scholarly journals From qualitative to quantitative insect metabarcoding: an in tandem multilocus mosquito identification methodology

2020 ◽  
Author(s):  
Katerina Kassela ◽  
Adamantia Kouvela ◽  
Michael de Courcy Williams ◽  
Konstantinos Konstantinidis ◽  
Maria Goreti Rosa Freitas ◽  
...  
Keyword(s):  
Large Scale ◽  
Disease Outbreaks ◽  
Small Animal ◽  
Individual Species ◽  
Vector Borne Diseases ◽  
Animal Populations ◽  
Dna Metabarcoding ◽  
Vector Borne ◽  
28S Ribosomal Dna ◽  
Identification And Quantification

AbstractIn the era of emergence and re-emergence of vector-borne diseases, a high throughput trap-based insect monitoring is essential for the identification of invasive species, study of mosquito populations and risk assessment of disease outbreaks. Insect DNA metabarcoding technology has emerged as a highly promising methodology for unbiased and large-scale surveillance. Despite significant attempts to introduce DNA metabarcoding in mosquito or other insect surveillance qualitative and quantitative metabarcoding remains a challenge. In the present study, we have developed a methodology of in-tandem identification and quantification using cytochrome oxidase subunit I (COI) combined with a secondary multilocus identification and quantification involving three loci of 28S ribosomal DNA. The presented methodology was able to identify individual species in pools of mosquitoes with 95.94% accuracy and resolve with high accuracy (p = 1, χ2 = 2.55) mosquito population composition providing a technology capable of revolutionizing mosquito surveillance through metabarcoding. The methodology, given the respective dataset, has the potential to be applied to various small animal populations.

scholarly journals New, Emerging, Re-Emerging Tropical Infectious and Non-Communicable Diseases Persistent to the Climate, Landscape, and Environmental Changes on the Grounds of the Urbanizations, Industrializations, and Globalisation

2021 ◽  
pp. 32-46
Author(s):  
Masimalai Palaniyandi
Keyword(s):  
Infectious Diseases ◽  
Urban Sprawl ◽  
Large Scale ◽  
Urban Landscape ◽  
Communicable Diseases ◽  
Small Scale ◽  
Non Communicable Diseases ◽  
Vector Borne Diseases ◽  
Environmental Transitions ◽  
Vector Borne

Historical records evidenced of urban landscape changes, and environmental transitions brought by the improper growths and urban development’s of the urbanisation and industrialization in the developing countries, especially in India, significantly chaotic urban sprawl and industrial growths, and the development of its allied activities for the recent decades, invites new, emerging, re-emerging, and triggers the tropical infectious diseases including vector borne diseases (VBD) as well non-communicable diseases. Urban sprawl has a multiplier effect of growth of unplanned a crowded housing, and industrialization has an impact on the urban landscape with commercial and market development, and roads over large expanses of urban land while little concern for appropriate urban planning. The union government of India is launching to promote 100 mega smart city projects / metropolitan / urban agglomeration across the nation for betterment of the standard of living infrastructure facilities by 2030. The large scale urban landscape architectural changes, land use / land cover changes, environmental transitions, and micro climatic changes in the heart of the urban landscape, and its fringe areas on the consequence of built-up structures, construction of roads transport networks, drainages, commercial buildings, human dwellings, educational buildings, legal and medical health services, income tax professionals, small scale to large scale industries, etc., The census of India, reports highlighted that people mass movements / migration from rural to the urban, and small towns to mega cities are notably accelerating trends for the recent decades mainly for the purposes of occupation, education, trade and commerce, and professional services, generally reasons for male migration, and marriage is the absolute reason for female adults migration. The spatial and temporal aspects of malaria and dengue has been declining trends in rural settings, however, it has been accelerating trends in the urban settings due to the urban buoyant migrants. Urbanization and industrialization effect on urban landscape environment leads to breakdown of sanitations, water-borne diseases associated with inadequate  and unsafe drinking water supply, tendency to use metal, plastic, and mud pots water storage containers, discarded domestic waste misshapen to vector breeding habitats containers, urban heat island, garbage waste disposal, liquid waste from dwellings, and industries, air pollution (dust, pollen and spores suspended as particles, Sulphur Dioxide-SO2, nitrogen oxides-NO, Carbonate-CO3, depletion of Oxygen O2, Ozone-O3, Methane  Gas- CH₄, Lead- Pb,  Mercury- Hg etc.,), exonerated by the industries and urban transport emissions, modern transport / shipping goods and services, and collectively hazard to human health through erratic infectious diseases and vector borne diseases immediately.

