scholarly journals Halorubrum arcis sp. nov., an extremely halophilic archaeon isolated from a saline lake on the Qinghai–Tibet Plateau, China

2007 ◽  
Vol 57 (5) ◽  
pp. 1069-1072 ◽  
Author(s):  
Xue-Wei Xu ◽  
Yue-Hong Wu ◽  
Hui-bin Zhang ◽  
Min Wu
Keyword(s):  
Salt Lake ◽  
Tibet Plateau ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Halophilic Archaeon ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Closely Related Species ◽  
Extremely Halophilic Archaeon ◽  
Qinghai Tibet Plateau

A Gram-negative, aerobic, neutrophilic and extremely halophilic archaeon (strain AJ201T), isolated from Ayakekum salt lake on the Qinghai–Tibet Plateau, was investigated by a polyphasic approach. The DNA G+C content of strain AJ201T was 65.7 mol%. The major polar lipid profile and phylogenetic analysis based on 16S rRNA gene sequences supported the allocation of the strain to the genus Halorubrum. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain AJ201T from closely related species. Therefore, strain AJ201T represents a novel species of the genus Halorubrum, for which the name Halorubrum arcis sp. nov. is proposed. The type strain is strain AJ201T (=CGMCC 1.5343T=JCM 13916T).

scholarly journals Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China

2007 ◽  
Vol 57 (7) ◽  
pp. 1619-1624 ◽  
Author(s):  
Xue-Wei Xu ◽  
Yue-Hong Wu ◽  
Zhen Zhou ◽  
Chun-Sheng Wang ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Salt Lake ◽  
Halophilic Bacteria ◽  
Fatty Acid Analysis ◽  
Tibet Plateau ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Gene Sequence Analysis ◽  
Qinghai Tibet Plateau ◽  
Type Strains

Three strains of Gram-negative, aerobic, neutrophilic and halophilic bacteria were isolated from samples of a salt lake on the Qinghai–Tibet Plateau and a subterranean saline well in the Si-Chuan Basin of China. These isolates, designated AJ275T, AJ282T and ZG16T, were investigated using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the isolates could be affiliated to the genus Halomonas. Genomic DNA G+C contents were 65.9 mol% for AJ275T, 56.7 mol% for AJ282T and 57.6 mol% for ZG16T. The results of DNA–DNA hybridizations, fatty acid analysis and physiological and biochemical tests allowed the isolates to be differentiated genotypically and phenotypically from closely related species. It is proposed that strains AJ275T (=CGMCC 1.6493T=JCM 14606T=LMG 23976T), AJ282T (=CGMCC 1.6494T=JCM 14607T=LMG 23978T) and ZG16T (=CGMCC 1.6495T=JCM 14608T=LMG 23977T) represent the type strains of three novel species in the genus Halomonas: Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., respectively.

Halopenitus malekzadehii sp. nov., an extremely halophilic archaeon isolated from a salt lake

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3232-3236 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Antonio Ventosa
Keyword(s):  
Type Species ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Brine Sample ◽  
Extremely Halophilic Archaeon

Strain CC65T, a novel extremely halophilic archaeon, was isolated from a brine sample of a salt lake in Iran. The novel strain was light yellow-pigmented, non-motile, pleomorphic and required at least 1.7 M NaCl and 0.02 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.4 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, while it was able to grow over a pH and a temperature range of pH 6.5–9.0 and 30–50 °C, respectively. Analysis of 16S rRNA gene sequence revealed that strain CC65T clustered with the sole member of the genus Halopenitus , Halopenitus persicus DC30T with a sequence similarity of 98.0 %. The polar lipid profile of strain CC65T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. An unidentified glycolipid and two minor phospholipids were also observed. The only quinone present was MK-8(II-H2). The DNA G+C content of strain CC65T was 63.8 mol%. On the basis of the biochemical and physiological characteristics, as well as DNA–DNA hybridization (44 % with Halopenitus persicus IBRC 10041T), strain CC65T is classified as a novel species of the genus Halopenitus , for which the name Halopenitus malekzadehii sp. nov. is proposed. The type strain is CC65T ( = IBRC-M 10418T = KCTC 4045T).

