scholarly journals A Comparison of Four Commercially Available RNA Extraction Kits for Wastewater Surveillance of SARS-CoV-2 in a College Population

2021 ◽  
Author(s):  
Megan O'Brian ◽  
Zachary C Rundell ◽  
Michelle D Nemec ◽  
Laura M Langan ◽  
Jeffrey A Back ◽  
...  
Keyword(s):  
Public Health ◽  
New England ◽  
Rna Extraction ◽  
University Campus ◽  
Population Densities ◽  
Soil Microbe ◽  
Processing Times ◽  
College Population ◽  
Public Health Officials ◽  
Wastewater Samples

Localized wastewater surveillance has allowed for public health officials to gain a broader understanding of SARS-CoV-2 viral prevalence in the community allowing public health officials time to prepare for impending outbreaks. Given variable levels of virus in the population through public health interventions, proper concentration and extraction of viral RNA is a key step in ensuring accurate detections. With many commercial RNA extraction kits and methodologies available, the performance of 4 different kits were evaluated for SARS-CoV-2 RNA detection in wastewater, specifically focusing on their applicability to lower population densities such as those at university campus dorms. Raw wastewater samples were collected at 4 sites on a college campus over a 24 hour period as a composite sample. Included in these sites was an isolation site that housed students that tested positive for Covid-19 via nasopharyngeal swabs. These samples were analyzed using the following kits: Qiagen All Prep PowerViral DNA/RNA kit, New England BioLabs Monarch RNA MiniPrep Kit, and Zymo Quick RNA-Viral Kit, and the Zymo Quick-RNA Fecal/Soil Microbe MicroPrep Kit. All four sites were processed according to the manufacturers guidelines. Extractions were then quantified with RT-qPCR one-step reactions using an N2 primer and a linearized plasmid standard. While the Zymo Quick-RNA Fecal/Soil Microbe MicroPrep Kit (also known as the Zymo Environ Water RNA Kit) only recovered approximately 73% (+/- 38%) SARS-CoV-2 RNA compared to the Zymo Quick-RNA Viral kit, it was the most time efficient kit to yield comparable results. This extraction kit had a cumulative processing time of approximately five hours compared, while the other three kits had processing times between approximately 9 and 9.5 hours. Based on the current research, the most effective kits for smaller population densities are pellet based and include a homogenization, inhibitor removal, and RNA preservation step.

Cooperation, Conflict, and Reaction

2001 ◽  
Author(s):  
John T. Cumbler
Keyword(s):  
Public Health ◽  
New England ◽  
The Other ◽  
Potential Conflict ◽  
Stream Pollution ◽  
The Public ◽  
Public Health Officials ◽  
The One ◽  
The Right

When James Olcott spoke before Connecticut farmers for “anti-stream pollution,” he urged the public to mobilize to stop water pollution by “ignorant or reckless capitalists.” In identifying the “ignorant and reckless capitalists,” Olcott focused the attention of the farmers on industrial waste and the role of manufacturers in their search for profits in causing pollution. Although manufacturers and the courts argued that industrialization brought wealth and prosperity to New England and hence was a general good, Olcott challenged this idea. He saw the issue as a conflict between industrialization and its costs on the one hand and the public good on the other. Concern over industrial pollution and the potential conflict between it and public health had already arisen in Massachusetts. Although the Massachusetts State Board of Health realized that the interests of the “capitalists” and those of the public health officials might be in conflict, in 1872 it hoped that with improved knowledge, “a way will be eventually found to joining them into harmonious relations,” much as Lyman believed science and technology would resolve the conflict between fishers and mill owners. The board's interest in “harmonious relations” also reflected a realization that at least for the last several years, the courts had seen pollution as an inevitable consequence of civilization and had been favorable toward industrialists, especially if no obvious alternative to dumping pollution existed. In 1866, William Merrifield sued Nathan Lombard because Lombard had dumped “Vitriol and other noxious substances” into the stream above Merrifield's factory, “corrupting” the water so badly that it destroyed his boiler. Chief Justice Bigelow ruled that Lombard had invaded Merrifield's rights. “Each riparian owner,” the judge wrote, “has the right to use the water for any reasonable and proper purpose. . . . An injury to the purity or quality of the water to the detriment of the other riparian owners, constitutes in legal effect, a wrong.” In 1872, Merrifield again went to court, claiming the City of Worcester regularly dumped sewage into Mill Brook, by which the waters became greatly corrupted and unfit to use.”

