Booster vaccination with safe, modified, live-attenuated mutants of Brucella abortus strain RB51 vaccine confers protective immunity against virulent strains of B. abortus and Brucella canis in BALB/c mice

ABSTRACTThis study characterized the efficacy of theBrucella abortusstrain RB51 vaccine in bison when delivered by single intramuscular vaccination (hand RB51), by single pneumatic dart delivery (dart RB51), or as two vaccinations approximately 13 months apart (booster RB51) in comparison to control bison. All bison were challenged intraconjunctivally in midgestation with 107CFU ofB. abortusstrain 2308 (S2308). Bison were necropsied and sampled within 72 h of abortion or delivery of a live calf. Compared to nonvaccinated bison, bison in the booster RB51 treatment had a reduced (P< 0.05) incidence of abortion, uterine infection, or infection in maternal tissues other than the mammary gland at necropsy. Bison in single-vaccination treatment groups (hand RB51 and dart RB51) did not differ (P> 0.05) from the control group in the incidence of abortion or recovery of S2308 from uterine, mammary, fetal, or maternal tissues at necropsy. Compared to nonvaccinated animals, all RB51 vaccination groups had reduced (P< 0.05) mean colonization or incidence of infection in at least 2 of 4 target tissues, with the booster RB51 group having reduced (P< 0.05) colonization and incidence of infection in all target tissues. Our data suggest that booster vaccination of bison with RB51 enhances protective immunity againstBrucellachallenge compared to single vaccination with RB51 by hand or by pneumatic dart. Our study also suggests that an initial vaccination of calves followed by booster vaccination as yearlings should be an effective strategy for brucellosis control in bison.

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Brucella abortus RB51 Induces Protection in Mice Orally Infected with the Virulent Strain B. abortus 2308

Infection and Immunity ◽

10.1128/iai.71.5.2326-2330.2003 ◽

2003 ◽

Vol 71 (5) ◽

pp. 2326-2330 ◽

Cited By ~ 23

Author(s):

Paolo Pasquali ◽

Adone Rosanna ◽

Claudia Pistoia ◽

Paola Petrucci ◽

Franco Ciuchini

Keyword(s):

Immune Response ◽

Brucella Abortus ◽

Protective Immunity ◽

Virulent Strain ◽

Oral Route ◽

Oral Infection ◽

Virulent Strains ◽

Cell Mediated Immune Response ◽

Oral Inoculation ◽

Common Causes

ABSTRACT Brucellae are gram-negative, facultative intracellular bacteria which are one of the most common causes of abortion in animals. In addition, they are the source of a severe zoonosis. In this trial, we evaluated the effect of oral inoculation of Brucella abortus RB51 in mice against a challenge infection with B. abortus 2308. First, we showed that a gastric acid neutralization prior to the oral inoculation contributed to a more homogeneous and consistent infection with both vaccine strain B. abortus RB51 and virulent strain B. abortus 2308. Successively, we assessed the clearance and the immune response following an oral infection with B. abortus RB51. Oral inoculation gave a mild infection which was cleared 42 days after infection, and it induced a delayed humoral and cell-mediated immune response. Finally, we immunized mice by oral inoculation with B. abortus RB51, and we challenged them with the virulent strain B. abortus 2308 by an oral or intraperitoneal route 42 days after vaccination. Oral inoculation of B. abortus RB51 was able to give protection to mice infected with the virulent strain B. abortus 2308 by the oral route but not to mice infected intraperitoneally. Our results indicate that oral inoculation of mice with B. abortus RB51 is able to give a protective immunity against an oral infection with virulent strains, and this protection seems to rely on an immune response at the mucosal level.

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Evaluation of DNA vaccine encoding BCSP31 surface protein of Brucella abortus for protective immunity

Microbial Pathogenesis ◽

10.1016/j.micpath.2018.10.016 ◽

2018 ◽

Vol 125 ◽

pp. 514-520 ◽

Cited By ~ 1

Author(s):

Waqar Imtiaz ◽

Ahrar Khan ◽

Shafia Tehseen Gul ◽

Muhammad Saqib ◽

Muhammad Kashif Saleemi ◽

...

