Two out of the 11 genes of an unusual human G6P[6] rotavirus isolate are of bovine origin

In 2003, we described the first human G6P[6] rotavirus strain (B1711). To investigate further the molecular origin of this strain and to determine the possible reassortments leading to this new gene constellation, the complete genome of strain B1711 was sequenced. SimPlot analyses were conducted to compare strain B1711 with other known rotavirus gene segments, and phylogenetic dendrograms were constructed to analyse the origin of the eleven genome segments of strain B1711. Our analysis indicated that strain B1711 acquired its VP1-, VP2-, VP4-, VP6- and NSP1–5-encoding gene segments from human DS-1-like P[6] rotavirus strains, and its VP3 and VP7 gene segments from a bovine rotavirus strain through reassortment. The introduction of animal–human reassortant strains, which might arise in either of the hosts, into the human rotavirus population is an important mechanism for the generation of rotavirus diversity, and might be a challenge for the current rotavirus vaccines and vaccines under development.

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Protection Studies in Colostrum-Deprived Piglets of a Bovine Rotavirus Vaccine Candidate Using Human Rotavirus Strains for Challenge

The Journal of Infectious Diseases ◽

10.1093/infdis/148.6.1061 ◽

1983 ◽

Vol 148 (6) ◽

pp. 1061-1068 ◽

Cited By ~ 34

Author(s):

G. Zissis ◽

J. P. Lambert ◽

P. Marbebant ◽

D. Marissens ◽

M. Lobmann ◽

...

Keyword(s):

Vaccine Candidate ◽

Rotavirus Vaccine ◽

Bovine Rotavirus ◽

Human Rotavirus

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Detection and Differentiation of Bovine Group A Rotavirus Serotypes using Polymerase Chain Reaction-Generated Probes to the VP7 Gene

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063879200400206 ◽

1992 ◽

Vol 4 (2) ◽

pp. 148-158 ◽

Cited By ~ 12

Author(s):

Anil V. Parwani ◽

Blair I. Rosen ◽

Jorge Flores ◽

Malcolm A. McCrae ◽

Mario Gorziglia ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Blot Hybridization ◽

Northern Blot Hybridization ◽

Chain Reaction ◽

Diarrhea Virus ◽

Bovine Rotavirus ◽

Group A ◽

Polymerase Chain ◽

Vp7 Gene ◽

Hybridization Assays

Dot and Northern blot hybridization assays were developed to detect and differentiate group A bovine rotavirus serotypes using radiolabeled serotype 6 (Nebraska calf diarrhea virus [NCDV] and United Kingdom [UK] strains) or serotype 10 (Cracker [Cr] strain) VP7 gene probes. Partial length VP7-specific cDNA encompassing areas of major sequence diversity were generated by the polymerase chain reaction (PCR) using either cloned VP7 genes (NCDV and UK strains) or reverse transcribed mRNA (Cr strain) as templates. Radiolabeled probes prepared from the PCR-generated cDNA were tested at various stringency conditions to optimize the hybridization assays. At high stringency conditions (52 C, 50% formamide, 5 x standard saline citrate), the NCDV, UK, and Cr probes serotypically differentiated bovine rotavirus isolates in RNA samples prepared from cell culture-propagated viruses or in fecal specimens from infected gnotobiotic calves. The sensitivity and specificity of NCDV and Cr VP7 probes were characterized in dot blot hybridization assays, and the probes were estimated to detect at least 1 ng of viral RNA. The serotyping results obtained using VP7 probes were similar to those obtained using serologic assays. Further development of these assays may provide a useful means for the rapid detection and differentiation of bovine rotavirus serotypes in fecal samples from calves in the field.

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Determination of Bovine Rotavirus G and P Serotypes in Italy by PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.37.12.3879-3882.1999 ◽

1999 ◽

Vol 37 (12) ◽

pp. 3879-3882 ◽

Cited By ~ 37

Author(s):

E. Falcone ◽

M. Tarantino ◽

L. Di Trani ◽

P. Cordioli ◽

A. Lavazza ◽

...

Keyword(s):

Reverse Transcription ◽

Clinical Signs ◽

Specific Primers ◽

Bovine Rotavirus ◽

Reverse Transcription Pcr ◽

Rotavirus Vaccines ◽

Intestinal Contents ◽

Group A

Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55.7% of the samples; viruses of the G10 and G8 serotypes were found in 34.9 and 4.7% of the samples, respectively. The virus in one sample (0.7%) was not classified due to concomitant infection with G6 and G8 strains, whereas viruses in six samples (4.0%) could not be characterized with any of the three G serotype-specific primers selected for the present study. When examined for their P-serotype specificities, viruses in 55 and 42.3% of the samples were characterized as P[11] and P[5], respectively, no P[1] serotype was identified, and viruses in 2.7% of the samples could not be classified due to multiple reactivity with both P[5]- and P[11]-specific primers. Various combinations of G and P serotypes were observed, the most frequent being G6,P[5] (38.3%), G10,P[11] (31.5%), and G6,P[11] (15.4%). The results of the present study, while contributing to a better understanding of the epidemiology of bovine rotaviruses in Italy, address the relevance of serotype specificity with regard to the constancy of the quality of bovine rotavirus vaccines under different field conditions.

