scholarly journals Teredinibacter waterburyi sp. nov., a marine, cellulolytic endosymbiotic bacterium isolated from the gills of the wood-boring mollusc Bankia setacea (Bivalvia: Teredinidae) and emended description of the genus Teredinibacter

2020 ◽  
Vol 70 (4) ◽  
pp. 2388-2394 ◽  
Author(s):  
Marvin A. Altamia ◽  
J. Reuben Shipway ◽  
David Stein ◽  
Meghan A. Betcher ◽  
Jennifer M. Fung ◽  
...  
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Bacterial Species ◽  
Sole Source ◽  
Rrna Gene ◽  
Protein Coding ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Wood Boring

A cellulolytic, aerobic, gammaproteobacterium, designated strain Bs02T, was isolated from the gills of a marine wood-boring mollusc, Bankia setacea (Bivalvia: Teredinidae). The cells are Gram-stain-negative, slightly curved motile rods (2–5×0.4–0.6 µm) that bear a single polar flagellum and are capable of heterotrophic growth in a simple mineral medium supplemented with cellulose as a sole source of carbon and energy. Cellulose, carboxymethylcellulose, xylan, cellobiose and a variety of sugars also support growth. Strain Bs02T requires combined nitrogen for growth. Temperature, pH and salinity optima (range) for growth were 20 °C (range, 10–30 °C), 8.0 (pH 6.5–8.5) and 0.5 M NaCl (range, 0.0–0.8 M), respectively when grown on 0.5 % (w/v) galactose. Strain Bs02T does not require magnesium and calcium ion concentrations reflecting the proportions found in seawater. The genome size is approximately 4.03 Mbp and the DNA G+C content of the genome is 47.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences, and on conserved protein-coding sequences, show that strain Bs02T forms a well-supported clade with Teredinibacter turnerae . Average nucleotide identity and percentage of conserved proteins differentiate strain Bs02T from Teredinibacter turnerae at threshold values exceeding those proposed to distinguish bacterial species but not genera. These results indicate that strain Bs02T represents a novel species in the previously monotypic genus Teredinibacter for which the name Teredinibacter waterburyi sp. nov. is proposed. The strain has been deposited under accession numbers ATCC TSD-120T and KCTC 62963T.

Humibacter antri sp. nov., an actinobacterium isolated from a natural cave, and emended description of the genus Humibacter

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4315-4319 ◽  
Author(s):  
Soon Dong Lee
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Minor Component ◽  
Rrna Gene ◽  
Content Type ◽  
Mycolic Acids ◽  
Link Type ◽  
Hybridization Data ◽  
Emended Description

A novel high DNA G+C content bacterium, designated strain D7-27T, was isolated from clay soils collected inside a natural cave in Jeju, Republic of Korea. The cells of the organism were aerobic, Gram-stain-positive, non-motile rods; its colonies were white, circular and entire in margin. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that strain D7-27T formed a coherent cluster with Humibacter albus of the family Microbacteriaceae (97.6 % sequence similarity). The cell-wall peptidoglycan contained ornithine and 2,4-diaminobutyric acid as the diagnostic diamino acids. The major menaquinones were MK-12 and MK-11, with MK-10 as a minor component. The polar lipids consisted mainly of phosphatidylglycerol and an unknown glycolipid. Mycolic acids were not present. The predominant fatty acids were anteiso-C17 : 0 and cyclohexyl-C17 : 0. The DNA G+C content was 66.3 mol%. DNA–DNA relatedness between strain D7-27T and H. albus DSM 18994T was 28.6 % (17.6 % in a reciprocal test). On the basis of the phenotypic, chemotaxonomic and DNA–DNA hybridization data, strain D7-27T ( = KCTC 33009T = DSM 25738T) is considered as the type strain of a novel species of the genus Humibacter , for which the name Humibacter antri sp. nov. is proposed. An emended description of the genus Humibacter is also provided.

scholarly journals Skermanella stibiiresistens sp. nov., a highly antimony-resistant bacterium isolated from coal-mining soil, and emended description of the genus Skermanella

