Characterization of the translation elongation factor 1-α gene in a wide range of pathogenic Aspergillus species

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

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Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China

Phytotaxa ◽

10.11646/phytotaxa.269.2.2 ◽

2016 ◽

Vol 269 (2) ◽

pp. 90 ◽

Cited By ~ 11

Author(s):

ZHUO DU ◽

XIN-LEI FAN ◽

KEVIN D. HYDE ◽

QIN YANG ◽

YING-MEI LIANG ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Sequence Data ◽

Histone H3 ◽

Elongation Factor ◽

Betula Platyphylla ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Wide Range ◽

Dieback Disease ◽

Elongation Factor 1

Diaporthe species are common pathogens, endophytes, or saprobes on a wide range of hosts. During our investigation of forest pathogens, we made collections of Diaporthe species associated with canker and dieback disease of Betula platyphylla and B. albosinensis in Sichuan and Shaanxi provinces in China. Diaporthe betulae sp. nov. and D. betulicola sp. nov. are introduced in this paper, with illustrations, descriptions and support from analysis of ribosomal DNA internal transcribed spacer (ITS), calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-α (TEF1-α) and beta-tubulin (TUB2) sequence data. Diaporthe betulae is characterized by hyaline, ellipsoidal, aseptate, biguttulate, 8.5–11 × 3–4 µm alpha conidia. Diaporthe betulicola is characterized by pycnidial stromata with a single locule with one ostiole per disc. Alpha conidia are hyaline, oblong, aseptate, lack guttules and 9.9–14.7 × 1.3–2.5 µm, and beta conidia are hyaline, spindle-shaped, curved, aseptate and 17–24 × 0.7–1.2 µm.

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In vivo characterization of the role of tissue‐specific translation elongation factor 1 A 2 in protein synthesis reveals insights into muscle atrophy

FEBS Journal ◽

10.1111/febs.12554 ◽

2013 ◽

Vol 280 (24) ◽

pp. 6528-6540 ◽

Cited By ~ 13

Author(s):

Jennifer Doig ◽

Lowri A. Griffiths ◽

David Peberdy ◽

Permphan Dharmasaroja ◽

Maria Vera ◽

...

Keyword(s):

Protein Synthesis ◽

Muscle Atrophy ◽

Elongation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

In Vivo Characterization ◽

Elongation Factor 1

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Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽

10.1094/pdis-01-18-0171-re ◽

2018 ◽

Vol 102 (11) ◽

pp. 2083-2100 ◽

Cited By ~ 4

Author(s):

Beatriz Mora-Sala ◽

Ana Cabral ◽

Maela León ◽

Carlos Agustí-Brisach ◽

Josep Armengol ◽

...

Keyword(s):

Dna Sequences ◽

Elongation Factor ◽

Its Region ◽

Phylogenetic Position ◽

Translation Elongation ◽

Forest Nurseries ◽

Asexual Morphs ◽

Identification And Characterization ◽

Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

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Sequence of a cDNA encoding the α-subunit of wheat translation elongation factor 1

Gene ◽

10.1016/0378-1119(92)90113-4 ◽

1992 ◽

Vol 120 (2) ◽

pp. 315-316 ◽

Cited By ~ 7

Author(s):

Anneke M. Metz ◽

Richard T. Timmer ◽

M.Leah Allen ◽

Karen S. Browning

Keyword(s):

Elongation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Α Subunit ◽

Elongation Factor 1

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Cloning and genetic characterization of a calcium- and phospholipid-binding protein fromSaccharomyces cerevisiae that is homologous to translation elongation factor-1γ

Yeast ◽

10.1002/yea.320090206 ◽

1993 ◽

Vol 9 (2) ◽

pp. 151-163 ◽

Cited By ~ 10

Author(s):

Nicholas G. Kambouris ◽

Daniel J. Burke ◽

Carl E. Creutz

Keyword(s):

Genetic Characterization ◽

Binding Protein ◽

Elongation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Phospholipid Binding

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Global Molecular Diversity of the Halotolerant Fungus Hortaea werneckii

Life ◽

10.3390/life8030031 ◽

2018 ◽

Vol 8 (3) ◽

pp. 31 ◽

Cited By ~ 12

Author(s):

Alessia Marchetta ◽

Bert Gerrits van den Ende ◽

Abdullah Al-Hatmi ◽

Ferry Hagen ◽

Polona Zalar ◽

...

