Mass Spectrometry for O-GlcNAcylation

O-linked β-N-acetylglucosamine modification (O-GlcNAcylation) at proteins with low-abundance expression level and species diversity, shows important roles in plenty of biological processes. O-GlcNAcylations with abnormal expression levels are associated with many diseases. Systematically profiling of O-GlcNAcylation at qualitative or quantitative level is vital for their function understanding. Recently, the combination of affinity enrichment, metabolic labeling or chemical tagging with mass spectrometry (MS) have made significant contributions to structure-function mechanism elucidating of O-GlcNAcylations in organisms. Herein, this review provides a comprehensive update of MS-based methodologies for quali-quantitative characterization of O-GlcNAcylation.

The Folklore of the Calendar Customs (Spring, Summer, Autumn) Recorded in The Digitized Collections of Ethnographic and Folkloric Materials

The article informs and provides an analysis on the qualitative and quantitative level of the data regarding the folklore of the calendar customs (spring, summer, autumn), which were digitized within the project Saving the Folkloric Archival Material Preserved in Chisinau, Republic of Moldova (EAP1045). Transgressed from the classic archiving format to the digitized one, the manuscripts and cassette tapes offer us valuable information gathered between 1956-1992, with reference to the folklore of the feasts celebrated in the warm season of the year, as well as of the customs, social, ceremonial and ritual practices, beliefs and mythical representations associated with them. Evaluating the consistency of documenting these folkloric and ethnographic expressions in the archival materials, we noticed inequalities and prioritizations in their recording and researching, the attention of the folclorists being visibly diffused towards other genres of folklore.

Editorial Identities, Business Models, and Social Strategies: Spanish Women Editors in the Nineteenth and Early Twentieth Centuries

This article examines women’s periodical editorship in nineteenth- and early-twentieth-century Spain. Drawing on library collections and digital periodical databases, it revisits the pioneering research on a small number of major figures undertaken since the 1990s and tests it on a much larger scale. Was female editorship a negligible phenomenon in the history of the Spanish press, or are we only beginning to discover its scope? And if more women editors are identified, to what extent can we extrapolate insights into the profiles, networks, and strategies of a few grandes dames to larger numbers? Our approach not only enables us to answer these questions on a quantitative level, it also opens up a large corpus of periodicals for more in-depth qualitative research. Specifically, after presenting some quantitative findings and general observations, we examine three factors that played a role in the success and failure of Spanish women’s periodical editorship: editorial identities, business models, and social strategies.

Do students with different cognitive styles have similar levels of statistical thinking?

[English]: This study aims to examine and compare students’ level of statistical thinking with different cognitive styles (Field-dependent, FD and Field-independent, FI) in solving mathematical problems. It is descriptive qualitative research involving 31 ninth-graders given the Group Embedded Figure Test (GEFT) to determine their cognitive styles. From the results of GEFT, four students with two cognitive styles and high mathematics ability were selected as participants. A test and interviews were administered for data collection. The test was analyzed based on the level of statistical thinking indicators, and the interview results were used to confirm and explore the students' statistical thinking. The results of data analysis revealed that in representing data, both FI and FD students are at transitional level. In other stages of statistical thinking: describing data display, organizing and reducing data, analyzing and interpreting data, FD students reach a quantitative level meanwhile FI students are at an analytical level. Indeed, students with FI cognitive style have a higher level of statistical thinking than FD students. This finding shows that characteristics of students with FI, for example, being more analytic, support the achievement of better levels of statistical thinking. [Bahasa]: Penelitian ini bertujuan untuk mengidentifikasi dan membandingkan level berpikir statistik siswa dengan gaya kognitif berbeda (Field-dependent, FD dan Field-independent, FI) dalam memecahkan masalah matematika. Penelitian ini merupakan penelitian kualitatif deskriptif yang melibatkan 31 siswa kelas 9. Siswa tersebut diberikan Group Embedded Figure Test (GEFT) untuk menentukan gaya kognitif. Selanjutnya, dipilih 4 siswa pada kedua gaya kognitif dengan kemampuan matematika tinggi. Data dikumpulkan melalui tes dan wawancara. Hasil tes dianalisis berdasarkan indikator level berpikir statistik dan hasil wawancara dianalisis untuk mengonfirmasi dan menggali lebih dalam berpikir statistik siswa. Hasil analisis data menunjukkan bahwa dalam menyajikan data, siswa FD dan FI berada pada level transisional. Pada tiga tahap berpikir statistik lain; menjelaskan sajian data, mengatur dan mengurangi data, menganalisis dan menerjemahkan data, siswa FD berada pada level kuantitatif sedangkan siswa FI berada pada level analitik. Dalam hal ini, siswa dengan gaya kognitif FI memiliki level berpikir statistik yang lebih tinggi dibandingkan dengan siswa FD. Hasil penelitian ini menunjukkan bahwa karakteristik siswa dengan gaya kognitif FI, misalnya cenderung lebih analitis dibandingkan FD, mendukung capaian level berpikir statistik yang lebih baik.