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1271-1276 ◽  
Author(s):  
Guosheng Luo ◽  
Zunji Shi ◽  
Hui Wang ◽  
Gejiao Wang
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Resistant Bacterium ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Major Respiratory Quinone

A Gram-negative, aerobic, motile, rod-shaped, antimony-resistant bacterium, designated strain SB22T, was isolated from soil of Jixi coal mine, China. The major cellular fatty acids (>5 %) were C18 : 1ω7c (63.5 %), summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I, 10.8 %) and C16 : 0 (9.9 %). The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid. The genomic DNA G+C content was 69.6 mol% and Q-10 was the major respiratory quinone. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SB22T was most closely related to Skermanella aerolata 5416T-32T (97.3 %), Skermanella parooensis ACM 2042T (95.8 %) and Skermanella xinjiangensis 10-1-101T (92.9 %). The DNA–DNA hybridization value between strain SB22T and S. aerolata KACC 11604T ( = 5416T-32T) was 43.3 %. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics of strain SB22T and related species, it is considered that the isolate represents a novel species of the genus Skermanella , for which the name Skermanella stibiiresistens sp. nov. is proposed. The type strain is SB22T ( = CGMCC 1.10751T = KCTC 23364T). An emended description of the genus Skermanella is provided.

scholarly journals Cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus Cribrihabitans Chen et al. 2014

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3897-3903 ◽  
Author(s):  
Asif Hameed ◽  
Mariyam Shahina ◽  
Shih-Yao Lin ◽  
Wei-An Lai ◽  
You-Cheng Liu ◽  
...  
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Surface Seawater ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Unidentified Lipid ◽  
Link Type ◽  
Emended Description

A Gram-stain-negative, strictly aerobic, rod- or oval-shaped, motile, non-spore-forming bacterium, designated strain CC-AMHB-3T, was isolated from coastal surface seawater off Hualien, Taiwan. The novel strain showed high pairwise 16S rRNA gene sequence similarities to Ruegeria mobilis NBRC 101030T (96.5 %), Ruegeria scottomollicae LMG 24367T (96.4 %), Phaeobacter aquaemixtae SSK6-1T (96.2 %), Phaeobacter daeponensis TF-218T (96.2 %), Cribrihabitans marinus CZ-AM5T (96.1 %) and other species of the family Rhodobacteraceae (≤95.9 %). However, strain CC-AMHB-3T formed a distinct phyletic lineage associated with C. marinus CZ-AM5T during phylogenetic analyses. The polar lipid profile of strain CC-AMHB-3T included major amounts of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine; moderate amounts of phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid; and trace amounts of an unidentified lipid and an unidentified phospholipid, which was qualitatively almost in line with that of C. marinus CZ-AM5T but remarkably distinct as compared with the type species of the genera Ruegeria ( Ruegeria atlantica JCM 21234T) and Phaeobacter ( Phaeobacter gallaeciensis JCM 21319T). In line with the fatty acid profile of C. marinus CZ-AM5T, the major (>5 % of total) fatty acids of strain CC-AMHB-3T were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), 11-methyl C18 : 1ω7c and C16 : 0. The DNA G+C content was 66.7 mol%. Ubiquinone-10 (Q-10) was the sole respiratory quinone. Thus, based on the results of the polyphasic study presented here, strain CC-AMHB-3T is considered to represent a novel species of the genus Cribrihabitans , for which the name Cribrihabitans neustonicus sp. nov. is proposed; the type strain is CC-AMHB-3T ( = JCM 19537T = BCRC 80695T). In addition, an emended description of the genus Cribrihabitans is also proposed.

Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1235-1240 ◽  
Author(s):  
Tong Yu ◽  
Qi Yin ◽  
Xiangyu Song ◽  
Rui Zhao ◽  
Xiaochong Shi ◽  
...  
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Gliding Motility ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated SW024T, was isolated from surface seawater of the South Pacific Gyre (26° 29′ S 137° 56′ W) during the Integrated Ocean Drilling Program Expedition 329. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina muelleri KMM 6020T, Aquimarina macrocephali JAMB N27T, Aquimarina addita JC2680T, Aquimarina mytili PSC33T, Aquimarina intermedia KMM 6258T, Aquimarina latercula ATCC 23177T, Aquimarina spongiae A6T, Aquimarina agarilytica ZC1T and Aquimarina brevivitae SMK-19T (96.1, 95.5, 95.0, 94.4, 94.3, 94.0, 93.4, 93.3 and 93.2 % similarities, respectively), demonstrating that the novel strain belonged to the genus Aquimarina . The DNA G+C content of strain SW024T was 30.8 mol%. The major respiratory quinone of strain SW024T was MK-6. The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, C16 : 0 10-methyl and/or iso-C17 : 1ω9c, and iso-C15 : 0 3-OH. The polar lipids of strain SW024T were phosphatidylethanolamine, three unknown aminolipids and five unknown polar lipids. On the basis of combined phenotypic and phylogenetic analyses, strain SW024T represents a novel species of the genus Aquimarina , for which the name Aquimarina longa sp. nov. is proposed. The type strain is SW024T ( = CGMCC 1.11007T = JCM 17859T). An emended description of A. muelleri is also proposed.

Neptuniibacter halophilus sp. nov., isolated from a salt pan, and emended description of the genus Neptuniibacter

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1104-1109 ◽  
Author(s):  
Ming-Hui Chen ◽  
Shih-Yi Sheu ◽  
Tzu-Fang Chiu ◽  
Wen-Ming Chen
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Amino Acid Sequences ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Salt Pan

A bacterial strain designated antisso-13T was isolated from a salt pan in southern Taiwan and characterized using a polyphasic taxonomic approach. Strain antisso-13T was Gram-negative, aerobic, creamy white in colour, rod-shaped and motile by single monopolar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-13T belonged to the genus Neptuniibacter and its closest neighbour was Neptuniibacter caesariensis MED92T, with 96.7 % sequence similarity. Phylogenetic analyses based on rpoB and recA gene sequences and deduced amino acid sequences also revealed that the novel strain and N. caesariensis MED92T formed a distinct phylogenetic cluster. Strain antisso-13T exhibited optimal growth at 25–30 °C, with 2.0–4.0 % NaCl and at pH 7.0. Cellular fatty acids were C16 : 1ω7c (39.8 %), C18 : 1ω7c (29.4 %), C16 : 0 (15.5 %), C10 : 0 3-OH (7.5 %), anteiso-C11 : 0 (1.8 %), C18 : 0 (1.8 %), C11 : 0 2-OH (1.6 %), iso-C10 : 0 (1.2 %) and C14 : 0 (1.1 %). The major respiratory quinone was ubiquinone Q-8. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine and several uncharacterized polar lipids and the DNA G+C content was 54.2 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from previously described members of the genus Neptuniibacter . It is evident from the genotypic and phenotypic data that strain antisso-13T should be classified as a representative of a novel species in the genus Neptuniibacter . The name proposed for this taxon is Neptuniibacter halophilus sp. nov.; the type strain is antisso-13T ( = LMG 25378T = BCRC 80079T). An emended description of the genus Neptuniibacter is provided.

scholarly journals Granulosicoccus marinus sp. nov., isolated from Antarctic seawater, and emended description of the genus Granulosicoccus

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4103-4108 ◽  
Author(s):  
Kiwoon Baek ◽  
Ahyoung Choi ◽  
Ilnam Kang ◽  
Mihye Im ◽  
Jang-Cheon Cho
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Dna Relatedness ◽  
Link Type ◽  
Emended Description

