scholarly journals Pulse of α-2-macroglobulin and lipocalin-1 in the pregnant uterus of European polecats (Mustela putorius) at the time of implantation

2016 ◽  
Author(s):  
Heli Lindeberg ◽  
Richard J.S. Burchmore ◽  
Malcolm W. Kennedy
Keyword(s):  
Protein Profile ◽  
Antimicrobial Activities ◽  
Biochemical Properties ◽  
Cellular Damage ◽  
Secretory Proteins ◽  
Mustela Putorius ◽  
Human Form ◽  
Pregnant Uterus ◽  
Proteomics Approach ◽  
Tear Lipocalin

AbstractUterine secretory proteins protect the uterus and conceptuses against infection, facilitate implantation, control cellular damage resulting from implantation, and supply embryos with nutrients. The early conceptus of the European polecat (Mustela putorius) grows and develops free in the uterus until implanting at about 12 days after mating. Using a proteomics approach we found that the proteins appearing in the uterus leading up to and including the time of implantation changed dramatically with time. Several of the proteins identified have been found in pregnant uteri of other placental mammals, such as α1-antitrypsin, serum albumin, lactoferrin, cathepsin L1, uteroferrin, and ectonucleotide pyrophosphatase. The broad-spectrum proteinase inhibitor α2-macroglobulin rose from relatively low abundance initially to dominate the protein profile by the time of implantation. Its functions may be to limit damage caused by the release of proteinases during implantation, and to control other processes around the site of implantation. Lipocalin-1 (also known as tear lipocalin) has not previously been recorded as a uterine secretion in pregnancy, and also increased substantially in concentration. If polecat lipocalin-1 has similar biochemical properties to the human form, then it may have a combined function in transporting or scavenging lipids, and antimicrobial activities. The changes in the uterine secretory proteome of Euroepan polecats may be similar in those species of mustelid that engage in embryonic diapause, but possibly only following reactivation of the embryo.

scholarly journals Pulse of inflammatory proteins in the pregnant uterus of European polecats ( Mustela putorius ) leading to the time of implantation

2017 ◽  
Vol 4 (3) ◽  
pp. 161085 ◽  
Author(s):  
Heli Lindeberg ◽  
Richard J. S. Burchmore ◽  
Malcolm W. Kennedy
Keyword(s):  
Protein Profile ◽  
Secretory Protein ◽  
Biological Properties ◽  
Cellular Damage ◽  
Secretory Proteins ◽  
Mustela Putorius ◽  
Antimicrobial Protection ◽  
Inflammatory Proteins ◽  
Uterine Secretions ◽  
Tear Lipocalin

Uterine secretory proteins protect the uterus and conceptuses against infection, facilitate implantation, control cellular damage resulting from implantation, and supply pre-implantation embryos with nutrients. Unlike in humans, the early conceptus of the European polecat ( Mustela putorius ; ferret) grows and develops free in the uterus until implanting at about 12 days after mating. We found that the proteins appearing in polecat uteri changed dramatically with time leading to implantation. Several of these proteins have also been found in pregnant uteri of other eutherian mammals. However, we found a combination of two increasingly abundant proteins that have not been recorded before in pre-placentation uteri. First, the broad-spectrum proteinase inhibitor α 2 -macroglobulin rose to dominate the protein profile by the time of implantation. Its functions may be to limit damage caused by the release of proteinases during implantation or infection, and to control other processes around sites of implantation. Second, lipocalin-1 (also known as tear lipocalin) also increased substantially in concentration. This protein has not previously been recorded as a uterine secretion in pregnancy in any species. If polecat lipocalin-1 has similar biological properties to that of humans, then it may have a combined function in antimicrobial protection and transporting or scavenging lipids. The changes in the uterine secretory protein repertoire of European polecats is therefore unusual, and may be representative of pre-placentation supportive uterine secretions in mustelids (otters, weasels, badgers, mink, wolverines) in general.

scholarly journals Dietary Germinated Paddy Rice and Stocking Density Affect Egg Performance, Serum Biochemical Properties, and Proteomic and Transcriptomic Response of Laying Hens Exposed to Chronic Heat Stress

Proteomes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 48
Author(s):  
Tossaporn Incharoen ◽  
Sittiruk Roytrakul ◽  
Wirot Likittrakulwong
Keyword(s):  
Heat Stress ◽  
Egg Production ◽  
Laying Hens ◽  
Protein Profile ◽  
Stocking Density ◽  
Biochemical Properties ◽  
Paddy Rice ◽  
Egg Mass ◽  
Serum Samples ◽  
Transcriptomic Response

