scholarly journals Mapping of Quantitative Trait Locus (QTLs) that contribute to Drought Tolerance in a Recombinant Inbred Line Population of horsegram (Macrotyloma uniflorum)

2021 ◽  
Author(s):  
Megha Katoch ◽  
R.K. Chahota
Keyword(s):  
Drought Stress ◽  
Linkage Group ◽  
Drought Tolerance ◽  
Linkage Map ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Genetic Improvement ◽  
Recombinant Inbred Lines ◽  
Phenotypic Data ◽  
Macrotyloma Uniflorum

AbstractHorsegram (Macrotyloma uniflorum) is a drought hardy legume which can be grown in varied soil and temperature regime. It is an important food legume with environmental, nutritive and medicinal benefits. But in terms of genetic improvement it still lags behind other legumes. To get insight into the genetics of tolerance to drought stress, quantitative trait loci for drought tolerance traits were identified using an intraspecific mapping population comprising of 162 F8 Recombinant Inbred Lines derived from a cross between HPKM249 and HPK4. A total of 2011 markers were screened on parental lines for polymorphism survey, out of which 493 markers were found to be polymorphic and used for genotyping of the RIL population. Of these 493 polymorphic markers, 295 were assigned to ten linkage groups at LOD 3.5 spanning 1541.7cM with a mean distance of 5.20 cM between adjacent markers. This linkage map along with the phenotypic data for drought tolerance traits was used to identify regions of the horsegram genome in which the genes for the qualitative traits linked to drought tolerance located. A total of seven QTLs were identified for six different drought related traits. One QTL for malondialdehyde content on linkage group 2, two QTLs for root length on linkage group 3 & 9, one QTL each for proline content and chlorophyll content under drought stress on linkage group 4, one QTL each for root dry weight and root fresh weight on linkage group 5 were identified using composite interval mapping. The linkage map and identified QTLs will be utilized in Marker Assisted Breeding and increase our understanding on the physiology of drought stress tolerance. It will also aid in molecular breeding efforts for further genetic improvement of horsegram.

Construction of an RAPD linkage map and localization of QTLs for oleic acid level using recombinant inbreds in mustard (Brassica juncea)

Genome ◽  
2002 ◽  
Vol 45 (3) ◽  
pp. 467-472 ◽  
Author(s):  
R Sharma ◽  
R AK Aggarwal ◽  
R Kumar ◽  
T Mohapatra ◽  
R P Sharma
Keyword(s):  
Oleic Acid ◽  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Brassica Juncea ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Acid Level ◽  
Recombinant Inbred Lines ◽  
Phenotypic Variance ◽  
Trait Loci

RAPD markers were employed for construction of a linkage map and localization of QTLs for oleic acid level using a set of 94 recombinant inbred lines (RILs) of mustard (Brassica juncea L.) as a mapping population. Only 30% of the 235 random primers used were useful in terms of polymorphism detected and the reproducibility of those patterns. Normal Mendelian segregation was observed for the majority of the 130 markers obtained with 71 informative primers; only 13.1% deviated (P < 0.01) from the expected 1:1 ratio. One-hundred and fourteen markers were assigned to 21 linkage groups (LGs) covering a total length of 790.4 cM with an average distance of 6.93 cM between markers. Two quantitative trait loci (QTL) for oleic acid level were mapped to 14- and 10.6-cM marker intervals on two different LGs. Both loci together explained 32.2% of phenotypic variance. One major QTL explained 28.5% of the trait variance observed in this species.Key words: Brassica juncea, linkage map, oleic acid, quantitative trait loci, RAPD, recombinant inbred line.

scholarly journals Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders' traits

2018 ◽  
Vol 16 (5) ◽  
pp. 424-436 ◽  
Author(s):  
Carol Moreau ◽  
Maggie Knox ◽  
Lynda Turner ◽  
Tracey Rayner ◽  
Jane Thomas ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Seed Weight ◽  
Genetic Basis ◽  
Recombinant Inbred Lines ◽  
Field Trials ◽  
Genetic Maps ◽  
Genotypic Differences ◽  
Phenotypic Data ◽  
Growing Seasons ◽  
Randomized Field Trials

AbstractIn order to gain an understanding of the genetic basis of traits of interest to breeders, the pea varieties Brutus, Enigma and Kahuna were selected, based on measures of their phenotypic and genotypic differences, for the construction of recombinant inbred populations. Reciprocal crosses were carried out for each of the three pairs, and over 200 F2 seeds from each cross advanced to F13. Bulked F7 seeds were used to generate F8–F11 bulks, which were grown in triplicated plots within randomized field trials and used to collect phenotypic data, including seed weight and yield traits, over a number of growing seasons. Genetic maps were constructed from the F6 generation to support the analysis of qualitative and quantitative traits and have led to the identification of four major genetic loci involved in seed weight determination and at least one major locus responsible for variation in yield. Three of the seed weight loci, at least one of which has not been described previously, were associated with the marrowfat seed phenotype. For some of the loci identified, candidate genes have been identified. The F13 single seed descent lines are available as a germplasm resource for the legume and pulse crop communities.

scholarly journals Molecular-marker-based Genetic Analysis of Tepary Bean-derived Common Bacterial Blight Resistance in Different Developmental Stages of Common Bean

1997 ◽  
Vol 122 (3) ◽  
pp. 329-337 ◽  
Author(s):  
Geunhwa Jung ◽  
Paul W. Skroch ◽  
Dermot P. Coyne ◽  
James Nienhuis ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Linkage Group ◽  
Common Bean ◽  
Linkage Map ◽  
Recombinant Inbred ◽  
Bacterial Blight ◽  
Chromosomal Region ◽  
Bacterial Disease ◽  
Common Bacterial Blight ◽  
Group 5 ◽  
Group 1

Randomly amplified polymorphic DNA (RAPD) molecular markers were used to construct a partial genetic linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross PC-50 × XAN-159 for studying the genetics of bacterial disease resistance in common bean. The linkage map spanned 426 cM and included 168 RAPD markers and 2 classical markers with 11 unassigned markers. The seventy recombinant inbred lines were evaluated for resistance to two strains of common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye] (Xcp). Common bacterial blight (CBB) resistance was evaluated for Xcp strain EK-11 in later-developed trifoliolate leaves and for Xcp strains, DR-7 and EK-11, in first trifoliolate leaves, seeds, and pods. One to four quantitative trait loci (QTLs) accounted for 18% to 53% of the phenotypic variation for traits. Most significant effects for CBB resistance were associated with one chromosomal region on linkage group 5 and with two regions on linkage group 1, of the partial linkage map. The chromosomal region (a 13-cM interval) in linkage group 5 was significantly associated with resistance to Xcp strains DR-7 and EK-11 in leaves, pods, and seeds. The regions in linkage group 1 were also significantly associated with resistance to both Xcp strains in more than one plant organ. In addition, a seedcoat pattern gene (C) and a flower color gene (vlae) were mapped in linkage groups 1 and 5, respectively, of the partial linkage map. The V locus was found to be linked to a QTL with a major effect on CBB resistance.

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