scholarly journals c-Fos-MMP-9 pathway in central amygdala mediates approach motivation but not reward consumption

2020 ◽  
Author(s):  
T. Lebitko ◽  
J. Dzik ◽  
J. Jędrzejewska-Szmek ◽  
M. Chaturvedi ◽  
T. Jaworski ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Psychiatric Disorders ◽  
Molecular Mechanism ◽  
Molecular Mechanisms ◽  
Approach Motivation ◽  
Central Amygdala ◽  
Motivation And Learning ◽  
Core Symptoms ◽  
Aversive Motivation ◽  
Metalloproteinase 9

AbstractAlthough impairments of motivational and consummatory aspects of reward behavior are core symptoms of several psychiatric disorders, the underlying neural and molecular mechanisms remain poorly understood. Here we report that blocking either c-Fos or matrix metalloproteinase 9 in the central amygdala disrupts approach motivation and appetitive discrimination learning, but not reward consumption. Further, we show that manipulation of c-Fos-MMP-9 pathway does not affect aversive motivation and learning. These findings reveal molecular mechanism of motivational anhedonia.

scholarly journals Matrix Metalloproteinase-9 and Synaptic Plasticity in the Central Amygdala in Control of Alcohol-Seeking Behavior

2017 ◽  
Vol 81 (11) ◽  
pp. 907-917 ◽  
Author(s):  
Marzena Stefaniuk ◽  
Anna Beroun ◽  
Tomasz Lebitko ◽  
Olga Markina ◽  
Szymon Leski ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Central Amygdala ◽  
Seeking Behavior ◽  
Metalloproteinase 9 ◽  
Alcohol Seeking

scholarly journals Terminalia catappaExerts Antimetastatic Effects on Hepatocellular Carcinoma through Transcriptional Inhibition of Matrix Metalloproteinase-9 by Modulating NF-κB and AP-1 Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Chao-Bin Yeh ◽  
Ming-Ju Hsieh ◽  
Yih-Shou Hsieh ◽  
Ming-Hsien Chien ◽  
Pen-Yuan Lin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Matrix Metalloproteinase ◽  
Molecular Mechanisms ◽  
Nuclear Translocation ◽  
Matrix Metalloproteinase 9 ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Mrna Levels ◽  
Inhibitory Effects ◽  
Huh7 Cells ◽  
Metalloproteinase 9

High mortality and morbidity rates for hepatocellular carcinoma (HCC) in Taiwan primarily result from uncontrolled tumor metastasis. Previous studies have identified thatTerminalia catappaleaf extracts (TCE) exert hepatoprotective, antioxidative, antiinflammatory, anticancer, and antimetastatic activities. However, the effects of TCE on HCC and the underlying molecular mechanisms of its activities have yet to be fully elucidated. The present study's findings demonstrate that TCE concentration dependently inhibits human HCC migration/invasion. Zymographic and western blot analyses revealed that TCE inhibited the activities and expression of matrix metalloproteinase-9 (MMP-9). Assessment of mRNA levels, using reverse transcriptase polymerase chain reaction (PCR) and real-time PCR, and promoter assays confirmed the inhibitory effects of TCE on MMP-9 expression in HCC cells. The inhibitory effects of TCE on MMP-9 proceeded by upregulating tissue inhibitor of metalloproteinase-1 (TIMP-1), as well as suppressing nuclear translocation and DNA binding activity of nuclear factor-kappa B (NF-κB) and activating protein-1 (AP-1) on the MMP-9 promoter in Huh7 cells. In conclusion, TCE inhibits MMP-9 expression and HCC cell metastasis and, thus, has potential use as a chemopreventive agent. Its inhibitory effects are associated with downregulation of the binding activities of the transcription factors NF-κB and AP-1.

Molecular mechanisms of nitric oxide-dependent inhibition of TPA-induced matrix metalloproteinase-9 (MMP-9) in MCF-7 cells

2009 ◽  
Vol 219 (2) ◽  
pp. 276-287 ◽  
Author(s):  
Christine Jespersen ◽  
Anke Doller ◽  
El-Sayed Akool ◽  
Malte Bachmann ◽  
Roswitha Müller ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Matrix Metalloproteinase ◽  
Molecular Mechanisms ◽  
Matrix Metalloproteinase 9 ◽  
Dependent Inhibition ◽  
Metalloproteinase 9 ◽  
Mcf 7

scholarly journals Matrix Metalloproteinase-9 (MMP-9) induced disruption of intestinal epithelial tight junction barrier is mediated by NF-κB activation

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249544
Author(s):  
Rana Al-Sadi ◽  
Jessica Engers ◽  
Mohammad Haque ◽  
Steven King ◽  
Deemah Al-Omari ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Tight Junction ◽  
Intestinal Permeability ◽  
Barrier Function ◽  
Molecular Mechanisms ◽  
Intestinal Inflammation ◽  
Nuclear Translocation ◽  
Matrix Metalloproteinase 9 ◽  
Intestinal Epithelial ◽  
Metalloproteinase 9

Background Matrix Metalloproteinase-9 (MMP-9) has been shown to play a key role in mediating inflammation and tissue damage in inflammatory bowel disease (IBD). In patients with IBD, the intestinal tight junction (TJ) barrier is compromised as characterized by an increase in intestinal permeability. MMP-9 is elevated in intestinal tissue, serum and stool of patients with IBD. Previous studies from our laboratory showed that MMP-9 causes an increase in intestinal epithelial TJ permeability and that the MMP-9 induced increase in intestinal permeability is an important pathogenic factor contributing to the development of intestinal inflammation in IBD. However, the intracellular mechanisms that mediate the MMP-9 modulation of intestinal barrier function remain unclear. Aims The main aim of this study was to further elucidate the molecular mechanisms involved in MMP-9 induced increase in intestinal epithelial TJ permeability using Caco-2 monolayers as an in-vitro model system. Results MMP-9 induced increase in Caco-2 TJ permeability was associated with activation and cytoplasmic-to-nuclear translocation of NF-κB p65. Knocking-down NF-κB p65 by siRNA transfection prevented the MMP-9 induced expression of the NF-κB target gene IL-8, myosin light chain kinase (MLCK) protein expression, and subsequently prevented the increase in Caco-2 TJ permeability. In addition, the effect of MMP-9 on Caco-2 intestinal epithelial TJ barrier function was not mediated by apoptosis or necrosis. Conclusion Our data show that the MMP-9 induced disruption of Caco-2 intestinal epithelial TJ barrier function is regulated by NF-κB pathway activation of MLCK.

scholarly journals Reward Learning Requires Activity of Matrix Metalloproteinase-9 in the Central Amygdala

2013 ◽  
Vol 33 (36) ◽  
pp. 14591-14600 ◽  
Author(s):  
E. Knapska ◽  
V. Lioudyno ◽  
A. Kiryk ◽  
M. Mikosz ◽  
T. Gorkiewicz ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Reward Learning ◽  
Central Amygdala ◽  
Metalloproteinase 9
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