Germinated paddy rice (GPR) could be a good alternative feed source for poultry with stocking density and heat stress problems. A total of 72 Hy-line Brown laying hens raised under low (LSD, 0.12 m2/bird) and high stocking densities (HSD, 0.06 m2/bird) were investigated. Three dietary GPR levels (0, 74 and 148 g/kg) were used. It was found that average daily feed intake, hen-day egg production, and egg mass significantly decreased in the HSD group. The levels of serum glucose (GLU), phosphorous (P), corticosterone (CORT), total Ig, lysozyme (LZY), and superoxide dismutase activities (SOD) in the HSD group were higher than those in the LSD group. Dietary GPR significantly affected GLU, P, alternative complement haemolytic 50 (ACH50), total Ig, and LZY. Moreover, CORT level significantly decreased in 74 and 148 g/kg dietary GPR groups, whereas SOD significantly increased only in the 148 g/kg dietary GPR group. Serum samples were analyzed using liquid chromatography-tandem mass spectrometry, and 8607 proteins were identified. Proteome analysis revealed 19 proteins which were enriched in different stocking densities and dietary GPR levels. Quantitative real-time reverse transcription-PCR technique was successfully used to verify the differentiated abundant protein profile changes. The proteins identified in this study could serve as appropriate biomarkers.

scholarly journals Insights into the physiology of Methylotenera mobilis as revealed by metagenome-based shotgun proteomic analysis

Microbiology ◽  
2009 ◽  
Vol 155 (4) ◽  
pp. 1103-1110 ◽  
Author(s):  
Gundula Bosch ◽  
Tiansong Wang ◽  
Ekaterina Latypova ◽  
Marina G. Kalyuzhnaya ◽  
Murray Hackett ◽  
...  
Keyword(s):  
Large Scale ◽  
Protein Profile ◽  
Shotgun Proteomics ◽  
Metagenomic Sequencing ◽  
Sequence Coverage ◽  
Metagenomic Sequence ◽  
The Family ◽  
Proteomics Approach ◽  
Sequence Quality ◽  
Environmental Microbes

While the shotgun proteomics approach is gaining momentum in understanding microbial physiology, it remains limited by the paucity of high-quality genomic data, especially when it comes to poorly characterized newly identified phyla. At the same time, large-scale metagenomic sequencing projects produce datasets representing genomes of a variety of environmental microbes, although with lower sequence coverage and sequence quality. In this work we tested the utility of a metagenomic dataset enriched in sequences of environmental strains of Methylotenera mobilis, to assess the protein profile of a laboratory-cultivated strain, M. mobilis JLW8, as a proof of principle. We demonstrate that a large portion of the proteome predicted from the metagenomic sequence (approx. 20 %) could be identified with high confidence (three or more peptide sequences), thus gaining insights into the physiology of this bacterium, which represents a new genus within the family Methylophilaceae.

scholarly journals Proteomics approach to investigate dynamic protein profile involved in high fat diet-induced fatty liver disease in rats

2019 ◽  
Vol 32 (4) ◽  
pp. 223-232
Author(s):  
Baohua Huang ◽  
Yanling Yao ◽  
Yaping Li ◽  
Hua Yang ◽  
Huchen Liu ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
High Fat Diet ◽  
Fatty Liver Disease ◽  
Protein Profile ◽  
High Fat ◽  
Proteomics Approach

Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory–secretory proteins and their biochemical properties

2014 ◽  
Vol 113 (8) ◽  
pp. 2765-2776 ◽  
Author(s):  
Jinyoung Lee ◽  
Jong-Hyun Kim ◽  
Hae-Jin Sohn ◽  
Hee-Jong Yang ◽  
Byoung-Kuk Na ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Cathepsin B ◽  
Biochemical Properties ◽  
Secretory Proteins ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri

scholarly journals Profiles of circulating histidine-rich glycoprotein associate with chronological age and risk of all-cause mortality

2018 ◽  
Author(s):  
Mun-Gwan Hong ◽  
Tea Dodig-Crnković ◽  
Xu Chen ◽  
Kimi Drobin ◽  
Woojoo Lee ◽  
...  
Keyword(s):  
Meta Analysis ◽  
Protein Profile ◽  
Physiological Processes ◽  
Risk Of Mortality ◽  
Proteomics Approach ◽  
All Cause Mortality ◽  
Associated Proteins ◽  
Antibody Validation ◽  
Affinity Proteomics