A Gram-staining-negative, motile by flagella, non-pigmented, poly-β-hydroxybutyrate-producing, strictly aerobic and sphere-shaped bacterium, IMCC3490T, was isolated from a coastal seawater sample from the Antarctic Peninsula. Optimal growth of strain IMCC3490T was observed at 20 °C, pH 8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain IMCC3490T belonged to the genus Granulosicoccus in the family Granulosicoccaceae . The strain was closely related to Granulosicoccus antarcticus IMCC3135T (98.8 % 16S rRNA gene sequence similarity) and Granulosicoccus coccoides Z 271T (98.5 %). The DNA–DNA relatedness values between IMCC3490T and type strains of the two species of the genus were far lower than 70 %, which indicated strain IMCC3490T is a novel genomic species of the genus Granulosicoccus . The major fatty acids of strain IMCC3490T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The isoprenoid quinone detected was ubiquinone-8 (Q-8) and predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 61.0 mol%. On the basis of phylogenetic analyses, DNA–DNA relatedness values and phenotypic data, it is suggested that strain IMCC3490T represents a novel species of the genus Granulosicoccus , for which the name Granulosicoccus marinus sp. nov. is proposed. The type strain is IMCC3490T ( = KACC 17483T = NBRC 109704T). An emended description of the genus Granulosicoccus is also provided.

Olleya namhaensis sp. nov., isolated from wood falls, and emended description of the genus Olleya Mancuso Nichols et al. 2005 emend. Lee et al. 2010

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1610-1615 ◽  
Author(s):  
Mi-Hwa Lee ◽  
Yong-Taek Jung ◽  
Sooyeon Park ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A Gram-stain-negative, non-flagellated, gliding, rod-shaped bacterial strain, designated WT-MY15T, was isolated from wood falls in the South Sea in Korea and subjected to a polyphasic taxonomic study. Strain WT-MY15T grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WT-MY15T clustered with the type strains of two Olleya species, exhibiting 16S rRNA gene sequence similarity values of 97.7–98.1 %. Strain WT-MY15T contained MK-6 as the predominant menaquinone. The fatty acid and polar lipid profiles of strain WT-MY15T were similar to those of Olleya aquimaris L-4T and Olleya marilimosa CIP 108537T. The DNA G+C content of strain WT-MY15T was 42.8 mol% and its mean DNA–DNA relatedness values with O. aquimaris L-4T and O. marilimosa CIP 108537T were 8.3 and 5.6 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WT-MY15T is separate from the two recognized species of the genus Olleya . On the basis of the data presented, strain WT-MY15T is considered to represent a novel species of the genus Olleya , for which the name Olleya namhaensis sp. nov. is proposed. The type strain is WT-MY15T ( = KCTC 23673T = CCUG 61507T). An emended description of the genus Olleya is also presented.

Pseudomonas guangdongensis sp. nov., isolated from an electroactive biofilm, and emended description of the genus Pseudomonas Migula 1894

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4599-4605 ◽  
Author(s):  
Guiqin Yang ◽  
Luchao Han ◽  
Junlin Wen ◽  
Shungui Zhou
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A Gram-negative, straight to slightly curved rod-shaped bacterium, motile with peritrichous flagella, designated SgZ-6T, was isolated from an electroactive biofilm and was characterized by means of a polyphasic approach. Growth occurred with 0–5.0 % (w/v) NaCl (optimum 1 %), at pH 6.0–10.0 (optimum pH 7.0) and at 10–42 °C (optimum 30 °C) in trypticase soya broth. Phylogenetic analyses based on the 16S rRNA and gyrB genes identified the isolate as a member of a novel species of the genus Pseudomonas . Strain SgZ-6T exhibited the highest 16S rRNA gene sequence similarity to ‘ Pseudomonas linyingensis’ CGMCC 1.10701 (97.5 %), followed by Pseudomonas sagittaria JCM 18195T (97.4 %), P. oleovorans subsp. lubricantis DSM 21016T (96.6 %), P. tuomuerensis JCM 14085T (96.5 %) and P. alcaliphila JCM 10630T (96.4 %). Strain SgZ-6T showed the highest gyrB gene sequence similarity of 93.7 % to ‘P. linyingensis’ CGMCC 1.10701 among all type strains of genus Pseudomonas . DNA–DNA pairing studies showed that strain SgZ-6T displayed 47.1 and 40.3 % relatedness to ‘P. linyingensis’ CGMCC 1.10701 and P. sagittaria JCM 18195T, respectively. The major isoprenoid quinone was ubiquinone 9 (Q-9). The whole-cell fatty acids consisted mainly of summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The DNA G+C content of the genomic DNA was 68.1 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain SgZ-6T is proposed to represent a novel species of the genus Pseudomonas , for which the name Pseudomonas guangdongensis sp. nov. is proposed. The type strain is SgZ-6T ( = CCTCC AB 2012022T = KACC 16606T). An emended description of the genus Pseudomonas is also proposed.

