopsis oceani sp. nov. and Nocardiopsis nanhaiensis sp. nov., actinomycetes isolated from marine sediment of the South China Sea

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3384-3391 ◽  
Author(s):  
Hua-Qi Pan ◽  
Dao-Feng Zhang ◽  
Li Li ◽  
Zhao Jiang ◽  
Juan Cheng ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Marine Sediment ◽  
Type Strain ◽  
The South China Sea ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Aerial Hyphae

Two actinomycete strains, designated 10A08AT and 10A08BT, were isolated from marine sediment samples of the South China Sea and their taxonomic positions were determined by a polyphasic approach. The two Gram-stain-positive, aerobic strains produced branched substrate mycelium and aerial hyphae, and no diffusible pigment was produced in the media tested. At maturity, spore chains were formed on aerial hyphae and all mycelium fragmented with age. Whole-cell hydrolysates of both strains contained meso-diaminopimelic acid and no diagnostic sugars. Their predominant menaquinones (>10 %) were MK-9(H4), MK-9(H6) and MK-10(H6) for strain 10A08AT and MK-9(H4), MK-9(H6), MK-10(H4) and MK-10(H6) for strain 10A08BT. The polar lipids detected from the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and unknown phosphoglycolipids and phospholipids. The major fatty acids (>10 %) of both strains were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The genomic DNA G+C contents of strains 10A08AT and 10A08BT were 70.9 and 71.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarities, the two strains were shown to be most closely related to species of the genus Nocardiopsis. DNA–DNA hybridization relatedness values of < 70 % between these two isolates and their closest neighbour, Nocardiopsis terrae YIM 90022T, and between the two strains supported the conclusion that they represent two novel species. Based on phylogenetic analysis and phenotypic and genotypic data, it is concluded that the two isolates belong to the genus Nocardiopsis, and the names Nocardiopsis oceani sp. nov. (type strain 10A08AT = DSM 45931T = BCRC 16951T) and Nocardiopsis nanhaiensis sp. nov. (type strain 10A08BT = CGMCC 47227T = BCRC 16952T) are proposed.

A new species of the deep-sea shrimp genus Spongicoloides (Decapoda: Spongicolidae) from the South China Sea

Zootaxa ◽  
2021 ◽  
Vol 5005 (3) ◽  
pp. 276-290
Author(s):  
YU ZHAO ◽  
TING XU ◽  
WEIDI YANG ◽  
JIAN-WEN QIU
Keyword(s):  
New Species ◽  
South China Sea ◽  
South China ◽  
Deep Sea ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
The Third ◽  
The Western Pacific

Spongicoloides zhoui sp. nov. (Crustacea: Decapoda: Spongicolidae), a species of deep-sea hexactinellid sponge-associated shrimp, is described based on specimens collected from the Zhenbei Seamount in the South China Sea. The new species is morphologically most similar to the Western Pacific congeneric species Spongicoloides iheyaensis Saito, Tsuchida & Yamamoto, 2006 in that the ischium of the third pereiopod is unarmed and the fixed finger of the third pereiopod is armed with small teeth on the distoventral margin. However, S. zhoui sp. nov. can be distinguished from S. iheyaensis in that its female antennal basicerite has three large spines on the distolateral margin. Molecular analyses based on nuclear histone H3, and mitochondrial cytochrome c oxidase subunit I (COI), 12S ribosomal RNA (rRNA), and 16S rRNA gene fragments confirmed the placement of S. zhoui sp. nov. within a clade of Spongicoloides/Spongiocaris species, and their sequence divergences were large enough to justify the recognition of this new species.

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