scholarly journals The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

2020 ◽  
Author(s):  
Farhad Shafiei ◽  
Mathew P. Watts ◽  
Lukas Pajank ◽  
John W. Moreau
Keyword(s):  
Heavy Metals ◽  
Mine Tailings ◽  
Energy Demand ◽  
High Efficiency ◽  
Microbial Consortium ◽  
Bacterial Consortium ◽  
Process Efficiency ◽  
Microbial Consortia ◽  
List Type ◽  
Gold Mine Tailings

AbstractBioremediation systems represent an environmentally sustainable approach to degrading industrially-generated thiocyanate (SCN-), with low energy demand and operational costs, and high efficiency and substrate specificity. However, heavy metals present in mine tailings effluent may hamper process efficiency by poisoning thiocyanate-degrading microbial consortia. Here we experimentally tested the tolerance of an autotrophic SCN--degrading bacterial consortium enriched from gold mine tailings for Zn, Cu, Ni, Cr, and As. All of the selected metals inhibited SCN- biodegradation to different extents, depending on concentration. At pH of 7.8 and 30°C, complete inhibition of SCN- biodegradation by Zn, Cu, Ni, and Cr occurred at 20, 5, 10, and 6 mg L-1, respectively. Lower concentrations of these metals decreased the rate of SCN- biodegradation, with relatively long lag times. Interestingly, the microbial consortium tolerated As even at 500 mg L-1, although both the rate and extent of SCN- biodegradation were affected. This study highlights the importance of considering metal co-contamination in bioreactor design and operation for SCN- bioremediation at mine sites.Key pointsBoth the efficiency and rate of SCN- biodegradation were inhibited by heavy metals, to different degrees depending on type and concentration of metalThe autotrophic microbial consortium was capable of tolerating high levels of As

scholarly journals The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

2020 ◽  
Vol 105 (1) ◽  
pp. 417-427
Author(s):  
Farhad Shafiei ◽  
Mathew P. Watts ◽  
Lukas Pajank ◽  
John W. Moreau
Keyword(s):  
Heavy Metals ◽  
Mine Tailings ◽  
Energy Demand ◽  
High Efficiency ◽  
Microbial Consortium ◽  
Bacterial Consortium ◽  
Process Efficiency ◽  
Microbial Consortia ◽  
Gold Mine Tailings ◽  
High Concentrations

Abstract Bioremediation systems represent an environmentally sustainable approach to degrading industrially generated thiocyanate (SCN−), with low energy demand and operational costs and high efficiency and substrate specificity. However, heavy metals present in mine tailings effluent may hamper process efficiency by poisoning thiocyanate-degrading microbial consortia. Here, we experimentally tested the tolerance of an autotrophic SCN−-degrading bacterial consortium enriched from gold mine tailings for Zn, Cu, Ni, Cr, and As. All of the selected metals inhibited SCN− biodegradation to different extents, depending on concentration. At pH of 7.8 and 30 °C, complete inhibition of SCN− biodegradation by Zn, Cu, Ni, and Cr occurred at 20, 5, 10, and 6 mg L−1, respectively. Lower concentrations of these metals decreased the rate of SCN− biodegradation, with relatively long lag times. Interestingly, the microbial consortium tolerated As even at 500 mg L−1, although both the rate and extent of SCN− biodegradation were affected. Potentially, the observed As tolerance could be explained by the origin of our microbial consortium in tailings derived from As-enriched gold ore (arsenopyrite). This study highlights the importance of considering metal co-contamination in bioreactor design and operation for SCN− bioremediation at mine sites. Key points • Both the efficiency and rate of SCN−biodegradation were inhibited by heavy metals, to different degrees depending on type and concentration of metal. • The autotrophic microbial consortium was capable of tolerating high concentrations of As, potential having adapted to higher As levels derived from the tailings source.

Column Bioleaching of Arsenic and Heavy Metals from Gold Mine Tailings by Aspergillus fumigatus

2012 ◽  
Vol 40 (6) ◽  
pp. 607-614 ◽  
Author(s):  
Bahi Jalili Seh-Bardan ◽  
Radziah Othman ◽  
Samsuri Ab Wahid ◽  
Aminudin Husin ◽  
Fardin Sadegh-Zadeh
Keyword(s):  
Heavy Metals ◽  
Aspergillus Fumigatus ◽  
Mine Tailings ◽  
Gold Mine ◽  
Gold Mine Tailings

A Comparative Study on the Pollution and Speciation Characteristics of Heavy Metals between the Gold and Iron Mine Tailings of the Upstream Area of Miyun Reservoir, Beijing

2012 ◽  
Vol 518-523 ◽  
pp. 1412-1416 ◽  
Author(s):  
Xing Xing Huang ◽  
Hong Bing Ji ◽  
Cai Li ◽  
Fei Qin ◽  
Qian Li ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Sequential Extraction ◽  
Mine Tailings ◽  
Mine Tailing ◽  
Gold Mine ◽  
Miyun Reservoir ◽  
Total Metal ◽  
Iron Mine ◽  
Gold Mine Tailings