Keyword(s):

Molecular Diversity ◽

Sea Water ◽

Elongation Factor ◽

Clonal Expansion ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Hortaea Werneckii ◽

Halotolerant Fungus ◽

Elongation Factor 1 ◽

Partial Translation

A global set of clinical and environmental strains of the halotolerant black yeast-like fungus Hortaea werneckii are analyzed by multilocus sequencing and AFLP, and physiological parameters are determined. Partial translation elongation factor 1-α proves to be suitable for typing because of the presence/absence of introns and also the presence of several SNPs. Local clonal expansion could be established by a combination of molecular methods, while the population from the Mediterranean Sea water also responds differently to combined temperature and salt stress. The species comprises molecular populations, which in part also differ physiologically allowing further diversification, but clinical strains did not deviate significantly from their environmental counterparts.

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Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization inIris. lacteavar.chinensisRoots under Cadmium, Lead, and Salt Stress Conditions

The Scientific World JOURNAL ◽

10.1155/2014/532713 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Chun-Sun Gu ◽

Liang-qin Liu ◽

Chen Xu ◽

Yan-hai Zhao ◽

Xu-dong Zhu ◽

...

Keyword(s):

Gene Expression ◽

Salt Stress ◽

Real Time ◽

Reference Genes ◽

Elongation Factor ◽

Stress Conditions ◽

Translation Initiation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Wide Range

Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples. In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1α), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), inIris. lacteavar.chinensis(I. lacteavar.chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions. All seven reference genes showed a relatively wide range of threshold cycles (Ct) values in different samples. GeNorm and NormFinder algorithms were used to assess the suitable reference genes. The results from the two software units showed thatEIF-5AandUBCwere the most stable reference genes across all of the tested samples, whileTUBLINwas unsuitable as internal controls.I. lacteavar.chinensisis tolerant to Cd, Pb, and salt. Our results will benefit future research on gene expression in response to the three abiotic stresses.

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Diaporthe sinensis, a new fungus from Amaranthus sp. in China

Phytotaxa ◽

10.11646/phytotaxa.425.5.1 ◽

2019 ◽

Vol 425 (5) ◽

pp. 259-268

Author(s):

XIAO-XIAO FENG ◽

JIA-JIE CHEN ◽

GUO-RONG WANG ◽

TING-TING CAO ◽

YONG-LI ZHENG ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Plant Pathogens ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Fungal Species ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Stems And Leaves ◽

Elongation Factor 1 ◽

Β Tubulin

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.

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Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines

Plant Disease ◽

10.1094/pdis-03-15-0286-re ◽

2015 ◽

Vol 99 (12) ◽

pp. 1678-1688 ◽

Cited By ~ 16

Author(s):

Antonia Carlucci ◽

Francesca Cibelli ◽

Francesco Lops ◽

Maria Luisa Raimondo

Keyword(s):

Elongation Factor ◽

Phylogenetic Study ◽

Translation Elongation ◽

Neofusicoccum Parvum ◽

Grapevine Trunk Diseases ◽

General Decline ◽

Wide Range ◽

Trunk Diseases ◽

Pathogenicity Tests

Botryosphaeriaceae spp. have a cosmopolitan distribution and a wide range of plant hosts. Over the last 15 years, worldwide, 21 species of this family have been associated with grapevine trunk diseases that cause cankers and dieback on grapevines. Here, we surveyed vineyards of Vitis vinifera ‘Lambrusco’, ‘Sangiovese’, and ‘Montepulciano’ in three areas of the Foggia province (Cerignola, Foggia, and San Severo) in southern Italy. Wood samples from grapevines showing general decline, dieback, cankers, and wood and foliar discoloration yielded 344 fungal isolates identified as Botryosphaeriaceae spp. A phylogenetic study combining internal transcribed spacer and translation elongation factor 1-α sequences of 60 representative isolates identified nine botryosphaeriaceous species: Botryosphaeria dothidea, Diplodia corticola, D. mutila, D. seriata, Dothiorella iberica, Do. sarmentorum, Lasiodiplodia citricola, L. theobromae, and Neofusicoccum parvum. Pathogenicity tests confirmed that all nine species cause canker and dieback of grapevines. However, this is the first report of L. citricola as causal agent of wood cankers and dieback of grapevine. To date, including L. citricola, there are 25 botryosphaeriaceous species associated with V. vinifera worldwide, of which 12 have been reported for grapevines in Italy.

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