scholarly journals Outbreak of Leptospirosis in Kerala

2018 ◽  
Vol 8 (4) ◽  
pp. 745-747
Author(s):  
Sruthi James ◽  
Brijesh Sathian ◽  
Edwin Van Teijlingen ◽  
Mohammad Asim
Keyword(s):  
Monsoon Rainfall ◽  
Disease Outbreaks ◽  
Farm Animals ◽  
Occupational Hazard ◽  
Vector Borne Diseases ◽  
The North ◽  
Mucous Membranes ◽  
Vector Borne ◽  
Risk Of Exposure ◽  
North And South

In South Asia, the monsoon brings life to vegetation, but at the same time has potential to cause public health problems. Notably, the climate change due to global warming is affecting the extent of monsoon rainfall in the region causing flooding which increases the risks of major disease outbreaks.  Flooding and standing water after heavy rainfall increases the risk of vector-borne diseases such as dengue, malaria, plague, chikungunya, typhoid, cholera and Leptospirosis.  Worldwide, Leptospirosis is one of the most common and emerging zoonoses, except on the North and South Poles. Rat fever or leptospirosis is a bacterial infection caused by the spiral-shaped bacteria (spirochete) of the genus Leptospira. This infection is mainly seen in wild and even domesticated species of rodents. It is mainly transmitted to humans by exposure of the mucous membranes (oral, nasal & eye) and skin abrasions or cuts to the urine or tissues of infected rodents or soil contaminated by their urine. Rats are the primary reservoir of leptospirosis, although farm animals and livestock, such as horses, pigs, dogs or cattle, and even wild animals can also be a reservoir for the bacteria. However, human-to-human transmission seems to occur occasionally. It is also an occupational hazard with potential risk of exposure among outdoors workers such as farmers, cleaners, veterinarians, agricultural workers. Moreover, there exists an increased chance of a recreational hazard to those who swims and wades in contaminated waters .

scholarly journals Leishmaniasis and Climate Change—Case Study: Argentina

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Oscar Daniel Salomón ◽  
María Gabriela Quintana ◽  
Andrea Verónica Mastrángelo ◽  
María Soledad Fernández
Keyword(s):  
Public Health ◽  
Climate Change ◽  
Large Scale ◽  
Local Level ◽  
Point Of View ◽  
Mitigation Strategies ◽  
Vector Borne Diseases ◽  
Scale Models ◽  
Vector Borne

Vector-borne diseases closely associated with the environment, such as leishmaniases, have been a usual argument about the deleterious impact of climate change on public health. From the biological point of view interaction of different variables has different and even conflicting effects on the survival of vectors and the probability transmission of pathogens. The results on ecoepidemiology of leishmaniasis in Argentina related to climate variables at different scales of space and time are presented. These studies showed that the changes in transmission due to change or increase in frequency and intensity of climatic instability were expressed through changes in the probability of vector-human reservoir effective contacts. These changes of contact in turn are modulated by both direct effects on the biology and ecology of the organisms involved, as by perceptions and changes in the behavior of the human communities at risk. Therefore, from the perspective of public health and state policy, and taking into account the current nonlinear increased velocity of climate change, we concluded that discussing the uncertainties of large-scale models will have lower impact than to develop-validate mitigation strategies to be operative at local level, and compatibles with sustainable development, conservation biodiversity, and respect for cultural diversity.

scholarly journals Borrelia infection in rodent host has dramatic effects on the microbiome of ticks

2021 ◽  
Author(s):  
Phineas T. Hamilton ◽  
Elodie Maluenda ◽  
Anouk Sarr ◽  
Alessandro Belli ◽  
Georgia Hurry ◽  
...  
Keyword(s):  
Large Scale ◽  
Vector Competence ◽  
Bacterial Community Composition ◽  
Blood Feeding ◽  
Rodent Host ◽  
Vector Borne Diseases ◽  
Bacterial Microbiome ◽  
Vector Borne ◽  
Scale Experiment ◽  
Tick Microbiome

