scholarly journals Systemic and intra-amygdala administrations of midazolam reverse anxiety-like behavior induced by cohabiting with a cagemate in chronic pain condition

2021 ◽  
Author(s):  
Isabela M Carmona ◽  
Paulo E Carneiro de Oliveira ◽  
Daniela Baptista-de-Souza ◽  
Azair Canto-de-Souza
Keyword(s):  
Chronic Pain ◽  
Elevated Plus Maze ◽  
Benzodiazepine Receptors ◽  
Emotional Contagion ◽  
Chronic Pain Condition ◽  
Affective Component ◽  
Anxiogenic Effect ◽  
Neural Processes ◽  
Plus Maze ◽  
Pathological Anxiety

The affective component of pain may be shared among conspecifics through emotional contagion, a form of empathic expression. In this sense, reverberation of negative emotions could generate distress behavioral responses, such as pathological anxiety. Evidences reported that amygdala and its benzodiazepine receptors are involved in perception of pain in others. However, relatively little is known about the neural processes underlying emotional contagion induced by pain observation. In the present study, we investigated the effects of midazolam, an allosteric GABAergic receptor agonist, in anxiety-like behaviors induced by cohabitation with cagemate submitted to sciatic nerve constriction. For this purpose, we administrated systemic (0.5, 1.0 and 2.0 mg/kg) and intra-amygdala midazolam injections (3.0 and 30.0 nmol) in observer cagemates before elevated plus-maze (EPM) evaluation. We found that mice subjected to nerve constriction and their observer cagemates increased anxiety-like behavior in the EPM. Further, systemically (1.0 and 2.0 mg/kg) and intra-amygdala administration of midazolam (3.0 and 30 nmol) reverse this anxiogenic effect. Collectively, these results suggest that social interaction with a cagemate under chronic pain produces anxiety-like responses that could be blocked through midazolam application.

scholarly journals N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 892 ◽  
Author(s):  
Rade Vukovic ◽  
Igor Kumburovic ◽  
Jovana Joksimovic Jovic ◽  
Nemanja Jovicic ◽  
Jelena S. Katanic Stankovic ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Control Group ◽  
Albino Rats ◽  
Behavioral Testing ◽  
Anxiogenic Effect ◽  
Plus Maze ◽  
Wistar Albino Rats ◽  
Cisplatin Therapy

Since cisplatin therapy is usually accompanied with numerous toxicities, including neurotoxicity, that involve tissue oxidative damage, the aim of this study was to evaluate the possible protective effect of N-acetylcysteine (NAC) on the anxiogenic response to cisplatin (CIS). Thirty-two male Wistar albino rats divided into four groups (control, cisplatin, NAC, and CIS + NAC). All treatments were delivered intraperitoneally. On day one, the control and cisplatin groups received saline while the NAC and CIS + NAC groups were administered with NAC (500 mg/kg). On the fifth day, the control group received saline while the CIS group was treated with cisplatin (7.5 mg/kg), the NAC group again received NAC (500 mg/kg), and the CIS + NAC group was simultaneously treated with cisplatin and NAC (7.5 and 500 mg/kg, respectively). Behavioral testing, performed on the tenth day in the open field (OF) and elevated plus maze (EPM) tests, revealed the anxiogenic effect of cisplatin that was significantly attenuated by NAC. The hippocampal sections evaluation showed increased oxidative stress (increased lipid peroxidation and decline in antioxidant enzymes activity) and proapoptotic action (predominantly by diminished antiapoptotic gene expression) following a single dose of cisplatin. NAC supplementation along with cisplatin administration reversed the prooxidative and proapoptotic effects of cisplatin. In conclusion, the results obtained in this study confirmed that antioxidant supplementation with NAC may attenuate the cisplatin-induced anxiety. The mechanism of anxiolytic effect achieved by NAC may include the decline in oxidative damage that down regulates increased apoptosis and reverses the anxiogenic action of cisplatin.