1.SUMMARYDespite recognizing aging as risk factor of human diseases, little is still known about the molecular traits of biological age and mortality risk. To identify age-associated proteins circulating human blood, we screened 156 subjects aged 50-92 years using an exploratory and multiplexed affinity proteomics approach. We corroborated the top age-associated protein profile (adjusted P < 0.001) in eight additional study sets (N = 4,044 individuals), and confirmed a consistent age-associated increase (P = 6.61 × 10-6) by meta-analysis. Applying antibody validation determined circulating histidine-rich glycoprotein (HRG) as the target, and we observed that sequence variants influenced the antibodies ability to bind to the protein. Profiles of circulating HRG were associated to several clinical traits and predicted the risk of mortality during a follow-up period of 8.5 years (IQR = 7.7-9.3 years) after blood sampling (HR = 1.25 per SD; 95% CI = 1.12-1.39; P = 7.41 × 10-5). In conclusion, our affinity proteomics analysis found associations between the molecular traits of circulating HRG with age and all-cause mortality. This suggests that the profiles of multi-purpose protein HRG could serve as an accessible indicator of physiological processes related to aging.

scholarly journals Antifungal effects of 3-(4-Phenyl-thiazol-2-yl)-2-thioxo-2, 3-dihydro-1H-quinazolin-4-one against Aspergillus Species

2018 ◽  
Author(s):  
Desh D. Singh ◽  
V. K. Tiwari ◽  
Rambir Singh ◽  
G L Sharam ◽  
Rajesh Dabur
Keyword(s):  
Antifungal Activity ◽  
Protein Profile ◽  
Antifungal Drugs ◽  
Secretory Proteins ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Dilution Assay ◽  
Number Of Patients ◽  
Diffusion Assay ◽  
Microbroth Dilution

AbstractAspergillus infections have become an important health problem with the increasing number of patients. Available antifungal drugs are lack with their spectrum, toxic or immunosuppressive in nature, so that need to develop new compound with high efficacy. To evaluate antifungal efficacy of synthesized compound and to identify the protein profile of Aspergillus fumigatus treated with antifungal. Clinical isolates of A. fumigatus, A. flavus and A. niger were cultured and efficacy of compound were conducted by Disc Diffusion Assay (DDA), Microbroth Dilution Assay (MDA). Percent of spore germination inhibition assay (PSGI), Time kill analysis and toxicity assay. The culture filtrate containing secretory proteins was collected after 24 h growth and expression of downregulated proteins were identified. We developed a new and useful quinazoline derivatives expected to antifungal activity. The result of anti-Aspergillus evolution revealed that one of the 3-(4-Phenyl-thiazol-2-yl)-2-thioxo-2, 3-dihydro-1H-quinazolin-4-one (DDVKT4Q) exhibited appreciable activity. The potency of compound was found concentration of 3.125 µg/disc by disc diffusion assay (DDA) and 15.625 µg/ml. by Microbroth Dilution Assay (MDA). The compound was nontoxic up to concentration 625 µg/ml and its lysed only 35.9% of human erythrocytes, at the highest dose tested. It’s observed that the treatment of pathogen with DDVKT-4Q targeted the expression of four proteins having molecular weights 18 kDa 37 KDa and 43 KDa proteins was completely inhibited or down regulated by the compound the extra cellular. The novel compound DDVKT-4Q, having antifungal activity Can be exploited further to develop new ideal antimycotic drugs.

Biochemical Investigations on Vigour Enhancement in Fresh and Aged Seeds Upon Seed Priming in Cowpea [Vigna unguiculata (L.) Walp.]

2021 ◽  
Author(s):  
M.N. Arun ◽  
K. Bhanuprakash ◽  
S. Shankara Hebbar ◽  
T. Senthivel ◽  
A.K. Nair ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Seed Quality ◽  
Protein Profile ◽  
Seed Priming ◽  
Ammonium Molybdate ◽  
Cellular Damage ◽  
Repair System ◽  
Seed Vigour ◽  
Seed Deterioration ◽  
Aged Seeds

Background: Loss in seed quality that occurs from maturity in the field to storage, leads to seed deterioration. Storage of cowpea seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzyme and protein levels. Method: The present study was performed to verify the effects of cowpea [Vigna unguiculata (L.) Walp.] seed priming (GA3, ammonium molybdate, Ca Cl2, KBr, Mg (NO3)2, ZnSO4, Hydro priming and dry non primed) with fresh and accelerated aged seeds.Result: The deterioration was rectified to the extent possible by the technique of seed priming. SDS PAGE profiling indicated the differential expression of proteins with seed priming. Esterase and peroxidase enzyme which were completely lost as a result of ageing showed reappearance after priming. The band intensity as well as the number of proteins induced by seed priming increased over control. Priming also restored the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds. The present study concluded that priming with GA3 (100 ppm) and Ammonium Molybdate (10-3 M) for 24 hours in aged seeds of cowpea showed increase enzyme activity, restored almost entire protein profile and esterase and peroxidise isozyme profile as it allowed repair system to combat sub-cellular damage and activated synthesis of enzymes and proteins.

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