scholarly journals Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders' traits

2018 ◽  
Vol 16 (5) ◽  
pp. 424-436 ◽  
Author(s):  
Carol Moreau ◽  
Maggie Knox ◽  
Lynda Turner ◽  
Tracey Rayner ◽  
Jane Thomas ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Seed Weight ◽  
Genetic Basis ◽  
Recombinant Inbred Lines ◽  
Field Trials ◽  
Genetic Maps ◽  
Genotypic Differences ◽  
Phenotypic Data ◽  
Growing Seasons ◽  
Randomized Field Trials

AbstractIn order to gain an understanding of the genetic basis of traits of interest to breeders, the pea varieties Brutus, Enigma and Kahuna were selected, based on measures of their phenotypic and genotypic differences, for the construction of recombinant inbred populations. Reciprocal crosses were carried out for each of the three pairs, and over 200 F2 seeds from each cross advanced to F13. Bulked F7 seeds were used to generate F8–F11 bulks, which were grown in triplicated plots within randomized field trials and used to collect phenotypic data, including seed weight and yield traits, over a number of growing seasons. Genetic maps were constructed from the F6 generation to support the analysis of qualitative and quantitative traits and have led to the identification of four major genetic loci involved in seed weight determination and at least one major locus responsible for variation in yield. Three of the seed weight loci, at least one of which has not been described previously, were associated with the marrowfat seed phenotype. For some of the loci identified, candidate genes have been identified. The F13 single seed descent lines are available as a germplasm resource for the legume and pulse crop communities.

scholarly journals High-resolution genetic maps ofLotus japonicusandL. burttiibased on re-sequencing of recombinant inbred lines

DNA Research ◽  
2016 ◽  
Vol 23 (5) ◽  
pp. 487-494 ◽  
Author(s):  
Niraj Shah ◽  
Hideki Hirakawa ◽  
Shohei Kusakabe ◽  
Niels Sandal ◽  
Jens Stougaard ◽  
...  
Keyword(s):  
High Resolution ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Genetic Maps

Deploying inter-specific recombinant inbred lines to map QTLs for yield-related traits in soybean

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Yashpal . ◽  
D. R. Rathod ◽  
Subhash Chandra ◽  
Anil Kumar ◽  
Raju Ratan Yadav ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Glycine Soja ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Inbred Lines ◽  
Wild Type ◽  
Single Marker Analysis ◽  
Phenotypic Data ◽  
Single Marker ◽  
Glycine Max L

Quantitative trait loci (QTL) mapping and analyses were conducted for yield and six yield-related traits in soybean using 184 inter-specific recombinant inbred lines (RILs) derived from a cross involving wild type (Glycine soja Sieb. and Zucc.) accession DC2008-1 and cultivated (Glycine max L. Merr.) variety DS9712. A Linkage map of 1639.55 cM length was constructed with 167 SSR markers (65.65% polymorphism) with an average marker interval of 9.82 cM. Using three years phenotypic data 34 QTLs were mapped for 7 traits using Inclusive Composite Interval Mapping approach. The number of QTLs mapped for a trait varied from year to year, however, QTLs for days-to-50% flowering (qDFF5), 100-seed weight (qHSW9-1, qHSW9-2 and qHSW19) and yield (qYLD17) were mapped consistently over the three years of testing. Identified QTLs were validated through single marker analysis in 92 germplasm lines. The study demonstrated the potential of wild type soybean to harness QTLs for yield-related traits. The identified QTLs could be utilized for genetic improvement of soybean through molecular breeding.

scholarly journals Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers.

