Evaluation of nCoV-QS (MiCo BioMed) for RT-qPCR detection of SARS-CoV-2 from nasopharyngeal samples using CDC FDA EUA qPCR kit as a gold standard: an example of the need of validation studies

AbstractBackgroundSeveral qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation due to covid19 emergency.ObjectiveWe evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold standard.ResultsWe found limitations for nCoV-QS: 1) lower sensitivity 2) lack of RNA quality control probe 3) no capacity to quantify viral load.ConclussionsValidation studies should be implemented for any SARS-CoV-2 RT-qPCR commercial kit to prevent unreliable diagnosis.

Download Full-text

Evaluation of Viasure SARS-CoV-2 RT-qPCR kit (CerTest Biotec) using CDC FDA EUA RT-qPCR kit as a gold standard

10.1101/2020.06.29.20131367 ◽

2020 ◽

Author(s):

Byron Freire-Paspuel ◽

Patricio Vega-Mariño ◽

Alberto Velez ◽

Paulina Castillo ◽

Marilyn Cruz ◽

...

Keyword(s):

Quality Control ◽

Gold Standard ◽

Evaluation Studies ◽

Rna Extraction ◽

Rna Quality ◽

Reliable Tool ◽

Control Probe ◽

Rna Quality Control

AbstractBackgroundSeveral RT-qPCR kits are available for SARS-CoV-2 diagnosis, some of them with Emergency Use Authorization (EUA) by FDA, but most of them lacking of proper evaluation studies due to covid19 emergency.ObjectiveWe evaluated Viasure RT-qPCR kit (CerTest Biotec, Spain) for SARS-CoV-2 diagnosis using CDC FDA EUA kit as gold standard.ResultsAlthough we found the lack of RNA quality control probe as the main limitation for Viasure kit, the sensitivity was up to 97.5% and specificity was 100%.ConclusionsViasure RT-qPCR kit is a reliable tool for SARS-CoV-2 diagnosis but improvement of an alternative RT-qPCR reaction for RNA extraction quality control as RNaseP is recommended.

Download Full-text

Faculty Opinions recommendation of A new yeast poly(A) polymerase complex involved in RNA quality control.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1025403.298931 ◽

2005 ◽

Author(s):

Lionel Minvielle-Sebastia

Keyword(s):

Quality Control ◽

Polymerase Complex ◽

Rna Quality ◽

Rna Quality Control ◽

New Yeast

Download Full-text

microRNA in erythrocytes

Biochemical Society Transactions ◽

10.1042/bst0380229 ◽

2010 ◽

Vol 38 (1) ◽

pp. 229-231 ◽

Cited By ~ 22

Author(s):

Andrew J. Hamilton

Keyword(s):

Quality Control ◽

Recent Work ◽

Total Content ◽

Human Erythrocytes ◽

Rna Quality ◽

Rna Quality Control ◽

Non Coding Rnas ◽

Low Levels ◽

External Signals

Mammalian erythrocytes are generally thought to lack RNA and therefore to be unable to translate new proteins in response to internal or external signals. Support for this long-standing view has accumulated from diverse studies, most of which have focused on the total content of RNA or the overall level of translation. However, more recent work on specific types of RNA has shown the presence in human erythrocytes of both Y RNA and microRNA. The latter seem particularly incongruous given that their normal role is to attenuate the translation of mRNA. Y RNA binds the Ro autoantigen which may have a role in cellular RNA quality control. Therefore the presence of both of these non-coding RNAs indicates the possible existence of other cryptic RNAs in erythrocytes. It also suggests either the existence of low levels of translation or new uncharacterized processes involving microRNA in these cells.

Download Full-text

Integrity of SRP RNA is ensured by La and the nuclear RNA quality control machinery

Nucleic Acids Research ◽

10.1093/nar/gku761 ◽

2014 ◽

Vol 42 (16) ◽

pp. 10698-10710 ◽

Cited By ~ 7

Author(s):

Eileen Leung ◽

Claudia Schneider ◽

Fu Yan ◽

Hatem Mohi-El-Din ◽

Grzegorz Kudla ◽

...

Keyword(s):

Quality Control ◽

Rna Quality ◽

Nuclear Rna ◽

Rna Quality Control ◽

Srp Rna

Download Full-text

Ribosome states signal RNA quality control

Molecular Cell ◽

10.1016/j.molcel.2021.02.022 ◽

2021 ◽

Vol 81 (7) ◽

pp. 1372-1383 ◽

Cited By ~ 1

Author(s):

Karole N. D’Orazio ◽

Rachel Green

Keyword(s):

Quality Control ◽

Rna Quality ◽

Rna Quality Control

Download Full-text

The RNA quality control pathway nonsense-mediated mRNA decay targets cellular and viral RNAs to restrict KSHV

Nature Communications ◽

10.1038/s41467-020-17151-2 ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 4

Author(s):

Yang Zhao ◽

Xiang Ye ◽

Myriam Shehata ◽

William Dunker ◽

Zhihang Xie ◽

...

Keyword(s):

Quality Control ◽

Mrna Decay ◽

Viral Rnas ◽

Rna Quality ◽

Rna Quality Control ◽

Nonsense Mediated Mrna Decay

Download Full-text

Mitochondrial RNA quality control in trypanosomes

Wiley Interdisciplinary Reviews - RNA ◽

10.1002/wrna.1638 ◽

2020 ◽

Author(s):

Inna Aphasizheva ◽

Ruslan Aphasizhev

Keyword(s):

Quality Control ◽

Mitochondrial Rna ◽

Rna Quality ◽

Rna Quality Control

Download Full-text

NMD-Based Gene Regulation—A Strategy for Fitness Enhancement in Plants?

Plant and Cell Physiology ◽

10.1093/pcp/pcz090 ◽

2019 ◽

Vol 60 (9) ◽

pp. 1953-1960 ◽

Cited By ~ 9

Author(s):

Misato Ohtani ◽

Andreas Wachter

Keyword(s):

Quality Control ◽

Gene Regulation ◽

Current Knowledge ◽

Qualitative And Quantitative ◽

Rna Quality ◽

Quantitative Level ◽

Splicing Variants ◽

Rna Quality Control ◽

Nonsense Mediated Mrna Decay ◽

Environmental Responses

Abstract Post-transcriptional RNA quality control is a vital issue for all eukaryotes to secure accurate gene expression, both on a qualitative and quantitative level. Among the different mechanisms, nonsense-mediated mRNA decay (NMD) is an essential surveillance system that triggers degradation of both aberrant and physiological transcripts. By targeting a substantial fraction of all transcripts for degradation, including many alternative splicing variants, NMD has a major impact on shaping transcriptomes. Recent progress on the transcriptome-wide profiling and physiological analyses of NMD-deficient plant mutants revealed crucial roles for NMD in gene regulation and environmental responses. In this review, we will briefly summarize our current knowledge of the recognition and degradation of NMD targets, followed by an account of NMD’s regulation and physiological functions. We will specifically discuss plant-specific aspects of RNA quality control and its functional contribution to the fitness and environmental responses of plants.

Download Full-text