SARS-Cov2 Infection Detection by Polymerase Chain Reaction and Serologic Testing in Clinical Practice

Patients with SARS-Cov2 can be diagnosed by polymerase chain reaction (PCR) during the time of acute infection or later in their clinical course by detection of virus specific antibodies. While in theory complementary, both PCR and serologic tests have practical shortcomings. A retrospective study was performed in order to further define these limitations in a clinical context and to determine how to best utilize these tests in a coherent fashion. A total of 3,075 patients underwent both PCR and serology tests at UCLA in the study period. Among these, 2731 (89%) had no positive tests at all, 73 (2%) had a positive PCR test and only negative serology tests, 144 (5%) had a positive serology test and only negative PCR tests, and 127 (4%) had positive PCR and serology tests. Approximately half of the patients with discordant results (i.e. PCR positive, serology negative or vice versa) had mistimed tests in reference to the course of their disease. PCR positive patients who were asymptomatic or pregnant were less likely to generate a detectable humoral immune response to SARS-Cov2. On a quantitative level, the log number of days between symptom onset and PCR cycle threshold (Ct) values were positively correlated. However, there was no apparent relationship between PCR Ct and serologic AU/ml results.

Knowledge Management Innovation For SustainableDevelopment in the Context of the Economic Crisis

The trade (qualitative and quantitative level of trade) can promote the concept of sustainable development. The concept of Sustainable Development involves the implementation of theoretical and practical components for making decisions in any situation in which features a man-type medium, be it the environment, economic or social. The goals of sustainable development include the harmonization of the economic, social and environmental targets. This paper presents the main types of the correlations: Trade – Sustainable Development – Economic Crisis. The Sustainable Development (SD) concept is also analyzed in direct correlation with the Corporate Social Responsibility (CSR) concept. The SD concept involves the implementation of theoretical and practical components for making decisions in any situation in which a man-type medium, be it the environment, economic or social features. The Corporations (qualitative and quantitative level of trade) can promote the concept of sustainable development. The goals of sustainable development include the harmonization of the economic, social and environmental targets. This paper presents the main research on the main types of the correlations: Corporate Social Responsibility (including trade) – Sustainable Development – Economic Crisis.

СOMMONALITY OF PLAUTUS 'COMEDIES AND V. KATAEV'S COMEDY «QUADRATURE OF THE CIRCLE»

Статья посвящена исследованию литературных источников драматургической техники комедии В. Катаева «Квадратура круга». Исследование формальных приемов в структуре произведения строится на текстуальном анализе трех комедий Плавта и комедии «Квадратура круга». Анализ сходства драматургической техники производится путем сопоставления отдельных сцен в комедиях Плавта «Клад», «Близнецы», «Пленники» и комедии В. Катаева «Квадратура круга». Автором статьи используется терминология, существующая в научном обороте, но не имеющая нормативного употребления. Слова «схема», «фигура», «элемент» являются синонимами и обозначают отдельный прием драматургической техники. Процесс текстуального сличения отдельных сцен комедий Плавта и Катаева демонстрирует очевидную общность драматургической техники. Сходство приемов драматургической техники, их количественный уровень указывает на факт заимствования Катаевым отдельных элементов из комедий римского автора. Драматургические фигуры, составляющие общность приемов в комедиях Плавта и Катаева, различны на идейно-тематическом и структурно-композиционном уровне. Глубина профессионального освоения творчества Плавта вкупе с дарованием и талантом самого Валентина Петровича способствовали феерическому успеху «Квадратуры круга» на российской и мировой сценах. Использование литературно-исторических традиций античной драматургии Катаевым является бесценным опытом и примером создания произведения высокого художественного уровня. The article is devoted to the study of literary sources of the dramatic technique of V. Kataev's Comedy «the Quadrature of the circle». The study of formal techniques in the structure of the work is based on the textual analysis of three comedies by Plautus and V. Kataev's Comedy «the Quadrature of the circle». The similarity of dramatic technique is analyzed by comparing individual scenes in Plautus 'comedies «the Treasure», «the Twins», «the Prisoners» and V. Kataev's Comedy «the Quadrature of the circle». The author of the article uses terminology that exists in scientific circulation but does not have normative use. Words: the schematic of figure element are synonymous and refer to the individual reception of dramatic technique. The process of textual comparison of individual scenes of Plautus and Kataev's comedies demonstrates an obvious similarity of dramatic technique. The similarity of the techniques of dramaturgical technique ,their quantitative level, indicates the fact that V. Kataev adopted certain elements from the comedies of the Roman author. The dramaturgical figures that make up the commonality of techniques in the comedies of Plautus and V. Kataev are different on the ideological-thematic and structural-compositional level. The depth of Kataev's professional development of Plautus ' work, combined with the talent and talent of Valentin Petrovich, contributed to the enchanting success of the circle Quadrature on the Russian and world stages. The use of literary and historical traditions of ancient drama By V. Kataev is an invaluable experience and example of creating a work of high artistic level.