Anticompulsive effects of novel derivatives of coumarin in rats

2021 ◽  
Vol 19 (3) ◽  
pp. 339-344
Author(s):  
Bakhodir B. Daliev ◽  
Eugenii R. Bychkov ◽  
Leonid V. Myznikov ◽  
Andrei A. Lebedev ◽  
Petr D. Shabanov
Keyword(s):  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Emotional Behavior ◽  
Central Action ◽  
Elevated Plus Maze Test ◽  
Anxiogenic Effect ◽  
Plus Maze ◽  
Few Data ◽  
New Compounds ◽  
Dose Dependent

BACKGROUND: Until now, the neurotropic effect, in particular the effect on the emotional behavior of oxy-coumarins, has not been adequately studied. There are only few data on their central action. Currently, research is underway on the synthesis of new compounds based on natural oxy-coumarins, which will potentially have a higher biological activity. AIM: Was to study the central action of new oxycoumarin-based compounds IEM-2886, LVM-99, LVM-S144, in particular, on compulsive behavior in rats. MATERIALS AND METHODS: To assess the behavior of Wistar rats, the Marble-test and Elevated plus maze methods were used. Oxycoumarin derivatives (IEM-2886, LVM-99, LVM-S144) were injected intraperitoneally at doses of 1, 10 and 25 mg/kg. The effectiveness of the drugs was judged by the number of balls buried in the Marble test and by the duration of staying in the open and closed sleeves of the Elevated plus maze. Results. It was shown that in the Marble test, oxycoumarin-based compounds (IEM-2886, LVM-99, LVM-S144) caused a decrease in the number of buried balls, which shows their anti-compulsive effect. After administration of IEM-2886, LVM-99, LVM-S144 (125 mg / kg) compounds, dose-dependent effects were observed (p 0.05). The elevated plus maze test did not show the anxiolytic effect typical for tranquilizers. Moreover, after the administration of IEM-2886 and LVM-S144 at a dose of 25 mg / kg, an increase in the time spent in the closed sleeve of the maze (p 0.05) was observed, i.e. an anxiogenic effect. CONCLUSION: Thus, oxy-coumarin-based compounds are selective for the assessment of anticompulsive effects.

Anxiogenic effect of sleep deprivation in the elevated plus-maze test in mice

2004 ◽  
Vol 176 (2) ◽  
pp. 115-122 ◽  
Author(s):  
Regina H. Silva ◽  
Sonia R. Kameda ◽  
Rita C. Carvalho ◽  
Andr� L. Takatsu-Coleman ◽  
Suzy T. Niigaki ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Elevated Plus Maze ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Anxiogenic Effect ◽  
Plus Maze

scholarly journals The Evaluation of the Effects of N-Acetylcysteine on Cisplatin-Induced Alterations in Exploratory Activity in Elevated Plus Maze Test in Rats

2019 ◽  
Vol 20 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Milica Pantic ◽  
Milos Minic
Keyword(s):  
Beneficial Effect ◽  
Elevated Plus Maze ◽  
Exploratory Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Elevated Plus Maze Test ◽  
Anxiogenic Effect ◽  
Plus Maze ◽  
Significant Attenuation ◽  
Cisplatin Therapy

Abstract The aim of this study was to evaluate the potential beneficial effect of N-acetylcysteine (NAC) on cisplatin-induced alterations in anxiety levels in rats, by means of parameters of the exploratory activity obtained in the elevated plus maze (EPM) test. Animals were divided into four groups: control group, cisplatin group (7.5 mg/kg/weekly of cisplatin), N-acetylcysteine group (500 mg/kg/weekly of NAC), and cisplatin plus N-acetylcysteine group (7.5 mg/kg/weekly of cisplatin, and 500 mg/kg/weekly of NAC). After two weeks of treatment, exploratory activity (estimated by means of the number of rearings, head-dippings and the number of total exploratory activity episodes) was significantly reduced in cisplatin group comparing to control values. Although NAC induced no alterations in exploratory activity when applied alone, simultaneous administration with cisplatin resulted in significant attenuation of cisplatin-induced decline in exploratory activity. The exploratory activity gradually decreased in time-dependent manner during five minutes of EPM test in all groups. The results of this study confirmed clear beneficial effect of NAC supplementation against cisplatin- induced neurotoxicity in rats. Antioxidative properties of NAC were manifested through restoration of exploratory activity, confirming that NAC administration can attenuate anxiogenic effect of cisplatin therapy. Those results could recommend NAC supplementation as a potential protection against cisplatin-induced neurotoxicity.

