Molecular analysis of the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children in Rio de Janeiro, Brazil

2007 ◽  
Vol 56 (6) ◽  
pp. 854-859 ◽  
Author(s):  
Irene Trigueiros Araújo ◽  
Marcos Bryan Heinemann ◽  
Joana D'Arc P. Mascarenhas ◽  
Rosane M. Santos Assis ◽  
Alexandre Madi Fialho ◽  
...  
Keyword(s):  
Hospitalized Children ◽  
Structural Protein ◽  
Genetic Groups ◽  
Nsp4 Genotype ◽  
Group A Rotaviruses ◽  
Group A ◽  
Viral Morphogenesis ◽  
Outer Capsid ◽  
Genotype A

Group A rotaviruses are the main cause of acute gastroenteritis in children throughout the world. The two outer capsid proteins, VP4 and VP7, define the P and G genotypes, respectively. Rotaviruses with P[8]G1, P[4]G2, P[8]G3 and P[8]G4 genotypes are predominant in infecting humans and the G9 genotype is emerging in most continents as the fifth most common G type worldwide. The inner capsid protein VP6 is responsible for subgroup (SG) specificities, allowing classification of rotaviruses into SG I, SG II, SG I+II and SG non-I-non-II. The non-structural protein 4 (NSP4) encoded by segment 10 has a role in viral morphogenesis and five genetic groups have been described, NSP4 genotypes A–E. The aim of this investigation was to characterize the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children. Thirty rotavirus strains were submitted to RT-PCR followed by sequencing and phylogenetic analysis. Among the different G and P genotype combinations, two distinct genetic groups could be recognized for the NSP4 gene. Twenty-eight clustered with NSP4 genotype B. The two P[4]G2 strains fell into NSP4 genotype A and clustered distinctly, with a 100 % bootstrap value. The strains distinguished within a group were closely related to each other at the nucleotide and amino acid levels. A phylogenetic tree was constructed for the VP6 gene including the human strains RMC100, E210, Wa, US1205 and 1076, and the animal strains Gott, NCDV, SA-11, FI-14 and EW. This is the first report on Brazilian rotavirus strains describing NSP4 genotype A strains associated with VP6 SG I, and NSP4 genotype B strains associated with VP6 SG II.

scholarly journals Rotaviruses and Rotavirus Vaccines

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 959
Author(s):  
Celeste M. Donato ◽  
Julie E. Bines
Keyword(s):  
Capsid Proteins ◽  
Virus Family ◽  
Rotavirus Vaccines ◽  
Group A Rotaviruses ◽  
Group A ◽  
Outer Capsid

Group A rotaviruses belong to the Reoviridae virus family and are classified into G and P genotypes based on the outer capsid proteins VP7 and VP4, respectively [...]

scholarly journals Survey of Human Group C Rotaviruses in Japan during the Winter of 1992 to 1993

1998 ◽  
Vol 36 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Mitsutaka Kuzuya ◽  
Ritsushi Fujii ◽  
Masako Hamano ◽  
Masao Yamada ◽  
Kuniko Shinozaki ◽  
...  
Keyword(s):  
Large Scale ◽  
Blot Hybridization ◽  
Human Group ◽  
Dot Blot ◽  
Passive Hemagglutination ◽  
Group A Rotaviruses ◽  
Group A ◽  
Outer Capsid ◽  
Very High ◽  
Vp7 Gene

Fecal specimens from patients with acute diarrhea were collected from 10 prefectures in Japan over a 6-month period (November 1992 to April 1993), and the specimens that were negative for human group A rotaviruses were screened for the presence of human group C rotaviruses (CHRVs) by the reverse passive hemagglutination test. Of 784 specimens examined, 53 samples (6.8%) that were collected in 7 of 10 prefectures were positive for CHRV, indicating that CHRVs are widely distributed across Japan. Most of the CHRV isolates were detected in March and April, and CHRVs mainly prevailed in children ages 3 to 8 years. The genome electropherotypes of eight strains isolated in five individual prefectures were surprisingly similar to each other and were different from those of CHRV strains isolated to date. The outer capsid glycoprotein (VP7) gene homologies of the isolates retrieved in 1993 were subsequently analyzed by the dot blot hybridization method. As a result, the VP7 genes of the isolates revealed very high levels of homology not only with each other but also with the VP7 gene of the OK118 strain isolated in 1988. These results suggest that a large-scale outbreak of CHRV occurred during the winter of 1992 and 1993 in Japan.

