scholarly journals MLF2 modulates phase separated nuclear envelope condensates that provoke dual proteotoxicity

2021 ◽  
Author(s):  
Sarah M Prophet ◽  
Anthony J Rampello ◽  
Robert F Niescier ◽  
Juliana E Shaw ◽  
Anthony J Koleske ◽  
...  
Keyword(s):  
Phase Separation ◽  
Nuclear Envelope ◽  
Nuclear Pore ◽  
Disease Manifestation ◽  
Disease Etiology ◽  
Aaa Atpase ◽  
Dyt1 Dystonia ◽  
Chaperone Network ◽  
Neurological Movement Disorder

DYT1 dystonia is a highly debilitating neurological movement disorder arising from mutation in the AAA+ ATPase TorsinA. The hallmark of Torsin dysfunction is nuclear envelope blebbing resulting from defects in nuclear pore complex biogenesis. Whether blebs actively contribute to disease manifestation is presently unknown. We report that FG-nucleoporins in the bleb lumen undergo phase separation and contribute to DYT1 dystonia by provoking two proteotoxic insults. Short-lived ubiquitinated proteins that are normally rapidly degraded in healthy cells partition into the bleb lumen and become stabilized. Additionally, blebs selectively sequester a chaperone network composed of HSP70s and HSP40s. The composition of this chaperone network is altered by the bleb component MLF2. We further demonstrate that MLF2 is a catalyst of phase separation that suppresses the ectopic accumulation of FG-nucleoporins and modulates the selective properties and size of condensates in vitro. Our studies identify unprecedented, dual mechanisms of proteotoxicity in the context of liquid-liquid phase separation with direct implications for our understanding of disease etiology and treatment.

scholarly journals Expression of TorsinA in a heterologous yeast system reveals interactions with conserved lumenal domains of LINC and nuclear pore complexes

2018 ◽  
Author(s):  
Madeleine Chalfant ◽  
Karl W. Barber ◽  
Sapan Borah ◽  
David Thaller ◽  
C. Patrick Lusk
Keyword(s):  
Nuclear Envelope ◽  
Genetic Interaction ◽  
Spindle Pole ◽  
Nuclear Pore ◽  
Physical Interaction ◽  
Nuclear Pore Complexes ◽  
Gene Encoding ◽  
Aaa Atpase ◽  
Dyt1 Dystonia ◽  
Pore Complexes

ABSTRACTDYT1 dystonia is caused by an in-frame deletion of a glutamic acid codon in the gene encoding the AAA+ ATPase TorsinA. TorsinA localizes within the lumen of the nuclear envelope/ER and binds to a membrane-spanning co-factor, LAP1 or LULL1, to form an ATPase; the substrate(s) of TorsinA remain ill defined. Here we use budding yeast, which lack Torsins, to interrogate TorsinA function. We show that TorsinA accumulates at nuclear envelope embedded spindle pole bodies (SPBs) in a way that requires its oligomerization and the conserved SUN-domain protein, Mps3. TorsinA is released from SPBs upon expression of LAP1 and stabilized by LAP1 mutants incapable of stimulating TorsinA ATPase activity, suggesting the recapitulation of a TorsinA-substrate cycle. While the expression of TorsinA or TorsinA-ΔE impacts the fitness of strains expressing mps3 alleles, a genetic interaction with a conserved component of the nuclear pore complex, Pom152, is specific for TorsinA. This specificity is mirrored by a physical interaction between Pom152 and TorsinA, but not TorsinA-ΔE. These data suggest that TorsinA-nucleoporin interactions would be abrogated by TorsinA-ΔE, providing new experimental avenues to interrogate the molecular basis behind nuclear envelope herniations seen in cells lacking TorsinA function.

scholarly journals Expression of TorsinA in a heterologous yeast system reveals interactions with lumenal domains of LINC and nuclear pore complex components

2019 ◽  
Vol 30 (5) ◽  
pp. 530-541 ◽  
Author(s):  
Madeleine Chalfant ◽  
Karl W. Barber ◽  
Sapan Borah ◽  
David Thaller ◽  
C. Patrick Lusk
Keyword(s):  
Nuclear Envelope ◽  
Nuclear Pore Complex ◽  
Mammalian Cells ◽  
Spindle Pole ◽  
Nuclear Pore ◽  
Gene Encoding ◽  
Aaa Atpase ◽  
Dyt1 Dystonia ◽  
Physiological Relevance ◽  
Membrane Spanning

