scholarly journals Increasing seroprevalence of Human herpesvirus 8 (HHV-8) with age confirms HHV-8 endemicity in Amazon Amerindians from Brazil

2005 ◽  
Vol 86 (9) ◽  
pp. 2433-2437 ◽  
Author(s):  
A. M. G. Cunha ◽  
A. Caterino-de-Araujo ◽  
S. C. B. Costa ◽  
E. Santos-Fortuna ◽  
N. C. A. Boa-Sorte ◽  
...  
Keyword(s):  
Familial Aggregation ◽  
Horizontal Transmission ◽  
Age Groups ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Serum Samples ◽  
Isolated Populations ◽  
Herpesvirus 8 ◽  
High Prevalence ◽  
Amerindian Tribes

Human herpesvirus 8 (HHV-8) seroprevalences were determined in two isolated Amazon Amerindian tribes, according to age, gender and familial aggregation. Plasma and serum samples obtained from 982 Amazon Amerindians (664 Tiriyó and 318 Waiampi) were tested for antibodies against lytic and latent HHV-8 antigens by using ‘in-house’ immunofluorescence assays. Overall, HHV-8 seroprevalence was 56·8 % (57·4 % in the Tiriyó tribe and 55·7 % in the Waiampi tribe). Seroprevalence was independent of gender and increased linearly with age: it was 35·0 % among children aged 2–9 years, 51·4 % in adolescents (10–19 years), 72·9 % in adults and 82·3 % in adults aged >50 years. Interestingly, 44·4 % of children under 2 years of age were HHV-8-seropositive. No significant differences in seroprevalence between tribes and age groups were detected. It is concluded that HHV-8 is hyperendemic in Brazilian Amazon Amerindians, with vertical and horizontal transmission during childhood, familial transmission and sexual contact in adulthood contributing to this high prevalence in these isolated populations.

scholarly journals Prevalence of human herpesvirus 8 antibodies in the population of Belém, Pará, Brazil

2002 ◽  
Vol 44 (6) ◽  
pp. 309-313 ◽  
Author(s):  
Ronaldo B. FREITAS ◽  
Maria Rute FREITAS ◽  
Alexandre C. LINHARES
Keyword(s):  
Geometric Mean ◽  
Age Groups ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Herpesvirus 8 ◽  
North Brazil ◽  
Significant Difference ◽  
Male Subjects

Serum samples from 497 children and adults inhabiting two neighbourhoods (Guamá and Terra Firme) in Belém, Pará, North Brazil were screened for the presence of human herpesvirus 8 (HHV-8) antibody using an enzyme-linked immunosorbent assay. An overall 16.3% prevalence was found for these urban communities. Taken both genders together, prevalence rates of HHV-8 antibody increase gradually, across age-groups, ranging from 12.0% to 33.3%. When seroprevalence is analysed by gender, similar rates are found for female (18.4%) and male (14.0%) individuals. In the former gender group, seroprevalence rates increased from 10.3%, in children <FONT FACE=Symbol>£</FONT> 10 years of age, to 30.0% in adults 41-50 years of age. Conversely, among male subjects, the prevalence of HHV-8 antibodies decreased from 13.3% in children/young adults aged <FONT FACE=Symbol>£</FONT> 10 to 20 years of age to 6.1% in adults aged 21-30 years. From the 31-40 year-old group male onwards, seropositivity rates increased gradually, ranging from 8.3% to 66.7%. A significant difference in seropositivity rates was noted when comparing 21-30 age groups for female and male subjects: 23.3% and 6.1%, respectively (P = 0.03). Geometric mean optical densities were found to increase slightly from the lower to the higher age-groups. Our data suggest that transmission of HHV-8 occurs frequently in the general urban population of Belém, and that prevalence of antibody seems to increase with age.

scholarly journals Searching for Human Herpesvirus 8 Antibodies in Serum Samples from Patients Infected with Human Immunodeficiency Virus Type 1 and Blood Donors from São Paulo, Brazil

1999 ◽  
Vol 179 (6) ◽  
pp. 1591-1592 ◽  
Author(s):  
Adele Caterino‐de‐Araujo ◽  
Maria Luisa Calabrò ◽  
Elizabeth de los Santos‐Fortuna ◽  
Jamal Suleiman ◽  
Luigi Chieco‐Bianchi
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Serum Samples ◽  
Herpesvirus 8 ◽  
Immunodeficiency Virus

scholarly journals Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Gervillien Arnold Malonga ◽  
Aude Jary ◽  
Valentin Leducq ◽  
Dimitry Moudiongui Mboungou Malanda ◽  
Anicet Luc Magloire Boumba ◽  
...  
Keyword(s):  
Whole Blood ◽  
Highly Active Antiretroviral Therapy ◽  
Horizontal Transmission ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Nuclear Antigen ◽  
People Living With Hiv ◽  
Subtype C ◽  
Herpesvirus 8 ◽  
Living With Hiv

