scholarly journals Improving the Quality of Venous Blood Sampling Procedure (Phlebotomy): Avoiding Tourniquet Use

2021 ◽  
Author(s):  
Francisco Freitas ◽  
Mónica Alves
Keyword(s):  
Venous Blood ◽  
Sampling Procedure ◽  
T Test ◽  
Blood Sampling ◽  
Age Group ◽  
Daily Data ◽  
Significant Difference ◽  
The Difference ◽  
Without Tourniquet ◽  
Venous Blood Sampling

Abstract Background Phlebotomy guidelines discourage tourniquet use whenever possible. We assessed phlebotomists' capability of not using the tourniquet in venous blood sampling, hypothesizing it to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site. Materials and Methods We assigned two phlebotomists of the same age (41 years) and experience (20 years) to record 10 phlebotomy days, the first with prioritized and the latter with nonprioritized patients. Each acquired daily data for the number of attended patients, age, gender, frequency of nontourniquet usage, and punctured vein. To test our work hypothesis we used the two-tailed single sample t-test. Differences between age-group means and nontourniquet use means by each phlebotomist were tested by two-tailed t-test for independent means. Results In 10 phlebotomy days, 683 patients were attended (males 43.2%). We found no statistically significant difference between age-group means. The combined capability of nontourniquet use was 50.5%, which did not differ from our null hypothesis, but the difference in individual group means was statistically significant, the means being 33% and 66.9% (prioritized vs. nonprioritized). The medial cubital vein was the most prone to be punctured (77.7%). Conclusion Performing phlebotomies without tourniquet was possible in at least half of the attended patients, though it was more limited in specific group populations.

scholarly journals Improving the quality of venous blood sampling procedure (phlebotomy): avoiding tourniquet use

2020 ◽  
Author(s):  
Francisco Freitas ◽  
Mónica Alves
Keyword(s):  
Venous Blood ◽  
Real Life ◽  
Sampling Procedure ◽  
T Test ◽  
Blood Sampling ◽  
Age Group ◽  
The Individual ◽  
Sampling Procedures ◽  
Without Tourniquet ◽  
Venous Blood Sampling

AbstractBackgroundGuidelines for venous blood sampling procedure (phlebotomy) discourage tourniquet use whenever possible. Here, we aimed to assess the Biomedical Scientists capability of not using the tourniquet in phlebotomy, which we hypothesized to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site.Materials and MethodsWe selected and assigned two (BMS) with the same age (41 years) and experience (20 years) to record ten phlebotomy days, the first with prioritized and the latter with non-prioritized patients. In a simple record form, each acquired daily data for the number of attended patients, age and gender, the frequency of non-tourniquet usage and the punctured vein. To test our work hypothesis we used the two-tailed single sample t-test (p < 0.05). Differences between age-group means and non-tourniquet use means by each BMS were tested by two-tailed t-test for independent means (p < 0.05).ResultsIn 10 phlebotomy days 683 patients were attended, with males representing 43,2% of the population. We found no statistically difference between age-group means. The combined capability of non-tourniquet use was 50,5%, which did not differ from our null hypothesis, but the individual group-means were statistically different, being 33% and 66.9% in the prioritized vs non-prioritized group. The medial cubital vein was the most prone to be punctured (77,7%).ConclusionsWe have shown that performing phlebotomies without tourniquet use is possible and desirable in at least half of the attended patients, though being more limited in specific group populations. Our results provide room for quality improvement in the laboratory pre-analytical phase.Key points summaryWe assessed the capability of Biomedical Scientists not using the tourniquet in real life blood sampling procedures for diagnostic purposes.Blood was collected from at least half of the attended patients without tourniquet use.Biomedical Scientists were able to prioritize the antecubital veins without tourniquet application (medial cubital vein the most prone to be punctured - 78% of attempts).

scholarly journals RESPON NYERI PADA NEONATUS PASKA PENGAMBILAN DARAH SETELAH DIBEDONG DI RUANG NEONATUS RSUD NGUDI WALUYO WLINGI

2015 ◽  
Vol 2 (1) ◽  
pp. 050-054
Author(s):  
Tri Winarti
Keyword(s):  
Muscle Tone ◽  
Venous Blood ◽  
Behavioral Responses ◽  
Sampling Technique ◽  
Sampling Procedure ◽  
Blood Sampling ◽  
Invasive Procedures ◽  
Facial Muscles ◽  
Sampling Procedures ◽  
Venous Blood Sampling