DYT1 dystonia is caused by an in-frame deletion of a glutamic acid codon in the gene encoding the AAA+ ATPase TorsinA (TorA). TorA localizes within the lumen of the nuclear envelope/endoplasmic reticulum and binds to a membrane-spanning cofactor, lamina associated polypeptide 1 (LAP1) or lumenal domain like LAP1 (LULL1), to form an ATPase; the substrate(s) of TorA remains ill-defined. Here we use budding yeast, which lack Torsins, to interrogate TorA function. We show that TorA accumulates at nuclear envelope-embedded spindle pole bodies (SPBs) in a way that requires its oligomerization and the SUN (Sad1 and UNc-84)-domain protein, Mps3. We further show that TorA physically interacts with human SUN1/2 within this system, supporting the physiological relevance of these interactions. Consistent with the idea that TorA acts on a SPB substrate, its binding to SPBs is modulated by the ATPase-stimulating activity of LAP1. TorA and TorA-ΔE reduce the fitness of cells expressing mps3 alleles, whereas TorA alone inhibits growth of cells lacking Pom152, a component of the nuclear pore complex. This genetic specificity is mirrored biochemically as TorA, but not TorA-ΔE, binds Pom152. Thus, TorA–nucleoporin interactions might be abrogated by TorA-ΔE, suggesting new experimental avenues to interrogate the molecular basis behind nuclear envelope herniations seen in mammalian cells lacking TorA function.

scholarly journals The luminal AAA+ ATPase torsinA mediates distinct mechanisms of nuclear-cytoplasmic communication by adopting different functional assembly states.

2021 ◽  
Author(s):  
Kwang-Ho Hur ◽  
Jared W. Hennen ◽  
Cosmo A Saunders ◽  
Amy Schoenhoefen ◽  
Patrick T Willey ◽  
...  
Keyword(s):  
Nuclear Pore ◽  
Linc Complex ◽  
Complex Assembly ◽  
Aaa Atpase ◽  
Cellular Assays ◽  
Dyt1 Dystonia ◽  
Helical Polymer ◽  
Aaa Protein

Chemical and mechanical nuclear-cytoplasmic communication across the nuclear envelope (NE) is largely mediated by the nuclear pore complex (NPC) and the linker of nucleoskeleton and cytoskeleton (LINC) complex, respectively. While NPC and LINC complex assembly are functionally related, the mechanisms responsible for this relationship remain poorly understood. Here, we investigated how the luminal ATPases associated with various cellular activities (AAA+) protein torsinA promotes NPC and LINC complex assembly using fluorescence fluctuation spectroscopy (FFS), quantitative photobleaching analyses, and functional cellular assays. We report that torsinA controls LINC complex-dependent nuclear-cytoskeletal coupling as a soluble hexameric AAA+ protein and interphase NPC biogenesis as a membrane-associated helical polymer. These findings help resolve the conflicting models of torsinA function that were recently proposed based on in vitro structural studies. Our results will enable future studies of the role of defective nuclear-cytoplasmic communication in DYT1 dystonia and other diseases caused by mutations in torsinA.

scholarly journals The Interaction of Epac1 and Ran Promotes Rap1 Activation at the Nuclear Envelope

2010 ◽  
Vol 30 (16) ◽  
pp. 3956-3969 ◽  
Author(s):  
Chang Liu ◽  
Maho Takahashi ◽  
Yanping Li ◽  
Tara J. Dillon ◽  
Stefanie Kaech ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
G Protein ◽  
Nuclear Pore ◽  
Intracellular Camp ◽  
Small G Protein ◽  
Proliferation And Differentiation ◽  
Ras Family ◽  
Rap1 Activation

