scholarly journals Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera

2020 ◽  
Vol 70 (7) ◽  
pp. 4321-4328
Author(s):  
Cristina Torcato ◽  
Micael F. M. Gonçalves ◽  
Edgar Rodríguez-Gálvez ◽  
Artur Alves
Keyword(s):  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
The Other ◽  
Translation Elongation Factor ◽  
Sequence Analyses ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef-1α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus Clonostachys. Single- and multi-locus gene phylogenies confirmed the isolates as representing members of the genus Clonostachys, more closely related to species in the subgenus Bionectria. In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species Clonostachys pseudochroleuca and the other one distinct from all known species of the genus Clonostachys. These isolates are recognized as representing a novel species species for which the name Clonostachys viticola is proposed.

scholarly journals Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

MycoKeys ◽  
2021 ◽  
Vol 80 ◽  
pp. 77-90
Author(s):  
Xianhong Wang ◽  
Yashuang Guo ◽  
Yamin Du ◽  
Ziling Yang ◽  
Xinzhong Huang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Elongation Factor 1

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

Diaporthe sinensis, a new fungus from Amaranthus sp. in China 

Phytotaxa ◽  
2019 ◽  
Vol 425 (5) ◽  
pp. 259-268
Author(s):  
XIAO-XIAO FENG ◽  
JIA-JIE CHEN ◽  
GUO-RONG WANG ◽  
TING-TING CAO ◽  
YONG-LI ZHENG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Plant Pathogens ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Fungal Species ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Stems And Leaves ◽  
Elongation Factor 1 ◽  
Β Tubulin

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.

Saprobic Dothideomycetes in Thailand: Phaeoseptum hydei sp. nov., a new terrestrial ascomycete in Phaeoseptaceae

Phytotaxa ◽  
2020 ◽  
Vol 449 (2) ◽  
pp. 149-163
Author(s):  
DHANUSHKA N. WANASINGHE ◽  
PETER E. MORTIMER ◽  
CHANOKNED SENWANNA ◽  
RATCHADAWAN CHEEWANGKOON
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
University Campus ◽  
Translation Elongation ◽  
Delonix Regia ◽  
The Family ◽  
Elongation Factor 1 Alpha ◽  
Vertical Septum ◽  
Elongation Factor 1

During a survey of saprobic microfungi in Thailand, a dothideomycetous fungus was found on a dead twig of Delonix regia, on the Chiang Mai University campus. This fungus is characterized by fully immersed ascomata under a small blackened pseudoclypeus, pseudoparenchymatous peridium, cellular pseudoparaphyses, cylindrical-clavate asci with a distinct pedicel, overlapping 3–4-seriate, pale to dark brown, broadly fusoid, 7–9-transversally septate ascospores with a vertical septum in nearly all median cells. Multigene phylogenetic analyses, using partial sequences from the 28S nrRNA gene (LSU), 18S nrRNA gene (SSU), internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon and the translation elongation factor 1-alpha region (TEF) demonstrated a monophyletic affiliation of the new strain, accommodating the species of Phaeoseptum in the family Phaeoseptaceae. With further morphological and phylogenetic investigations, we justify the new fungus as a novel species, Phaeoseptum hydei in Phaeoseptaceae. Detailed descriptions and illustrations are provided for Phaeoseptum hydei and this novel species compared with the remaining species found in the genus. An updated checklist of microfungi recorded on Delonix regia from around the world is also provided.

Additions to Karst Fungi 5: Sardiniella guizhouensis sp. nov. (Botryosphaeriaceae) associated with woody hosts in Guizhou province, China

Phytotaxa ◽  
2021 ◽  
Vol 508 (2) ◽  
Author(s):  
YA-YA CHEN ◽  
ASHA J. DISSANAYAKE ◽  
JIAN-KUI LIU
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Guizhou Province ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Distinct Lineage ◽  
Elongation Factor 1 ◽  
A New Species

During an investigation of ascomycetous fungi in Karst formations of the Asian region, three interesting taxa were found on dead aerial stems of woody hosts in Guizhou province, China. Their morphology assigned them as typical botryosphaeriaceous species. Phylogenetic analyses based on a combined DNA dataset of large subunit (LSU), internal transcribed spacer (ITS) and part of the translation elongation factor 1-α (tef1) gene confirmed their placement within Botryosphaeriaceae. In the phylogenetic tree, the three isolates formed a well-supported monotypic clade as a distinct lineage within the genus Sardiniella. Therefore, a new species Sardiniella guizhouensis sp. nov. is introduced to accommodate these taxa and detailed, illustrated descriptions of the asexual and sexual morphs are provided. This study reveals the first sexual morph of Sardiniella, which it is characterized by 2–4(–6)-spored asci with hyaline to brown, aseptate to 1-septate, ovate to subclavate ascospores.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

Pestalotiopsis anacardiacearum sp. nov. (Amphisphaeriaceae) has an intricate relationship with Penicillaria jocosatrix the mango tip borer

Phytotaxa ◽  
2013 ◽  
Vol 99 (2) ◽  
pp. 49 ◽  
Author(s):  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
YANMIN ZHANG ◽  
YONG WANG ◽  
KEVIN D. HYDE
Keyword(s):  
Internal Transcribed Spacer ◽  
Yunnan Province ◽  
Mangifera Indica ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
Partial Translation ◽  
Β Tubulin

Pestalotiopsis anacardiacearum sp. nov. is described from leaves of Mangifera indica from Yunnan Province, China. The taxon can clearly be distinguished from all known species of Pestalotiopsis by its morphology. Phylogenetic analysis based on combined multi-locus alignment of the internal transcribed spacer (ITS), partial β-tubulin and partial translation elongation factor 1-alpha (tef1) also distinguishes this taxon. It can be distinguished from previously recorded Pestalotiopsis pathogens on mango by having larger conidia. The species occurs on leaves of mango following death associated with the mango tip borer (Penicillaria jocosatrix).