scholarly journals Haloterrigena hispanica sp. nov., an extremely halophilic archaeon from Fuente de Piedra, southern Spain

2007 ◽  
Vol 57 (7) ◽  
pp. 1499-1503 ◽  
Author(s):  
Ida Romano ◽  
Annarita Poli ◽  
Ilaria Finore ◽  
F. Javier Huertas ◽  
Agata Gambacorta ◽  
...  
Keyword(s):  
Lipid Analysis ◽  
Novel Species ◽  
Salt Lake ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Dna Reassociation ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon belonging to the order Halobacteriales was isolated from Fuente de Piedra salt lake, Spain. This strain, designated FP1T, was a pleomorphic coccoid, neutrophilic and required at least 15 % (w/v) NaCl for growth. Strain FP1T grew at 37–60 °C, with optimal growth at 50 °C. Mg2+ was not required, but growth was observed with up to 10 % (w/v) MgSO4. Polar lipid analysis revealed the presence of mannose-6-sulfate(1-2)-glucose glycerol diether as a major glycolipid. Both C20C20 and C20C25 core lipids were present. The genomic DNA G+C content was 62.0 mol%. Phylogenetic analysis based on comparison of 16S rRNA gene sequences demonstrated that the isolate was most closely related to species of the genus Haloterrigena. DNA–DNA reassociation values between strain FP1T and the most closely related species of the genus Haloterrigena (Haloterrigena thermotolerans, Haloterrigena saccharevitans and Haloterrigena limicola) were lower than 29 %. It is therefore considered that strain FP1T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena hispanica sp. nov. is proposed. The type strain is FP1T (=DSM 18328T=ATCC BAA-1310T).

scholarly journals Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood

2007 ◽  
Vol 57 (10) ◽  
pp. 2296-2298 ◽  
Author(s):  
Seong Woon Roh ◽  
Young-Do Nam ◽  
Ho-Won Chang ◽  
Youlboong Sung ◽  
Kyoung-Ho Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Extremely Halophilic Archaeon ◽  
Physiological And Biochemical

A novel, extremely halophilic archaeon B3T was isolated from shrimp-salted seafood. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Strain B3T is non-motile, Gram-variable, requires at least 10 % (w/v) NaCl for growth and grows in the ranges of 21–50 °C and pH 6.5–9.0. The DNA G+C content of strain B3T was 63.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain B3T belonged to the genus Halalkalicoccus and was phylogenetically closely related to the type strain Halalkalicoccus tibetensis (98.64 %). However, DNA–DNA hybridization experiments showed 7.0 % relatedness between strain B3T and a strain of a reference species of the genus Halalkalicoccus. Combined analysis of 16S rRNA gene sequences, DNA–DNA relatedness data, physiological and biochemical tests indicated that the genotypic and phenotypic characteristics differentiate strain B3T from other Halalkalicoccus species. On the basis of the evidence presented in this report, strain B3T represents a novel species of the genus Halalkalicoccus, for which the name Halalkalicoccus jeotgali. sp. nov. is proposed. The type strain is B3T (=KCTC 4019T=DSM 18796T=JCM 14584T=CECT 7217T).

scholarly journals Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria

2006 ◽  
Vol 56 (7) ◽  
pp. 1583-1588 ◽  
Author(s):  
Karima Kharroub ◽  
Teresa Quesada ◽  
Raquel Ferrer ◽  
Susana Fuentes ◽  
Margarita Aguilera ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Genomic Dna ◽  
Type Strain ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Extremely Halophilic Archaeon ◽  
Derivatives Of