ABSTRACT Epac1 (exchange protein directly activated by cyclic AMP [cAMP]) couples intracellular cAMP to the activation of Rap1, a Ras family GTPase that regulates cell adhesion, proliferation, and differentiation. Using mass spectrometry, we identified the small G protein Ran and Ran binding protein 2 (RanBP2) as potential binding partners of Epac1. Ran is a small G protein best known for its role in nuclear transport and can be found at the nuclear pore through its interaction with RanBP2. Here we demonstrate that Ran-GTP and Epac1 interact with each other in vivo and in vitro. This binding requires a previously uncharacterized Ras association (RA) domain in Epac1. Surprisingly, the interaction of Epac1 with Ran is necessary for the efficient activation of Rap1 by Epac1. We propose that Ran and RanBP2 anchor Epac1 to the nuclear pore, permitting cAMP signals to activate Rap1 at the nuclear envelope.

scholarly journals LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells

2017 ◽  
Vol 114 (11) ◽  
pp. E2166-E2175 ◽  
Author(s):  
Mingyu Gu ◽  
Dollie LaJoie ◽  
Opal S. Chen ◽  
Alexander von Appen ◽  
Mark S. Ladinsky ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Fission Yeast ◽  
Nuclear Membrane ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Human Cells ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Loss Of Function

Endosomal sorting complexes required for transport III (ESCRT-III) proteins have been implicated in sealing the nuclear envelope in mammals, spindle pole body dynamics in fission yeast, and surveillance of defective nuclear pore complexes in budding yeast. Here, we report that Lem2p (LEM2), a member of the LEM (Lap2-Emerin-Man1) family of inner nuclear membrane proteins, and the ESCRT-II/ESCRT-III hybrid protein Cmp7p (CHMP7), work together to recruit additional ESCRT-III proteins to holes in the nuclear membrane. InSchizosaccharomyces pombe, deletion of the ATPasevps4leads to severe defects in nuclear morphology and integrity. These phenotypes are suppressed by loss-of-function mutations that arise spontaneously inlem2orcmp7, implying that these proteins may function upstream in the same pathway. Building on these genetic interactions, we explored the role of LEM2 during nuclear envelope reformation in human cells. We found that CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery during this window of cell division and that CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro. We further found that, during nuclear envelope formation, recruitment of the ESCRT factors CHMP7, CHMP2A, and IST1/CHMP8 all depend on LEM2 in human cells. We conclude that Lem2p/LEM2 is a conserved nuclear site-specific adaptor that recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope.

scholarly journals Decreased mechanotransduction prevents nuclear collapse in aCaenorhabditis eleganslaminopathy

2020 ◽  
Vol 117 (49) ◽  
pp. 31301-31308
Author(s):  
Gabriela Huelgas-Morales ◽  
Mark Sanders ◽  
Gemechu Mekonnen ◽  
Tatsuya Tsukamoto ◽  
David Greenstein
Keyword(s):  
Nuclear Envelope ◽  
Complex Function ◽  
Nuclear Lamina ◽  
Premature Aging ◽  
Nuclear Pore ◽  
Linc Complex ◽  
Force Transmission ◽  
Cell Nuclei ◽  
Aaa Atpase ◽  
C Elegans

The function of the nucleus depends on the integrity of the nuclear lamina, an intermediate filament network associated with the linker of nucleoskeleton and cytoskeleton (LINC) complex. The LINC complex spans the nuclear envelope and mediates nuclear mechanotransduction, the process by which mechanical signals and forces are transmitted across the nuclear envelope. In turn, the AAA+ ATPase torsinA is thought to regulate force transmission from the cytoskeleton to the nucleus. In humans, mutations affecting nuclear envelope-associated proteins cause laminopathies, including progeria, myopathy, and dystonia, though the extent to which endogenous mechanical stresses contribute to these pathologies is unclear. Here, we use theCaenorhabditis elegansgermline as a model to investigate mechanisms that maintain nuclear integrity as germ cell nuclei progress through meiotic development and migrate for gametogenesis—processes that require LINC complex function. We report that decreasing the function of theC. eleganstorsinA homolog, OOC-5, rescues the sterility and premature aging caused by a null mutation in the single worm lamin homolog. We show that decreasing OOC-5/torsinA activity prevents nuclear collapse in lamin mutants by disrupting the function of the LINC complex. At a mechanistic level, OOC-5/torsinA promotes the assembly or maintenance of the lamin-associated LINC complex and this activity is also important for interphase nuclear pore complex insertion into growing germline nuclei. These results demonstrate that LINC complex-transmitted forces damage nuclei with a compromised nuclear lamina. Thus, the torsinA–LINC complex nexus might comprise a therapeutic target for certain laminopathies by preventing damage from endogenous cellular forces.

