scholarly journals Reclassification of Brucella ciceri as later heterotypic synonyms of Brucella intermedia

2020 ◽  
Author(s):  
Ayixon Sánchez-Reyes
Keyword(s):  
Type Species ◽  
Single Copy ◽  
Species Level ◽  
New Combinations ◽  
Single Copy Gene ◽  
Phenotypic Data ◽  
Single Clade ◽  
Genomic Divergence ◽  
Copy Gene ◽  
Ochrobactrum Intermedium

AbstractRecently Hördt et al. 2020 proposed to merge Ochrobactrum and Brucella genera based on up to date phylogenomic evidence and overall genomic divergence among Brucella-Ochrobactrum clade. This led to the description of the new combinations Brucella ciceri comb. nov., basonym: Ochrobactrum ciceri Imran et al. 2010 and Brucella intermedia comb. nov., basonym: Ochrobactrum intermedium Velasco et al. 1998. However, the type species for Brucella ciceri DSM 22292T and Brucella intermedia LMG 3301T show whole-genome coherence at the species level (ANI = 98.21 %, Mash D = 0.0154006, dDDH relatedness >70%), suggesting that may belong to the same genomospecies. Also, both taxa formed a single clade in the phylogenomic tree based on single-copy gene sequences. Previously reported phenotypic data offer a context where both taxa are highly related supporting this synonymy. Therefore, Brucella ciceri should be reclassified as later heterotypic synonyms of Brucella intermedia, which has priority. The species description is consequently amended.

scholarly journals Chasing the hare - Evaluating the phylogenetic utility of a nuclear single copy gene region at and below species level within the species rich group Peperomia (Piperaceae)

2011 ◽  
Vol 11 (1) ◽  
pp. 357 ◽  
Author(s):  
Julia Naumann ◽  
Lars Symmank ◽  
Marie-Stéphanie Samain ◽  
Kai F Müller ◽  
Christoph Neinhuis ◽  
...  
Keyword(s):  
Single Copy ◽  
Species Level ◽  
Gene Region ◽  
Single Copy Gene ◽  
Copy Gene

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

A Mouse Single-Copy Gene,Gtf2i,the Homolog of HumanGTF2I,That Is Duplicated in the Williams–Beuren Syndrome Deletion Region

Genomics ◽  
1998 ◽  
Vol 48 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Yu-Ker Wang ◽  
Luis A. Pérez-Jurado ◽  
Uta Francke
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Deletion Region ◽  
Copy Gene

scholarly journals Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents

2021 ◽  
Vol 9 (8) ◽  
pp. 1610
Author(s):  
Christian Klotz ◽  
Elke Radam ◽  
Sebastian Rausch ◽  
Petra Gosten-Heinrich ◽  
Toni Aebischer
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gastrointestinal Disease ◽  
Single Copy ◽  
Marker Genes ◽  
Small Rodent ◽  
Analytical Sensitivity ◽  
Single Copy Gene ◽  
Wild Rodents ◽  
Copy Gene

Giardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for G. duodenalis but are predominantly populated by the two rodent species Giardia microti and Giardia muris. Currently, the detection of zoonotic and non-zoonotic Giardia species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes. This hampers the risk assessment of potential zoonotic Giardia transmissions by these animals. Here, we provide a workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. muris, G. microti and G. duodenalis infections. For the identification of potentially zoonotic G. duodenalis assemblage types A and B, an established protocol targeting the single-copy gene 4E1-HP was used. The assays were specific for the distinct Giardia species or genotypes and revealed an analytical sensitivity of approximately one or below genome equivalent for the multi-copy gene and of about 10 genome equivalents for the single-copy gene. Retesting a biobank of small rodent samples confirmed the specificity. It further identified the underlying Giardia species in four out of 11 samples that could not be typed before by PCR and sequencing. The newly developed workflow has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo

1989 ◽  
Vol 9 (1) ◽  
pp. 329-331
Author(s):  
M Winey ◽  
I Edelman ◽  
M R Culbertson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Analysis ◽  
Single Copy ◽  
Single Copy Gene ◽  
Copy Gene

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

Novel chicken actin gene: third cytoplasmic isoform

1985 ◽  
Vol 5 (5) ◽  
pp. 1151-1162
Author(s):  
D J Bergsma ◽  
K S Chang ◽  
R J Schwartz
Keyword(s):  
Nucleotide Sequence ◽  
Single Copy ◽  
Actin Gene ◽  
Single Copy Gene ◽  
Gene Diversification ◽  
Mrna Transcripts ◽  
Copy Gene ◽  
Actin Mrna ◽  
Distinct Member ◽  
Actin Protein

We identified a novel chicken actin gene. The actin protein deduced from its nucleotide sequence very closely resembles the vertebrate cytoplasmic actins; accordingly, we classified this gene as a nonmuscle type. We adopted the convention for indicating the nonmuscle actins of the class Amphibia (Vandekerckhove et al., J. Mol. Biol. 152:413-426) and denoted this gene as type 5. RNA blot analysis demonstrated that the type 5 actin mRNA transcripts accumulate in adult tissues in a pattern indicative of a nonmuscle actin gene. Genomic DNA blots indicated that the type 5 actin is a single copy gene and a distinct member of the chicken actin multigene family. Inspection of the nucleotide sequence revealed many features that distinguished the type 5 gene from all other vertebrate actin genes examined to date. These unique characteristics include: (i) an initiation Met codon preceding an Ala codon, a feature previously known only in plant actins, (ii) a single intron within the 5' untranslated region, with no interruptions in the coding portion of the gene, and (iii) an atypical Goldberg-Hogness box (ATAGAA) preceding the mRNA initiation terminus. These unusual features have interesting implications for actin gene diversification during evolution.

scholarly journals Intercontinental long-distance dispersal of Canellaceae from the New to the Old World revealed by a nuclear single copy gene and chloroplast loci

2015 ◽  
Vol 84 ◽  
pp. 205-219 ◽  
Author(s):  
Sebastian Müller ◽  
Karsten Salomo ◽  
Jackeline Salazar ◽  
Julia Naumann ◽  
M. Alejandra Jaramillo ◽  
...  
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Long Distance Dispersal ◽  
Long Distance ◽  
Old World ◽  
Copy Gene
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