Genetics ◽  
1995 ◽  
Vol 140 (2) ◽  
pp. 745-754 ◽  
Author(s):  
J Xiao ◽  
J Li ◽  
L Yuan ◽  
S D Tanksley
Keyword(s):  
Molecular Markers ◽  
Qtl Analysis ◽  
Recombinant Inbred ◽  
Quantitative Traits ◽  
Genetic Basis ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Rflp Markers ◽  
Major Contributor ◽  
Significant Qtls

Abstract A set of 194 F7 lines derived from a subspecific rice cross showing strong F1 heterosis was backcrossed to the two parents. The materials (388 BC1F7 lines, 194 F8 lines, two parents, F1) were phenotyped for 12 quantitative traits. A total of 37 significant QTLs (LOD > or = 2.0) was detected through 141 RFLP markers in the BC1F7 populations. Twenty-seven (73%) quantitative trait loci (QTLs) were detected in only one of the BC1F7 populations. In 82% of these cases, the heterozygotes were superior to the respective homozygotes. The remaining 10 (27%) QTLs were detected in both BC1F7 populations, and the heterozygote had a phenotype falling between those of the two homozygotes and in no instances were the heterozygotes found to be superior to both homozygotes. These results suggest that dominance complementation is the major genetic basis of heterosis in rice. This conclusion was strengthened by the finding that there was no correlation between most traits and overall genome heterozygosity and that there were some recombinant inbred lines in the F8 population having phenotypic values superior to the F1 for all of the traits evaluated--a result not expected if overdominance was a major contributor to heterosis. Digenic epistasis was not evident.

Characterization of flowering time response among recombinant inbred lines of WAB638-1/PRIMAVERA rice under reproductive stage drought stress

2021 ◽  
pp. 1-8
Author(s):  
Kehinde A. Adeboye ◽  
Olusegun A. Oduwaye ◽  
Isaac O. Daniel ◽  
Mamadou Fofana ◽  
Mande Semon
Keyword(s):  
Drought Stress ◽  
Flowering Time ◽  
Recombinant Inbred ◽  
Root Length ◽  
Recombinant Inbred Lines ◽  
Block Design ◽  
Field Trials ◽  
Inbred Lines ◽  
Reproductive Stage ◽  
Time Response

Abstract Ninety-eight high-yielding recombinant inbred lines (RILs) of WAB638-1/PRIMAVERA rice were evaluated with the parents under reproductive stage drought stress. The study aimed at characterizing flowering time response under drought stress and consequently classifying the genetic resources for efficient use in breeding programmes. Two field trials were conducted during the 2016 and 2017 dry seasons using the randomized complete block design with two replications. In 2016, 12 RILs were evaluated with the parents for the rooting attributes under drought stress and well-watered treatments. Analysis of variance revealed a significant (P < 0.05) variation among the genotypes for the traits evaluated. Drought stress reduced most of the characters in this study, including grain yield. However, the inbred lines exhibited considerable tolerance to drought stress as indicated by yield-related stress indexes, including stress susceptibility and drought tolerance indexes. Delayed flowering (FD) was recorded in 75 genotypes (including WAB638-1), while 25 genotypes (including PRIMAVERA) had no delay under drought stress compared to the control. The genotypes were grouped into flowering delay (D) genotypes (FD > 1 d) and no delay (N) genotypes (FD < 1 d), which significantly differ (P ⩽ 0.05) for numbers of days to 50% flowering. The flowering delay genotypes may be suitable for intermittent drought, while genotypes with little or no delay may be selected for terminal drought conditions. There was a significant correlation (r > 0.5) between the root length and number of days to 50% flowering. The study indicated that root parameters, such as the root length, may contribute to the drought adaptation mechanisms of the RILs.

scholarly journals Genetic dissection of heterosis of indica–japonica by introgression line, recombinant inbred line and their testcross populations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenqing Yang ◽  
Fan Zhang ◽  
Sundus Zafar ◽  
Junmin Wang ◽  
Huajin Lu ◽  
...  
Keyword(s):  
Grain Yield ◽  
Recombinant Inbred ◽  
Genetic Basis ◽  
Recombinant Inbred Lines ◽  
Introgression Line ◽  
Successful Implementation ◽  
Genetic Dissection ◽  
Single Nucleotide ◽  
Panicle Number ◽  
Main Effect

