scholarly journals The ORGAN SIZE (ORG) locus contributes to isometric gigantism in domesticated tomato

2021 ◽  
Author(s):  
Mateus H Vicente ◽  
Kyle MacLeod ◽  
Cassia RF Figueiredo ◽  
Antonio VDO Figueira ◽  
Fady R Mohareb ◽  
...  
Keyword(s):  
Wild Species ◽  
Tomato Fruit ◽  
Fruit Size ◽  
Leaf Size ◽  
Cell Number ◽  
Reproductive Organ ◽  
Fruit Weight ◽  
Organ Size ◽  
Fine Tuning ◽  
Solanum Pennellii

Gigantism is a key component of the domestication syndrome, a suite of traits that differentiates crops from their wild relatives. Allometric gigantism is strongly marked in horticultural crops, causing disproportionate increases in the size of edible parts such as stems, leaves or fruits. Tomato (Solanum lycopersicum) has attracted attention as a model for fruit gigantism, and many genes have been described controlling this trait. However, the genetic basis of a corresponding increase in size of vegetative organs contributing to isometric gigantism, has remained relatively unexplored. Here, we identified a 0.4 Mbp region on chromosome 7 in introgression lines (ILs) from the wild species Solanum pennellii in two different tomato genetic backgrounds (cv. M82 and cv. Micro-Tom) that controls vegetative and reproductive organ size in tomato. The locus, named ORGAN SIZE (ORG), was fine-mapped using genotype-by-sequencing. A survey of literature revealed that ORG overlaps with previously mapped QTLs controlling tomato fruit weight during domestication. Alleles from the wild species led to reduced cell number in different organs, which was partially compensated by greater cell expansion in leaves but not in fruits. The result was a proportional reduction in leaf, flower and fruit size in the ILs harbouring the wild alleles. These findings suggest that selection for large fruit during domestication also tends to select for increases in leaf size by influencing cell division. Since leaf size is relevant for both source-sink balance and crop adaptation to different environments, the discovery of ORG could allow fine-tuning of these parameters.

SOME EFFECTS ON FRUIT SIZE OF CHROMOSOME 2 OF THE TOMATO

1965 ◽  
Vol 43 (1) ◽  
pp. 137-146
Author(s):  
L. Butler
Keyword(s):  
Linkage Map ◽  
Fruit Size ◽  
Cell Number ◽  
Major Effect ◽  
Fruit Weight ◽  
Pleiotropic Effects ◽  
Chromosome 2 ◽  
Small Fruit ◽  
Number Of Cells

Fruit weights taken from two F2's of 1500 plants indicated that the genes d p o s Lc dil and suf all affect fruit weight. The recessive alleles, except suf and Lc, were associated with small fruit size. The data were analyzed to determine whether this association was the result of linkage or pleiotropic effects. The major effect occurred in the o region, which is some 44 units from the centromere of chromosome 2. The o gene makes the genes oval or pear-shaped instead of spherical, and it is shown that when the locule wall of a spherical fruit and an oval fruit are composed of the same number of cells, the spherical fruit is always heavier. Since cell number is the inherited unit of fruit size, then o is always associated with small size. A gene controlling number of locules, which affects fruit size, is also located in this section of the chromosome. The genes d and s, which are at opposite ends of the present linkage map, both appear to be linked with fruit size genes. It is suggested that these size genes lie in the hetero-chromatin which is adjacent to both ends of the linkage map. The genes dil and suf, which were produced by radiation of the same variety, appear to have pleiotropic effects on fruit size; suf increasing, and dil decreasing fruit size.

scholarly journals Partial Defoliation Can Decrease Average Leaf Size but Has Little Effect on Orange Tree Growth, Fruit Yield and Juice Quality

HortScience ◽  
2005 ◽  
Vol 40 (7) ◽  
pp. 2011-2015 ◽  
Author(s):  
Rongcai Yuan ◽  
Fernando Alferez ◽  
Igor Kostenyuk ◽  
Shila Singh ◽  
James P. Syvertsen ◽  
...  
Keyword(s):  
Leaf Area ◽  
Fruit Size ◽  
Leaf Size ◽  
Fruit Weight ◽  
Fruit Yield ◽  
Soluble Solids ◽  
Juice Quality ◽  
Fruit Number ◽  
Acid Ratio ◽  
Orange Trees

