scholarly journals Endothelin receptor Aa regulates proliferation and differentiation of Erb-dependant pigment progenitors in zebrafish

2018 ◽  
Author(s):  
Karen Camargo-Sosa ◽  
Sarah Colanesi ◽  
Jeanette Müller ◽  
Stefan Schulte-Merker ◽  
Derek Stemple ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Blood Vessels ◽  
Communication Process ◽  
Pigment Cells ◽  
Quiescent State ◽  
Dependent Manner ◽  
Endothelin Receptor ◽  
Pigment Pattern ◽  
Pigment Patterns

AbstractSkin pigment patterns are important, being under strong selection for multiple roles including camouflage and UV protection. Pigment cells underlying these patterns form from adult pigment stem cells (APSCs). In zebrafish, APSCs derive from embryonic neural crest cells, but sit dormant until activated to produce pigment cells during metamorphosis. The APSCs are set-aside in an ErbB signaling dependent manner, but the mechanism maintaining quiescence until metamorphosis remains unknown. Mutants for a pigment pattern gene, parade, exhibit ectopic pigment cells localised to the ventral trunk. We show that parade encodes Endothelin receptor Aa, expressed in the blood vessels. Using chemical genetics, coupled with analysis of cell fate studies, we show that the ectopic pigment cells derive from APSCs. We propose that a novel population of APSCs exists in association with medial blood vessels, and that their quiescence is dependent upon Endothelin-dependent factors expressed by the blood vessels.Lay AbstractPigment patterns are crucial for the many aspects of animal biology, for example, providing camouflage, enabling mate selection and protecting against UV irradiation. These patterns are generated by one or more pigment cell-types, localised in the skin, but derived from specialised stem cells (adult pigment stem cells, APSCs). In mammals, such as humans, but also in birds and fish, these APSCs derive from a transient population of multipotent progenitor cells, the neural crest. Formation of the adult pigment pattern is perhaps best studied in the zebrafish, where the adult pigment pattern is formed during a metamorphosis beginning around 21 days of development. The APSCs are set-aside in the embryo around 1 day of development, but then remain inactive until that metamorphosis, when they become activated to produce the adult pigment cells. We know something of how the cells are set-aside, but what signals maintain them in an inactive state is a mystery. Here we study a zebrafish mutant, called parade, which shows ectopic pigment cells in the embryo. We clone the parade gene, identifying it as ednraa encoding a component of a cell-cell communication process, which is expressed in blood vessels. By characterising the changes in the neural crest and in the pigment cells formed, and by combining this with an innovative assay identifying drugs that prevent the ectopic cells from forming, we deduce that the ectopic cells in the larva derive from precocious activation of APSCs to form pigment cells. We propose that a novel population of APSCs are associated with the blood vessels, that these are held in a quiescent state by signals coming from these vessels, and that these signals depend upon ednraa. Together this opens up an exciting opportunity to identify the signals maintaining APSC quiescence in zebrafish.

An orthologue of the kit-related gene fms is required for development of neural crest-derived xanthophores and a subpopulation of adult melanocytes in the zebrafish, Danio rerio

Development ◽  
2000 ◽  
Vol 127 (14) ◽  
pp. 3031-3044 ◽  
Author(s):  
D.M. Parichy ◽  
D.G. Ransom ◽  
B. Paw ◽  
L.I. Zon ◽  
S.L. Johnson
Keyword(s):  
Neural Crest ◽  
Danio Rerio ◽  
Receptor Tyrosine Kinase ◽  
Pigment Cell ◽  
Pigment Cells ◽  
Pigment Pattern ◽  
Cell Behaviors ◽  
Pattern Development ◽  
And Migration ◽  
Pigment Patterns