scholarly journals LULL1 Retargets TorsinA to the Nuclear Envelope Revealing an Activity That Is Impaired by the DYT1 Dystonia Mutation

2009 ◽  
Vol 20 (11) ◽  
pp. 2661-2672 ◽  
Author(s):  
Abigail B. Vander Heyden ◽  
Teresa V. Naismith ◽  
Erik L. Snapp ◽  
Didier Hodzic ◽  
Phyllis I. Hanson
Keyword(s):  
Nuclear Envelope ◽  
Membrane Structure ◽  
Wild Type ◽  
Nuclear Pores ◽  
Native Page ◽  
Aaa Atpase ◽  
Dyt1 Dystonia ◽  
Blue Native Page ◽  
Transient Interaction ◽  
Blue Native

TorsinA (TorA) is an AAA+ ATPase in the endoplasmic reticulum (ER) lumen that is mutated in early onset DYT1 dystonia. TorA is an essential protein in mice and is thought to function in the nuclear envelope (NE) despite localizing throughout the ER. Here, we report that transient interaction of TorA with the ER membrane protein LULL1 targets TorA to the NE. FRAP and Blue Native PAGE indicate that TorA is a stable, slowly diffusing oligomer in either the absence or presence of LULL1. Increasing LULL1 expression redistributes both wild-type and disease-mutant TorA to the NE, while decreasing LULL1 with shRNAs eliminates intrinsic enrichment of disease-mutant TorA in the NE. When concentrated in the NE, TorA displaces the nuclear membrane proteins Sun2, nesprin-2G, and nesprin-3 while leaving nuclear pores and Sun1 unchanged. Wild-type TorA also induces changes in NE membrane structure. Because SUN proteins interact with nesprins to connect nucleus and cytoskeleton, these effects suggest a new role for TorA in modulating complexes that traverse the NE. Importantly, once concentrated in the NE, disease-mutant TorA displaces Sun2 with reduced efficiency and does not change NE membrane structure. Together, our data suggest that LULL1 regulates the distribution and activity of TorA within the ER and NE lumen and reveal functional defects in the mutant protein responsible for DYT1 dystonia.

scholarly journals In cell architecture of the nuclear pore complex and snapshots of its turnover

2020 ◽  
Author(s):  
Matteo Allegretti ◽  
Christian E. Zimmerli ◽  
Vasileios Rantos ◽  
Florian Wilfling ◽  
Paolo Ronchi ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Mrna Export ◽  
Light And Electron Microscopy ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Formidable Challenge ◽  
Cellular Context ◽  
Pore Complexes

SummaryNuclear pore complexes (NPCs) mediate exchange across the nuclear envelope. They consist of hundreds of proteins called nucleoporins (Nups) that assemble in multiple copies to fuse the inner and outer nuclear membranes. Elucidating the molecular function and architecture of NPCs imposes a formidable challenge and requires the convergence of in vitro and in situ approaches. How exactly NPC architecture accommodates processes such as mRNA export or NPC assembly and turnover inside of cells remains poorly understood. Here we combine integrated in situ structural biology, correlative light and electron microscopy with yeast genetics to structurally analyze NPCs within the native context of Saccharomyces cerevisiae cells under conditions of starvation and exponential growth. We find an unanticipated in situ layout of nucleoporins with respect to overall dimensions and conformation of the NPC scaffold that could not have been predicted from previous in vitro analysis. Particularly striking is the configuration of the Nup159 complex, which appears critical to spatially accommodate not only mRNA export but also NPC turnover by selective autophagy. We capture structural snapshots of NPC turnover, revealing that it occurs through nuclear envelope herniae and NPC-containing nuclear vesicles. Our study provides the basis for understanding the various membrane remodeling events that happen at the interface of the nuclear envelope with the autophagy apparatus and emphasizes the need of investigating macromolecular complexes in their cellular context.