The effects of 2 consecutive years of annual defoliation during the harvest season on fruit size, yield, juice quality, leaf size and number were examined in trees of the midseason cultivar `Hamlin' and the late-season cultivar `Valencia' orange [Citrus sinensis (L.) Osb.]. In `Hamlin', removal of up to 50% of the leaves in late November had no effect on fruit yield, fruit number, fruit size, soluble solids yield, juice °Brix, and °Brix to acid ratio of juice the following year. In `Valencia', removal of 50% of the leaves in late March decreased fruit yield and soluble solids yield but did not affect Brix or the Brix to acid ratio of the juice. Leaf size of new flush was reduced by removal of 50% of the leaves in both cultivars but there was little effect on total canopy size. There were no measured effects of removing 25% of leaves from tree canopies. Thus, canopy growth, fruit yield, fruit quality, and leaf size were not negatively impacted when annual defoliations did not exceed 25% of the total canopy leaf area in `Valencia' and `Hamlin' orange trees for two consecutive years. Overall, fruit weight increased linearly with increasing ratio of leaf area to fruit number, suggesting that fruit enlargement can be limited by leaf area.

scholarly journals Response of Cell Division and Cell Expansion to Local Fruit Heating in Tomato Fruit

2012 ◽  
Vol 137 (5) ◽  
pp. 294-301 ◽  
Author(s):  
Julienne Fanwoua ◽  
Pieter de Visser ◽  
Ep Heuvelink ◽  
Gerco Angenent ◽  
Xinyou Yin ◽  
...  
Keyword(s):  
Cell Expansion ◽  
Tomato Fruit ◽  
Fruit Size ◽  
Growth Period ◽  
Cell Number ◽  
Fruit Growth ◽  
Growth Responses ◽  
Direction Parallel ◽  
Cell Divisions ◽  
Fruit Skin

To improve our understanding of fruit growth responses to temperature, it is important to analyze temperature effects on underlying fruit cellular processes. This study aimed at analyzing the response of tomato (Solanum lycopersicum) fruit size to heating as affected by changes in cell number and cell expansion in different directions. Individual trusses were enclosed into cuvettes and heating was applied either only during the first 7 days after anthesis (DAA), from 7 DAA until fruit maturity (breaker stage), or both. Fruit size and histological characteristics in the pericarp were measured. Heating fruit shortened fruit growth period and reduced final fruit size. Reduction in final fruit size of early-heated fruit was mainly associated with reduction in final pericarp cell volume. Early heating increased the number of cell layers in the pericarp but did not affect the total number of pericarp cells. These results indicate that in the tomato pericarp, periclinal cell divisions respond differently to temperature than anticlinal or randomly oriented cell divisions. Late heating only decreased pericarp thickness significantly. Continuously heating fruit reduced anticlinal cell expansion (direction perpendicular to fruit skin) more than periclinal cell expansion (direction parallel to fruit skin). This study emphasizes the need to measure cell expansion in more than one dimension in histological studies of fruit.

scholarly journals Differences in Cell Number Facilitate Fruit Size Variation in Rabbiteye Blueberry Genotypes

2011 ◽  
Vol 136 (1) ◽  
pp. 10-15 ◽  
Author(s):  
Lisa Klima Johnson ◽  
Anish Malladi ◽  
D. Scott NeSmith
Keyword(s):  
Fruit Development ◽  
Fruit Size ◽  
Size Variation ◽  
Cell Number ◽  
Fruit Weight ◽  
Cell Area ◽  
Vaccinium Ashei ◽  
Cell Production ◽  
University Of Georgia ◽  
Fruit Diameter