Newborns in hospitals often have a variety of invasive procedures, so that the baby is experiencingpain. Baby’s sense of discomfort and fear which will result from the pain can be observed throughthe tears. While wrapping the newborn to fit in a blanket (swaddling) can soothe a fussy baby or cry. Thepurpose of this study was to identify the effect of swaddling on pain response in neonates after venousblood sampling procedures in hospitals Ngudi Waluyo Wlingi. This study was descriptive. Samplestaken by accidental sampling technique with a sample of 35 neonates. The results showed that swaddledneonates after venous blood sampling procedure was 80% (28 respondents) pulse between 120–160times per minute, 100% (35 respondents) respiration between 30–60 times per minute and 51.4% (18respondents) SaO2 is greater than 92%. Behavioral responses such as shrill and loud cry was 8.4% (24respondents), stretching in some facial muscles and facial muscles stretching along each was 4.2% (12respondents), a strong move is 5.95% (17 respondents ), and increased muscle tone face with flexion ofthe fingers and toes was 7.7% (22 respondents).Use of swaddling post venous blood sampling proceduresin neonates can be used as a way of making neonates feel comfortable and calm.

scholarly journals The Effect of Balloon Inflating Distraction on Pain Intensity During Venous Blood Sampling in Children: A Randomized Controlled Trial

2021 ◽  
Author(s):  
Somaeh. Salehi Sarbijan ◽  
Roghiah Mehdipoor Rabori ◽  
Esmat Nouhi
Keyword(s):  
Venous Blood ◽  
Blood Sampling ◽  
Control Group ◽  
Negative Consequences ◽  
Randomized Controlled ◽  
Pain Scores ◽  
Significant Difference ◽  
Average Pain ◽  
And Control ◽  
Venous Blood Sampling

Abstract Background: A large number of children are subject to prevention, diagnosis, and treatment procedures that mostly require venous blood sampling. Painful procedures such as blood sampling in children have annoying physical and emotional effects and can lead to wider negative consequences such as physical, behavioral, and social disorders in children. This study aimed to determine the effect of distraction by balloon inflating on the amount of pain during taking blood samples from children aged 4 to 7. Method: This study had a randomized controlled clinical trial that was conducted on 80 children admitted to the pediatric department of Imam Khomeini Hospital in Jiroft-Kerman province who met the inclusion criteria after obtaining their parent’s consent. The children were randomly grouped into the intervention (n = 40) and control (n = 40) groups. The pain level in children in the intervention group was measured during the venous blood sampling while the children were watching inflating a balloon. However, the pain in children in the control group was measured without any intervention during blood sampling. Wong–Baker Faces Pain Rating Scale questionnaire was used for pain assessment. Results: The average pain scores for the children in the two groups showed no significant difference before the intervention but the average pain scores after the intervention in the intervention and control groups were 1.38±2.56 and 4.2±1.58; showing a significant difference between the two groups after the intervention (p-value < 0.05). Conclusion: Using balloon inflation distraction during venous blood sampling reduces pain in children.

The Comparison of Hemoglobin Values Measured by Blood and Continuous Non-Invasive Monitoring (SpHb) in Newborn Infants

2020 ◽  
Author(s):  
Halil Kazanasmaz ◽  
Mahmut Demir
Keyword(s):  
Neonatal Intensive Care ◽  
Venous Blood ◽  
Newborn Infants ◽  
Blood Sampling ◽  
Neonatal Intensive Care Units ◽  
P Value ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
The Difference ◽  
Venous Blood Sampling

Abstract Objective Hemoglobin (Hb) measurement is one of the most commonly used laboratory tests in medical practice. Unnecessary blood sampling, especially in neonatal intensive care units (NICUs), contributes to iatrogenic anemia. Continuous non-invasive monitoring of total Hb (SpHb) was compared with invasive venous blood samples (tHb) in NICU patients. Methods Three hundred and ten patients were identified in NICU. Non-invasive Hb measurement was performed immediately before venous blood sampling and comparison of invasive with non-invasive values was undertaken. Results There was a strongly positive correlation between SpHb and tHb (r = 0.965, p &lt; 0.001). Bland–Altman analysis was performed in 95% limits of agreement for Hb values measured by both methods. The mean bias between tHb and SpHb measurements was 0.05 g/dl (−1.85 to 1.96). In Passing–Bablok regression analysis, the CUSUM test p value was found to be 0.98 for Hb levels measured by SpHb and tHb; and the difference between the methods was not significant. Conclusion In newborns, SpHb method offers reliable Hb values, which are comparable with the more traditional tHb method. Continuous non-invasive monitoring of total Hb may help prevent unnecessary blood sampling and iatrogenic anemia. Further clinical studies are required for the effectiveness of the method in critically ill patients with circulatory disorders.

scholarly journals The results of a close follow-up of trainees to gain a good blood collection practice

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Güzin Aykal ◽  
Hatice Esen ◽  
Cemile Öz ◽  
Ayşenur Yeğin
Keyword(s):  
Clinical Laboratory ◽  
Venous Blood ◽  
Blood Sampling ◽  
Blood Collection ◽  
Research Hospital ◽  
Preanalytical Phase ◽  
Significant Difference ◽  
Collection Practice ◽  
Venous Blood Sampling

Summary Background Phlebotomy is one of the most important steps in the preanalytical phase of a clinical laboratory process. In order to decrease phlebotomy errors, this specific procedure should be taught in detail by laboratory organizations. Our study aims to practice the training program on venous blood sampling and observe the close follow-up results. Methods In this observational study, 127 students who started their summer internship in Antalya Education and Research Hospital were given a one-day theoretical phlebotomy training in accordance with the Venous Blood Sampling Guidelines. After the theoretical training, phlebotomy applications of 10 students who were working in the field of out-patient blood sampling were observed both with and without their knowledge. A comprehensive checklist related to phlebotomy was created by the trainers in Antalya Education and Research Hospital and the observers answered each question as yes or no. For the statistical analysis, IBM SPSS Statistics 21.0 was used. Results After the theoretical education, the trainees were observed but no significant difference was found between the first and the second informed observations (p = 0.125). The students were observed three times more in the following week without their knowledge. There was a statistically significant difference between the first and the third unannounced observations (p=0.001). Conclusions In order to perform phlebotomy correctly, apart from theoretical education, a close follow-up is necessary too.

Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

2018 ◽  
Vol 1 (2) ◽  
pp. 114
Author(s):  
Wahdaniah Wahdaniah ◽  
Sri Tumpuk
Keyword(s):  
Screening Test ◽  
Venous Blood ◽  
The Body ◽  
Ethylene Diamine ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ethylene Diamine Tetra Acetate ◽  
The Difference ◽  
Index Value

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk  K2EDTA dan  K3EDTA. K3EDTA  biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

Effect of smoking on cardiovascular autonomic functions

2021 ◽  
Vol 9 (2) ◽  
pp. 3780-3784
Author(s):  
P.Vani ◽  
◽  
Sharan B Singh M ◽  
Keyword(s):  
Heart Rate ◽  
Autonomic Function ◽  
T Test ◽  
Resting Heart Rate ◽  
Autonomic Functions ◽  
Mean Values ◽  
Function Tests ◽  
Significant Difference ◽  
The Mean ◽  
The Difference

Introduction: Cigarette smoking is a prime risk factor for cardiovascular morbidity and mortality. Chronic smoking results in autonomic dysfunction leading to increased cardiovascular risk in smokers. The present study was planned to study the effect of smoking on the Cardiovascular Autonomic Functions among smokers. Materials and Methods: Fifty male subjects who were in the age group of 25 to 45 years. They were grouped into 25 smokers and 25 non-smokers. The participant subjects were selected among the staff members, residents and the patients from the routine OPD in SVIMS. Prior to study, they were informed about the procedure and the purpose of the study tests and written consents were obtained from them. The Cardiovascular Autonomic Function Tests were assessed by using a POLYGRAPH which was available in the department. Results and Conclusion: After applying the ‘t’-test for the difference between the two sample means, it was observed that there was a highly significant difference between the mean values of the BMI(i.e.p<0.01) and the para-sympathetic function tests among the smokers and the non – smokers(i.e.p<0.00). The Resting Heart Rate had significantly increased and the Deep breathing difference, the postural tachycardial index (Response to standing) and the Valsalva Ratio had significantly decreased in the smokers as compared to those in the non – smokers. After applying the ‘t’-test for the difference between the two sample means, it was observed that there was no significant difference between the mean values of the Postural hypotension test (i.e. p>0.05) and that there was a highly significant difference between the mean values of the Sustained handgrip test in the smokers and the non – smokers (i.e. p<0.00). KEY WORDS: Cardiovascular autonomic function tests, Smoking, Resting heart rate.

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