scholarly journals Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, southern India

2007 ◽  
Vol 56 (2) ◽  
pp. 260-265 ◽  
Author(s):  
Saswat S. Mohapatra ◽  
Dhanya Ramachandran ◽  
Chinmay K. Mantri ◽  
Durg V. Singh
Keyword(s):  
Vibrio Cholerae ◽  
Rflp Analysis ◽  
Single Copy ◽  
Core Region ◽  
Southern India ◽  
The Core ◽  
Vibrio Cholerae O1 ◽  
Hybridization Profile ◽  
El Tor

Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). RFLP analysis revealed that these Inaba isolates possessed a single copy of the CTX element flanked by two tandemly arranged copies of the RS element upstream of the core region. The isolates were resistant to ampicillin, nalidixic acid, trimethoprim, sulfamethoxazole, streptomycin and the vibriostatic agent 2,4-diamino-6,7-diisopropylpteridine (O/129). Ribotyping of these Inaba isolates revealed a hybridization profile similar to a strain of serotype Ogawa prevalent in southern India.

scholarly journals Structural Characterization of the Extracellular Polysaccharide from Vibrio cholerae O1 El-Tor

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86751 ◽  
Author(s):  
Fitnat Yildiz ◽  
Jiunn Fong ◽  
Irina Sadovskaya ◽  
Thierry Grard ◽  
Evgeny Vinogradov
Keyword(s):  
Vibrio Cholerae ◽  
Structural Characterization ◽  
Extracellular Polysaccharide ◽  
Vibrio Cholerae O1 ◽  
El Tor

Molecular characterization of recent Vibrio cholerae O1, El Tor, Inaba strains isolated from hospitalized patients in Kolkata, India

2007 ◽  
Vol 55 (5) ◽  
pp. 431-438 ◽  
Author(s):  
Amit Raychoudhuri ◽  
Souvik Chatterjee ◽  
Gururaja P. Pazhani ◽  
Ranjan K. Nandy ◽  
Mihir K. Bhattacharya ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Molecular Characterization ◽  
Hospitalized Patients ◽  
Vibrio Cholerae O1 ◽  
El Tor

Molecular characterization of Vibrio cholerae O1 El Tor strains in Malaysia revealed genetically diverse variant lineages

2021 ◽  
Author(s):  
Kwai Lin Thong ◽  
Kathryn Bee Lin Tham ◽  
Soo Tein Ngoi ◽  
Shiang Chiet Tan ◽  
Wan Noraini Wan Yussof ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Molecular Characterization ◽  
Vibrio Cholerae O1 ◽  
El Tor

scholarly journals Characterization of Vibrio cholerae O1 Antigen as the Bacteriophage K139 Receptor and Identification of IS1004Insertions Aborting O1 Antigen Biosynthesis

2000 ◽  
Vol 182 (18) ◽  
pp. 5097-5104 ◽  
Author(s):  
Jutta Nesper ◽  
Dagmar Kapfhammer ◽  
Karl E. Klose ◽  
Hilde Merkert ◽  
Joachim Reidl
Keyword(s):  
Vibrio Cholerae ◽  
Binding Studies ◽  
Core Oligosaccharide ◽  
O Antigen ◽  
Mouse Small Intestine ◽  
Normal Human ◽  
Vibrio Cholerae O1 ◽  
Phage Receptor ◽  
El Tor

ABSTRACT Bacteriophage K139 was recently characterized as a temperate phage of O1 Vibrio cholerae. In this study we have determined the phage adsorption site on the bacterial cell surface. Phage-binding studies with purified lipopolysaccharide (LPS) of different O1 serotypes and biotypes revealed that the O1 antigen serves as the phage receptor. In addition, phage-resistant O1 El Tor strains were screened by using a virulent isolate of phage K139. Analysis of the LPS of such spontaneous phage-resistant mutants revealed that most of them synthesize incomplete LPS molecules, composed of either defective O1 antigen or core oligosaccharide. By applying phage-binding studies, it was possible to distinguish between receptor mutants and mutations which probably caused abortion of later steps of phage infection. Furthermore, we investigated the genetic nature of O1-negative strains by Southern hybridization with probes specific for the O antigen biosynthesis cluster (rfb region). Two of the investigated O1 antigen-negative mutants revealed insertions of element IS1004 into the rfb gene cluster. Treating onewbeW::IS1004 serum-sensitive mutant with normal human serum, we found that several survivors showed precise excision of IS1004, restoring O antigen biosynthesis and serum resistance. Investigation of clinical isolates by screening for phage resistance and performing LPS analysis of nonlysogenic strains led to the identification of a strain with decreased O1 antigen presentation. This strain had a significant reduction in its ability to colonize the mouse small intestine.

scholarly journals Comparative Genomic Characterization of a Thailand–Myanmar Isolate, MS6, of Vibrio cholerae O1 El Tor, Which Is Phylogenetically Related to a “US Gulf Coast” Clone

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e98120 ◽  
Author(s):  
Kazuhisa Okada ◽  
Mathukorn Na-Ubol ◽  
Wirongrong Natakuathung ◽  
Amonrattana Roobthaisong ◽  
Fumito Maruyama ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gulf Coast ◽  
Comparative Genomic ◽  
Genomic Characterization ◽  
Vibrio Cholerae O1 ◽  
El Tor
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