Perspectives on Prevention: A Dialogue Between Public Health Officials and Community Psychologists

2002 ◽  
Author(s):  
Steven B. Pokorny ◽  
Peter Y. Ji ◽  
Jospeh L. Sherk ◽  
P. Jacob Rebus ◽  
Olga Rabin-Belyaev ◽  
...  
Keyword(s):  
Public Health ◽  
Public Health Officials

Dealing with COVID-19: lessons learned from the Italian experience

Coronaviruses ◽  
2020 ◽  
Vol 01 ◽  
Author(s):  
Silvana Mirella Aliberti ◽  
Francesco De Caro ◽  
Giovanni Boccia ◽  
Rosario Caruso ◽  
Mario Capunzo
Keyword(s):  
Public Health ◽  
Risk Assessment ◽  
Lessons Learned ◽  
Coherent System ◽  
Effective Response ◽  
Medical Practitioners ◽  
Italian Experience ◽  
Rapid Spread ◽  
Public Health Officials ◽  
Future Plans

: Italy was the first western nation affected by the pandemic and was observed as a pilot case in the management of the new coronavirus epidemic. The outbreak of COVID-19 disease has been very difficult in Italy, on June 25, 2020 there are 239,821 total cases of which 33,592 deaths nationwide. Three lessons emerged from this experience that can serve as a blueprint to improve future plans for the outbreak of viruses. First, early reports on the spread of COVID-19 can help inform public health officials and medical practitioners in effort to combat its progression; second, inadequate risk assessment related to the urgency of the situation and limited reporting to the virus has led the rapid spread of COVID-19; third, an effective response to the virus had to be undertaken with coherent system of actions and simultaneously.

Communications and Politics

2018 ◽  
Author(s):  
Joshua M. Sharfstein
Keyword(s):  
Public Health ◽  
Disease Control ◽  
Accurate Information ◽  
Political Judgment ◽  
Political Leaders ◽  
Health Crisis ◽  
Public Health Crisis ◽  
Control And Prevention ◽  
Public Health Officials ◽  
Centers For Disease Control

An effective communications approach starts with a basic dictum set forth by the Centers for Disease Control and Prevention: “Be first, be right, be credible.” Agencies must establish themselves as vital sources of accurate information to maintain the public’s trust. At the same time, public health officials must recognize that communications play out in the context of ideological debates, electoral rivalries, and other political considerations. During a public health crisis, this means that health officials often need to constructively engage political leaders in communications and management. Navigating these waters in the middle of a crisis can be treacherous. Figuring out the best way to engage elected leaders is a core aspect of political judgment.

Postexposure Interventions to Prevent Infection With HBV, HCV, or HIV, and Tetanus in People Wounded During Bombings and Other Mass Casualty Events—United States, 2008: Recommendations of the Centers for Disease Control and Prevention and Disaster Medicine and Public Health Preparedness

2008 ◽  
Vol 2 (3) ◽  
pp. 150-165 ◽  
Author(s):  
Louisa E. Chapman ◽  
Ernest E. Sullivent ◽  
Lisa A. Grohskopf ◽  
Elise M. Beltrami ◽  
Joseph F. Perz ◽  
...  
Keyword(s):  
Public Health ◽  
Disease Control ◽  
Emergency Response ◽  
Mass Casualty ◽  
Acute Injury ◽  
Mass Casualties ◽  
Public Health Preparedness ◽  
Mass Casualty Event ◽  
Public Health Officials ◽  
Centers For Disease Control

ABSTRACTPeople wounded during bombings or other events resulting in mass casualties or in conjunction with the resulting emergency response may be exposed to blood, body fluids, or tissue from other injured people and thus be at risk for bloodborne infections such as hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or tetanus. This report adapts existing general recommendations on the use of immunization and postexposure prophylaxis for tetanus and for occupational and nonoccupational exposures to bloodborne pathogens to the specific situation of a mass casualty event. Decisions regarding the implementation of prophylaxis are complex, and drawing parallels from existing guidelines is difficult. For any prophylactic intervention to be implemented effectively, guidance must be simple, straightforward, and logistically undemanding. Critical review during development of this guidance was provided by representatives of the National Association of County and City Health Officials, the Council of State and Territorial Epidemiologists, and representatives of the acute injury care, trauma, and emergency response medical communities participating in the Centers for Disease Control and Prevention’s Terrorism Injuries: Information, Dissemination and Exchange project. The recommendations contained in this report represent the consensus of US federal public health officials and reflect the experience and input of public health officials at all levels of government and the acute injury response community. (Disaster Med Public Health Preparedness. 2008;2:150–165)

