scholarly journals TAF8 regions important for TFIID lobe B assembly, or for TAF2 interactions, are required for embryonic stem cell survival

2021 ◽  
Author(s):  
Elisabeth Scheer ◽  
Jie Luo ◽  
Frank Ruffenach ◽  
Jean-Marie Garnier ◽  
Isabelle Kolb-Cheynel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Survival ◽  
Rna Polymerase Ii ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Gene Promoters ◽  
Pol Ii ◽  
Histone Fold ◽  
Stem Cell Survival

The human general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). In eukaryotic cells, TFIID is thought to nucleate RNA polymerase II (Pol II) preinitiation complex formation on all protein coding gene promoters and thus, be crucial for Pol II transcription. TFIID is composed of three lobes, named A, B and C. Structural studies showed that TAF8 forms a histone fold pair with TAF10 in lobe B and participates in connecting lobe B to lobe C. In the present study, we have investigated the requirement of the different regions of TAF8 for in vitro TFIID assembly, and the importance of certain TAF8 regions for mouse embryonic stem cell (ESC) viability. We have identified a TAF8 region, different from the histone fold domain of TAF8, important for assembling with the 5TAF core complex in lobe B, and four regions of TAF8 each individually required for interacting with TAF2 in lobe C. Moreover, we show that the 5TAF core-interacting TAF8 domain, and the proline rich domain of TAF8 that interacts with TAF2, are both required for mouse embryonic stem cell survival. Thus, our study demonstrates that distinct TAF8 regions involved in connecting lobe B to lobe C are crucial for TFIID function and consequent ESC survival.

scholarly journals THAP1: Role in Mouse Embryonic Stem Cell Survival and Differentiation

2017 ◽  
Vol 9 (1) ◽  
pp. 92-107 ◽  
Author(s):  
Francesca Aguilo ◽  
Zuchra Zakirova ◽  
Katie Nolan ◽  
Ryan Wagner ◽  
Rajal Sharma ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Survival ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Survival

scholarly journals Large-scale dissection suggests that ultraconserved elements are dispensable for mouse embryonic stem cell survival and fitness

2019 ◽  
Author(s):  
Aksana Schneider ◽  
Michael Hiller ◽  
Frank Buchholz
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Survival ◽  
Large Scale ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Ultraconserved Elements ◽  
Stem Cell Survival ◽  
Unexpected Findings

Withdrawal statementThe authors have withdrawn their manuscript from bioRxiv regretfully. During additional experiments for the revision we noticed inconsistencies in some results, which may affect the main conclusion of the manuscript. In particular, we noticed that using different primer pairs, some clones that were classified as homozygous knockouts, are actually not knockouts. These were unexpected findings, but might reflect the recently described larger rearrangements observed using CRISPR technology1,2,3,4. Follow up experiments are ongoing, but we would like to withdraw the manuscript at this point in time to avoid misunderstandings. Therefore, the manuscript should currently not be cited. Any questions regarding the manuscript should be addressed to the corresponding author.

Neurotrophins mediate human embryonic stem cell survival

2006 ◽  
Vol 24 (3) ◽  
pp. 344-350 ◽  
Author(s):  
April D Pyle ◽  
Leslie F Lock ◽  
Peter J Donovan
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Survival ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Survival

Trauma-Associated Inflammatory Response Impairs Embryonic Stem Cell Survival and Integration after Implantation into Injured Rat Brain

2007 ◽  
Vol 24 (4) ◽  
pp. 625-637 ◽  
Author(s):  
Marek Molcanyi ◽  
Peter Riess ◽  
Kristine Bentz ◽  
Marc Maegele ◽  
Jürgen Hescheler ◽  
...  
Keyword(s):  
Stem Cell ◽  
Inflammatory Response ◽  
Embryonic Stem Cell ◽  
Cell Survival ◽  
Rat Brain ◽  
Embryonic Stem ◽  
Stem Cell Survival

scholarly journals SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells

2021 ◽  
Author(s):  
Veronique Fischer ◽  
Elisabeth Scheer ◽  
Elisabeth Lata ◽  
Bastien Morlet ◽  
Damien Plassard ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Mammalian Cells ◽  
Embryonic Stem ◽  
Gene Promoters ◽  
Saga Complex ◽  
Self Renewal ◽  
Pol Ii ◽  
Histone Fold ◽  
Specific Subset

Coactivator complexes regulate chromatin accessibility and transcription. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is an evolutionary conserved coactivator complex. The core module scaffolds the entire SAGA complex and adopts a histone octamer-like structure, which consists of six histone fold domain (HFD)-containing proteins forming three histone fold (HF) pairs, to which the double HFD-containing SUPT3H adds an HF pair. Spt3, the yeast ortholog of SUPT3H, interacts genetically and biochemically with the TATA binding protein (TBP) and contributes to global RNA polymerase II (Pol II) transcription. Here we demonstrate that i) SAGA purified from human U2OS or mouse embryonic stem cells (mESC) can assemble without SUPT3H; ii) SUPT3H is not essential for mESC survival, iii) SUPT3H is required for mESC growth and self-renewal, and iv) the loss of SUPT3H from mammalian cells affects the transcription of only a specific subset of genes. Accordingly, in the absence of SUPT3H no major change in TBP accumulation at gene promoters was observed. Thus, SUPT3H is not required for the assembly of SAGA, TBP recruitment, or overall Pol II transcription, but plays a role in mESC growth and self-renewal. Our data further suggest that yeast and mammalian SAGA complexes contribute to transcription regulation by distinct mechanisms.

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