scholarly journals SHORT-TERM SOCIAL ISOLATION DOES NOT REDUCE ELEVATED PLUS-MAZE EXPLORATION IN EARLY PROTEIN MALNOURISHED RATS

2016 ◽  
Vol 10 (2) ◽  
Author(s):  
Sebastião De Sousa Almeida ◽  
Marielena De Araújo ◽  
Gabriela M. S. Moreira ◽  
Rodrigo V. F. Paiva ◽  
Luiz Marcellino De Oliveira
Keyword(s):  
Social Isolation ◽  
Elevated Plus Maze ◽  
Chow Diet ◽  
Short Term ◽  
Early Protein ◽  
Central Platform ◽  
Anxiogenic Effect ◽  
Plus Maze ◽  
Socially Isolated ◽  
Behavior Profile

An increased number of visits and time spent in the open arms of the elevated plus-maze by malnourished rats has been used as indicative of lower anxiety or impulsiveness. In order to study how this behavior profile responds to an anxiogenic procedure (short-term social isolation), control (16% protein) and malnourished (6% protein) rats were socially isolated prior to the test in the maze. Litters (dam plus 6 male 2 female pups) were fed the diets from birth to 49 days of age. From 50 days on, all rats were fed a lab chow diet. Social isolation consists in removing the rats from the group and placing in individual cages for 2h before the test. During the test each rat was individually placed on the center of the maze and allowed to explore for 5 min. The results showed higher open arms exploration and lower attempts to enter open arms by the malnourished rats than by the controls. Social isolation decreased open arm exploration and increased time spent on the central platform in control animals, but had no effect on the malnourished rats. The results reinforce the lower anxiety or higher impulsiveness of malnourished rats, as well as the anxiogenic effect of social isolation in control rats. However, the malnourished rats were unresponsive to the anxiogenic effects of social isolation, indicating that protein deficiency early in life not only induces lower anxiety or higher impulsiveness in the maze, but also changes the behavior of these animals in response to another environmentally-induced procedure of anxiety (social isolation). Keywords: Early protein malnutrition, Social isolation, Anxiety, Impulsiveness, Stress, Rats. 

scholarly journals Anxiolytic and Antidepressant-Like Effects of the Aqueous Extract ofAlafia multifloraStem Barks in Rodents

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Harquin Simplice Foyet ◽  
David Emery Tsala ◽  
Armand Abdou Bouba ◽  
Lucian Hritcu
Keyword(s):  
Aqueous Extract ◽  
Elevated Plus Maze ◽  
Benzodiazepine Receptors ◽  
Anxiety And Depression ◽  
Antidepressant Effect ◽  
Latency Time ◽  
Plus Maze ◽  
Antidepressant Effects ◽  
Immobility Time ◽  
Rats And Mice

The present study examined the anxiolytic and antidepressant effects of the aqueous extract ofAlafia multiflora Stapf(AM) stem barks (150 and 300 mg/kg, 7 days administration) on rats and mice, using experimental paradigms of anxiety and depression. In the open field, the aqueous extract increased significantly the number of center square crossed and the time spent at the center of the field as well as the rearing time, while the grooming time was reduced significantly. In the elevated plus maze, the aqueous extract increased the time spent and the number of entries in the open arms. All these effects were also completely reversed by flumazenil, an antagonist of benzodiazepine receptors and pindolol aβ-adrenoceptors blocker/5-HT 1A/1B receptor antagonist. The time spent in the light compartment, the latency time, and the number of the light-dark transitions increased significantly in the light/dark exploration test after the treatment with AM. The extract was able to reduce significantly the immobility time and increase swimming as well as climbing duration. Taken together, the present work evidenced anxiolytic effects of the aqueous extract of AM that might involve an action on benzodiazepine-type receptors and an antidepressant effect where noradrenergic mechanisms will probably play a role.

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