scholarly journals Genome assemblies of three closely related leaf beetle species (Galerucella spp).

2021 ◽  
Author(s):  
Xuyue Yang ◽  
Tanja Slotte ◽  
Peter A. Hambäck
Keyword(s):  
Population Genomics ◽  
Biological Control Agent ◽  
Leaf Beetle ◽  
Control Agent ◽  
Beetle Species ◽  
Protein Coding ◽  
A Genome ◽  
Genome Assemblies ◽  
Evolutionary Comparisons ◽  
Future Population

Galerucella (Coleoptera: Chrysomelidae) is a leaf beetle genus that has been extensively used for ecological and evolutionary studies. It has also been used as biological control agent against invading purple loosestrife in North America, with large effects on biodiversity. Here we report genome assembly and annotation of three closely related Galerucella species: G. calmariensis, G. pusilla and G. tenella. The three assemblies have a genome size ranging from 460Mb to 588Mb, with N50 from 31,588kb to 79.674kb, containing 29,202 to 40,929 scaffolds. Using an ab initio evidence-driven approach, 30,302 to 33,794 protein-coding genes were identified and functionally annotated. These draft genomes will contribute to the understanding of host-parasitoid interactions, evolutionary comparisons of leaf beetle species and future population genomics studies.

scholarly journals Genome assemblies of three closely related leaf beetle species (Galerucella spp)

2021 ◽  
Author(s):  
Xuyue Yang ◽  
Tanja Slotte ◽  
Jacques Dainat ◽  
Peter A Hambäck
Keyword(s):  
Population Genomics ◽  
Biological Control Agent ◽  
Leaf Beetle ◽  
Control Agent ◽  
Beetle Species ◽  
Protein Coding ◽  
A Genome ◽  
Genome Assemblies ◽  
Evolutionary Comparisons ◽  
Future Population

Abstract Galerucella (Coleoptera: Chrysomelidae) is a leaf beetle genus that has been extensively used for ecological and evolutionary studies. It has also been used as biological control agent against invading purple loosestrife in North America, with large effects on biodiversity. Here we report genome assembly and annotation of three closely related Galerucella species: G. calmariensis, G. pusilla and G. tenella. The three assemblies have a genome size ranging from 460 Mbp to 588 Mbp, with N50 from 31,588 kbp to 79.674 kbp, containing 29,202 to 40,929 scaffolds. Using an ab initio evidence-driven approach, 30,302 to 33,794 protein-coding genes were identified and functionally annotated. These draft genomes will contribute to the understanding of host-parasitoid interactions, evolutionary comparisons of leaf beetle species and future population genomics studies.

scholarly journals Mitochondrial Genome Sequences of Diorhabda carinata and Diorhabda carinulata, Two Beetle Species Introduced to North America for Biological Control

2019 ◽  
Vol 8 (35) ◽  
Author(s):  
A. R. Stahlke ◽  
A. Z. Ozsoy ◽  
D. W. Bean ◽  
P. A. Hohenlohe
Keyword(s):  
Biological Control ◽  
North America ◽  
Mitochondrial Genome ◽  
Noncoding Region ◽  
Beetle Species ◽  
Genome Sequences ◽  
Protein Coding ◽  
Content Type ◽  
Protein Coding Genes ◽  
Genome Assemblies

We announce the complete circularized mitochondrial genome assemblies of Diorhabda carinata and Diorhabda carinulata, beetle species introduced to North America for the biological control of invasive shrubs of the genus Tamarix L. (Tamaricaceae). The assemblies (16,232 and 16,298 bp, respectively) each comprise 13 protein-coding genes, 22 tRNAs, two rRNAs, and a noncoding region.

scholarly journals Hybrid Genome Assembly and Evidence-Based Annotation of the Egg Parasitoid and Biological Control Agent Trichogramma brassicae

2020 ◽  
Vol 10 (10) ◽  
pp. 3533-3540 ◽  
Author(s):  
Kim B. Ferguson ◽  
Tore Kursch-Metz ◽  
Eveline C. Verhulst ◽  
Bart A. Pannebakker
Keyword(s):  
Biological Control ◽  
De Novo ◽  
Biological Control Agent ◽  
Egg Parasitoid ◽  
Control Agent ◽  
Evidence Based ◽  
Trichogramma Brassicae ◽  
A Genome ◽  
Hybrid Genome ◽  
Essential Resource

Trichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and sequenced in a hybrid de novo strategy, after which several assembling strategies were evaluated. The best assembly, derived from a DBG2OLC-based pipeline, yielded a genome of 235 Mbp made up of 1,572 contigs with an N50 of 556,663 bp. Following a rigorous ab initio-, homology-, and evidence-based annotation, 16,905 genes were annotated and functionally described. As an example of the utility of the genome, a simple ortholog cluster analysis was performed with sister species T. pretiosum, revealing over 6000 shared clusters and under 400 clusters unique to each species. The genome and transcriptome presented here provides an essential resource for comparative genomics of the commercially relevant genus Trichogramma, but also for research into molecular evolution, ecology, and breeding of T. brassicae.

