Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China

Phytotaxa ◽  
2016 ◽  
Vol 269 (2) ◽  
pp. 90 ◽  
Author(s):  
ZHUO DU ◽  
XIN-LEI FAN ◽  
KEVIN D. HYDE ◽  
QIN YANG ◽  
YING-MEI LIANG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Histone H3 ◽  
Elongation Factor ◽  
Betula Platyphylla ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Dieback Disease ◽  
Elongation Factor 1

Diaporthe species are common pathogens, endophytes, or saprobes on a wide range of hosts. During our investigation of forest pathogens, we made collections of Diaporthe species associated with canker and dieback disease of Betula platyphylla and B. albosinensis in Sichuan and Shaanxi provinces in China. Diaporthe betulae sp. nov. and D. betulicola sp. nov. are introduced in this paper, with illustrations, descriptions and support from analysis of ribosomal DNA internal transcribed spacer (ITS), calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-α (TEF1-α) and beta-tubulin (TUB2) sequence data. Diaporthe betulae is characterized by hyaline, ellipsoidal, aseptate, biguttulate, 8.5–11 × 3–4 µm alpha conidia. Diaporthe betulicola is characterized by pycnidial stromata with a single locule with one ostiole per disc. Alpha conidia are hyaline, oblong, aseptate, lack guttules and 9.9–14.7 × 1.3–2.5 µm, and beta conidia are hyaline, spindle-shaped, curved, aseptate and 17–24 × 0.7–1.2 µm.

Three new species of Diaporthe from China based on morphological characters and DNA sequence data analyses

Phytotaxa ◽  
2019 ◽  
Vol 422 (2) ◽  
pp. 157-174 ◽  
Author(s):  
HAO ZHOU ◽  
CHENG-LIN HOU
Keyword(s):  
Sequence Data ◽  
Histone H3 ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Dna Sequence Data ◽  
Rdna Its ◽  
Three New Species ◽  
Elongation Factor 1

In the present study, nine endophytic strains of Diaporthe from China were identified based on analyses of morphological characters and combined sequences of rDNA ITS, partial sequences from the translation elongation factor 1-α (tef1), β tubulin (tub2), histone H3 (his3), and calmodulin (cal) genes. Three new Diaporthe species, Diaporthe anhuiensis, Diaporthe huangshanensis and Diaporthe shennongjiaensis, are introduced in this paper with full descriptions and comparison with similar taxa. And two other known species, Diaporthe citrichinensis and Diaporthe eres are also described.

scholarly journals Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

MycoKeys ◽  
2021 ◽  
Vol 80 ◽  
pp. 77-90
Author(s):  
Xianhong Wang ◽  
Yashuang Guo ◽  
Yamin Du ◽  
Ziling Yang ◽  
Xinzhong Huang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Elongation Factor 1

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

Diaporthe sinensis, a new fungus from Amaranthus sp. in China 

Phytotaxa ◽  
2019 ◽  
Vol 425 (5) ◽  
pp. 259-268
Author(s):  
XIAO-XIAO FENG ◽  
JIA-JIE CHEN ◽  
GUO-RONG WANG ◽  
TING-TING CAO ◽  
YONG-LI ZHENG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Plant Pathogens ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Fungal Species ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Stems And Leaves ◽  
Elongation Factor 1 ◽  
Β Tubulin

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.

scholarly journals Characterization of the translation elongation factor 1-α gene in a wide range of pathogenic Aspergillus species

2017 ◽  
Vol 66 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Sadegh Nouripour-Sisakht ◽  
Bahram Ahmadi ◽  
Koichi Makimura ◽  
Sybren de Hoog ◽  
Yoshiko Umeda ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Aspergillus Species ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Elongation Factor 1

Additions to Karst Fungi 5: Sardiniella guizhouensis sp. nov. (Botryosphaeriaceae) associated with woody hosts in Guizhou province, China

Phytotaxa ◽  
2021 ◽  
Vol 508 (2) ◽  
Author(s):  
YA-YA CHEN ◽  
ASHA J. DISSANAYAKE ◽  
JIAN-KUI LIU
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Guizhou Province ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Distinct Lineage ◽  
Elongation Factor 1 ◽  
A New Species

During an investigation of ascomycetous fungi in Karst formations of the Asian region, three interesting taxa were found on dead aerial stems of woody hosts in Guizhou province, China. Their morphology assigned them as typical botryosphaeriaceous species. Phylogenetic analyses based on a combined DNA dataset of large subunit (LSU), internal transcribed spacer (ITS) and part of the translation elongation factor 1-α (tef1) gene confirmed their placement within Botryosphaeriaceae. In the phylogenetic tree, the three isolates formed a well-supported monotypic clade as a distinct lineage within the genus Sardiniella. Therefore, a new species Sardiniella guizhouensis sp. nov. is introduced to accommodate these taxa and detailed, illustrated descriptions of the asexual and sexual morphs are provided. This study reveals the first sexual morph of Sardiniella, which it is characterized by 2–4(–6)-spored asci with hyaline to brown, aseptate to 1-septate, ovate to subclavate ascospores.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