Molecular analysis of group A rotaviruses detected in hospitalized children from Rawalpindi, Pakistan during 2014

2017 ◽  
Vol 53 ◽  
pp. 160-166 ◽  
Author(s):  
Massab Umair ◽  
Bilal Haider Abbasi ◽  
Nadia Nisar ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Hospitalized Children ◽  
Group A Rotaviruses ◽  
Group A

scholarly journals The epidemiology of acute viral gastroenteritis in hospitalized children in Cordoba city, Argentina: an insight of disease burden

2001 ◽  
Vol 43 (4) ◽  
pp. 193-197 ◽  
Author(s):  
Miguel O. GIORDANO ◽  
Leonardo J. FERREYRA ◽  
María B. ISA ◽  
Laura C. MARTINEZ ◽  
Silvia I. YUDOWSKY ◽  
...  
Keyword(s):  
Disease Burden ◽  
Gastrointestinal Disease ◽  
Viral Gastroenteritis ◽  
Hospitalized Children ◽  
Viral Diarrhea ◽  
Group A Rotaviruses ◽  
Group A ◽  
Immuno Assay ◽  
Enzyme Immuno Assay ◽  
The Impact

Information concerning the disease burden of viral gastroenteritis has important implications for the use and monitoring the impact of public health policies. The present study, carried out in Córdoba city, Argentina, documents the epidemiology of severe viral diarrhea as well as the burden of viral gastrointestinal disease in the hospital children admission. A total of 133 stools were collected from hospitalized children (Town Childhood Hospital) suffering from acute diarrhea and studied for the presence of Group A rotavirus, astrovirus and adenovirus 40/41 by enzyme-immuno assay, between November 1997 and October 1998. Enteric viruses accounted for 42.1% of the total diarrheal cases analyzed. Group A rotaviruses, astroviruses, adenoviruses 40/41 and mixed infections were found in 35.3, 4.5, 1.5, and 0.8% studied specimens respectively. We estimated that 1 in 27 children in the 0-35 month-old cohort/range would be annually hospitalized for a viral gastroenteritis illness. The major impact on viral diarrhea lies on rotaviral infection, accouting for 84.0% of the viral diarrheal cases analyzed and for approximately one third of severe diarrheas requiring hospital admission in Córdoba City, Argentina.

scholarly journals RotaC: A web-based tool for the complete genome classification of group A rotaviruses

2009 ◽  
Vol 9 (1) ◽  
pp. 238 ◽  
Author(s):  
Piet Maes ◽  
Jelle Matthijnssens ◽  
Mustafizur Rahman ◽  
Marc Van Ranst
Keyword(s):  
Complete Genome ◽  
Web Based ◽  
Group A Rotaviruses ◽  
Group A

scholarly journals Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments

2008 ◽  
Vol 153 (8) ◽  
pp. 1621-1629 ◽  
Author(s):  
Jelle Matthijnssens ◽  
Max Ciarlet ◽  
Mustafizur Rahman ◽  
Houssam Attoui ◽  
Krisztián Bányai ◽  
...  
Keyword(s):  
Genomic Rna ◽  
Group A Rotaviruses ◽  
Group A

scholarly journals A Survey of G6 and G10 Serotypes of Group a Bovine Rotaviruses from Diarrheic Beef and Dairy Calves using Monoclonal Antibodies in ELISA