Fruit size is a valuable commercial trait in blueberry. The cellular basis of variation in fruit size among rabbiteye blueberry (Vaccinium ashei) genotypes was investigated. Twenty genotypes, including cultivars and advanced selections from the University of Georgia blueberry breeding program, were analyzed. Among the 20 genotypes, fruit weight and fruit diameter varied by over threefold and 1.6-fold, respectively. Regression analysis indicated a linear relationship between fruit weight and fruit diameter (R2 = 0.97, P < 0.001), suggesting that fruit diameter is a good predictor of fruit weight. Among the 20 genotypes, mesocarp cell number and cell area varied by almost 2.5-fold and 1.5-fold, respectively. Although fruit diameter and cell number were significantly related (R2 = 0.79, P < 0.001), no relationship could be established between fruit diameter and cell area. These data indicate that variation in fruit size among rabbiteye blueberry genotypes is primarily facilitated by variation in cell number. Two small and two large fruit size genotypes were further analyzed. Differences in cell number among some of these genotypes were apparent at bloom suggesting that cell production before bloom is an important mechanism contributing to variation in final cell number. Differences in final cell number among other genotypes were manifested during fruit development, indicating that cell production during fruit development was also instrumental in determining variation in final cell number. This study suggests that fruit size variation in rabbiteye blueberry genotypes is determined by mechanisms that regulate cell production before bloom and during fruit development.

scholarly journals Dissecting the Genetic Pathway to Extreme Fruit Size in Tomato Using a Cross Between the Small-Fruited Wild Species Lycopersicon pimpinellifolium and L. esculentum var. Giant Heirloom

Genetics ◽  
2001 ◽  
Vol 158 (1) ◽  
pp. 413-422 ◽  
Author(s):  
Zachary Lippman ◽  
Steven D Tanksley
Keyword(s):  
Qtl Analysis ◽  
Wild Species ◽  
Fruit Size ◽  
Fruit Weight ◽  
Chromosome 2 ◽  
Chromosome 11 ◽  
Lycopersicon Pimpinellifolium ◽  
Carpel Number ◽  
Locule Number ◽  
First Time

Abstract In an effort to determine the genetic basis of exceptionally large tomato fruits, QTL analysis was performed on a population derived from a cross between the wild species Lycopersicon pimpinellifolium (average fruit weight, 1 g) and the L. esculentum cultivar var. Giant Heirloom, which bears fruit in excess of 1000 g. QTL analysis revealed that the majority (67%) of phenotypic variation in fruit size could be attributed to six major loci localized on chromosomes 1-3 and 11. None of the QTL map to novel regions of the genome—all have been reported in previous studies involving moderately sized tomatoes. This result suggests that no major QTL beyond those already reported were involved in the evolution of extremely large fruit. However, this is the first time that all six QTL have emerged in a single population, suggesting that exceptionally large-fruited varieties, such as Giant Heirloom, are the result of a novel combination of preexisting QTL alleles. One of the detected QTL, fw2.2, has been cloned and exerts its effect on fruit size through global control of cell division early in carpel/fruit development. However, the most significant QTL detected in this study (fw11.3, lcn11.1) maps to the bottom of chromosome 11 and seems to exert its effect on fruit size through control of carpel/locule number. A second major locus, also affecting carpel number (and hence fruit size), was mapped to chromosome 2 (fw2.1, lcn2.1). We propose that these two carpel number QTL correspond to the loci described by early classical geneticists as fasciated (f) and locule number (lc), respectively.

scholarly journals 683 Contributions of Early Season Environment and Crop Load to Apple Fruit Development

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 516D-516
Author(s):  
C.J. Stanley ◽  
D.S. Tustin
Keyword(s):  
Cell Division ◽  
Fruit Size ◽  
Cell Number ◽  
Apple Fruit ◽  
Fruit Weight ◽  
Full Bloom ◽  
Crop Load ◽  
Early Season ◽  
Growth Partitioning ◽  
Load Conditions

