scholarly journals Using eDNA to Detect the Distribution and Density of Invasive Crayfish in the Honghe-Hani Rice Terrace World Heritage Site

2017 ◽  
Author(s):  
Wang Cai ◽  
Zhuxin Ma ◽  
Chunyan Yang ◽  
Lin Wang ◽  
Wenzhi Wang ◽  
...  

AbstractThe Honghe-Hani landscape in China is a UNESCO World Natural Heritage site due to the beauty of its thousands of rice terraces, but these structures are in danger from the invasive crayfish Procambarus clarkii. Crayfish dig nest holes, which collapse terrace walls and destroy rice production. Under the current control strategy, farmers self-report crayfish and are issued pesticide, but this strategy is not expected to eradicate the crayfish nor to prevent their spread since farmers are not able to detect small numbers of crayfish. Thus, we tested whether environmental DNA (eDNA) from paddy-water samples could provide a sensitive detection method. In an aquarium experiment, Real-time Quantitative polymerase chain reaction (qPCR) successfully detected crayfish, even at a simulated density of one crayfish per average-sized paddy (with one false negative). In a field test, we tested eDNA and bottle traps against direct counts of crayfish. eDNA successfully detected crayfish in all 25 paddies where crayfish were observed and in none of the 7 paddies where crayfish were absent. Bottle-trapping was successful in only 68% of the crayfish-present paddies. eDNA concentrations also correlated positively with crayfish counts. In sum, these results suggest that single samples of eDNA are able to detect small crayfish populations, but not perfectly. Thus, we conclude that a program of repeated eDNA sampling is now feasible and likely reliable for measuring crayfish geographic range and for detecting new invasion fronts in the Honghe Hani landscape, which would inform regional control efforts and help to prevent the further spread of this invasive crayfish.


PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0177724 ◽  
Author(s):  
Wang Cai ◽  
Zhuxin Ma ◽  
Chunyan Yang ◽  
Lin Wang ◽  
Wenzhi Wang ◽  
...  


2021 ◽  
Vol 49 (4) ◽  
pp. 1-13
Author(s):  
Feifei Yang ◽  
Anmin Huang ◽  
Jie Huang

We examined the influence of tourists' sensory experiences on their destination loyalty, and the mediating effects of tourists' emotions and memories of their experience. Data were collected using a self-report survey from 304 tourists visiting Wuyi Mountain, a natural and cultural World Heritage Site in China. We found positive impacts of sensory experiences on emotions, memories, and loyalty; of emotions on memories and loyalty; and of experience memories on loyalty. Further, sensory experiences increased tourists' loyalty by positively influencing their memories, and sensory experiences positively affected tourists' memories by arousing their emotions, thereby affecting their loyalty. Our findings provide a deeper understanding of the internal mechanism of stimulating sensory experiences for enhancing tourist loyalty. Avenues for engaging tourists should address the effect of sensory experiences on emotions and destination memories.



Diversity ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 292
Author(s):  
Martha Valdez-Moreno ◽  
Manuel Mendoza-Carranza ◽  
Eduardo Rendón-Hernández ◽  
Erika Alarcón-Chavira ◽  
Manuel Elías-Gutiérrez

This study is focused on the aquatic environments of the Sian Ka’an reserve, a World Heritage Site. We applied recently developed protocols for the rapid assessment of most animal taxa inhabiting any freshwater system using light traps and DNA barcodes, represented by the mitochondrial gene Cytochrome Oxidase I (COI). We DNA barcoded 1037 specimens comprising mites, crustaceans, insects, and fish larvae from 13 aquatic environments close or inside the reserve, with a success rate of 99.8%. In total, 167 barcode index numbers (BINs) were detected. From them, we identified 43 species. All others remain as a BIN. Besides, we applied the non-invasive method of environmental DNA (eDNA) to analyze the adult fish communities and identified the sequences obtained with the Barcode of Life Database (BOLD). All round, we found 25 fish species and other terrestrial vertebrates from this region. No alien species was found. After comparing the BINs from all systems, we found that each water body was unique with respect to the communities observed. The reference library presented here represents the first step for future programs to detect any change in these ecosystems, including invasive species, and to improve the knowledge of freshwater zooplankton, enhancing the task of compiling the species barcodes not yet stored in databases (such as BOLD or GenBank).