AbstractBackgroundVector-borne diseases remain major causes of human morbidity and mortality. It is increasingly recognized that the community of microbes inhabiting arthropods can strongly affect their vector competence, but the role of the tick microbiome in Borrelia transmission – the cause of Lyme disease – remains unclear.ResultsHere, we use a large-scale experiment to clarify the reciprocal interactions between Borrelia afzelii and the microbiome of Ixodes ricinus, its primary vector. In contrast to other reports, we find that depletion of the bacterial microbiome in larval ticks has no effect on their subsequent acquisition of B. afzelii during blood feeding on infected mice. Rather, exposure to B. afzelii-infected hosts drives pervasive changes to the tick microbiome, decreasing overall bacterial abundance, shifting bacterial community composition, and increasing bacterial diversity. These effects appear to be independent of the acquisition of B. afzelii by ticks, suggesting they are mediated by physiological or immunological aspects of B. afzelii infection in the rodent host.ConclusionsManipulation of the microbiome of I. ricinus larvae had no effect on their ability to acquire B. afzelii. In contrast, B. afzelii infection in the mouse had dramatic effects on the composition of the gut microbiome in I. ricinus nymphs. Our study demonstrates that vector-borne infections in the vertebrate host shape the microbiome of the arthropod vector.

scholarly journals Critical transitions in malaria transmission models are consistently generated by superinfection

2019 ◽  
Vol 374 (1775) ◽  
pp. 20180275 ◽  
Author(s):  
David Alonso ◽  
Andy Dobson ◽  
Mercedes Pascual
Keyword(s):  
Infectious Disease ◽  
Disease Outbreaks ◽  
Distribution Patterns ◽  
Past Century ◽  
Theme Issue ◽  
Vector Borne Diseases ◽  
Infectious Disease Outbreaks ◽  
Critical Transitions ◽  
Vector Borne ◽  
And Control

The history of modelling vector-borne infections essentially begins with the papers by Ross on malaria. His models assume that the dynamics of malaria can most simply be characterized by two equations that describe the prevalence of malaria in the human and mosquito hosts. This structure has formed the central core of models for malaria and most other vector-borne diseases for the past century, with additions acknowledging important aetiological details. We partially add to this tradition by describing a malaria model that provides for vital dynamics in the vector and the possibility of super-infection in the human host: reinfection of asymptomatic hosts before they have cleared a prior infection. These key features of malaria aetiology create the potential for break points in the prevalence of infected hosts, sudden transitions that seem to characterize malaria’s response to control in different locations. We show that this potential for critical transitions is a general and underappreciated feature of any model for vector-borne diseases with incomplete immunity, including the canonical Ross–McDonald model. Ignoring these details of the host’s immune response to infection can potentially lead to serious misunderstanding in the interpretation of malaria distribution patterns and the design of control schemes for other vector-borne diseases.This article is part of the theme issue ‘Modelling infectious disease outbreaks in humans, animals and plants: approaches and important themes’. This issue is linked with the subsequent theme issue ‘Modelling infectious disease outbreaks in humans, animals and plants: epidemic forecasting and control’.

scholarly journals Relationship between Flooding and Out Break of Infectious Diseasesin Kenya: A Review of the Literature

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Fredrick Okoth Okaka ◽  
Beneah D. O. Odhiambo
Keyword(s):  
Infectious Diseases ◽  
Disease Outbreaks ◽  
Epidemiological Data ◽  
Early Warning Systems ◽  
Mitigation Strategies ◽  
Extensive Literature ◽  
Vector Borne Diseases ◽  
Infectious Disease Outbreaks ◽  
Vector Borne ◽  
The Relationship

Flooding can potentially increase the spread of infectious diseases. To enhance good understanding of the health consequences of flooding and facilitate planning for mitigation strategies, deeper consideration of the relationship between flooding and out-break of infectious diseases is required. This paper examines the relationship between occurrence of floods in Kenya and outbreak of infectious diseases and possible interventions. This review intended to build up the quality and comprehensiveness of evidence on infectious diseases arising after flooding incidence in Kenya. An extensive literature review was conducted in 2017, and published literature from 2000 to 2017 was retrieved. This review suggests that infectious disease outbreaks such as waterborne, rodent-borne, and vector-borne diseases have been associated with flooding in Kenya. But there is need for more good quality epidemiological data to cement the evidence. Comprehensive surveillance and risk assessment, early warning systems, emergency planning, and well-coordinated collaborations are essential in reducing future vulnerability to infectious diseases following flooding.

scholarly journals Focus on Common Small Animal Vector-Borne Diseases in Central and Southeastern Europe

2020 ◽  
Vol 70 (2) ◽  
pp. 147-169
Author(s):  
Leschnik Michael
Keyword(s):  
Small Animal ◽  
Diagnostic Procedures ◽  
Emerging Diseases ◽  
Pathogen Transmission ◽  
Temporal And Spatial Distribution ◽  
Local Climate ◽  
Vector Borne Diseases ◽  
Prophylactic Measures ◽  
Zoonotic Risk ◽  
Vector Borne