AbstractThe successful implementation of heterosis in rice has significantly enhanced rice productivity, but the genetic basis of heterosis in rice remains unclear. To understand the genetic basis of heterosis in rice, main-effect and epistatic quantitative trait loci (QTLs) associated with heterosis for grain yield-related traits in the four related rice mapping populations derived from Xiushui09 (XS09) (japonica) and IR2061 (indica), were dissected using single nucleotide polymorphism bin maps and replicated phenotyping experiments under two locations. Most mid-parent heterosis of testcross F1s (TCF1s) of XS09 background introgression lines (XSILs) with Peiai64S were significantly higher than those of TCF1s of recombinant inbred lines (RILs) with PA64S at two locations, suggesting that the effects of heterosis was influenced by the proportion of introgression of IR2061’s genome into XS09 background. A total of 81 main-effect QTLs (M-QTLs) and 41 epistatic QTLs were identified for the phenotypic variations of four traits of RILs and XSILs, TCF1s and absolute mid-parent heterosis in two locations. Furthermore, overdominance and underdominance were detected to play predominant effects on most traits in this study, suggesting overdominance and underdominance as well as epistasis are the main genetic bases of heterosis in rice. Some M-QTLs exhibiting positive overdominance effects such as qPN1.2, qPN1.5 and qPN4.3 for increased panicle number per plant, qGYP9 and qGYP12.1 for increased grain yield per plant, and qTGW3.4 and qTGW8.2 for enhanced 1000-grain weight would be highly valuable for breeding to enhance grain yield of hybrid rice by marker-assisted selection.

scholarly journals Mapping of Quantitative Trait Locus (QTLs) that contribute to Drought Tolerance in a Recombinant Inbred Line Population of horsegram (Macrotyloma uniflorum)

2021 ◽  
Author(s):  
Megha Katoch ◽  
R.K. Chahota
Keyword(s):  
Drought Stress ◽  
Linkage Group ◽  
Drought Tolerance ◽  
Linkage Map ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Genetic Improvement ◽  
Recombinant Inbred Lines ◽  
Phenotypic Data ◽  
Macrotyloma Uniflorum

AbstractHorsegram (Macrotyloma uniflorum) is a drought hardy legume which can be grown in varied soil and temperature regime. It is an important food legume with environmental, nutritive and medicinal benefits. But in terms of genetic improvement it still lags behind other legumes. To get insight into the genetics of tolerance to drought stress, quantitative trait loci for drought tolerance traits were identified using an intraspecific mapping population comprising of 162 F8 Recombinant Inbred Lines derived from a cross between HPKM249 and HPK4. A total of 2011 markers were screened on parental lines for polymorphism survey, out of which 493 markers were found to be polymorphic and used for genotyping of the RIL population. Of these 493 polymorphic markers, 295 were assigned to ten linkage groups at LOD 3.5 spanning 1541.7cM with a mean distance of 5.20 cM between adjacent markers. This linkage map along with the phenotypic data for drought tolerance traits was used to identify regions of the horsegram genome in which the genes for the qualitative traits linked to drought tolerance located. A total of seven QTLs were identified for six different drought related traits. One QTL for malondialdehyde content on linkage group 2, two QTLs for root length on linkage group 3 & 9, one QTL each for proline content and chlorophyll content under drought stress on linkage group 4, one QTL each for root dry weight and root fresh weight on linkage group 5 were identified using composite interval mapping. The linkage map and identified QTLs will be utilized in Marker Assisted Breeding and increase our understanding on the physiology of drought stress tolerance. It will also aid in molecular breeding efforts for further genetic improvement of horsegram.

scholarly journals Identification of Fruit-Associated QTLs in Winter Squash (Cucurbita maxima Duchesne) Using Recombinant Inbred Lines

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 419
Author(s):  
Karolina Kaźmińska ◽  
Ewelina Hallmann ◽  
Aleksandra Korzeniewska ◽  
Katarzyna Niemirowicz-Szczytt ◽  
Grzegorz Bartoszewski
Keyword(s):  
Recombinant Inbred ◽  
Field Experiments ◽  
Recombinant Inbred Lines ◽  
Shape Index ◽  
Inbred Lines ◽  
Matter Content ◽  
Dry Matter Content ◽  
Cucurbita Maxima ◽  
Fresh Market ◽  
Phenotypic Data