AbstractHuman herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nuclear antigen were detected by indirect immunofluorescence. In HHV-8 positive patients, we performed HHV-8 quantification in blood and saliva by real-time PCR and typing by Sanger sequencing of K1 open reading frame. HHV-8 seroprevalence was 19%, being male (odd ratio [OR] = 1.741, [95% Confidence interval {CI}, 0.97–3.07]; p = 0.0581) and having multiple sex partners before HIV diagnosis (OR = 1.682, [CI 95%, 0.97–2.92]; p = 0.0629) tended to be associated with HHV-8 seropositivity. Of the 64 HHV-8 seropositive patients, HHV-8 DNA was detected in 10 (16%) in saliva, 6 (9%) in whole-blood and in 2 (3%) in both whole-blood and saliva. Three out of 6 HHV-8 strains were subtypes A5, 2 subtype B1 and 1 subtype C. HHV-8 seroprevalence was relatively low with more frequent carriage in men, associated with asymptomatic oral excretion and a predominance of subtype A5. These data tend to support the hypothesis of horizontal transmission in people living with HIV in Brazzaville.

High prevalence in Central Africa of blood donors who are potentially infectious for human herpesvirus 8

Transfusion ◽  
1998 ◽  
Vol 38 (8) ◽  
pp. 771-775 ◽  
Author(s):  
L Belec ◽  
N Cancre ◽  
MC Hallouin ◽  
J Morvan ◽  
A Mohamed ◽  
...  
Keyword(s):  
Blood Donors ◽  
Central Africa ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
High Prevalence

scholarly journals Molecular characterization of strains of Human herpesvirus 8 from Japan, Argentina and Kuwait

2001 ◽  
Vol 82 (3) ◽  
pp. 499-506 ◽  
Author(s):  
Yuan-Xiang Meng ◽  
Tetsutaro Sata ◽  
Felicia R. Stamey ◽  
Alexander Voevodin ◽  
Harutaka Katano ◽  
...  
Keyword(s):  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Japanese Island ◽  
Isolated Populations ◽  
Gene Encoding ◽  
Herpesvirus 8 ◽  
Immunodeficiency Virus ◽  
Variable Gene ◽  
The Right

Current genotyping systems for Human herpesvirus 8 (HHV-8) are based on the highly variable gene encoding the K1 glycoprotein. Most strains collected worldwide cluster into two subtypes (I/A and II/C). Sequenced African strains have belonged to subtypes I/A and IV/B. Members of all three of these subtypes can have either the M or P allele at the right-hand side (RHS) of the genome. Strains obtained predominantly from aboriginal or relatively isolated populations have formed clades that branch at a distance from subtypes I/A and II/C, all being of the RHS P allele. The characterization is reported here of 16 Japanese, two Kuwaiti and five Argentine HHV-8 strains obtained from human immunodeficiency virus-infected and non-infected patients with Kaposi’s sarcoma (KS), primary effusion lymphoma, multicentric Castleman’s disease or renal transplants. K1 sequences of five Japanese, one Kuwaiti and two Argentine strains were identified as subtype I/A and eight Japanese, one Kuwaiti and three Argentine strains were subtype II/C. Three strains from elderly classic KS patients originally from Hokkaido, a northern Japanese island, were relatively closely related to strains of subtypes III/D and E. Consistent with previous observations, both the M and P alleles were identified at the RHS of subgroup I/A and II/C genomes; only the P allele was detected among the three Hokkaido strains. Distances among the Hokkaido strains were similar to the distance between subtypes I/A and II/C, suggesting that the Hokkaido strains may represent two distinct subtypes and that, as more strains are analysed, the currently recognized III/D subgroups will probably emerge as independent subtypes.

High prevalence of Human Herpesvirus 8 in schizophrenic patients

2014 ◽  
Vol 216 (2) ◽  
pp. 192-197 ◽  
Author(s):  
Neila Hannachi ◽  
Yousri El Kissi ◽  
Samar Samoud ◽  
Jaafar Nakhli ◽  
Leila Letaief ◽  
...  
Keyword(s):  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
High Prevalence ◽  
Schizophrenic Patients

scholarly journals Development of Whole-Virus Multiplex Luminex-Based Serological Assays for Diagnosis of Infections with Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Homologs in Macaques

2013 ◽  
Vol 20 (3) ◽  
pp. 409-419 ◽  
Author(s):  
Jonathan T. Ryan ◽  
Timothy M. Rose
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Large Set ◽  
Primate Research ◽  
Serum Samples ◽  
Herpesvirus 8 ◽  
Kaposi's Sarcoma Associated Herpesvirus ◽  
Kaposi’S Sarcoma Associated Herpesvirus

ABSTRACTKaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 is a tumorigenic rhadinovirus that is associated with all forms of Kaposi's sarcoma. Current serological detection of KSHV is based on enzyme-linked immunosorbent or immunofluorescence assays that suffer from a variety of problems, including the lack of defined standards for test comparison. While KSHV is the only known human rhadinovirus, two lineages of KSHV-like rhadinoviruses are found in Old World primates: the RV1 lineage includes KSHV and retroperitoneal fibromatosis herpesvirus (RFHV) in macaques, and the RV2 lineage includes RRV and MneRV2 from different macaque species. To develop animal models of KSHV-associated diseases, we developed quantitative multiplex bead-based serological assays to detect antibodies against rhadinovirus antigens. Proteins from KSHV (RV1) and MneRV2 (RV2) virions were coupled to spectrally distinct fluorescent beads and used in Luminex flow cytometry-based assays to detect immune responses in macaques. Both assays showed large dynamic ranges with high levels of seroreactivity to both KSHV and MneRV2 proteins. A large set of macaque serum samples from the Washington National Primate Research Center was screened, and most of the samples (82%) were positive in both assays, consistent with the high level of RV1-RV2 coinfection detected by PCR. The macaque sera showed broad, variable, and unique serological responses to the different viral antigens, allowing an initial seroprevalence to be determined for the macaque viruses. The Luminex assays offer a novel multiplexed approach to assess rhadinovirus infection patterns in both humans and nonhuman primates. This will help advance our understanding of rhadinovirus biology and associated host immunological responses.

High prevalence of human herpesvirus 8 (HHV-8) infection in south Texas children

2002 ◽  
Vol 67 (4) ◽  
pp. 542-548 ◽  
Author(s):  
Jacques Baillargeon ◽  
Charles T. Leach ◽  
Jian-Hong Deng ◽  
Shou-Jiang Gao ◽  
Hal B. Jenson
Keyword(s):  
Human Herpesvirus ◽  
South Texas ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
High Prevalence

scholarly journals Detection of antibodies to human herpesvirus 8 in Italian children: evidence for horizontal transmission

2000 ◽  
Vol 82 (3) ◽  
pp. 702-704 ◽  
Author(s):  
D Whitby ◽  
M Luppi ◽  
C Sabin ◽  
P Barozzi ◽  
A R Di Biase ◽  
...  
Keyword(s):  
Horizontal Transmission ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Italian Children

High prevalence of human herpesvirus 8 in a Tunisian sample of Schizophrenic patients

2011 ◽  
Vol 26 (S2) ◽  
pp. 1375-1375 ◽  
Author(s):  
Y. El Kissi ◽  
N. Hannach ◽  
S. Gaabout ◽  
S. Samoud ◽  
M. Ayachi ◽  
...  
Keyword(s):  
Human Herpesvirus ◽  
Severity Of Illness ◽  
Immunofluorescence Assay ◽  
Human Herpesvirus 8 ◽  
Professional Activity ◽  
Healthy Controls ◽  
Herpesvirus 8 ◽  
Number Of Children ◽  
High Prevalence ◽  
Schizophrenic Patients

BackgroundThe prevalence of Human Herpesvirus 8 (HHV8) has never been investigated in schizophrenic patients.ObjectiveThis study aimed to assess the prevalence of HHV8 serum antibodies in schizophrenic patients and in healthy controls.MethodsDuring a 24 months period, we consecutively enrolled 108 patients meeting DSM-IV criteria of schizophrenia, in psychiatry department of Sousse Farhat Hached hospital (Tunisia). We also enrolled 108 controls among consenting blood donors. They were age and sex matched and free from any psychotic disorder as screened by MINI-Plus.Psychopathology and severity were measured using PANSS, BPRS, SANS, SAPS and CGI. Sera samples were obtained from patients and controls and then analyzed for the presence of anti-HHV8 antibodies (anti-HHV8) using a sensitive indirect immunofluorescence assay to latent and lytic HHV8 antigens.ResultsA significantly higher prevalence of anti-HHV8 in schizophrenic patients than in healthy controls was found (28.7% vs. 14.8%, p = 0.01). Marital status, educational level, professional activity, poverty, promiscuity, number of children, sexual behavior or presence of risk factors of blood transmission were not associated with HHV8 prevalence (p > 0.05). However, among schizophrenic patients, HHV8 prevalence was statically associated with positive symptoms (SAPS score) (p = 0.01) and the severity of illness (CGI score) (p = 0.02).ConclusionTo our knowledge, this would be the first report of high HHV8 prevalence in schizophrenic patients, which support the role of this virus in the pathogenesis of schizophrenia. To go on further with this hypothesis, more investigations of HHV8 in schizophrenia are needed.

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