Developmental mechanisms underlying traits expressed in larval and adult vertebrates remain largely unknown. Pigment patterns of fishes provide an opportunity to identify genes and cell behaviors required for postembryonic morphogenesis and differentiation. In the zebrafish, Danio rerio, pigment patterns reflect the spatial arrangements of three classes of neural crest-derived pigment cells: black melanocytes, yellow xanthophores and silver iridophores. We show that the D. rerio pigment pattern mutant panther ablates xanthophores in embryos and adults and has defects in the development of the adult pattern of melanocyte stripes. We find that panther corresponds to an orthologue of the c-fms gene, which encodes a type III receptor tyrosine kinase and is the closest known homologue of the previously identified pigment pattern gene, kit. In mouse, fms is essential for the development of macrophage and osteoclast lineages and has not been implicated in neural crest or pigment cell development. In contrast, our analyses demonstrate that fms is expressed and required by D. rerio xanthophore precursors and that fms promotes the normal patterning of melanocyte death and migration during adult stripe formation. Finally, we show that fms is required for the appearance of a late developing, kit-independent subpopulation of adult melanocytes. These findings reveal an unexpected role for fms in pigment pattern development and demonstrate that parallel neural crest-derived pigment cell populations depend on the activities of two essentially paralogous genes, kit and fms.

Zebrafish Pigment Pattern Formation: Insights into the Development and Evolution of Adult Form

2019 ◽  
Vol 53 (1) ◽  
pp. 505-530 ◽  
Author(s):  
Larissa B. Patterson ◽  
David M. Parichy
Keyword(s):  
Pattern Formation ◽  
Pigment Cell ◽  
Cell Lineage ◽  
Pigment Cells ◽  
Cellular Interactions ◽  
Lineage Specification ◽  
Pigment Pattern ◽  
Adult Form ◽  
Pattern Development ◽  
Pigment Patterns

Vertebrate pigment patterns are diverse and fascinating adult traits that allow animals to recognize conspecifics, attract mates, and avoid predators. Pigment patterns in fish are among the most amenable traits for studying the cellular basis of adult form, as the cells that produce diverse patterns are readily visible in the skin during development. The genetic basis of pigment pattern development has been most studied in the zebrafish, Danio rerio. Zebrafish adults have alternating dark and light horizontal stripes, resulting from the precise arrangement of three main classes of pigment cells: black melanophores, yellow xanthophores, and iridescent iridophores. The coordination of adult pigment cell lineage specification and differentiation with specific cellular interactions and morphogenetic behaviors is necessary for stripe development. Besides providing a nice example of pattern formation responsible for an adult trait of zebrafish, stripe-forming mechanisms also provide a conceptual framework for posing testable hypotheses about pattern diversification more broadly. Here, we summarize what is known about lineages and molecular interactions required for pattern formation in zebrafish, we review some of what is known about pattern diversification in Danio, and we speculate on how patterns in more distant teleosts may have evolved to produce a stunningly diverse array of patterns in nature.

scholarly journals A pluri- és multipotencia határán: a ganglionléc őssejtjei

2015 ◽  
Vol 156 (42) ◽  
pp. 1683-1694
Author(s):  
Gyöngyi Kudlik ◽  
Zsolt Matula ◽  
Tamás Kovács ◽  
S. Veronika Urbán ◽  
Ferenc Uher
Keyword(s):  
Stem Cells ◽  
Nervous System ◽  
Neural Crest ◽  
Cell Biology ◽  
Pigment Cells ◽  
Stem Cell Biology ◽  
Vertebrate Embryos ◽  
Derivatives Of ◽  
Review Current ◽  
Migratory Cell

The neural crest is a transient, multipotent, migratory cell population that is unique to vertebrate embryos and gives rise to many derivatives, ranging from the neuronal and glial components of the peripheral nervous system to the ectomesenchymal derivatives of the craniofacial area and pigment cells in the skin. Intriguingly, the neural crest derived stem cells are not only present in the embryonic neural crest, but also in their target tissues in the fetus and adult. These postmigratory stem cells, at least partially, resemble their multipotency. Moreover, fully differentiated neural crest-derived cells such as Schwann cells and melanocytes are able to dedifferentiate into stem-like progenitors. Here the authors review current understanding of this unique plasticity and its potential application in stem cell biology as well as in regenerative medicine. Orv. Hetil., 2015, 156(42), 1683–1694.

scholarly journals Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-derived Stem Cells

2021 ◽  
Author(s):  
Beatrice A. Windmöller ◽  
Anna L. Höving ◽  
Cornelius Knabbe ◽  
Johannes F. W. Greiner
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Genetic Heterogeneity ◽  
Single Cells ◽  
Inferior Turbinate ◽  
Dependent Manner ◽  
Developmental Potential ◽  
Adult Human ◽  
Marker Proteins ◽  
Heterogeneous Expression

