scholarly journals Using fecal immunochemical tubes for the analysis of gut microbiome has potential to improve colorectal cancer screening

2021 ◽  
Author(s):  
Kertu Liis Krigul ◽  
Oliver Aasmets ◽  
Kreete Lull ◽  
Tonis Org ◽  
Elin Org
Keyword(s):  
Colorectal Cancer ◽  
Cancer Screening ◽  
Gut Microbiota ◽  
Colorectal Cancer Screening ◽  
Public Health Problem ◽  
Fecal Occult Blood ◽  
Rrna Gene ◽  
Screening Programs ◽  
Fresh Frozen ◽  
The Impact

Background: Colorectal cancer (CRC) is an important and challenging public health problem which successful treatment depends on the early detection of the disease. Recently, colorectal cancer specific microbiome signatures have been proposed as an additional marker for CRC detection. A desirable aim would be the possibility to analyze microbiome from the fecal samples collected during CRC screening programs into FIT tubes for fecal occult blood testing. Methods: We investigated the impact of Fecal Immunohistochemical Test (FIT) and stabilization buffer on the microbial community structure in stool samples from 30 volunteers and compared their communities to fresh-frozen samples highlighting also the previously published cancer-specific communities. Altogether 214 samples were analyzed including positive and negative controls using 16S rRNA gene sequencing. Results: The variation between individuals is greater than differences introduced by collection strategy. The vast majority of the genera are stable up to 7 days. None of the changes observed between fresh frozen samples and FIT tubes are related to previously shown colorectal cancer specific bacteria. Conclusions: Overall, our results show that FIT tubes can be used for profiling the gut microbiota in colorectal cancer screening programs as the community is similar to fresh frozen samples and stable at least for 7 days. Impact: Sample material from FIT tubes could be used in addition to fecal immunochemical tests for future investigations into the role of gut microbiota in colorectal cancer screening programs circumventing the need to collect additional samples and possibly improving the sensitivity of FIT.

scholarly journals Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kertu Liis Krigul ◽  
Oliver Aasmets ◽  
Kreete Lüll ◽  
Tõnis Org ◽  
Elin Org
Keyword(s):  
Colorectal Cancer ◽  
Public Health Problem ◽  
16S Rrna Gene Sequencing ◽  
Occult Blood ◽  
Fecal Occult Blood ◽  
Rrna Gene ◽  
Screening Programs ◽  
Crc Screening ◽  
Fresh Frozen ◽  
The Impact

AbstractColorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker for CRC detection. Since many countries have initiated CRC screening programs, it would be useful to analyze the microbiome in the samples collected in fecal immunochemical test (FIT) tubes for fecal occult blood testing. Therefore, we investigated the impact of FIT tubes and stabilization buffer on the microbial community structure evaluated in stool samples from 30 volunteers and compared the detected communities to those of fresh-frozen samples, highlighting previously published cancer-specific communities. Altogether, 214 samples were analyzed by 16S rRNA gene sequencing, including positive and negative controls. Our results indicated that the variation between individuals was greater than the differences introduced by the collection strategy. The vast majority of the genera were stable for up to 7 days. None of the changes observed between fresh-frozen samples and FIT tube specimens were related to previously identified CRC-specific bacteria. Overall, we show that FIT tubes can be used for profiling the microbiota in CRC screening programs. This circumvents the need to collect additional samples and can possibly improve the sensitivity of CRC detection.

scholarly journals The Colonoscopy Satisfaction and Safety Questionnaire (CSSQP) for Colorectal Cancer Screening: A Development and Validation Study

2019 ◽  
Vol 16 (3) ◽  
pp. 392 ◽  
Author(s):  
Alicia Brotons ◽  
Mercedes Guilabert ◽  
Francisco Lacueva ◽  
José Mira ◽  
Blanca Lumbreras ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Screening ◽  
Colorectal Cancer Screening ◽  
Screening Program ◽  
Occult Blood ◽  
Face Validity ◽  
Screening Colonoscopy ◽  
Fecal Occult Blood ◽  
Optimal Care ◽  
Screening Programs