scholarly journals Hybrid genome assembly and evidence-based annotation of the egg parasitoid and biological control agent Trichogramma brassicae

2020 ◽  
Author(s):  
K. B. Ferguson ◽  
T. Kursch-Metz ◽  
E. C. Verhulst ◽  
B. A. Pannebakker
Keyword(s):  
Biological Control ◽  
De Novo ◽  
Biological Control Agent ◽  
Egg Parasitoid ◽  
Control Agent ◽  
Evidence Based ◽  
Trichogramma Brassicae ◽  
A Genome ◽  
Hybrid Genome ◽  
Essential Resource

ABSTRACTTrichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and sequenced in a hybrid de novo strategy, after which several assembling strategies were evaluated. The best assembly, derived from a DBG2OLC-based pipeline, yielded a genome of 235 Mbp made up of 1,572 contigs with an N50 of 556,663 bp. Following a rigorous ab initio-, homology-, and evidence-based annotation, 16,905 genes were annotated and functionally described. As an example of the utility of the genome, a simple ortholog cluster analysis was performed with sister species T. pretiosum, revealing over 6000 shared clusters and under 400 clusters unique to each species. The genome and transcriptome presented here provides an essential resource for comparative genomics of the commercially relevant genus Trichogramma, but also for research into molecular evolution, ecology, and breeding of T. brassicae.

scholarly journals Complete Genome Sequence Data of the Grapevine Crown Gall inhibiting bacteria Allorhizobium vitis Strain F2/5.

2021 ◽  
Author(s):  
Hangwei Xi ◽  
Joshua Grist ◽  
Maarten Ryder ◽  
Iain Searle
Keyword(s):  
Genome Sequence ◽  
Crown Gall ◽  
Sequence Data ◽  
Biological Control Agent ◽  
Control Agent ◽  
Protein Coding ◽  
Crown Gall Disease ◽  
Allorhizobium Vitis ◽  
Grapevine Crown Gall ◽  
Circular Chromosomes

Crown gall disease in grapevine is caused by pathogenic strains of Allorhizobium vitis. A. vitis strain F2/5 is a non-pathogenic biocontrol agent that was previously shown to act as a biological control agent to crown gall disease and first isolated from South Africa. Here, we present the complete assembled genome and is 5.94 Mb in length with 5,414 predicted protein-coding sequences, has two circular chromosomes and five plasmids. The genome sequence has no detectable T-DNA border sequences and is missing key virulence genes which is consistent with the bacteria being non-pathogenic. The F2/5 genome sequence could contribute to understanding the molecular basis underlying the biocontrol activity.

scholarly journals Neolema ogloblini exploring a new option for the control of tradescantia (Tradescantia fluminensis)

2015 ◽  
Vol 68 ◽  
pp. 187-192
Author(s):  
S.D. Jackman ◽  
P.G. Peterson ◽  
A.W. Robertson ◽  
C. Van_Koten
Keyword(s):  
Biological Control Agent ◽  
Seedling Survival ◽  
Survival Rates ◽  
Leaf Beetle ◽  
Control Agent ◽  
Growth And Survival ◽  
Light Levels ◽  
Warm Temperate Forest ◽  
Tradescantia Fluminensis ◽  
Environmental Weed

The leaf beetle Neolema ogloblini was released in 2011 as a biological control agent for Tradescantia fluminensis a major warm temperate forest environmental weed in New Zealand To assess whether N ogloblini can suppress T fluminensis and improve native seedling growth and survival a glasshouse experiment was established Kawakawa (Macropiper excelsum) and mahoe (Melicytus ramiflorus) seedlings were planted underneath uncontrolled T fluminensis and compared with seedlings (1) under T fluminensis damaged by N ogloblini (2) under T fluminensis sprayed with herbicide (triclopyr) and (3) released from competition by manually removing T fluminensis Seedlings did not grow faster in response to reduced T fluminensis biomass and increased light levels following feeding by N ogloblini over the 12 week experiment However seedling survival rates were higher (kawakawa 87 and mahoe 93) with N ogloblini feeding than herbicidetreatment (kawakawa 17 and mahoe 3) T fluminensis Survival in uncontrolled T fluminensis (kawakawa 90 and mahoe 57) varied for the two species This experiment suggests that regeneration of native plants may benefit from damage to T fluminensis caused by N ogloblini feeding in the field

scholarly journals Erratum to: Assessment of potential non-target effects of Tetrastichus julis, a biological control agent of the cereal leaf beetle, Oulema melanopus

BioControl ◽  
2016 ◽  
Vol 61 (4) ◽  
pp. 413-413
Author(s):  
Vincent A. D. Hervet ◽  
Héctor A. Cárcamo ◽  
Lloyd M. Dosdall ◽  
Terry D. Miller ◽  
Swaroop V. Kher
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Leaf Beetle ◽  
Control Agent ◽  
Cereal Leaf Beetle ◽  
Oulema Melanopus ◽  
Target Effects
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