Concentration and distribution of oil in the reservoir mineral Dolomite studied by neutron tomography

Neutron Tomography – NT is a non-destructive technique, ideal for imaging hydrogen-rich substances even when wrapped up by thick rock layers. In the present study, the NT was employed for investigating the oil concentration and distribution in the mineral Dolomite, a natural oil reservoir. Two types of Dolomite samples, one of which dry, and the second one in oil-saturated were prepared. The applied oil was EMCAplus® 070 usually employed in permeability experiments to reproduce conditions of natural oil reservoirs. Three small fragments of the dry sample and three of the oil sample were inspected by NT and the comparison between the obtained results enabled, to distinguish several regions in which the oil is stored, to indicate that the oil is dispersed throughout the volume of the samples, and to quantify the storage concentration. The obtained data for oil concentration, for the three fragments, were compared to each other and to the one obtained by the conventional gravimetric technique, and they agreed within their uncertainties, demonstrating the viability of the NT technique to inspect, either in a qualitative as in a quantitative level, this oil reservoir mineral.

Diversidad anatómica de cinco biotipos de la especie Theobroma cacao(cacao) en la región sur de Ecuador

Agrobiodiversity includes variability at the specific and genetic level, its dynamics and relationships between cultivated plants and their wild relatives. The Theo b ro m a c a c aoL. species has a high cultural and economic value inEcuador because it helps around 100,000 rural families. However, the structure of its wood and the variability of microscopic characteristics between its biotypes is unknown. For this reason, this study seeks to fill th is gap with an anatomical analysis of the wood in its three planes (transverse, tangentia l, a n d radial) according to IAWA regulations in five biotypes of T. cocoa (aromático, criollo, forastero, trinitario y CCN51) from the Piuntza and Guayzimi sectors of the province of Zamora Chinchipe. Itwas found that all biotypes presented simple perforation plates, alternating polygonal pits, diffuse axial parenchyma, multiseriate and uniseriate ra y s , ra y s with procumbent cells of 2-4 rows of vertical and / or marginal square cells,h ow e ve r, th e prismatic crystals, drusen, envelope cells, and perforated radial cells were variable. At a quantitative level, the highest radii were found mainly in the creole and fore ign b io ty pe s , while the CCN51 and trinitariobiotypes obtained higher values in vessels per square millimeter. With this, it was shown that all the individuals analyzed, despite being of the same species, had anatomical differences, which shows that the microscopic study of woo d is a very useful tool for the identification of biotypes within a species.Keywords:Wood anatomy, cacao, biotype, IAWA, agrobiodiversity

A SHORELINE EVOLUTION MODEL BASED ON EQUILIBRIUM FORMULATIONS

Traditionally, the shoreline hindcast under the influence of changing marine conditions has been considered by means of existing robust shoreline evolution models, such as one-line, multi-line, combined or 3D models. All of them require long data series, many calibration parameters and are computationally intensive. This study presents a new shoreline evolution model considering the integration of cross-shore, planform and rotation equilibrium-based models, applicable over time-scales spanning days, months or several years. The new model successfully reaches the general erosion-accretion trend at a qualitative and quantitative level. As the main conclusion, this is a simple equilibrium-based shoreline evolution model that requires few calibration parameters and is computationally efficient and versatile.Recorded Presentation from the vICCE (YouTube Link): https://youtu.be/zkQ7AoAWmEE