scholarly journals An integrated pipeline and multi-model graphical user interface for accurate nano-dosimetry

2021 ◽  
Author(s):  
Ermes Botte ◽  
Pietro Vagaggini ◽  
Ilaria Zanoni ◽  
Davide Gardini ◽  
Anna Luisa Costa ◽  
...  
Keyword(s):  
Effective Dose ◽  
Graphical User Interface ◽  
Engineered Nanoparticles ◽  
Dose Estimation ◽  
Cell Type ◽  
Exposure System ◽  
Cell Monolayers ◽  
Accurate Dose ◽  
Mathematical Operations

Accurate dosing of nanoparticles is crucial for risk assessment and for their safe use in medical and other applications. Although it is well-known that nanoparticles sediment, diffuse and aggregate as they move through a fluid, and that therefore the effective dose perceived by cells may not necessarily be that initially administered, dose quantification remains a challenge. This is because to date, methods for accurate dose estimation are difficult to implement, involving precise characterization of the nanomaterial and the exposure system as well as complex mathematical operations. Here we present a pipeline for accurate nano-dosimetry of engineered nanoparticles on cell monolayers, based on an easy-to-use graphical software - DosiGUI - which integrates two well-established particokinetic and particodynamic models. DosiGUI is an open source tool which was developed to facilitate nano-dosimetrics. The pipeline includes methods for determining the stickiness index which describes the affinity between nanoparticles and cells. Our results show that accurate estimations of the effective dose cannot prescind from rigorous characterization of the stickiness index, which depends on both nanoparticle characteristics and cell type.

Current trends and challenges in analysis and characterization of engineered nanoparticles in seawater

Talanta ◽  
2021 ◽  
Vol 226 ◽  
pp. 122201
Author(s):  
Andrei R. Timerbaev ◽  
Olga V. Kuznetsova ◽  
Bernhard K. Keppler
Keyword(s):  
Engineered Nanoparticles ◽  
Current Trends

scholarly journals Connectivity characterization of the mouse basolateral amygdalar complex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Houri Hintiryan ◽  
Ian Bowman ◽  
David L. Johnson ◽  
Laura Korobkova ◽  
Muye Zhu ◽  
...  
Keyword(s):  
Granular Cell ◽  
Cell Types ◽  
Projection Neurons ◽  
Cell Type ◽  
Connectivity Map ◽  
Analysis Techniques ◽  
Domain Specific ◽  
Cell Type Specific ◽  
Unique Domain

AbstractThe basolateral amygdalar complex (BLA) is implicated in behaviors ranging from fear acquisition to addiction. Optogenetic methods have enabled the association of circuit-specific functions to uniquely connected BLA cell types. Thus, a systematic and detailed connectivity profile of BLA projection neurons to inform granular, cell type-specific interrogations is warranted. Here, we apply machine-learning based computational and informatics analysis techniques to the results of circuit-tracing experiments to create a foundational, comprehensive BLA connectivity map. The analyses identify three distinct domains within the anterior BLA (BLAa) that house target-specific projection neurons with distinguishable morphological features. We identify brain-wide targets of projection neurons in the three BLAa domains, as well as in the posterior BLA, ventral BLA, posterior basomedial, and lateral amygdalar nuclei. Inputs to each nucleus also are identified via retrograde tracing. The data suggests that connectionally unique, domain-specific BLAa neurons are associated with distinct behavior networks.

Cytometric Profiling of CD133+ Cells in Human Colon Carcinoma Cell Lines Identifies a Common core Phenotype and Cell Type-specific Mosaics

2013 ◽  
Vol 28 (3) ◽  
pp. 267-273 ◽  
Author(s):  
Marica Gemei ◽  
Rosa Di Noto ◽  
Peppino Mirabelli ◽  
Luigi Del Vecchio
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Cell Type ◽  
Carcinoma Cell Lines ◽  
Cell Type Specific

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

Characterization of cell type-specific S100B expression in the mouse olfactory bulb

Cell Calcium ◽  
2021 ◽  
Vol 94 ◽  
pp. 102334
Author(s):  
Xin Su ◽  
Tamara Vasilkovska ◽  
Nicole Fröhlich ◽  
Olga Garaschuk
Keyword(s):  
Olfactory Bulb ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals Addendum to Bell et al's “In-depth characterization of the salivary adenoid cystic carcinoma transcriptome with emphasis on dominant cell type”

Cancer ◽  
2016 ◽  
Vol 122 (14) ◽  
pp. 2278-2279 ◽  
Author(s):  
Diana Bell ◽  
Achim H. Bell ◽  
Jolanta Bondaruk ◽  
Ehab Y. Hanna ◽  
Randall S. Weber
Keyword(s):  
Adenoid Cystic Carcinoma ◽  
Cell Type ◽  
Salivary Adenoid Cystic Carcinoma ◽  
Adenoid Cystic

scholarly journals Comprehensive and cell-type-based characterization of the dorsal midbrain during development

2018 ◽  
Vol 24 (1) ◽  
pp. 41-59 ◽  
Author(s):  
Nariko Arimura ◽  
Ken-ichi Dewa ◽  
Mako Okada ◽  
Yuchio Yanagawa ◽  
Shin-ichiro Taya ◽  
...  
Keyword(s):  
Cell Type

scholarly journals Functional modeling of tight junctions in intestinal cell monolayers using polyethylene glycol oligomers

2001 ◽  
Vol 281 (2) ◽  
pp. C388-C397 ◽  
Author(s):  
C. J. Watson ◽  
M. Rowland ◽  
G. Warhurst
Keyword(s):  
Cell Lines ◽  
Pore Radius ◽  
Intestinal Cell ◽  
Sodium Caprate ◽  
Cell Monolayers ◽  
Functional Regulation ◽  
Glycol Oligomers ◽  
Molecular Radius ◽  
Sieving Model

Despite significant advances in the characterization of tight junction (TJ) proteins, little is known about how molecular changes relate to function due primarily to the limitations of conventional paracellular probes. To address this, the paracellular pathway in Caco-2 and T84 cell lines was profiled by measuring the permeabilities of 24 polyethylene glycols (PEG) of increasing molecular radius (3.5–7.4 Å) analyzed by mass spectrometry. When combined with a paracellular sieving model, these data provided quantitative descriptors of the pathway under control conditions and after exposure to TJ modulators. PEG profiles in both cell lines conformed to a biphasic process involving a restrictive pore (radius 4.3–4.5 Å) and a nonrestrictive component responsible for permeability of larger molecules. PEG profiling revealed significant differences between the effects of EGTA and sodium caprate (C10). The restrictive component of EGTA-treated cells lost all size discrimination due to an increase in pore radius. Sodium caprate had no effect on pore radius but increased permeability via a different mechanism possibly involving increased numbers of functional pores. PEG profiling provides a useful tool for probing the functional regulation of the paracellular route.

Sign in / Sign up

Export Citation Format

Share Document