In vitro antifungal activity of luliconazole against nondermatophytic moulds

2019 ◽  
Vol 58 (5) ◽  
pp. 703-706 ◽  
Author(s):  
Jun Maeda ◽  
Hiroyasu Koga ◽  
Kou Yuasa ◽  
Daisuke Neki ◽  
Yasuko Nanjoh ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Drug Resistant ◽  
Fusarium Spp ◽  
Microdilution Method ◽  
In Vitro Antifungal Activity

Abstract In vitro antifungal activity of luliconazole against nondermatophytic moulds causing superficial infections was compared with that of five classes of 12 topical and systemic drugs. The minimum inhibitory concentration (MIC) of the drugs against the genera of Neoscytalidium, Fusarium, Aspergillus, Scedosporium, and Alternaria was measured via modified microdilution method. In results, the nondermatophytic moulds were found to be less susceptible to drugs to which Neoscytalidium spp. and Fusarium spp. were typically drug resistant. However, luliconazole was effective against all the genera tested, including afore-mentioned two species, and had the lowest MICs among the drugs tested.

scholarly journals Antifungal Activity of Plumericin and Isoplumericin

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Dharmendra Singh ◽  
Umakant Sharma ◽  
Parveen Kumar ◽  
Yogesh K Gupta ◽  
M. P. Dobhal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Minimum Fungicidal Concentration ◽  
In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

scholarly journals Antifungal activity of thymol against clinical isolates of fluconazole-sensitive and -resistant Candida albicans

2009 ◽  
Vol 58 (8) ◽  
pp. 1074-1079 ◽  
Author(s):  
Na Guo ◽  
Jingbo Liu ◽  
Xiuping Wu ◽  
Xingming Bi ◽  
Rizeng Meng ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Clinical Isolates ◽  
Standard Strain ◽  
Antagonistic Action ◽  
Microdilution Method ◽  
Interaction Intensity

Thymol (THY) was found to have in vitro antifungal activity against 24 fluconazole (FLC)-resistant and 12 FLC-susceptible clinical isolates of Candida albicans, standard strain ATCC 10231 and one experimentally induced FLC-resistant C. albicans S-1. In addition, synergism was observed for clinical isolates of C. albicans with combinations of THY–FLC and THY–amphotericin B (AMB) evaluated by the chequerboard microdilution method. The interaction intensity was determined by spectrophotometry for the chequerboard assay, and the nature of the interactions was assessed using two non-parametric approaches [fractional inhibitory concentration index (FICI) and ΔE models]. The interaction between THY–FLC or THY–AMB in FLC-resistant and -susceptible strains of C. albicans showed a high percentage of synergism by the FICI method and the ΔE method. The ΔE model gave results consistent with FICI, and no antagonistic action was observed in the strains tested.

scholarly journals 7-Chloroquinolin-4-yl Arylhydrazone Derivatives: Synthesis and Antifungal Activity

2011 ◽  
Vol 11 ◽  
pp. 1489-1495 ◽  
Author(s):  
Auri R. Duval ◽  
Pedro H. Carvalho ◽  
Maieli C. Soares ◽  
Daniela P. Gouvêa ◽  
Geonir M. Siqueira ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Rational Design ◽  
Antifungal Agents ◽  
Inhibitory Concentration ◽  
First Line ◽  
Minimum Fungicidal Concentration ◽  
Starting Point ◽  
Line Drug ◽  
In Vitro Antifungal Activity

Fifteen 7-chloro-4-arylhydrazonequinolines have been evaluated for their in vitro antifungal activity against eight oral fungi:Candida albicans, C. parapsilosis, C. lipolytica, C. tropicalis, C. famata, C. glabrata, Rhodutorula mucilaginosa, andR. glutinis. Several compounds exhibited minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) activities comparable with the first-line drug fluconazole. These results could be considered as an important starting point for the rational design of new antifungal agents.

scholarly journals In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans

2018 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Mariana Lourenço ◽  
Adilson Cesar Abreu Bernardi ◽  
Nadia Lunardi ◽  
Rodolfo Jorge Boeck Neto ◽  
Patricia Sigilló Mazzoni Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Ocimum Basilicum ◽  
Coriandrum Sativum ◽  
Microdilution Method

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

scholarly journals Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]

2019 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Rahayu Kusdarwati, Ayu Ratnaningtyas, Dewa Ketut Meles
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Fresh Water Fish ◽  
Minimum Fungicidal Concentration ◽  
Kaempferia Galanga ◽  
Activity Test

Abstract Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.

scholarly journals IN VITRO ANTIFUNGAL ACTIVITY OF TIMOL AND CARVACROL ON CANDIDA SPECIES

2019 ◽  
Vol 4 ◽  
Author(s):  
Luciana Thaís Rangel Souza ◽  
Cecília Correia Costa ◽  
Mateus Cardoso Oliveira ◽  
Isabel Celeste Caires Pereira Gusmão
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Candida Krusei ◽  
Minimum Fungicidal Concentration ◽  
Sabouraud Agar ◽  
In Vitro Antifungal Activity