Author(s):  
Martha Valdez Moreno ◽  
Manuel Mendoza Carranza ◽  
Eduardo Rendón-Hernández ◽  
Erika Alarcón-Chavira ◽  
Manuel Elías-Gutiérrez

This study is focused to the aquatic environments of the Sian Ka’an reserve, a World Heritage Site. We applied protocols recently developed for the rapid assessment of most animal taxa inhabiting any freshwater system, by using light traps, and DNA barcodes, represented by the mitochondrial gene Cytochrome Oxidase I (COI). We DNA barcoded 1037 specimens of mites, crustaceans, insects, and fish larvae from 13 aquatic environments close or inside the reserve, with a success rate of 99.8%. In total, 167 Molecular Operational Taxonomic Units (MOTU’s) were detected. From them we identified 43 species. All others remain as a MOTU. For analyzing the adult fish communities, we applied the non-invasive method of environmental DNA (eDNA), and identified the sequences obtained with the Barcode of Life Database (BOLD). We found 25 fish species, and other terrestrial vertebrates from this region. No alien species was found. After a comparison of the MOTU’s from all systems, we found that each water body was unique respect the communities observed. The reference library presented here represents the first step for future programs to detect any change in these ecosystems, including invasive species, or improve knowledge of freshwater zooplankton, because most of the MOTU’s are possibly new species to science.



2010 ◽  
Vol 2 (2) ◽  
pp. 63-72
Author(s):  
Dessy Kania

Tourism is an important component of the Indonesian economy as well as a significant source of the country’s foreign exchange revenues. According to the Center of Data and Information - Ministry of Culture and Tourism, the growth of foreign visitor arrivals to Indonesia has increased rapidly by 9.61 percent since 2010 to the present. One of the most potential tourism destinations is Komodo Island located in East Nusa Tenggara. With the island’s unique qualities, which include the habitat of the Komodo dragons and beautiful and exotic marine life, it is likely to be one of the promising tourism destinations in Indonesia and in the world. In 1986, the island has been declared as a World Heritage Site by UNESCO. The Ministry of Culture and Tourism continuously promotes many of the country’s natural potential in tourism through various media: printed media, television and especially new media. However, there are challenges for the Indonesian tourism industry in facilitating entrepreneurship skills among the local people in East Nusa Tenggara. According to the Central Bureau of Statistics (2011), East Nusa Tenggara is considered as one of the poorest provinces in Indonesia where the economy is lower than the average, with a high inflation of 15%, and unemployment of 30%. This research is needed to explore further the phenomenon behind the above facts, aiming at examining the role of new media in facilitating entrepreneurship in the tourism industry in Komodo Island. The results of this study are expected to provide insights that can help local tourism in East Nusa Tenggara. Keywords: Tourism, Entrepreneurship, New Media





2018 ◽  
Vol 16 (5) ◽  
Author(s):  
Rohayah Che Amat

This research presents the value of historic urban landscape (HUL) elements in influencing the character of George Town UNESCO World Heritage Site (WHS), Penang, Malaysia. The values were perceived by the local community of different social-cultural groups that occupied the study area. The historic urban landscape elements constitute towards the protection of its townscape. The identification of the heritage elements influenced by the community interaction with their environment. This study also helps to define the character of a place, as well as reflecting its historical significance. The study adopted four techniques to gather both qualitative and quantitative data, including questionnaire survey, in-depth interview, visual survey and content analysis. In general, the local community has the capability in valuing the historic urban landscape values. The outcomes of their perceptions became the statement of the historic urban landscape values, which are expected to lead to the development of the areas. The community evaluation and perception can be expanded in implementing any development of the historic urban area by the authority.



2007 ◽  
Vol 22 (10) ◽  
pp. 2848-2852 ◽  
Author(s):  
B. Anjan Kumar Prusty ◽  
Rachna Chandra ◽  
P.A. Azeez ◽  
L.L. Sharma


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