Colonoscopy services working in colorectal cancer screening programs must perform periodic controls to improve the quality based on patients’ experiences. However, there are no validated instruments in this setting that include the two core dimensions for optimal care: satisfaction and safety. The aim of this study was to design and validate a specific questionnaire for patients undergoing screening colonoscopy after a positive fecal occult blood test, the Colonoscopy Satisfaction and Safety Questionnaire based on patients’ experience (CSSQP). The design included a review of available evidence and used focus groups to identify the relevant dimensions to produce the instrument (content validity). Face validity was analyzed involving 15 patients. Reliability and construct and empirical validity were calculated. Validation involved patients from the colorectal cancer screening program at two referral hospitals in Spain. The CSSQP version 1 consisted of 15 items. The principal components analysis of the satisfaction items isolated three factors with saturation of elements above 0.52 and with high internal consistency and split-half readability: Information, Care, and Service and Facilities features. The analysis of the safety items isolated two factors with element saturations above 0.58: Information Gaps and Safety Incidents. The CSSQP is a new valid and reliable tool for measuring patient’ experiences, including satisfaction and safety perception, after a colorectal cancer screening colonoscopy.

scholarly journals The impact of stratifying by family history in colorectal cancer screening programs

2015 ◽  
Vol 137 (5) ◽  
pp. 1119-1127 ◽  
Author(s):  
Simon Lucas Goede ◽  
Linda Rabeneck ◽  
Iris Lansdorp-Vogelaar ◽  
Ann G. Zauber ◽  
Lawrence F. Paszat ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Screening ◽  
Family History ◽  
Colorectal Cancer Screening ◽  
Screening Programs ◽  
The Impact

scholarly journals The impact of antithrombotics on immunochemical fecal occult blood testing for colorectal cancer screening

2017 ◽  
Vol 28 ◽  
pp. v193
Author(s):  
V. Van der Voort ◽  
L. Wauters ◽  
P. Dobbels ◽  
K. Hendrickx ◽  
V. Casneuf ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Screening ◽  
Colorectal Cancer Screening ◽  
Occult Blood ◽  
Fecal Occult Blood Testing ◽  
Fecal Occult Blood ◽  
Blood Testing ◽  
Fecal Occult ◽  
The Impact

scholarly journals National Colorectal Cancer Screening Program in Lithuania: Description of the 5-Year Performance on Population Level

Cancers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1129
Author(s):  
Audrius Dulskas ◽  
Tomas Poskus ◽  
Inga Kildusiene ◽  
Ausvydas Patasius ◽  
Rokas Stulpinas ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Quality Assurance ◽  
Cancer Screening ◽  
Colorectal Cancer Screening ◽  
Screening Program ◽  
Histopathological Examination ◽  
Insurance Fund ◽  
Crc Screening ◽  
Cancer Screening Program ◽  
The Impact

We aimed to report the results of the implementation of the National Colorectal Cancer (CRC) Screening Program covering all the country. The National Health Insurance Fund (NHIF) reimburses the institutions for performing each service; each procedure within the program has its own administrative code. Information about services provided within the program was retrieved from the database of NHIF starting from the 1 January 2014 to the 31 December 2018. Exact date and type of all provided services, test results, date and results of biopsy and histopathological examination were extracted together with the vital status at the end of follow-up, date of death and date of emigration when applicable for all men and women born between 1935 and 1968. Results were compared with the guidelines of the European Union for quality assurance in CRC screening and diagnosis. The screening uptake was 49.5% (754,061 patients) during study period. Participation rate varied from 16% to 18.1% per year and was higher among women than among men. Proportion of test-positive and test-negative results was similar during all the study period—8.7% and 91.3% annually. Between 9.2% and 13.5% of test-positive patients received a biopsy of which 52.3–61.8% were positive for colorectal adenoma and 4.6–7.3% for colorectal carcinoma. CRC detection rate among test-positive individuals varied between 0.93% and 1.28%. The colorectal cancer screening program in Lithuania coverage must be improved. A screening database is needed to systematically evaluate the impact and performance of the national CRC screening program and quality assurance within the program.

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