In order to have a comparative investigation of the pollution and speciation characteristics of heavy metals between the gold and iron mine tailings of the upstream areas of Miyun Reservoir, the tailing samples from the typical gold and iron mines were analyzed. The total metal concentration is determined by acid digestion and the chemical fractionation of metal by the Tessier sequential extraction method. It is found that compared to background values of Beijing, the heavy metal in both gold and iron mine tailings is higher, while the heavy metal in gold mine tailings is higher than that of iron mine tailings, with Mn as an exception. Mercury is the most serious pollution element in gold mine tailings. Results of the sequential extraction suggest that the exchangeable of Cd exhibits the highest percentage of total metal in gold and iron mine tailings（15% and 6.5% of the total metal, respectively) among Cd, Mn, Zn, Cu, Pb, Cr. In the majority of iron mine tailing samples collected, large amounts of Cu were mainly associated with the organic form. The percentage of the bioavailable metals in both gold and iron mine tailing samples follows the order: Cd>Mn>Zn>Cu>Pb>Cr.

Physicochemical properties, heavy metals, and metal-tolerant bacteria profiles of abandoned gold mine tailings in Krugersdorp, South Africa

2020 ◽  
Vol 100 (3) ◽  
pp. 217-233
Author(s):  
Muibat Omotola Fashola ◽  
Veronica Mpode Ngole-Jeme ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Physicochemical Properties ◽  
Mine Tailings ◽  
Terrestrial Ecosystems ◽  
Gold Mine ◽  
Biochemical Tests ◽  
Mining Sites ◽  
Gold Mine Tailings ◽  
Metal Tolerant Bacteria

Mine tailings are a potential source of heavy metals (HM) that can be toxic to microbes, plants, and animals in aquatic and terrestrial ecosystems. Bacteria have evolved several mechanisms to tolerate the uptake of HM ions. This study aimed to assess the physicochemical properties, concentrations of selected HM and metalloids [arsenic (As), nickel (Ni), lead (Pb), zinc (Zn), cadmium (Cd), and cobalt (Co)], and isolate potential metal-tolerant bacteria present at three abandoned gold mining sites with a view of understanding how tailings characteristics vary and the implications on microbial activities in tailings dumps. Heavy-metal-tolerant bacteria were isolated from the samples using minimum inhibitory and maximum tolerable concentrations of the Ni, Pb, Zn, Cd, and Co. The substrates of the studied sites were acidic and deficient in nutrients. High metals and metalloid concentrations in the order Zn > Ni > Co > As > Pb > Cd were recorded in some of the studied sites and its adjacent soil which exceeded South African recommended values for soil and sediments. Heavy-metal-tolerant bacteria that showed multiple tolerances to Ni, Pb, and Zn were isolated and putatively identified using biochemical tests as belonging to the phyla Proteobacteria, Actinobacteria, and Firmicutes. Gold mine tailings enriched the soil with HM and also affect soil physicochemical properties. Proper management of mine wastes must be ensured to prevent their adverse effects on the diversity, composition, and activity of soil microorganisms that help in maintenance of the ecosystem.

scholarly journals Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

2020 ◽  
Vol 141 ◽  
pp. 03005
Author(s):  
Raviporn Runajak ◽  
Santi Chuetor ◽  
Wawat Rodiahwati ◽  
Malinee Sriariyanun ◽  
Prapakorn Tantayotai ◽  
...  
Keyword(s):  
High Efficiency ◽  
Microbial Consortium ◽  
Biogas Production ◽  
Molecular Size ◽  
Microbial Consortia ◽  
Biomass Degradation ◽  
Cellulase Enzyme ◽  
Cassava Pulp ◽  
Processing Plants ◽  
Set Up

Biogas production is one of the means to manage the cassava pulp waste obtained from the cassava processing plants. The success of the process is determined by the hydrolysis in an anaerobic digester. When the digester failure is found, the new microbial consortium inoculum is introduced to the system with the long period of set up time. This research aimed to construct the endemic microbial consortium by re-cultivating the cellulolytic microbial consortia obtained from cassava pulp and digester wastewater with the expected shorter set up time. Modifications of enrichment and re-cultivation methods by varying the nutrients, pH and temperature improved the enzymatic hydrolysis yields, as reducing sugars, of CMC, rice straw and cassava pulp substrates approximately 9, 3, and 13 times, respectively. To analyze the enzymatic activities of the selected microbial consortia, the cellulase enzyme was extracted, partially purified and analyzed on CMC-zymogram. The ~130 kDa-sized cellulase enzyme was identified with endocellulase activity, and it was considered as a relatively large molecular size molecule compared to most bacterial endocellulases. The selected microbial consortia were tested for their biomass degradation capacities, and the optimal operational condition was obtained at pH 7.0 and 30 °C. This optimal condition showed the proof of the concept that this re-cultivated consortium could be applied in on-site digester with high efficiency.