Pestalotiopsis anacardiacearum sp. nov. (Amphisphaeriaceae) has an intricate relationship with Penicillaria jocosatrix the mango tip borer

Phytotaxa ◽  
2013 ◽  
Vol 99 (2) ◽  
pp. 49 ◽  
Author(s):  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
YANMIN ZHANG ◽  
YONG WANG ◽  
KEVIN D. HYDE
Keyword(s):  
Internal Transcribed Spacer ◽  
Yunnan Province ◽  
Mangifera Indica ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
Partial Translation ◽  
Β Tubulin

Pestalotiopsis anacardiacearum sp. nov. is described from leaves of Mangifera indica from Yunnan Province, China. The taxon can clearly be distinguished from all known species of Pestalotiopsis by its morphology. Phylogenetic analysis based on combined multi-locus alignment of the internal transcribed spacer (ITS), partial β-tubulin and partial translation elongation factor 1-alpha (tef1) also distinguishes this taxon. It can be distinguished from previously recorded Pestalotiopsis pathogens on mango by having larger conidia. The species occurs on leaves of mango following death associated with the mango tip borer (Penicillaria jocosatrix).

Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 28 ◽  
Author(s):  
KAI CHEN ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Culture Characteristics ◽  
Close Relatives ◽  
Encoding Genes ◽  
Elongation Factor 1

Seven new species of the genus Trichoderma belonging to the Viride clade are described from soil samples of different regions in China, and named as T. adaptatum, T. beijingense, T. bifurcatum, T. mangshanicum, T. shaoguanicum, T. tardum and T. vulgatum. Their phylogenetic positions are determined by analyses of the combined partial sequences of translation elongation factor 1-alpha and the second largest RNA polymerase subunit encoding genes. Morphology and culture characteristics are observed, described and illustrated in detail. All the new species form green conidia, fit nicely the Viride clade, but differ obviously from any known species of the genus in sequence data, colony features, growth rates, and size of phialides and conidia. Distinctions between the new species and their close relatives are discussed.

scholarly journals Endophytic Diaporthe Associated With Citrus grandis cv. Tomentosa in China

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhangyong Dong ◽  
Ishara S. Manawasinghe ◽  
Yinghua Huang ◽  
Yongxin Shu ◽  
Alan J. L. Phillips ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Current Knowledge ◽  
Elongation Factor ◽  
Guangdong Province ◽  
Multigene Phylogeny ◽  
Intensive Study ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1

Diaporthe species are associated with Citrus as endophytes, pathogens, and saprobes worldwide. However, little is known about Diaporthe as endophytes in Citrus grandis in China. In this study, 24 endophytic Diaporthe isolates were obtained from cultivated C. grandis cv. “Tomentosa” in Huazhou, Guangdong Province in 2019. The nuclear ribosomal internal transcribed spacer (ITS), partial sequences of translation elongation factor 1-α (tef1), β-tubulin (tub2), and partial calmodulin (cal) gene regions were sequenced and employed to construct phylogenetic trees. Based on morphology and combined multigene phylogeny, eleven Diaporthe species were identified including two new species, Diaporthe endocitricola and D. guangdongensis. These are the first report of D. apiculata, D. aquatica, D. arecae, D. biconispora, D. limonicola, D. masirevicii, D. passifloricola, D. perseae, and D. sennae on C. grandis. This study provides the first intensive study of endophytic Diaporthe species on C. grandis cv. tomentosa in China. These results will improve the current knowledge of Diaporthe species associated with C. grandis. The results obtained in this study will also help to understand the potential pathogens and biocontrol agents and to develop a platform in disease management.

scholarly journals Morphology, Phylogeny and Pathogenicity of Pestalotioid Species on Camellia oleifera in China

2021 ◽  
Vol 7 (12) ◽  
pp. 1080
Author(s):  
Lingling Li ◽  
Qin Yang ◽  
He Li
Keyword(s):  
Woody Plant ◽  
Sequence Data ◽  
Southern China ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Camellia Oleifera ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Tea-oil tree (Camellia oleifera) is an important edible oil woody plant with a planting area of over 3,800,000 hectares in southern China. Pestalotioid fungi are associated with a wide variety of plants worldwide along with endophytes, pathogens, and saprobes. In this study, symptomatic leaves of C. oleifera were collected from Guangdong, Guangxi, Hainan, Hunan, and Jiangsu Provinces and pestalotioid fungi are characterized based on combined sequence data analyses of internal transcribed spacer (ITS), beta tubulin (tub2), and translation elongation factor 1-alpha (tef-1α) coupled with morphological characteristics. As a result, seven species were confirmed, of which five species are described as new viz. N. camelliae-oleiferae, P. camelliae-oleiferae, P. hunanensis, P. nanjingensis, P. nanningensis, while the other two are reported as known species, viz., N. cubana and N. iberica. Pathogenicity assays showed that all species except for P. nanjingensis developed brown lesions on healthy leaves and P. camelliae-oleiferae showed stronger virulence.

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