AbstractVector-borne diseases are one of the main causes of morbidity and mortality in small animals in Europe. Many of these diseases are well-known among veterinary practitioners and some of them are called emerging diseases as prevalence, temporal and spatial distribution seem to increase in Europe. The number of newly recognized pathogens, transmitted by a variety of arthropod vectors, that are relevant for dogs and cats, is also increasing every year. The prevalence among infected vectors and hosts is a hot topic in veterinary science throughout the entire continent, as well as the development of efficient diagnostic procedures, therapy and prophylactic measures. Companion animal vector-borne diseases comprise a large group of pathogens including viruses, bacteria, protozoa and helminths. These pathogens are mainly transmitted by bloodsucking arthropods (ticks, fleas, mosquitos, sand flies), and more seldom by direct transmission between vertebrate hosts. Vector prevalence and activity is influenced by local climate conditions, host species density, changes in landscape and land use. Human parameters such as poverty and migration affect the use of prophylactic measures against pathogen transmission and infection as well as increasing the zoonotic risk to introducing pathogens by infected humans. Small animal associated factors such as pet trade and pet travel spread infection and certain vectors such as ticks and fleas. All these factors pose several complex and significant challenges for veterinarians in clinical practice to decide on efficient laboratory work-up and constructive diagnostic procedures.

scholarly journals Family Vector Control Response Kit: Equipping Households to Prevent Amplification of Aedes-transmitted Diseases

2021 ◽  
Author(s):  
John H. Thomas ◽  
Nfornuh Alenwi ◽  
Andrew Trevett ◽  
Eric Ochomo ◽  
Timothy Grieve ◽  
...  
Keyword(s):  
Vector Control ◽  
Large Scale ◽  
Disease Outbreaks ◽  
Evidence Base ◽  
Low Literacy ◽  
Or Education ◽  
Vector Borne Diseases ◽  
Emergency Situations ◽  
Comprehensive Education ◽  
Control Response

Abstract BACKGROUNDNatural disasters, such as earthquakes, hurricanes, and floods create ideal mosquito breeding conditions while simultaneously disrupting government services that prevent outbreaks where Aedes-transmitted diseases, and possibly other vector-borne diseases (VBD), are circulating. UNICEF, the MENTOR Initiative, and Kenya Medical Research Institute (KEMRI) evaluated novel UNICEF Family Vector Control Response Kits distributed to households (HH) in Wajir Town, Northeast (NE) Kenya at the end of the rainy season (January/February 2019). This region regularly experiences epidemics of VBD during, and for several months after, rainy seasons. The premise behind this study was to determine if HHs can adopt the use of the kit contents based solely on pictures rather than needing a comprehensive education campaign. This was necessary for the following two reasons: 1. Successful disruption of a disease outbreak often requires transmission be stopped at the HH level as soon as possible, 2. In the beginning of most large-scale rapid response emergencies, it is challenging to enact and align comprehensive education campaigns with delivery of supplies. To stop transmission early, it is often faster, in UNICEF’s experience, to deliver supplies directly to HHs, especially if the global/regional/national supplies are strategically prepositioned in warehouses. The study aimed to determine key outcomes when HHs were given simple picture-based application instructions, without any other education, for different combinations of evidence-based insecticidal products, including adulticides, spatial repellents, larvicides, insecticidal window curtains, and personal protection. The products were those commonly expected to be found in retail markets or will become available through public health procurement channels. METHODSSix different vector control kit configurations were distributed to six randomly selected groups each of 60 HH in Wajir Town. The number of HH successfully used for data collection totalled 324, comprising 60 HH (19%) for direct observation to document behaviors as the kits were opened and contents were being used, and 264 HH (81%) for interviews after using the kit contents. MENTOR measured the uptake, proper application, ability to follow pictorial directions for use, and acceptability at household level. RESULTSEach kit contained a booklet of pictogram Directions for Use (DFU) that described how the product was to be put to practical use, safety precautions, and specific directions where the products should be applied for best performance against Aedes mosquitoes. No further explanation or education was provided. Most interview respondents indicated positively they understood the pictograms for each product. This was an important outcome, given the low literacy level of the responders. The results of the research study provide an operational evidence-base as to the effectiveness of HH managing their own control practices using these kits in a highly insecure and challenging operational setting. CONCLUSIONSThis is the first such study of its kind and has particular relevance adding a new approach to the emergency response capacity for disease control across similar operational settings. The results of the study are relevant wherever there is potential for disease outbreaks caused by Aedes sp and other mosquito vectors in emergency situations.

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