Abstract Adult human neural crest-derived stem cells (NCSCs) are found in a variety of adult tissues and show an extraordinary broad developmental potential. Despite their great differentiation capacity, increasing evidence suggest a remaining niche-dependent variability between different NCSC-populations regarding their differentiation behavior and expression signatures. In the present study, we extended the view on heterogeneity of NCSCs by identifying heterogeneous expression levels and protein amounts of characteristic markers even between NCSCs from the same niche of origin. In particular, populations of neural crest-derived inferior turbinate stem cells (ITSCs) isolated from different individuals showed significant variations in characteristic NCSC marker proteins Nestin, S100 and Slug in a donor-dependent manner. Notably, increased nuclear protein amounts of Slug were accompanied by a significantly elevated level of nuclear NF-κB-p65 protein, suggesting an NF-κB-dependent regulation of NCSC-makers. In addition to this interpopulational genetic heterogeneity of ITSC-populations from different donors, single ITSCs also revealed a strong heterogeneity regarding the protein amounts of Nestin, S100, Slug and NF-κB-p65 even within the same clonal culture. Our present findings therefor strongly suggest ITSC-heterogeneity to be at least partly based on an interpopulational genetic heterogeneity dependent on the donor accompanied by a stochastic intrapopulational heterogeneity between single cells. We propose this stochastic intrapopulational heterogeneity to occur in addition to the already described genetic variability between clonal NCSC-cultures and the niche-dependent plasticity of NCSCs. Our observations offer a novel perspective on NCSC-heterogeneity, which may build the basis to understand heterogeneous NCSC-behavior. Graphical Abstract

Mechanisms of pigment pattern formation in the quail embryo

Development ◽  
1990 ◽  
Vol 109 (1) ◽  
pp. 81-89 ◽  
Author(s):  
M.K. Richardson ◽  
A. Hornbruch ◽  
L. Wolpert
Keyword(s):  
Pattern Formation ◽  
Neural Crest ◽  
Larval Fish ◽  
Dorsal Surface ◽  
Neural Crest Cells ◽  
Mirror Image ◽  
Differential Migration ◽  
Pigment Pattern ◽  
Pigment Patterns ◽  
Local Cues

One hypothesis to account for pigment patterning in birds is that neural crest cells migrate into all feather papillae. Local cues then act upon the differentiation of crest cells into melanocytes. This hypothesis is derived from a study of the quail-chick chimaera (Richardson et al., Development 107, 805–818, 1989). Another idea, derived from work on larval fish and amphibia, is that pigment patterns arise from the differential migration of crest cells. We want to know which of these mechanisms can best account for pigment pattern formation in the embryonic plumage of the quail wing. Most of the feather papillae on the dorsal surface of the wing are pigmented, while many on the ventral surface are white. When ectoderm from unpigmented feather papillae is grown in culture, it gives rise to melanocytes. This indicates that neural crest cells are present in white feathers but that they fail to differentiate. If the wing tip is inverted experimentally then the pigment pattern is inverted also. This is difficult to explain in terms of a model based on migratory pathways, unless one assumes that the pathways became re-routed. When an extra polarizing region is grafted to the anterior margin of the wing bud, a duplication develops in: (1) the pattern of skeletal elements; (2) the pattern of feather papillae; (3) the feather pigment pattern. The pigment pattern was not a precise mirror image although some groups of papillae showed a high degree of symmetry in their pigmentation. Both the tip inversions and the duplications produce discontinuities in the feather and pigment patterns. No evidence of intercalation was found in these cases. We conclude that pigment patterning in birds is determined by local cues acting on melanocyte differentiation, rather than by the differential migration of crest cells. Positional values along the anteroposterior axis of the pigment pattern are determined by a gradient of positional information. Thus the pigment patterns, feather patterns and cartilage patterns of the wing may all be specified by a similar mechanism.

scholarly journals Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-Derived Stem Cells

2021 ◽  
Author(s):  
Beatrice A. Windmöller ◽  
Anna L. Höving ◽  
Johannes F.W. Greiner
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Genetic Heterogeneity ◽  
Single Cells ◽  
Inferior Turbinate ◽  
Dependent Manner ◽  
Developmental Potential ◽  
Adult Human ◽  
Marker Proteins ◽  
Heterogeneous Expression

