scholarly journals Improving the quality of venous blood sampling procedure (phlebotomy): avoiding tourniquet use

Author(s):  
Francisco Freitas ◽  
Mónica Alves

AbstractBackgroundGuidelines for venous blood sampling procedure (phlebotomy) discourage tourniquet use whenever possible. Here, we aimed to assess the Biomedical Scientists capability of not using the tourniquet in phlebotomy, which we hypothesized to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site.Materials and MethodsWe selected and assigned two (BMS) with the same age (41 years) and experience (20 years) to record ten phlebotomy days, the first with prioritized and the latter with non-prioritized patients. In a simple record form, each acquired daily data for the number of attended patients, age and gender, the frequency of non-tourniquet usage and the punctured vein. To test our work hypothesis we used the two-tailed single sample t-test (p < 0.05). Differences between age-group means and non-tourniquet use means by each BMS were tested by two-tailed t-test for independent means (p < 0.05).ResultsIn 10 phlebotomy days 683 patients were attended, with males representing 43,2% of the population. We found no statistically difference between age-group means. The combined capability of non-tourniquet use was 50,5%, which did not differ from our null hypothesis, but the individual group-means were statistically different, being 33% and 66.9% in the prioritized vs non-prioritized group. The medial cubital vein was the most prone to be punctured (77,7%).ConclusionsWe have shown that performing phlebotomies without tourniquet use is possible and desirable in at least half of the attended patients, though being more limited in specific group populations. Our results provide room for quality improvement in the laboratory pre-analytical phase.Key points summaryWe assessed the capability of Biomedical Scientists not using the tourniquet in real life blood sampling procedures for diagnostic purposes.Blood was collected from at least half of the attended patients without tourniquet use.Biomedical Scientists were able to prioritize the antecubital veins without tourniquet application (medial cubital vein the most prone to be punctured - 78% of attempts).

Author(s):  
Francisco Freitas ◽  
Mónica Alves

Abstract Background Phlebotomy guidelines discourage tourniquet use whenever possible. We assessed phlebotomists' capability of not using the tourniquet in venous blood sampling, hypothesizing it to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site. Materials and Methods We assigned two phlebotomists of the same age (41 years) and experience (20 years) to record 10 phlebotomy days, the first with prioritized and the latter with nonprioritized patients. Each acquired daily data for the number of attended patients, age, gender, frequency of nontourniquet usage, and punctured vein. To test our work hypothesis we used the two-tailed single sample t-test. Differences between age-group means and nontourniquet use means by each phlebotomist were tested by two-tailed t-test for independent means. Results In 10 phlebotomy days, 683 patients were attended (males 43.2%). We found no statistically significant difference between age-group means. The combined capability of nontourniquet use was 50.5%, which did not differ from our null hypothesis, but the difference in individual group means was statistically significant, the means being 33% and 66.9% (prioritized vs. nonprioritized). The medial cubital vein was the most prone to be punctured (77.7%). Conclusion Performing phlebotomies without tourniquet was possible in at least half of the attended patients, though it was more limited in specific group populations.


2015 ◽  
Vol 2 (1) ◽  
pp. 050-054
Author(s):  
Tri Winarti

Newborns in hospitals often have a variety of invasive procedures, so that the baby is experiencingpain. Baby’s sense of discomfort and fear which will result from the pain can be observed throughthe tears. While wrapping the newborn to fit in a blanket (swaddling) can soothe a fussy baby or cry. Thepurpose of this study was to identify the effect of swaddling on pain response in neonates after venousblood sampling procedures in hospitals Ngudi Waluyo Wlingi. This study was descriptive. Samplestaken by accidental sampling technique with a sample of 35 neonates. The results showed that swaddledneonates after venous blood sampling procedure was 80% (28 respondents) pulse between 120–160times per minute, 100% (35 respondents) respiration between 30–60 times per minute and 51.4% (18respondents) SaO2 is greater than 92%. Behavioral responses such as shrill and loud cry was 8.4% (24respondents), stretching in some facial muscles and facial muscles stretching along each was 4.2% (12respondents), a strong move is 5.95% (17 respondents ), and increased muscle tone face with flexion ofthe fingers and toes was 7.7% (22 respondents).Use of swaddling post venous blood sampling proceduresin neonates can be used as a way of making neonates feel comfortable and calm.


2018 ◽  
Vol 56 (12) ◽  
pp. 2015-2038 ◽  
Author(s):  
Ana-Maria Simundic ◽  
Karin Bölenius ◽  
Janne Cadamuro ◽  
Stephen Church ◽  
Michael P. Cornes ◽  
...  

Abstract This document provides a joint recommendation for venous blood sampling of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE) and Latin American Working Group for Preanalytical Phase (WG-PRE-LATAM) of the Latin America Confederation of Clinical Biochemistry (COLABIOCLI). It offers guidance on the requirements for ensuring that blood collection is a safe and patient-centered procedure and provides practical guidance on how to successfully overcome potential barriers and obstacles to its widespread implementation. The target audience for this recommendation are healthcare staff members directly involved in blood collection. This recommendation applies to the use of a closed blood collection system and does not provide guidance for the blood collection with an open needle and syringe and catheter collections. Moreover, this document neither addresses patient consent, test ordering, sample handling and transport nor collection from children and unconscious patients. The recommended procedure is based on the best available evidence. Each step was graded using a system that scores the quality of the evidence and the strength of the recommendation. The process of grading was done at several face-to-face meetings involving the same mixture of stakeholders stated previously. The main parts of this recommendation are: 1) Pre-sampling procedures, 2) Sampling procedure, 3) Post-sampling procedures and 4) Implementation. A first draft of the recommendation was circulated to EFLM members for public consultation. WG-PRE-LATAM was also invited to comment the document. A revised version has been sent for voting on to all EFLM and COLABIOCLI members and has been officially endorsed by 33/40 EFLM and 21/21 COLABIOCLI members. We encourage professionals throughout Europe and Latin America to adopt and implement this recommendation to improve the quality of blood collection practices and increase patient and workers safety.


Data ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 105
Author(s):  
Martin Risch ◽  
Marc Kovac ◽  
Corina Risch ◽  
Dorothea Hillmann ◽  
Michael Ritzler ◽  
...  

Although SARS-CoV-2 antibody assays have been found to provide valid results in EDTA-anticoagulated whole blood, so far, they have not demonstrated that antibody levels in whole blood originating from capillary blood samples are comparable to antibody levels measured in blood from a venous origin. Here, blood is drawn simultaneously by capillary and venous blood sampling. Antibody titers are determined by an assay employing electrochemiluminescence (ECLIA) and SARS-CoV-2 total immunoglobulins are detected with specificity directed against the nucleocapsid antigen. Six individuals with confirmed COVID-19 and six individuals without COVID-19 are analyzed. Antibody titers in capillary venous whole blood did not show significant differences, and when corrected for hematocrit, they did not differ from the results obtained from serum. In conclusion, capillary sampled EDTA-anticoagulated whole blood seems to be an attractive alternative matrix for the evaluation of SARS-CoV-2 antibodies when employing ECLIA for detecting total antibodies directed against nucleocapsid antibodies.


2019 ◽  
Vol 73 ◽  
pp. 24-29 ◽  
Author(s):  
Sunita S. Jeawon ◽  
Lisa M. Katz ◽  
Noreen P. Galvin ◽  
Stephen D. Cahalan ◽  
Vivienne E. Duggan

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