scholarly journals The physical boundaries of public goods cooperation between surface-attached bacterial cells

2017 ◽  
Author(s):  
Michael Weigert ◽  
Rolf Kümmerli
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Soft Tissue ◽  
Social Interactions ◽  
Single Cell ◽  
Fluorescent Microscopy ◽  
Social Consequences ◽  
Social Effects ◽  
Bacterial Cells ◽  
Soft Tissue Infections ◽  
Natural Conditions

AbstractBacteria secrete a variety of compounds important for nutrient scavenging, competition mediation and infection establishment. While there is a general consensus that secreted compounds can be shared and therefore have social consequences for the bacterial collective, we know little about the physical limits of such bacterial social interactions. Here, we address this issue by studying the sharing of iron-scavenging siderophores between surface-attached microcolonies of the bacterium Pseudomonas aeruginosa. Using single-cell fluorescent microscopy, we show that siderophores, secreted by producers, quickly reach non-producers within a range of 100 μm, and significantly boost their fitness. Producers in turn respond to variation in sharing efficiency by adjusting their pyoverdine investment levels. These social effects wane with larger cell-to-cell distances and on hard surfaces. Thus, our findings reveal the boundaries of compound sharing, and show that sharing is particularly relevant between nearby yet physically separated bacteria on soft surfaces, matching realistic natural conditions such as those encountered in soft tissue infections.

scholarly journals The physical boundaries of public goods cooperation between surface-attached bacterial cells

2017 ◽  
Vol 284 (1858) ◽  
pp. 20170631 ◽  
Author(s):  
Michael Weigert ◽  
Rolf Kümmerli
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Soft Tissue ◽  
Fluorescence Microscopy ◽  
Social Interactions ◽  
Single Cell ◽  
Social Consequences ◽  
Social Effects ◽  
Bacterial Cells ◽  
Soft Tissue Infections ◽  
Natural Conditions

Bacteria secrete a variety of compounds important for nutrient scavenging, competition mediation and infection establishment. While there is a general consensus that secreted compounds can be shared and therefore have social consequences for the bacterial collective, we know little about the physical limits of such bacterial social interactions. Here, we address this issue by studying the sharing of iron-scavenging siderophores between surface-attached microcolonies of the bacterium Pseudomonas aeruginosa . Using single-cell fluorescence microscopy, we show that siderophores, secreted by producers, quickly reach non-producers within a range of 100 µm, and significantly boost their fitness. Producers in turn respond to variation in sharing efficiency by adjusting their pyoverdine investment levels. These social effects wane with larger cell-to-cell distances and on hard surfaces. Thus, our findings reveal the boundaries of compound sharing, and show that sharing is particularly relevant between nearby yet physically separated bacteria on soft surfaces, matching realistic natural conditions such as those encountered in soft tissue infections.

scholarly journals Effects of PslG on the Surface Movement of Pseudomonas aeruginosa

2018 ◽  
Vol 84 (13) ◽  
Author(s):  
Jingchao Zhang ◽  
Jing He ◽  
Chunhui Zhai ◽  
Luyan Z. Ma ◽  
Lichuan Gu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Single Cell ◽  
Human Society ◽  
Bacterial Cells ◽  
Content Type ◽  
Surface Movement ◽  
Bacterial Surface ◽  
Biofilm Control ◽  
Preferred Direction ◽  
Biofilm Development

ABSTRACT PslG attracted a lot of attention recently due to its great potential abilities in inhibiting biofilms of Pseudomonas aeruginosa . However, how PslG affects biofilm development still remains largely unexplored. Here, we focused on the surface motility of bacterial cells, which is critical for biofilm development. We studied the effects of PslG on bacterial surface movement in early biofilm development at a single-cell resolution by using a high-throughput bacterial tracking technique. The results showed that compared with no exogenous PslG addition, when PslG was added to the medium, bacterial surface movement was significantly (4 to 5 times) faster and proceeded in a more random way with no clear preferred direction. A further study revealed that the fraction of walking mode increased when PslG was added, which then resulted in an elevated average speed. The differences of motility due to PslG addition led to a clear distinction in patterns of bacterial surface movement and retarded microcolony formation greatly. Our results provide insight into developing new PslG-based biofilm control techniques. IMPORTANCE Biofilms of Pseudomonas aeruginosa are a major cause for hospital-acquired infections. They are notoriously difficult to eradicate and pose serious health hazards to human society. So, finding new ways to control biofilms is urgently needed. Recent work on PslG showed that PslG might be a good candidate for inhibiting/disassembling biofilms of Pseudomonas aeruginosa through Psl-based regulation. However, to fully explore PslG functions in biofilm control, a better understanding of PslG-Psl interactions is needed. Toward this end, we examined the effects of PslG on the surface movement of Pseudomonas aeruginosa in this work. The significance of our work is in greatly enhancing our understanding of the inhibiting mechanism of PslG on biofilms by providing a detailed picture of bacterial surface movement at a single-cell level, which will allow a full understanding of PslG abilities in biofilm control and thus present potential applications in biomedical fields.