Many factors contribute to final apple fruit size. Researchers have studied these factors and have developed models, some very complex. Results from many New Zealand regions over several years suggest that early season temperature along with crop load are the key factors driving final fruit size. Accumulated growing degree days from full bloom to 50 days after full bloom (DAFB), accounted for 90% of the variance in fruit weight of `Royal Gala' apples at 50 DAFB under nonlimiting low-crop-load conditions. In turn, fruit weight at 50 DAFB accounted for 90% of the variance in final fruit size at harvest under the low-crop-load conditions. We hypothesise that a potential maximum fruit size is set by 50 DAFB, determined by total fruit cell number, resulting from a temperature-responsive cell division phase. Under conditions of no limitations after the cell division phase, we suggest that all cells would expand to their optimum size to provide the maximum fruit size achievable for that cell number. Factors which affect growth partitioning among fruits, e.g., higher crop loads, would reduce final fruit size, for any given cell number, when grown in the same environment. In Oct. 1999, four different crop loads were established at full bloom on `Royal Gala' trees (M9 rootstock) in four climatically different regions. In Hawkes Bay, similar crop loads were established at 50 DAFB on additional trees. Hourly temperatures were recorded over the season. Fruit size was measured at 50 DAFB and fruit will be harvested in Feb. 2000. These data should provide fresh insight and discussion into the respective roles of temperature and competition during the cell division fruit growth phase on apple fruit size.

Histological and molecular investigation of the basis for variation in tomato fruit size in response to fruit load and genotype

2012 ◽  
Vol 39 (9) ◽  
pp. 754 ◽  
Author(s):  
Julienne Fanwoua ◽  
Pieter H. B. de Visser ◽  
Ep Heuvelink ◽  
Gerco Angenent ◽  
Xinyou Yin ◽  
...  
Keyword(s):  
Cell Volume ◽  
Molecular Mechanisms ◽  
Tomato Fruit ◽  
Sugar Content ◽  
Genotypic Variation ◽  
Fruit Size ◽  
Cell Number ◽  
Parental Line ◽  
Genotypic Differences ◽  
Solanum Lycopersicum L

Understanding the molecular mechanisms and cellular dynamics that cause variation in fruit size is critical for the control of fruit growth. The aim of this study was to investigate how both genotypic factors and carbohydrate limitation cause variation in fruit size. We grew a parental line (Solanum lycopersicum L.) and two inbred lines from Solanum chmielewskii (C.M.Rick et al.; D.M.Spooner et al.) producing small or large fruits under three fruit loads (FL): continuously two fruits/truss (2&2F) or five fruits/truss (5&5F) and a switch from five to two fruits/truss (5&2F) 7 days after anthesis (DAA). Final fruit size, sugar content and cell phenotypes were measured. The expression of major cell cycle genes 7 DAA was investigated using quantitative PCR. The 5&5F treatment resulted in significantly smaller fruits than the 5&2F and 2&2F treatments. In the 5&5F treatment, cell number and cell volume contributed equally to the genotypic variation in final fruit size. In the 5&2F and 2&2F treatment, cell number contributed twice as much to the genotypic variation in final fruit size than cell volume did. FL treatments resulted in only subtle variations in gene expression. Genotypic differences were detected in transcript levels of CycD3 (cyclin) and CDKB1 (cyclin-dependent-kinase), but not CycB2. Genotypic variation in fruit FW, pericarp volume and cell volume was linked to pericarp glucose and fructose content (R2 = 0.41, R2 = 0.48, R2 = 0.11 respectively). Genotypic variation in cell number was positively correlated with pericarp fructose content (R2 = 0.28). These results emphasise the role of sugar content and of the timing of assimilate supply in the variation of cell and fruit phenotypes.

scholarly journals Associations among Morphological and Phonological Characters Representing Apricot Germplasm in Central Mexico

1992 ◽  
Vol 117 (3) ◽  
pp. 486-490 ◽  
Author(s):  
S. Perez-Gonzales
Keyword(s):  
Growth Habit ◽  
Fruit Size ◽  
Leaf Size ◽  
Fruit Weight ◽  
Prunus Armeniaca ◽  
Yield Potential ◽  
Breeding Programs ◽  
Local Conditions ◽  
Basal Diameter ◽  
Factors Associated

Twenty variables were recorded on 15 apricot (Prunus armeniaca L.) genotypes differing in growth habit and blossom time to detect possible associations among morphological and phonological traits. The widest range of variability observed among phenotypes was for fruit size and factors associated with adaptation to local conditions, such as blossom season and yield potential as expressed by number of buds, flowers, and fruits per length of fruiting spurs. The most important morphological traits correlated with fruit weight were tree growth habit, apical and basal diameter of fruiting spurs, and bud and leaf size. Multivariate analysis allowed tree and variable grouping, which might encompass the basic criteria for apricot breeding programs in central México.

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