Aim: to evaluate the in vitro action of thymol and carvacrol against the yeasts of Candida albicans ATCC10231 and Candida krusei ATCC34135. Method: A laboratory study was performed to evaluate antifungal activity. The characterization of the Minimal Inhibitory Concentration (MIC) of the thymol essential oil was carried out using the technique where the microdilution is performed, in which a plate containing 96 wells is used. The determination of the Minimum Fungicidal Concentration (MFC) was performed by dripping 10 μL of each of the concentrations evaluated on Sabouraud agar plates. Results: The MIC of thymol and carvacrol for C. albicans was 40 μg/mL and for Candida krusei it did not present antifungal activity. While the MIC of nystatin was 0.03mg for both species with thymol and carvacrol. Conclusion: Thymol presented satisfactory antifungal activity against the pathogens studied, but carvacrol did not present antifungal activity.

scholarly journals Stereospecific ligands and their complexes, Part VIII: Antimicrobial activity of palladium(II) complexes with 0,0'-dialkyl esters of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)-pentanoic acid

2012 ◽  
Vol 66 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Dragoslav Ilic ◽  
Jelena Vujic ◽  
Ivana Radojevic ◽  
Olgica Stefanovic ◽  
Ljiljana Comic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
General Formula ◽  
Inhibitory Concentration ◽  
Pentanoic Acid ◽  
In Vitro Antimicrobial Activity ◽  
Microdilution Method ◽  
Vis Spectroscopy ◽  
Bacteria And Fungi

Palladium(II) complexes (1-4) of general formula [PdCl2(R2-S,S-eddp)] with bidentate N,N?-ligands, O,O'-dialkyl esters (R = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N?-di-2-(4-methyl)pentanoic acid (S,S-eddp) were prepared and characterized by microanalysis, infrared and UV/VIS spectroscopy. The ligands and its complexes were tested for their in vitro antimicrobial activity against 15 species of bacteria and fungi. Testing is performed by the microdilution method, with the minimum inhibitory concentration (MIC) and the minimum microbicidal concentration (MMC) being determined. The MIC values were in range from 4.9 ?g cm-3 to > 5000 ?g cm-3 while MMC values ranged from 78 ?g cm-3 to > 5000 ?g cm-3. Palladium(II) complexes [PdCl2(Ln)] (n = 1-4) have statistically significant higher activity than the corresponding ligands. The complex 4 displayed the strongest activity among all tested compounds.

scholarly journals ANTIFUNGAL ACTIVITY OF THE ASSOCIATION OF CARVACROL WITH AMPHOTERICIN B OR WITH KETOCONAZOLE

2019 ◽  
Vol 16 (31) ◽  
pp. 12-17
Author(s):  
Gustavo Lima SOARES ◽  
Brenda Lavínia Calixto dos SANTOS ◽  
Brenna Ravena Araújo LUZ ◽  
Wylly Araújo de OLIVEIRA
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Fungal Infections ◽  
Antifungal Drugs ◽  
Aspergillus Species ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Antifungal Action

Aspergillus species are a cause of a high number of fungal infections of difficult treatment, presenting an expressive number of deaths due to the complications in the severe cases of infection. The objective was to evaluate the antifungal action of carvacrol against Aspergillus species, as well as to evaluate the interactions when associated with amphotericin B or ketoconazole. The antifungal activity of carvacrol was evaluated by the broth microdilution method. The combinations of the substances were performed by the checkerboard methodology, to determine the Index of Fractional Inhibitory Concentration. Carvacrol showed antifungal activity against all Aspergillus strains used in the trials. In combinations of substances, only a combination of carvacrol and amphotericin B presented satisfactory results. Combinations of carvacrol and ketoconazole have not shown good. It is concluded that carvacrol is a good candidate for the antifungal drug because of its good activity against Aspergillus demonstrated in the present study, as well as in other studies in the literature. Their combination in vitro with amphotericin B or ketoconazole did not present any advantages over the use of antifungal drugs alone.

scholarly journals IN VITRO ANTIFUNGAL ACTIVITY OF FLOWER EXTRACT OF PANDANUS ODORATISSIMUS AGAINST DERMATOPHYTIC FUNGI

2018 ◽  
Vol 11 (9) ◽  
pp. 325
Author(s):  
Geethavani Babu ◽  
Balamuruganvelu Singaravelu ◽  
Sreenivasalu Reddy Vallapu ◽  
Srikumar Ramasundaram
Keyword(s):  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Epidermophyton Floccosum ◽  
Dermatophytic Fungi ◽  
Pandanus Odoratissimus ◽  
In Vitro Antifungal Activity ◽  
Tube Dilution

Objective: The present study aimed to evaluate the antifungal activity of Pandanus odoratissimus oil against dermatophytic fungi, and it was compared against the two commonly used antifungal agent’s fluconazole and griseofulvin.Methods: A total of seven strains of dermatophytes were tested for antifungal activity using oil extracted from the flower of P. odoratissimus by using agar-well diffusion method and the zone of inhibition was compared with antifungal agent’s fluconazole and griseofulvin. Minimum inhibitory concentration (MIC) was determined using the tube-dilution method.Results and Conclusion: The zone of inhibition varied from 16.32 to 19.76 mm for fluconazole, 12.12–18.16 mm for griseofulvin, and 2.5–9.59 mm and 7.63–12.88 mm for 2.5 mg/ml and 5 mg/ml of P. odoratissimus extract, respectively. Epidermophyton floccosum and Trichophyton violaceum showed the lowest MIC value of 0.15 mg/ml. The results of our study have shown that the extract from P. odoratissimus can work significantly better against fungal diseases caused by dermatophytes. It was also found that it acts as a perfect alternative to the currently available antifungals such as fluconazole and griseofulvin. 

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