scholarly journals VolPy: automated and scalable analysis pipelines for voltage imaging datasets

Author(s):  
Changjia Cai ◽  
Johannes Friedrich ◽  
Eftychios A Pnevmatikakis ◽  
Kaspar Podgorski ◽  
Andrea Giovannucci

AbstractVoltage imaging enables monitoring neural activity at sub-millisecond and sub-compartment scale, and therefore opens the path to studying sub-threshold activity, synchrony, and network dynamics with unprecedented spatio-temporal resolution. However, high data rates (>800MB/s) and low signal-to-noise ratios have created a severe bottleneck for analysis of such datasets. Here we present VolPy, the first turn-key, automated and scalable pipeline to pre-process voltage imaging datasets. VolPy features fast motion correction, memory mapping, segmentation, and spike inference, all built on a highly parallelized and computationally efficient framework that optimizes memory and speed. Given the lack of single cell voltage imaging ground truth examples, we introduce a corpus of 24 manually annotated datasets from different preparations and voltage indicators. We benchmark VolPy against this corpus and electrophysiology recordings, demonstrating excellent performance in neuron localization, spike extraction, and scalability.

2021 ◽  
Vol 17 (4) ◽  
pp. e1008806 ◽  
Author(s):  
Changjia Cai ◽  
Johannes Friedrich ◽  
Amrita Singh ◽  
M. Hossein Eybposh ◽  
Eftychios A. Pnevmatikakis ◽  
...  

Voltage imaging enables monitoring neural activity at sub-millisecond and sub-cellular scale, unlocking the study of subthreshold activity, synchrony, and network dynamics with unprecedented spatio-temporal resolution. However, high data rates (>800MB/s) and low signal-to-noise ratios create bottlenecks for analyzing such datasets. Here we present VolPy, an automated and scalable pipeline to pre-process voltage imaging datasets. VolPy features motion correction, memory mapping, automated segmentation, denoising and spike extraction, all built on a highly parallelizable, modular, and extensible framework optimized for memory and speed. To aid automated segmentation, we introduce a corpus of 24 manually annotated datasets from different preparations, brain areas and voltage indicators. We benchmark VolPy against ground truth segmentation, simulations and electrophysiology recordings, and we compare its performance with existing algorithms in detecting spikes. Our results indicate that VolPy’s performance in spike extraction and scalability are state-of-the-art.


2018 ◽  
Vol 7 (1.8) ◽  
pp. 245
Author(s):  
Jayakumari J ◽  
Rakhi K J

With the widespread effective usage of LEDs the visible light communication (VLC) system has brought out an increasing interest in the field of wireless communication recently. VLC is envisioned to be an appealing substitute to RF systems because of the advantages of LEDs such as high communication security, rich spectrum, etc. For achieving bearable inter symbol interference (ISI) and high data rates, OFDM can be employed in VLC. In this paper, the performance of VLC system with popular unipolar versions of OFDM viz. Flip-OFDM and ACO-OFDM is analyzed in fading channels. From the simulation results it is seen that the Flip-OFDM-VLC system outperforms the ACO-OFDM-VLC system in terms of bit error rate and is well suited for future 5G applications.


2014 ◽  
Vol 08 (02) ◽  
pp. 209-227 ◽  
Author(s):  
Håkon Kvale Stensland ◽  
Vamsidhar Reddy Gaddam ◽  
Marius Tennøe ◽  
Espen Helgedagsrud ◽  
Mikkel Næss ◽  
...  

There are many scenarios where high resolution, wide field of view video is useful. Such panorama video may be generated using camera arrays where the feeds from multiple cameras pointing at different parts of the captured area are stitched together. However, processing the different steps of a panorama video pipeline in real-time is challenging due to the high data rates and the stringent timeliness requirements. In our research, we use panorama video in a sport analysis system called Bagadus. This system is deployed at Alfheim stadium in Tromsø, and due to live usage, the video events must be generated in real-time. In this paper, we describe our real-time panorama system built using a low-cost CCD HD video camera array. We describe how we have implemented different components and evaluated alternatives. The performance results from experiments ran on commodity hardware with and without co-processors like graphics processing units (GPUs) show that the entire pipeline is able to run in real-time.


2019 ◽  
Vol 109 (11-12) ◽  
pp. 828-832
Author(s):  
M. Weigold ◽  
A. Fertig ◽  
C. Bauerdick

Durch zunehmende Vernetzung und Digitalisierung von Werkzeugmaschinen und Automatisierungskomponenten ergibt sich die Möglichkeit, Signale mit hohen Datenraten und großer Vielfalt aufzuzeichnen. Der vorliegende Beitrag beschreibt erste Untersuchungen zur Realisierbarkeit einer prozessparallelen Detektion von Bauteilfehlern auf Basis interner Werkzeugmaschinendaten. Dabei werden Potenziale und Grenzen für diesen neuartigen Ansatz zur hauptzeitparallelen Qualitätssicherung aufgezeigt.   The increasing networking and digitization of machine tools and automation components provides the opportunity to record signals with high data rates and great diversity. This paper describes first investigations on the feasibility of a process-parallel detection of component defects on the basis of internal machine tool data. Potentials and limits for this novel approach to quality assurance parallel to machining time are presented.


2019 ◽  
Vol 40 (4) ◽  
pp. 463-472
Author(s):  
Ankita Rani ◽  
Deepak Kedia

Abstract Optical code division multiple access (OCDMA) system provides highly secured transmission in addition to its capabilities to support asynchronous transmission and soft blocking. Two-dimensional OCDMA codes are chosen in this paper due to their high cardinality, good spectral efficiency and flexibility in their construction methods against direct sequence codes. This paper focuses on study of impact of multiple access interference (MAI) at high data rates for 2-D prime codesbased OCDMA system. It is shown that how the change in number of simultaneous users affects the transmission quality. Further, performance of the system is analysed in terms of bit error rate, received electrical power and eye openings. In addition, this paper also shows a comparative analysis of 2-D codes-based OCDMA system and wavelength division multiple access (WDMA) system in order to justify the improved performance of the proposed system. It is observed that the performance of 2-D OCDMA system is much better than WDMA system in terms of quality as well as security.


2007 ◽  
Author(s):  
J. Heffner ◽  
M. Mathis ◽  
B. Chandler
Keyword(s):  

2020 ◽  
Author(s):  
Wiem Abderrahim ◽  
Osama Amin ◽  
Mohamed-Slim Alouini ◽  
Basem Shihada

Next-generation communication networks are expected to integrate newly-used technologies in a smart way to ensure continuous connectivity in rural areas and to alleviate the traffic load in dense regions. The prospective access network in 6G should hinge on satellite systems to take advantage of their wide coverage and high capacity. However, adopting satellites in 6G could be hindered because of the {additional latency introduced}, which is not tolerable by all traffic types. Therefore, we propose a traffic offloading scheme that integrates both the satellite and terrestrial networks to smartly allocate the traffic between them while satisfying different traffic requirements. Specifically, the proposed scheme offloads the Ultra-Reliable Low Latency Communication (URLLC) traffic to the terrestrial backhaul to satisfy its stringent latency requirement. However, it offloads the enhanced Mobile Broadband (eMBB) traffic to the satellite since eMBB needs high data rates but is not always sensitive to delay. Our scheme is shown to reduce the transmission delay of URLLC packets, decrease the number of dropped eMBB packets, and hence improve the network's availability. Our findings highlight that the inter-working between satellite and terrestrial networks is crucial to mitigate the expected high load on the limited terrestrial capacity.<br>


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