Cladosporium omanense, a new endophytic species from Zygophyllum coccineum in Oman

Phytotaxa ◽  
2019 ◽  
Vol 388 (1) ◽  
pp. 145 ◽  
Author(s):  
BOSHRA A. HALO ◽  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
RASHID A. AL-YAHYAI ◽  
ABDULLAH M. AL-SADI
Keyword(s):  
Sequence Data ◽  
Novel Species ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Cladosporium Sphaerospermum ◽  
Elongation Factor 1 Alpha ◽  
Desert Habitat ◽  
Elongation Factor 1 ◽  
Partial Translation

The genus Cladosporium is cosmopolitan in distribution and can be isolated from various sources including plants as endophytes. A strain of Cladosporium was isolated from living leaves of Zygophyllum coccineum in a desert habitat in Oman. Phylogenetic analysis based on the combined internal transcribed spacer (ITS), partial translation elongation factor 1-alpha (TEF) and partial actin (ACT) sequence data shows that this is a novel species, which groups with Cladosporium sphaerospermum complex. The new species is introduced as Cladosporium omanense and is described, illustrated and compared with other known species in the genus. Cladosporium omanense differs from its phylogenetically related species C. endophyticum by its fast growing colonies, longer macronematous and micronematous conidiophores, thickened-walled conidia with conspicuously protuberant shrivelling surface ornamentations.

scholarly journals Novel species of Cladosporium from environmental sources in Spain

MycoKeys ◽  
2021 ◽  
Vol 77 ◽  
pp. 1-25
Author(s):  
Isabel Iturrieta-González ◽  
Dania García ◽  
Josepa Gené
Keyword(s):  
Internal Transcribed Spacer ◽  
Plant Material ◽  
Species Complex ◽  
Novel Species ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Human Species ◽  
Translation Elongation ◽  
Morphological Differences ◽  
Elongation Factor 1

Cladosporium is a monophyletic genus in Cladosporiaceae (Cladosporiales, Dothideomycetes) whose species are mainly found as saprobes and endophytes, but it also includes fungi pathogenic for plants, animals and human. Species identification is currently based on three genetic markers, viz., the internal transcribed spacer regions (ITS) of the rDNA, and partial fragments of actin (act) and the translation elongation factor 1-α (tef1) genes. Using this phylogenetic approach and from morphological differences, we have recognized six new species originating from soil, herbivore dung and plant material collected at different Spanish locations. They are proposed as Cladosporium caprifimosum, C. coprophilum, C. fuscoviride and C. lentulum belonging in the C. cladosporioides species complex, and C. pseudotenellum and C. submersum belonging in the C. herbarum species complex. This study revealed that herbivore dung represented a reservoir of novel lineages in the genus Cladosporium.

New Endophytic Fusarium spp. from Fraxinus excelsior Leaves in Slovakia

Planta Medica ◽  
2020 ◽  
Vol 86 (13/14) ◽  
pp. 1025-1031
Author(s):  
Helena Ivanova ◽  
Lenka Malinicova ◽  
Maria Piknova ◽  
Peter Pristas
Keyword(s):  
Internal Transcribed Spacer ◽  
Elongation Factor ◽  
Fraxinus Excelsior ◽  
Translation Elongation Factor ◽  
Fusarium Spp ◽  
Translation Elongation ◽  
Ash Dieback ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
Β Tubulin

Abstract Fraxinus excelsior is currently suffering from ash dieback disease caused by the fungus Hymenoscyphus fraxineus. Co-occurrence of large numbers of other fungi with endophytic as well as pathogenic lifestyles has been observed on F. excelsior. In this study, new endophytic Fusarium spp. isolate Fraxinus excelsior Fusarium from Slovak territory, associated with F. excelsior, was characterised. Using morphology-based approaches, the fungus was assigned to the genus Fusarium. The isolate formed thick-walled hyaline, fusiform, slightly curved macroconidia, produced from monophialides in sporodochia, pointed at the tip, mostly 3 – 5 septate, occasionally 6 septate, and 40 – 68 × 3.8 – 5.0 µm in size with basal pedicellate cells. For more accurate taxonomic affiliation, molecular sequence analyses of the internal transcribed spacer, translation elongation factor 1-alpha sequences, and partial β-tubulin gene were performed. While the internal transcribed spacer sequence of the isolate showed the highest similarity to the Fraxinus oxysporum species, translation elongation factor 1-alpha and partial β-tubulin sequences were distantly related to the Fraxinus avenaceum species. Based on these data, the Fraxinus excelsior Fusarium isolate could probably be considered a new species of the Fusarium genus.

Sign in / Sign up

Export Citation Format

Share Document