Genome-wide identification of protein binding sites in mammalian cells
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AbstractWe present GWPBS-Cap, a method to capture genome-wide protein binding sites (PBSs) without using antibodies. Using this technique, we identified many protein binding sites with different binding strengths between proteins and DNA. The PBSs can be useful to predict transcription binding sites and the co-localization of multiple transcription factors in the genome. The results also revealed that active promoters contained more protein binding sites with lower NaCl tolerances. Taken together, GWPBS-Cap can be used to efficiently identify protein binding sites and reveal genome-wide landscape of DNA-protein interactions.
2019 ◽
Vol 508
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pp. 953-958
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2012 ◽
Vol 109
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pp. 2370-2375
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1991 ◽
Vol 11
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pp. 1894-1900
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1991 ◽
Vol 11
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pp. 1894-1900
1971 ◽
Vol 68
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pp. S223-S246
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2004 ◽
Vol 11
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pp. 281-290
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