Keyword(s):

Dna Vaccine ◽

Brucella Abortus ◽

Protective Immunity ◽

Surface Protein

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Evaluation of Protection Afforded by Brucella abortus and Brucella melitensis Unmarked Deletion Mutants Exhibiting Different Rates of Clearance in BALB/c Mice

Infection and Immunity ◽

10.1128/iai.01787-05 ◽

2006 ◽

Vol 74 (7) ◽

pp. 4048-4057 ◽

Cited By ~ 59

Author(s):

M. M. Kahl-McDonagh ◽

T. A. Ficht

Keyword(s):

Mouse Model ◽

Brucella Abortus ◽

Protective Immunity ◽

Brucella Melitensis ◽

Deletion Mutants ◽

Heterologous Challenge ◽

Current Vaccine ◽

Protection Against Infection ◽

Ideal Vaccine

ABSTRACT Research for novel Brucella vaccines has focused upon the development of live vaccine strains, which have proven more efficacious than killed or subunit vaccines. In an effort to develop improved vaccines, signature-tagged mutant banks were screened to identify mutants attenuated for survival. Mutants selected from these screens exhibited various degrees of attenuation characterized by the rate of clearance, ranging from a failure to grow in macrophages after 24 h of infection to a failure to persist in the mouse model beyond 8 weeks. Ideal vaccine candidates should be safe to the host, while evoking protective immunity. In the present work, we constructed unmarked deletion mutants of three gene candidates, manBA, virB2, and asp24, in both Brucella abortus and Brucella melitensis. The Δasp24 mutants, which persist for extended periods in vivo, are superior to current vaccine strains and to other deletion strains tested in the mouse model against homologous challenge infection after 12, 16, and 20 weeks postvaccination. The Δasp24 mutants also display superior protection compared to ΔmanBA and ΔvirB2 mutants against heterologous challenge in mice. From this study, a direct association between protection against infection and cytokine response was not apparent between all vaccine groups and, therefore, correlates of protective immunity will need to be considered further. A distinct correlation between persistence of the vaccine strain and protection against infection was corroborated.

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Brucella abortus Δ cydC Δ cydD and Δ cydC Δ purD double-mutants are highly attenuated and confer long-term protective immunity against virulent Brucella abortus

Vaccine ◽

10.1016/j.vaccine.2015.11.030 ◽

2016 ◽

Vol 34 (2) ◽

pp. 237-244 ◽

Cited By ~ 4

Author(s):

Quang Lam Truong ◽

Youngjae Cho ◽

Soyeon Park ◽

Kiju Kim ◽

Tae-Wook Hahn

Keyword(s):

Brucella Abortus ◽

Protective Immunity

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Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil

Acta Amazonica ◽

10.1590/1809-4392201403486 ◽

2015 ◽

Vol 45 (3) ◽

pp. 265-270 ◽

Cited By ~ 3

Author(s):

Giselle Souza da PAZ ◽

Katarine de Souza ROCHA ◽

Michele de Souza LIMA ◽

Ediene Moura JORGE ◽

José Carlos Figueiredo PANTOJA ◽

...

Keyword(s):

Brucella Abortus ◽

Bacterial Infections ◽

Agar Gel ◽

Source Of Infection ◽

Brucella Canis ◽

Leptospira Spp ◽

The City

Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination with buffered acidified plate antigen (BAPAT) was used. For anti-Leptospira spp. antibodies research, the Microscopic Agglutination Technique (MAT) was used. No animal reacted to Brucella abortus and one animal was reactive to B. canis at the AGID, but it was negative to the AGID-2ME test. Seventeen percent of dogs (47/274) presented anti-Leptospira spp. antibodies, with prevalence of serovar Canicola. The dogs from Belém and Castanhal are not source of infection for B. abortus and B. canis, however, they are reservoirs for different serovars of Leptospira spp.

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Anticorpos anti-Brucella canis e anti-Brucella abortus em cães de Araguaína, Tocantins

Brazilian Journal of Veterinary Research and Animal Science ◽

10.11606/s1413-95962011000200010 ◽

2011 ◽

Vol 48 (2) ◽

pp. 167 ◽

Cited By ~ 2

Author(s):

Elaine Maria Seles Dorneles ◽

Helcileia Santos ◽

Silvia Minharro ◽

Josefa Moreira do Nascimento-Rocha ◽

Luis Antônio Mathias ◽

...