Treatment of skin and soft tissue infections caused by Pseudomonas aeruginosa —A review of our experiences with citric acid over the past 20 years

2017 ◽  
Vol 19 ◽  
pp. 5-9 ◽  
Author(s):  
Basavraj Nagoba ◽  
Milind Davane ◽  
Rajan Gandhi ◽  
Bharat Wadher ◽  
Namdev Suryawanshi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Citric Acid ◽  
Soft Tissue ◽  
Soft Tissue Infections ◽  
The Past

scholarly journals Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin forPseudomonasandBacillusCell Imaging

2010 ◽  
Vol 2010 ◽  
pp. 1-3
Author(s):  
V. Sujatha ◽  
Bharat Sridhar ◽  
Srinath Krishnamurthy ◽  
K. S. Vinod Kumar ◽  
K. Senthil Kumar ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Fluorescent Microscopy ◽  
Water Soluble ◽  
Bacterial Cells

The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria:Pseudomonas aeruginosaandBacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness forBacillus cereus.

scholarly journals A Comparison of Pathogens in Skin and Soft Tissue Infections and Pedal Osteomyelitis in Puncture Wound Injuries Affecting the Foot

2020 ◽  
Author(s):  
David H Truong ◽  
Javier La Fontaine ◽  
Matthew Malone ◽  
Dane K Wukich ◽  
Kathryn E Davis ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Bacterial Pathogen ◽  
Soft Tissue Infections ◽  
Puncture Wound ◽  
Common Pathogen ◽  
Patients With Diabetes

ObjectiveTo compare pathogens involved in skin and soft tissue infection (SSTI) and pedal osteomyelitis (OM) in patients with and without diabetes with puncture wounds to the foot. MethodsWe evaluated 113 consecutive patients between June 2011 and March 2019 with foot infection (SSTI and OM) from a puncture injury sustained to the foot. Eighty-three patients had diabetes (DM) and 30 did not (NDM). We evaluated the bacterial pathogens in patients with skin and soft tissue infections (SSTI) and pedal osteomyelitis (OM). ResultsPolymicrobial infection were more common in patients with diabetes mellitus (83.1% vs 53.3%, p=.001). The most common pathogen for SSTI and OM in DM was s. aureus (SSTI 50.7%, OM 32.3%), whereas in NDM patients it was Pseudomonas (25%) for SSTI. Anaerobes (9.4%) and fungal (3.1%) infection were uncommon. Pseudomonas aeruginosa was only identified in 5.8% of people with diabetes. ConclusionsThe most common bacterial pathogen in both SSTIs and pedal OM was staphylococcus aureus in patients with DM. Pseudomonas spp., was the most common pathogen in people without diabetes with SSTIs.

Case Report: When all claims that it is necrotizing fasciitis but Point of care ultrasound (POCUS) proves the opposite!

POCUS Journal ◽  
2018 ◽  
Vol 3 (1) ◽  
pp. 13-14
Author(s):  
Hadiel Kaiyasah, MD, MRCS (Glasgow), ABHS-GS ◽  
Maryam Al Ali, MBBS
Keyword(s):  
Emergency Department ◽  
Critical Care ◽  
Case Report ◽  
Soft Tissue ◽  
Necrotizing Fasciitis ◽  
Soft Tissue Infection ◽  
Critical Care Unit ◽  
Point Of Care ◽  
Point Of Care Ultrasound ◽  
Soft Tissue Infections

Soft tissue ultrasound (ST-USS) has been shown to be of utmost importance in assessing patients with soft tissue infections in the emergency department or critical care unit. It aids in guiding the management of soft tissue infection based on the sonographic findings.

Sign in / Sign up

Export Citation Format

Share Document