scholarly journals The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis

2015 ◽  
Vol 208 (6) ◽  
pp. 671-681 ◽  
Author(s):  
J. Sebastian Gomez-Cavazos ◽  
Martin W. Hetzer
Keyword(s):  
Cell Differentiation ◽  
Nuclear Envelope ◽  
Er Stress ◽  
Myoblast Differentiation ◽  
Nuclear Pore ◽  
C2c12 Myoblast ◽  
Terminal Domain ◽  
Er Homeostasis

Previously, we identified the nucleoporin gp210/Nup210 as a critical regulator of muscle and neuronal differentiation, but how this nucleoporin exerts its function and whether it modulates nuclear pore complex (NPC) activity remain unknown. Here, we show that gp210/Nup210 mediates muscle cell differentiation in vitro via its conserved N-terminal domain that extends into the perinuclear space. Removal of the C-terminal domain, which partially mislocalizes gp210/Nup210 away from NPCs, efficiently rescues the differentiation defect caused by the knockdown of endogenous gp210/Nup210. Unexpectedly, a gp210/Nup210 mutant lacking the NPC-targeting transmembrane and C-terminal domains is sufficient for C2C12 myoblast differentiation. We demonstrate that the endoplasmic reticulum (ER) stress-specific caspase cascade is exacerbated during Nup210 depletion and that blocking ER stress-mediated apoptosis rescues differentiation of Nup210-deficient cells. Our results suggest that the role of gp210/Nup210 in cell differentiation is mediated by its large luminal domain, which can act independently of NPC association and appears to play a pivotal role in the maintenance of nuclear envelope/ER homeostasis.

scholarly journals Nuclear Envelope Breakdown Is Coordinated by Both Nup358/RanBP2 and Nup153, Two Nucleoporins with Zinc Finger Modules

2006 ◽  
Vol 17 (2) ◽  
pp. 760-769 ◽  
Author(s):  
Amy J. Prunuske ◽  
Jin Liu ◽  
Suzanne Elgort ◽  
Jomon Joseph ◽  
Mary Dasso ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Zinc Finger ◽  
Nuclear Lamina ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Zinc Finger Domain ◽  
Membrane Remodeling ◽  
Nuclear Envelope Breakdown ◽  
Pore Complexes

When higher eukaryotic cells transition into mitosis, the nuclear envelope, nuclear pore complexes, and nuclear lamina are coordinately disassembled. The COPI coatomer complex, which plays a major role in membrane remodeling at the Golgi, has been implicated in the process of nuclear envelope breakdown and requires interactions at the nuclear pore complex for recruitment to this new site of action at mitosis. Nup153, a resident of the nuclear pore basket, was found to be involved in COPI recruitment, but the molecular nature of the interface between COPI and the nuclear pore has not been fully elucidated. To better understand what occurs at the nuclear pore at this juncture, we have probed the role of the nucleoporin Nup358/RanBP2. Nup358 contains a repetitive zinc finger domain with overall organization similar to a region within Nup153 that is critical to COPI association, yet inspection of these two zinc finger domains reveals features that also clearly distinguish them. Here, we found that the Nup358 zinc finger domain, but not a zinc finger domain from an unrelated protein, binds to COPI and dominantly inhibits progression of nuclear envelope breakdown in an assay that robustly recapitulates this process in vitro. Moreover, the Nup358 zinc finger domain interferes with COPI recruitment to the nuclear rim. Consistent with a role for this pore protein in coordinating nuclear envelope breakdown, Nup358-specific antibodies impair nuclear disassembly. Significantly, targeting either Nup153 or Nup358 for inhibition perturbs nuclear envelope breakdown, supporting a model in which these nucleoporins play nonredundant roles, perhaps contributing to COPI recruitment platforms on both the nuclear and cytoplasmic faces of the pore. We found that an individual zinc finger is the minimal interface for COPI association, although tandem zinc fingers are optimal. These results provide new information about the critical components of nuclear membrane remodeling and lay the foundation for a better understanding of how this process is regulated.

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