A novel extremely halophilic archaeon was isolated from Ezzemoul sabkha, Algeria. The strain, designated 5.1T, was neutrophilic, motile and Gram-negative. At least 15 % (w/v) NaCl was required for growth. The isolate grew at pH 6.5–9.0, with optimum growth at pH 7.0–7.5. Mg2+ was required for growth. Polar lipids were C20C20 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, and phosphatidylglycerol sulfate and sulfated diglycosyl diether. The genomic DNA G+C content of strain 5.1T was 61.9 mol% (T m). Phylogenetic analysis based on comparison of 16S rRNA gene sequences revealed that strain 5.1T clustered with Halorubrum species. The results of DNA–DNA hybridization and biochemical tests allowed genotypic and phenotypic differentiation of strain 5.1T from other Halorubrum species. The name Halorubrum ezzemoulense sp. nov. (type strain 5.1T=CECT 7099T=DSM 17463T) is proposed.

scholarly journals Characterization ofHalorubrum sfaxensesp. nov., a New Halophilic Archaeon Isolated from the Solar Saltern of Sfax in Tunisia

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Hana Trigui ◽  
Salma Masmoudi ◽  
Céline Brochier-Armanet ◽  
Sami Maalej ◽  
Sam Dukan
Keyword(s):  
Dna Hybridization ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
The Family ◽  
Close Relationship ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain ETD6, was isolated from a marine solar saltern in Sfax, Tunisia. Analysis of the 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genusHalorubrumamong the familyHalobacteriaceae, with a close relationship toHrr. xinjiangense(99.77% of identity). However, value for DNA-DNA hybridization between strain ETD6 andHrr.xinjiangensewere about 24.5%. The G+C content of the genomic DNA was 65.1 mol% (T(m)). Strain ETD6 grew in 15–35% (w/v) NaCl. The temperature and pH ranges for growth were 20–55°C and 6–9, respectively. Optimal growth occurred at 25% NaCl, 37°C, and pH 7.4. The results of the DNA hybridization againstHrr. xinjiangenseand physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain ETD6 from otherHrr.species. Therefore, strain ETD6 represents a novel species of the genusHalorubrum, for which the nameHrr. sfaxensesp. nov. is proposed. The Genbank EMBL-EBI accession number is GU724599.

scholarly journals Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment

2010 ◽  
Vol 60 (4) ◽  
pp. 909-913 ◽  
Author(s):  
Peter Kämpfer ◽  
Jenny Schäfer ◽  
Nicole Lodders ◽  
Hans-Jürgen Busse
Keyword(s):  
Indoor Environment ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Diamino Acid ◽  
Type Strains

A Gram-stain-positive, rod-shaped, non-endospore-forming, orange-pigmented (coloured) actinobacterium (01-Je-003T) was isolated from the wall of an indoor environment primarily colonized with moulds. On the basis of 16S rRNA gene sequence similarity studies, strain 01-Je-003T was shown to belong to the genus Brevibacterium and was most similar to the type strains of Brevibacterium picturae (98.8 % similarity), Brevibacterium marinum (97.3 %) and Brevibacterium aurantiacum (97.2 %). Chemotaxonomic data [predominant quinone menaquinone MK-8(H2); polar lipid profile consisting of major compounds diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid meso-diaminopimelic acid; polyamine pattern showing major compounds putrescine and cadaverine; major fatty acids anteiso-C15 : 0 and anteiso-C17 : 0] supported the affiliation of strain 01-Je-003T to the genus Brevibacterium. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 01-Je-003T from the two most closely related species, B. picturae and B. marinum. Strain 01-Je-003T therefore represents a novel species, for which the name Brevibacterium sandarakinum sp. nov. is proposed, with the type strain 01-Je-003T (=DSM 22082T =CCM 7649T).

scholarly journals Nocardioides dongxiaopingii sp. nov., isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau

2020 ◽  
Vol 70 (5) ◽  
pp. 3234-3240 ◽  
Author(s):  
Sihui Zhang ◽  
Xiaoxia Wang ◽  
Jing Yang ◽  
Shan Lu ◽  
Xin-He Lai ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Tibet Plateau ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Qinghai Tibet Plateau

Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau, PR China. Cells were aerobic, catalase-positive and oxidase-negative. Colonies on Reasoner’s 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation. The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl. The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %). The genome of strain S-1144T showed less than 70 % digital DNA–DNA hybridization and < 95–96 % average nucleotide identity values to the above reference strains. The DNA G+C content of strain S-1144T was 73.5 mol%. MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C16 : 0, C17 : 1  ω8c, C17 : 0 and C18 : 1  ω9c. On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides , for which the name Nocardioides dongxiaopingii sp. nov. is proposed. The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).

scholarly journals Halovivax cerinus sp. nov., an extremely halophilic archaeon from a hypersaline lake

2015 ◽  
Vol 65 (Pt_1) ◽  
pp. 65-70 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Mehrnoosh Rasooli ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
...  
Keyword(s):  
Type Species ◽  
Salt Lake ◽  
Optimal Growth ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
The Novel ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain IC35T, was isolated from a mud sample of the Aran-Bidgol salt lake in Iran. The novel strain was cream, non-motile, rod-shaped and required at least 2.5 M NaCl, but not MgCl2, for growth. Optimal growth was achieved with 3.4 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 (grew over a pH range of 6.5–9.0) and 40 °C (grew over a temperature range of 30–50 °C), respectively. Analysis of 16S rRNA gene sequences revealed that strain IC35T clustered with species of the genus Halovivax , with sequence similarities of 97.3 %, 96.6 % and 96.3 %, respectively, to Halovivax limisalsi IC38T, Halovivax asiaticus EJ-46T and Halovivax ruber XH-70T. The rpoB′ gene similarities between the novel strain and Halovivax limisalsi IBRC-M 10022T, Halovivax ruber JCM 13892T and Halovivax asiaticus JCM 14624T were 90.2 %, 90.2 % and 89.9 %, respectively. The polar lipid pattern of strain IC35T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; six unknown glycolipids and two minor phospholipids were also observed. The only quinone present was MK-8 (II-H2). The G+C content of the genomic DNA was 63.2 mol%. DNA–DNA hybridization studies (29 % hybridization with Halovivax limisalsi IBRC-M 10022T), as well as biochemical and physiological characterization, allowed strain IC35T to be differentiated from other species of the genus Halovivax . A novel species, Halovivax cerinus sp. nov., is therefore proposed to accommodate this strain. The type strain is IC35T ( = IBRC-M 10256T = KCTC 4050T).

scholarly journals Natronorubrum aibiense sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China, and emended description of the genus Natronorubrum

2006 ◽  
Vol 56 (7) ◽  
pp. 1515-1517 ◽  
Author(s):  
Heng-Lin Cui ◽  
Dilbr Tohty ◽  
Jie Feng ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
Gene Sequence ◽  
Agar Medium ◽  
Novel Species ◽  
Salt Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Optimum Ph ◽  
Emended Description ◽  
Extremely Halophilic Archaeon ◽  
Sequence Similarities

An extremely halophilic archaeon (strain 7-3T) was isolated from Aibi salt lake in Xin-Jiang, China. It formed small (<1.0 mm), red, elevated colonies on salt-milk agar medium. Strain 7-3T was able to grow at pH 6.5–9.5 (optimum pH 8.0) and at 20–50 °C (optimum 45 °C). Analysis of its 16S rRNA gene sequence indicated that strain 7-3T was phylogenetically related to members of the genus Natronorubrum, with sequence similarities of 97.0 % to Natronorubrum bangense A33T and 95.2 % to Natronorubrum tibetense GA33T. The G+C content of its DNA was 61.2 mol% (T m). Levels of DNA–DNA relatedness to Nrr. bangense and Nrr. tibetense were 45 and 37 %, respectively. It is concluded that strain 7-3T (=CGMCC 1.4299T=JCM 13488T) should be classified as the type strain of a novel species of the genus Natronorubrum, for which the name Natronorubrum aibiense sp. nov. is proposed. Based on the properties of Nrr. aibiense given here and of those of Nrr. bangense and Nrr. tibetense described previously, an emended description of the genus Natronorubrum is presented.

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