Abstract Adult human neural crest-derived stem cells (NCSCs) are found in a variety of adult tissues and show an extraordinary broad developmental potential. NCSCs are therefore promising candidates for applications in regenerative medicine, although increasing evidence suggest a remaining nichedependent variability between different NCSC-populations regarding their behavior and expression signatures. In the present study, we extended the view on heterogeneity of NCSCs by identifying heterogeneous expression levels and protein amounts of characteristic markers even between NCSCs from the same niche of origin. In particular, populations of neural crest-derived inferior turbinate stem cells (ITSCs) isolated from different individuals showed significant variations in characteristic NCSC marker proteins Nestin, S100 and Slug in a donor-dependent manner. Notably, increased nuclear protein amounts of Slug were accompanied by a significantly elevated level of nuclear NF-κB-p65 protein, suggesting an NF-κB-dependent regulation of NCSC-makers. In addition to this interpopulational genetic heterogeneity of ITSC-populations from different donors, single ITSCs also revealed a strong heterogeneity regarding the protein amounts of Nestin, S100, Slug and NF-κB-p65 even within the same clonal culture. Our present findings therefor strongly suggest ITSC-heterogeneity to be at least partly based on an interpopulational genetic heterogeneity dependent on the donor accompanied by a stochastic intrapopulational heterogeneity between single cells. We propose this stochastic intrapopulational heterogeneity to occur in addition to the already described genetic variability between clonal NCSC-cultures and the niche-dependent plasticity of NCSCs. Our observations offer a novel perspective on NCSC-heterogeneity, which may build the basis to understand heterogeneous NCSC-behavior and improve their clinical applicability.

scholarly journals Inter- and intrapopulational heterogeneity of characteristic markers in adult human neural crest-derived stem cells

2021 ◽  
Author(s):  
Beatrice A Windmoeller ◽  
Anna Hoeving ◽  
Johannes F.W. Greiner
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Genetic Heterogeneity ◽  
Single Cells ◽  
Inferior Turbinate ◽  
Dependent Manner ◽  
Developmental Potential ◽  
Adult Human ◽  
Marker Proteins ◽  
Heterogeneous Expression

Adult human neural crest-derived stem cells (NCSCs) are found in a variety of adult tissues and show an extraordinary broad developmental potential. NCSCs are therefore promising candidates for applications in regenerative medicine, although increasing evidence suggest a remaining niche-dependent variability between different NCSC-populations regarding their behavior and expression signatures. In the present study, we extended the view on heterogeneity of NCSCs by identifying heterogeneous expression levels and protein amounts of characteristic markers even between NCSCs from the same niche of origin. In particular, populations of neural crest-derived inferior turbinate stem cells (ITSCs) isolated from different individuals showed significant variations in characteristic NCSC marker proteins Nestin, S100 and Slug in a donor-dependent manner. Notably, increased nuclear protein amounts of Slug were accompanied by a significantly elevated level of nuclear NF-κB-p65 protein, suggesting an NF-κB-dependent regulation of NCSC-makers. In addition to this interpopulational genetic heterogeneity of ITSC-populations from different donors, single ITSCs also revealed a strong heterogeneity regarding the protein amounts of Nestin, S100, Slug and NF-κB-p65 even within the same clonal culture. Our present findings therefor strongly suggest ITSC-heterogeneity to be at least partly based on an interpopulational genetic heterogeneity dependent on the donor accompanied by a stochastic intrapopulational heterogeneity between single cells. We propose this stochastic intrapopulational heterogeneity to occur in addition to the already described genetic variability between clonal NCSC-cultures and the niche-dependent plasticity of NCSCs. Our observations offer a novel perspective on NCSC-heterogeneity, which may build the basis to understand heterogeneous NCSC-behavior and improve their clinical applicability.

scholarly journals Mutational Analysis of Endothelin Receptor b1 (rose) during Neural Crest and Pigment Pattern Development in the Zebrafish Danio rerio

2000 ◽  
Vol 227 (2) ◽  
pp. 294-306 ◽  
Author(s):  
David M. Parichy ◽  
Eve M. Mellgren ◽  
John F. Rawls ◽  
Susana S. Lopes ