scholarly journals Evaluation of nCoV-QS (MiCo BioMed) for RT-qPCR detection of SARS-CoV-2 from nasopharyngeal samples using CDC FDA EUA qPCR kit as a gold standard: an example of the need of validation studies

Author(s):  
Byron Freire-Paspuel ◽  
Patricio Vega-Mariño ◽  
Alberto Velez ◽  
Paulina Castillo ◽  
Marilyn Cruz ◽  
...  

AbstractBackgroundSeveral qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation due to covid19 emergency.ObjectiveWe evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold standard.ResultsWe found limitations for nCoV-QS: 1) lower sensitivity 2) lack of RNA quality control probe 3) no capacity to quantify viral load.ConclussionsValidation studies should be implemented for any SARS-CoV-2 RT-qPCR commercial kit to prevent unreliable diagnosis.

2020 ◽  
Author(s):  
Byron Freire-Paspuel ◽  
Patricio Vega-Mariño ◽  
Alberto Velez ◽  
Paulina Castillo ◽  
Marilyn Cruz ◽  
...  

AbstractBackgroundSeveral RT-qPCR kits are available for SARS-CoV-2 diagnosis, some of them with Emergency Use Authorization (EUA) by FDA, but most of them lacking of proper evaluation studies due to covid19 emergency.ObjectiveWe evaluated Viasure RT-qPCR kit (CerTest Biotec, Spain) for SARS-CoV-2 diagnosis using CDC FDA EUA kit as gold standard.ResultsAlthough we found the lack of RNA quality control probe as the main limitation for Viasure kit, the sensitivity was up to 97.5% and specificity was 100%.ConclusionsViasure RT-qPCR kit is a reliable tool for SARS-CoV-2 diagnosis but improvement of an alternative RT-qPCR reaction for RNA extraction quality control as RNaseP is recommended.


2010 ◽  
Vol 38 (1) ◽  
pp. 229-231 ◽  
Author(s):  
Andrew J. Hamilton

Mammalian erythrocytes are generally thought to lack RNA and therefore to be unable to translate new proteins in response to internal or external signals. Support for this long-standing view has accumulated from diverse studies, most of which have focused on the total content of RNA or the overall level of translation. However, more recent work on specific types of RNA has shown the presence in human erythrocytes of both Y RNA and microRNA. The latter seem particularly incongruous given that their normal role is to attenuate the translation of mRNA. Y RNA binds the Ro autoantigen which may have a role in cellular RNA quality control. Therefore the presence of both of these non-coding RNAs indicates the possible existence of other cryptic RNAs in erythrocytes. It also suggests either the existence of low levels of translation or new uncharacterized processes involving microRNA in these cells.


2014 ◽  
Vol 42 (16) ◽  
pp. 10698-10710 ◽  
Author(s):  
Eileen Leung ◽  
Claudia Schneider ◽  
Fu Yan ◽  
Hatem Mohi-El-Din ◽  
Grzegorz Kudla ◽  
...  

2021 ◽  
Vol 81 (7) ◽  
pp. 1372-1383 ◽  
Author(s):  
Karole N. D’Orazio ◽  
Rachel Green

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yang Zhao ◽  
Xiang Ye ◽  
Myriam Shehata ◽  
William Dunker ◽  
Zhihang Xie ◽  
...  

2019 ◽  
Vol 60 (9) ◽  
pp. 1953-1960 ◽  
Author(s):  
Misato Ohtani ◽  
Andreas Wachter

Abstract Post-transcriptional RNA quality control is a vital issue for all eukaryotes to secure accurate gene expression, both on a qualitative and quantitative level. Among the different mechanisms, nonsense-mediated mRNA decay (NMD) is an essential surveillance system that triggers degradation of both aberrant and physiological transcripts. By targeting a substantial fraction of all transcripts for degradation, including many alternative splicing variants, NMD has a major impact on shaping transcriptomes. Recent progress on the transcriptome-wide profiling and physiological analyses of NMD-deficient plant mutants revealed crucial roles for NMD in gene regulation and environmental responses. In this review, we will briefly summarize our current knowledge of the recognition and degradation of NMD targets, followed by an account of NMD’s regulation and physiological functions. We will specifically discuss plant-specific aspects of RNA quality control and its functional contribution to the fitness and environmental responses of plants.


2016 ◽  
Vol 28 (2) ◽  
pp. 426-438 ◽  
Author(s):  
Emilie Elvira-Matelot ◽  
Florian Bardou ◽  
Federico Ariel ◽  
Vincent Jauvion ◽  
Nathalie Bouteiller ◽  
...  

2012 ◽  
Author(s):  
Lauren M. Stanoszek ◽  
Erin L. Crawford ◽  
Thomas Blomquist ◽  
Paige Willey ◽  
James C. Willey

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