Cyclobacterium caenipelagi sp. nov., isolated from a tidal flat sediment, and emended description of the genus Cyclobacterium

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3158-3163 ◽  
Author(s):  
Yong-Taek Jung ◽  
Jung-Sook Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment ◽  
Emended Description

A Gram-staining-negative, aerobic, non-flagellated, non-gliding and ring-like or horseshoe-shaped bacterial strain, designated HD-17T, was isolated from a tidal flat sediment in the Korean peninsula. Strain HD-17T grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HD-17T fell within the clade comprising species of the genus Cyclobacterium . Strain HD-17T exhibited 16S rRNA gene sequence similarity values of 93.8–98.8 % to the type strains of species of the genus Cyclobacterium . Strain HD-17T contained MK-7 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), anteiso-C15 : 0 and iso-C15 : 1 G as the major fatty acids. The major polar lipids of strain HD-17T were phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HD-17T was 43.8 mol% and its DNA–DNA relatedness values with Cyclobacterium amurskyense KCTC 12363T, Cyclobacterium qasimii KCTC 23011T and Cyclobacterium marinum KCTC 2917T were 10.4, 7.6 and 5.3 %, respectively. The phylogenetic and genetic distinctiveness and several differentiating phenotypic properties revealed that strain HD-17T was separate from other species of the genus Cyclobacterium . On the basis of the data presented, strain HD-17T represents a novel species of the genus Cyclobacterium , for which the name Cyclobacterium caenipelagi sp. nov. is proposed. The type strain is HD-17T ( = KCTC 32178T = CCUG 63247T). An emended description of the genus Cyclobacterium is also provided.

scholarly journals Microbacterium algeriense sp. nov., a novel actinobacterium isolated from Algerian oil production waters

2020 ◽  
Vol 70 (12) ◽  
pp. 6044-6051 ◽  
Author(s):  
Nesrine Lenchi ◽  
Adriana Anzil ◽  
Pierre Servais ◽  
Salima Kebbouche-Gana ◽  
Mohamed Lamine Gana ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Draft Genome ◽  
Trna Genes ◽  
Rrna Gene ◽  
Protein Coding ◽  
Content Type ◽  
Link Type ◽  
Straight Rod

A non-motile, straight-rod-shaped, Gram-stain-positive and facultative anaerobic bacterium (i.e., strain G1T) was isolated from production waters from an Algerian oilfield. Growth was observed in the presence of 0.3–3.5 % (w/v) NaCl, at 20–50 °C and at pH 6.0–9.0. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that strain G1T belonged to the genus Microbacterium . Strain G1 T was closely related to Microbacterium oxydans (DSM 20578T) and Microbacterium maritypicum (DSM 12512T) with 99.8 % sequence similarity and to Microbacterium saperdae (DSM 20169T) with 99.6 % sequence similarity. Strain G1 T contained MK9, MK10, MK11, MK12 and MK13 as respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol and glycolipid as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The estimated DNA G+C content was 69.57 mol% based on its draft genome sequence. Genome annotation of strain G1T predicted the presence of 3511 genes, of which 3483 were protein-coding and 47 were tRNA genes. The DNA–DNA hybridization (DDH) and average nucleotide identity (ANI) values between strain G1T and M. oxydans (DSM 20578T) and M. maritypicum (DSM 12512T) were in both cases far below the respective species boundary thresholds (27.5 and 28.0 % for DDH; and 84.40 and 84.82% for ANI, respectively). Based on the data presented above, strain G1T was considered to represent a novel species for which the name Microbacterium algeriense is proposed with the type strain G1T (=DSM 109018T=LMG 31276T).

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