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Genome Sequence of an Unusual Human G10P[8] Rotavirus Detected in Vietnam

Journal of Virology ◽

10.1128/jvi.01588-12 ◽

2012 ◽

Vol 86 (18) ◽

pp. 10236-10237 ◽

Cited By ~ 12

Author(s):

Yuki Matsushima ◽

Etsuko Nakajima ◽

Tuan Anh Nguyen ◽

Hideaki Shimizu ◽

Atsuko Kano ◽

...

Keyword(s):

Genetic Analysis ◽

Genome Sequence ◽

Acute Gastroenteritis ◽

Immune Escape ◽

Coding Sequences ◽

Bovine Rotavirus ◽

Human Rotavirus ◽

Human Viruses

A rare human G10P[8] rotavirus with a reassortment between bovine and human viruses was detected from a patient with acute gastroenteritis in Vietnam. Genetic analysis using complete coding sequences of all segments showed a genomic constellation of this virus of G10-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Its VP7 region was genetically related to that of a bovine rotavirus derived from Australia (strain VICG10.01), whereas all other genes were identical to those of a human rotavirus derived from Australia (strain Victoria/CK00047). These results indicate a possibility that the reassortment of the rotavirus was caused by immune escape in Australia and the rotavirus was carried to Vietnam. Additionally, this finding will help further understanding the evolution of rotaviruses circulating in Vietnam.

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Human rotavirus vaccines: too early for the strain to tell

The Lancet ◽

10.1016/s0140-6736(08)60501-2 ◽

2008 ◽

Vol 371 (9619) ◽

pp. 1144-1145 ◽

Cited By ~ 30

Author(s):

Keith Grimwood ◽

Carl D Kirkwood

Keyword(s):

Rotavirus Vaccines ◽

Human Rotavirus

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Complete genome sequence analysis of candidate human rotavirus vaccine strains RV3 and 116E

Virology ◽

10.1016/j.virol.2010.06.005 ◽

2010 ◽

Vol 405 (1) ◽

pp. 201-213 ◽

Cited By ~ 31

Author(s):

Christine M. Rippinger ◽

John T. Patton ◽

Sarah M. McDonald

Keyword(s):

Sequence Analysis ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Rotavirus Vaccine ◽

Genome Sequence Analysis ◽

Human Rotavirus ◽

Complete Genome Sequence Analysis

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Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61 A) with different serotype specificity from serotype 6

Nucleic Acids Research ◽

10.1093/nar/18.15.4613 ◽

1990 ◽

Vol 18 (15) ◽

pp. 4613-4613 ◽

Cited By ~ 5

Author(s):

K. Taniguchi ◽

Y. Pongsuwanna ◽

M. Choonthanom ◽

S. Urasawa

Keyword(s):

Nucleotide Sequence ◽

Bovine Rotavirus ◽

Vp7 Gene

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Whole-genome analysis reveals the complex evolutionary dynamics of Kenyan G2P[4] human rotavirus strains

Journal of General Virology ◽

10.1099/vir.0.033001-0 ◽

2011 ◽

Vol 92 (9) ◽

pp. 2201-2208 ◽

Cited By ~ 22

Author(s):

Souvik Ghosh ◽

Noriaki Adachi ◽

Zipporah Gatheru ◽

James Nyangao ◽

Dai Yamamoto ◽

...

Keyword(s):

Genome Analysis ◽

Complete Genome ◽

Evolutionary Dynamics ◽

Genomic Analysis ◽

Whole Genome ◽

Genome Sequences ◽

Whole Genome Analysis ◽

Human Rotavirus ◽

Genotype Constellation ◽

Common Causes

Although G2P[4] rotaviruses are common causes of acute childhood diarrhoea in Africa, to date there are no reports on whole genomic analysis of African G2P[4] strains. In this study, the nearly complete genome sequences of two Kenyan G2P[4] strains, AK26 and D205, detected in 1982 and 1989, respectively, were analysed. Strain D205 exhibited a DS-1-like genotype constellation, whilst strain AK26 appeared to be an intergenogroup reassortant with a Wa-like NSP2 genotype on the DS-1-like genotype constellation. The VP2-4, VP6-7, NSP1, NSP3 and NSP5 genes of strain AK26 and the VP2, VP4, VP7 and NSP1–5 genes of strain D205 were closely related to those of the prototype or other human G2P[4] strains. In contrast, their remaining genes were distantly related, and, except for NSP2 of AK26, appeared to originate from or share a common origin with rotavirus genes of artiodactyl (ruminant and camelid) origin. These observations highlight the complex evolutionary dynamics of African G2P[4] rotaviruses.

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