1994 ◽  
Vol 6 (2) ◽  
pp. 175-181 ◽  
Author(s):  
A. Lucchelli ◽  
S. Y. Kang ◽  
M. K. Jayasekera ◽  
A. V. Parwani ◽  
D. H. Zeman ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
South Dakota ◽  
Dairy Herd ◽  
Dairy Calves ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Stool ◽  
Field Samples ◽  
Group A Rotaviruses ◽  
Beef Herds ◽  
Group A

Group A bovine rotaviruses (BRV) have been identified worldwide as a major cause of diarrhea in the young of many species, including humans. Group A rotaviruses are classified into serotypes on the basis of the outer capsid proteins, VP7 (G types) and VP4 (P types). To date, there are 14 G types of group A rotaviruses, with G1, G6, G8, and G10 described for BRV isolates. In this study, G6- and G lo-specific monoclonal antibodies (MAbs) were used in an enzyme-linked immunosorbent assay (ELISA) for the G typing of BRV-positive stool samples from diarrheic beef and dairy calves from South Dakota, Ohio, Michigan, Nebraska, and Washington, USA, and Ontario, Canada. ELISA plates were coated using a broadly reactive VP7 MAb (Common 60) or with G6- or G10-specific MAbs. BRV-positive fecal samples were diluted and added to duplicate wells, followed by the addition of polyclonal guinea pig anti-group A rotavirus serum as the secondary antibody. Several reference G6 and G10 BRV strains as well as other G types previously reported in cattle (G1, G2, G3, G8) and BRV-negative samples were included as G type specificity and negative controls. From a total of 308 field samples analyzed, 79% (244/308) tested positive by the broadly reactive VP7 MAb; of these, 54% (131/244) were G6 positive, 14% (35/244) were G10 positive, 4% (9/244) were both G6 and G10 positive, and 28% (69/244) were G6 and G10 negative. The negative samples may represent additional or undefined serotypes. The 89 samples from South Dakota were further subdivided into samples from beef ( n = 43) or dairy ( n = 46) herds. G6 was more prevalent in beef herd samples (67%) than in dairy herd samples (47.5%). In addition, dairy herds had higher percentages of G10-positive samples (17.5%) G6-G10 double positives (10%), and untypable samples (25%) than did beef herds, in which the prevalence of G10 positive samples was 5.5%, G6-G10 double positives was 5.5%, and untypable samples was 22%. Application of the serotype ELISA for the analysis of additional BRV samples will provide further epidemiologic data on the distribution of BRV serotypes in beef or dairy cattle, an important consideration for the development of improved BRV vaccines.

scholarly journals Expressive arts therapy for hospitalized children: a pilot study measuring cortisol levels

2013 ◽  
Vol 5 (2) ◽  
pp. 7 ◽  
Author(s):  
Garret Yount ◽  
Kenneth Rachlin ◽  
Jane Siegel
Keyword(s):  
Pilot Study ◽  
Treatment Group ◽  
Expressive Arts ◽  
Control Group ◽  
Hospitalized Children ◽  
Expressive Arts Therapy ◽  
Wait List Control Group ◽  
Wait List Control ◽  
Group A ◽  
Cortisol Levels

This pilot study aimed at assessing the feasibility of capturing physiological evidence of reduced stress for hospitalized children following expressive arts therapy. Twenty-five patients were offered a novel form of expressive arts therapy, termed Healing Sock Creatures, during their stay in the hospital. Saliva samples were collected at two times in the afternoon for the purpose of measuring salivary cortisol levels. The patients were randomly assigned to two groups, a treatment group or a wait-list control group. A trend of decreased cortisol levels was apparent following therapy in the treatment group and concurrent steroid treatment, which is common in intensive care units, does not appear to interfere with the ability to measure decreased cortisol levels following therapy. Our results support the design of a formal study to assess physiological biomarkers of stress in hospital settings. To our knowledge, this is the first in-patient study assessing a biomarker of stress following expressive arts therapy for children

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