Keyword(s):

Brucella Abortus ◽

Agar Gel ◽

Brucella Canis

Os objetivos deste estudo foram determinar a soroprevalência da infecção por Brucella canis e Brucella abortus e avaliar os possíveis fatores de risco associados à infecção em cães no município de Araguaína, Tocantins. Soros de 374 cães, pertencentes à zona urbana do município de Araguaína-Tocantins, foram analisados pelas técnicas de imunodifusão em ágar gel (IDGA), para pesquisa de anticorpos contra Brucella canis, e antígeno acidificado tamponado (AAT) e polarização fluorescente (FPA) para detecção de anticorpos contra Brucella abortus. Dos 374 soros testados para presença de anticorpos contra B. abortus, 21 foram reagentes no AAT, entretanto todos foram negativos pela FPA. À prova do IDGA 167 animais foram reagentes resultando em uma prevalência para B. canis de 44,53% (IC 95%; 39,43 a 49,72). A avaliação de possíveis fatores de risco associados à soropositividade para B. canis não revelou a existência de relação entre a infecção e as variáveis individuais estudadas. Assim, o presente estudo permite concluir que não houve animais infectados por B. abortus e que a infecção por B. canis está disseminada nos cães do município de Araguaína, Tocantins.

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Brucella abortus Δ rpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis

Vaccine ◽

10.1016/j.vaccine.2016.08.076 ◽

2016 ◽

Vol 34 (42) ◽

pp. 5073-5081 ◽

Cited By ~ 5

Author(s):

Jonathan W. Willett ◽

Julien Herrou ◽

Daniel M. Czyż ◽

Jason X. Cheng ◽

Sean Crosson

Keyword(s):

Mouse Model ◽

Brucella Abortus ◽

Protective Immunity ◽

Wild Type

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NFB is differentially activated in macrophages from J774A.1 cell line infected with vaccine or virulent strains of Brucella abortus

African Journal of Microbiology Research ◽

10.5897/ajmr10.901 ◽

2011 ◽

Vol 5 (8) ◽

pp. 941-949

Author(s):

Cervantes Flores Maribel ◽

Martiacute nez Romero Aurora ◽

Guadalupe Ramiacute rez Valles Eda ◽

Saucedo Mendiola Leticia ◽

C Moreno Lafont Martha ◽

...

Keyword(s):

Cell Line ◽

Brucella Abortus ◽

Virulent Strains

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Identification and Characterization of a Brucella abortus ATP-Binding Cassette Transporter Homolog to Rhizobium meliloti ExsA and Its Role in Virulence and Protection in Mice

Infection and Immunity ◽

10.1128/iai.70.9.5036-5044.2002 ◽

2002 ◽

Vol 70 (9) ◽

pp. 5036-5044 ◽

Cited By ~ 34

Author(s):

G. M. S. Rosinha ◽

Daniela A. Freitas ◽

Anderson Miyoshi ◽

Vasco Azevedo ◽

Eleonora Campos ◽

...

Keyword(s):

Abc Transporter ◽

Abc Transporters ◽

Brucella Abortus ◽

Protective Immunity ◽

Deletion Mutant ◽

Rhizobium Meliloti ◽

Host Cells ◽

Atp Binding ◽

Ortholog Group ◽

Atp Binding Cassette

ABSTRACT Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of Brucella spp. is not fully understood yet. Furthermore, genes that allow Brucella to reach the intracellular niche and to interact with host cells need to be identified. Using the genomic survey sequence (GSS) approach, we identified the gene encoding an ATP-binding cassette (ABC) transporter of B. abortus strain S2308. The deduced amino acid sequence encoded by this gene exhibited 69 and 67% identity with the sequences of the ABC transporters encoded by the exsA genes of Rhizobium meliloti and Mesorhizobium loti, respectively. Additionally, B. abortus ExsA, like R. meliloti and M. loti ExsA, possesses ATP-binding motifs and the ABC signature domain features of a typical ABC transporter. Furthermore, ortholog group analysis placed B. abortus ExsA in ortholog group 6 of ABC transporters more likely to be involved in bacterial pathogenesis. In R. meliloti, ExsA is an exopolysaccharide transporter essential for alfalfa root nodule invasion and establishment of infection. To test the role of ExsA in Brucella pathogenesis, an exsA deletion mutant was constructed. Replacement of the wild-type exsA by recombination was demonstrated by Southern blot analysis of Brucella genomic DNA. Decreased survival in mice of the Brucella ΔexsA mutant compared to the survival of parental strain S2308 demonstrated that ExsA is critical for full bacterial virulence. Additionally, the B. abortus exsA deletion mutant was used as a live vaccine. Challenge experiments revealed that the exsA mutant strain induced superior protective immunity in BALB/c mice compared to the protective immunity induced by strain S19 or RB51.

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