Cucurbita maxima Duchesne squash and pumpkins are cultivated world-wide. Cucurbita maxima fruits are produced for fresh market and are valuable for food processing. Therefore, fruit characteristics and yield are the traits of high economic importance for breeders. To date, the genetic basis of fruit-associated traits in C. maxima have been poorly understood. In the present study, we evaluated fruit-associated traits and conducted quantitative trait locus (QTL) analysis using recombinant inbred lines (RILs) derived from a cross of two inbred lines with different fruit morphotypes. Phenotypic data for nine fruit traits (earliness, weight, number per plant, yield per plant, length and diameter, shape index, flesh thickness, sucrose content and dry matter content) were collected for RILs in two open-field experiments. Pairwise analysis of the phenotypic data revealed correlations among the fruit and yield-associated traits. Using a previously developed genetic map, we identified 26 QTLs for eight traits. The QTLs were found in 10 locations on eight chromosomes of C. maxima. The QTLs were detected across experiments and explained up to 41.4% of the observed phenotypic variations. Major-effect QTLs for multiple fruit-associated traits were clustered on chromosome 4, suggesting that this genomic region has been under selection during diversification and/or domestication of C. maxima.

scholarly journals Genetics of Symbiosis in Lotus japonicus: Recombinant Inbred Lines, Comparative Genetic Maps, and Map Position of 35 Symbiotic Loci

2006 ◽  
Vol 19 (1) ◽  
pp. 80-91 ◽  
Author(s):  
Niels Sandal ◽  
Thomas Rørby Petersen ◽  
Jeremy Murray ◽  
Yosuke Umehara ◽  
Bogumil Karas ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Interspecific Cross ◽  
Lotus Japonicus ◽  
Inbred Lines ◽  
Genetic Maps ◽  
Mycorrhizal Symbiosis ◽  
Model Legume ◽  
Common Framework ◽  
Selection Of

Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.

Dissection of the genetic architecture for soybean seed weight across multiple environments

2017 ◽  
Vol 68 (4) ◽  
pp. 358 ◽  
Author(s):  
Weili Teng ◽  
Lei Feng ◽  
Wen Li ◽  
Depeng Wu ◽  
Xue Zhao ◽  
...  
Keyword(s):  
Phenotypic Variation ◽  
Seed Weight ◽  
Average Distance ◽  
Recombinant Inbred Lines ◽  
Molecular Genetic ◽  
Soybean Seed ◽  
Environment Interaction ◽  
Phenotypic Data ◽  
Molecular Genetic Map ◽  
Variation Explained

Seed weight (SW), measured as mass per seed, significantly affects soybean (Glycine max (L.) Merr.) yield and the quality of soybean-derived food. The objective of the present study was to identify quantitative trait loci (QTLs) and epistatic QTLs associated with SW in soybean across 129 recombinant inbred lines (RILs) derived from a cross between Dongnong 46 (100-seed weight, 20.26 g) and ‘L-100 (4.84 g). Phenotypic data were collected from this population after it was grown in nine environments. A molecular genetic map including 213 simple sequence repeat (SSR) markers was constructed, which distributed in 18 of 20 chromosomes (linkage groups). This map encompassed ~3623.39 cM, with an average distance of 17.01 cM between markers. Nine QTLs associated with SW were identified. These QTLs explained 1.07–18.43% of the observed phenotypic variation in the nine different environments, and the phenotypic variation explained by most QTLs was 5–10%. Among these nine QTLs, qSW-3 (Satt192) and qSW-5 (Satt568) explained 2.33–9.96% and 7.26–15.11% of the observed phenotypic variation across eight tested environments, respectively. QTLs qSW-8 (Satt514) and qSW-9 (Satt163) were both identified in six environments and explained 8.99–16.40% and 3.68–18.43% of the observed phenotypic variation, respectively. Nine QTLs had additive and/or additive × environment interaction effects, and the environment-independent QTLs often had higher additive effects. Moreover, nine epistatic pairwise QTLs were identified in different environments. Understanding the existence of additive and epistatic effects of SW QTLs could guide the choice of which reasonable SW QTL to manipulate and could predict the outcomes of assembling a large number of SW QTLs with marker-assisted selection of soybean varieties with desirable SW.

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