Unresolved Issues in Controlling the Tuberculosis Epidemic Among the Foreign-Born in the United States

1996 ◽  
Vol 22 (4) ◽  
pp. 503-536
Author(s):  
Guido S. Weber
Keyword(s):  
Public Health ◽  
The United States ◽  
Laboratory Research ◽  
Treatment Programs ◽  
State Governments ◽  
Foreign Born ◽  
Health Crisis ◽  
Department Of Health ◽  
Public Health Officials ◽  
Year 2000

Tuberculosis (TB), “the world’s most neglected health crisis,” has returned after decades of decline, but has only gradually caught the attention of governments as a formidable threat to public health. By 1984, when TB cases hit an all-time low, federal and state governments stopped supporting the medical infrastructure that once served to contain the disease. State officials around the nation began dismantling laboratory research programs and closing TB clinics and sanitoria. Since 1985, however, TB rates have steadily increased to 26,673 reported cases in 1992, and some have estimated that by the year 2000, there could be a twenty percent increase. By 1993, Congress, realizing that TB could pose a major public health threat, allocated over $100 million to the Department of Health and Human Services for TB prevention and treatment programs. Those funds, however, were sorely needed years before and amounted to only a fraction of what public health officials believe necessary to control TB today.

scholarly journals Willingness of Greek general population to get a COVID-19 vaccine

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Georgia Kourlaba ◽  
Eleni Kourkouni ◽  
Stefania Maistreli ◽  
Christina-Grammatiki Tsopela ◽  
Nafsika-Maria Molocha ◽  
...  
Keyword(s):  
Public Health ◽  
General Population ◽  
Data Collection ◽  
Sample Size ◽  
Significant Proportion ◽  
Control Measures ◽  
Systematic Sampling ◽  
Computer Assisted ◽  
Public Health Officials ◽  
To Receive

Abstract Background Epidemiological data indicate that a large part of population needs to be vaccinated to achieve herd immunity. Hence, it is of high importance for public health officials to know whether people are going to get vaccinated for COVID-19. The objective of the present study was to examine the willingness of adult residents in Greece to receive a COVID-19 vaccine. Methods A cross-sectional was survey conducted among the adult general population of Greece between April 28, 2020 to May 03, 2020 (last week of lockdown), using a mixed methodology for data collection: Computer Assisted Telephone Interviewing (CATI) and Computer Assisted web Interviewing (CAWI). Using a sample size calculator, the target sample size was found to be around 1000 respondents. To ensure a nationally representative sample of the urban/rural population according to the Greek census 2011, a proportionate stratified by region systematic sampling procedure was used to recruit particpants. Data collection was guided through a structured questionnaire. Regarding willingness to COVID-19 vaccination, participants were asked to answer the following question: “If there was a vaccine available for the novel coronavirus, would you do it?” Results Of 1004 respondents only 57.7% stated that they are going to get vaccinated for COVID-19. Respondents aged > 65 years old, those who either themselves or a member of their household belonged to a vulnerable group, those believing that the COVID-19 virus was not developed in laboratories by humans, those believing that coronavirus is far more contagious and lethal compared to the H1N1 virus, and those believing that next waves are coming were statistically significantly more likely to be willing to get a COVID-19 vaccine. Higher knowledge score regarding symptoms, transmission routes and prevention and control measures against COVID-19 was significantly associated with higher willingness of respondents to get vaccinated. Conclusion A significant proportion of individuals in the general population are unwilling to receive a COVID-19 vaccine, stressing the need for public health officials to take immediate awareness-raising measures.

scholarly journals Economic Impacts of Banning Subtherapeutic Use of Antibiotics in Swine Production

2002 ◽  
Vol 34 (3) ◽  
pp. 489-500 ◽  
Author(s):  
B. Wade Brorsen ◽  
Terry Lehenbauer ◽  
Dasheng Ji ◽  
Joe Connor
Keyword(s):  
Public Health ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Economic Effects ◽  
Swine Production ◽  
Livestock Feed ◽  
Long Run ◽  
Short Run ◽  
Public Health Officials ◽  
Growth Promotants

Public health officials and physicians are concerned about possible development of bacterial resistance and potential effects on human health that may be related to the use of antimicrobial agents in livestock feed. The focus of this research is aimed at determining the economic effects that subtherapeutic bans of antimicrobials would have on both swine producers and consumers. The results show that a ban on growth promotants for swine would be costly, totaling $242.5 million annually, with swine producers sharing the larger portion in the short run and consumers sharing the larger portion in the long run.

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