Effects of humic acid amendment on the mobility of heavy metals (Co, Cu, Cr, Mn, Ni, Pb, and Zn) in gold mine tailings in Thailand

2015 ◽  
Vol 8 (9) ◽  
pp. 7589-7600 ◽  
Author(s):  
Srilert Chotpantarat ◽  
Lalita Chunhacherdchai ◽  
Rakkreat Wikiniyadhanee ◽  
Chantra Tongcumpou
Keyword(s):  
Heavy Metals ◽  
Humic Acid ◽  
Mine Tailings ◽  
Gold Mine ◽  
Gold Mine Tailings

scholarly journals Immobilization of sulfates and heavy metals in gold mine tailings by sodium silicate and hydrated lime

2018 ◽  
Vol 6 (5) ◽  
pp. 6530-6536 ◽  
Author(s):  
Jenni Kiventerä ◽  
Harisankar Sreenivasan ◽  
Christopher Cheeseman ◽  
Paivo Kinnunen ◽  
Mirja Illikainen
Keyword(s):  
Heavy Metals ◽  
Sodium Silicate ◽  
Mine Tailings ◽  
Hydrated Lime ◽  
Gold Mine ◽  
Gold Mine Tailings

scholarly journals Significant Improvement of Straw  Lignocellulose Degradation Rate by Subordination Consortium Composed of Cellulomonas ZJW-6 and Acinetobacter DA-25

2021 ◽  
Author(s):  
Yunpeng Guan ◽  
Hongyu Zhu ◽  
Yuan Zhu ◽  
Longhua Shu ◽  
Hemei Zhao ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Agricultural Development ◽  
Degradation Rate ◽  
High Efficiency ◽  
Microbial Consortium ◽  
Temperature Tolerance ◽  
Degradation Efficiency ◽  
Microbial Consortia ◽  
Lignocellulose Degradation ◽  
Glucosidase Activity

Abstract BackgroundThe effective utilization of lignocellulose is essential for achieving circular economy and sustainable agricultural development. The screening of lignocellulose-degrading strains from the environment and artificial establishment of microbial consortia can achieve better degradation effects. In this paper, microbial consortium 625 was established and screened based on high lignocellulose degradation ability but low β-glucosidase activity of Cellulomonas ZJW-6 to complement enzyme activity and enhance lignocellulose degradation by exerting the subordination effect. This research provides an effective strategy for artificially establishing microbial consortia, improving lignocellulose degradation efficiency, and enhancing bioenergy production.ResultsIn order to establish high-efficiency microbial consortia, Cellulomonas ZJW-6 with low β-glucosidase activity was combined in pairs with 14 strains with high β-glucosidase activity according to the enzyme activity complementation principle, from which microbial consortium 625 composed of strains ZJW-6 and DA-25 was screened, which had significantly enhanced lignocellulose degradation rate compared with purely cultured ZJW-6 strain and other 13 combinations. The single factor test showed that consortium 625 had better pH and temperature tolerance. The lignocellulose degradation rate of this consortium was 57.62% after verification test under optimized conditions using response surface method. The induction test showed that the growth of strain DA-25 was inhibited by strain ZJW-6 and its filtrate, while strain DA-25 and its filtrate promoted the growth of strain ZJW-6 and significantly enhanced the activities of key enzymes in the consortium 625 lignocellulose degrading system, among which the β-Glucosidase activity was significantly higher than that in purely cultured ZJW-6 strain. ConclusionMicrobial consortium 625, composed of Cellulomonas ZJW-6 (dominant strain) and Acinetobacter DA-25, with high lignocellulose degradation efficiency, was screened according to the enzyme activity complementation principle. Interestingly, microbial consortium 625 exhibited complementation in enzyme activities of its two strains, improvement in its tolerance to temperature and pH, and the same effect of DA-25 filtrate as strain DA-25. Based on the previous studies on Acinetobacter, we speculate that a certain compound adverse to the growth of strain DA-25 may be produced during lignocellulose degradation by strain ZJW-6, while secretions of strain DA-25 inhibit this compound leading to an increase in the growth and enzyme activity of strain ZJW-6.

Biosorption of Heavy Metals in Leachate Derived from Gold Mine Tailings UsingAspergillus fumigatus

2012 ◽  
Vol 41 (4) ◽  
pp. 356-364 ◽  
Author(s):  
Bahi Jalili Seh-Bardan ◽  
Radziah Othman ◽  
Samsuri Abd Wahid ◽  
Fardin Sadegh-Zadeh ◽  
Aminudin Husin
Keyword(s):  
Heavy Metals ◽  
Mine Tailings ◽  
Gold Mine ◽  
Gold Mine Tailings ◽  
Biosorption Of Heavy Metals
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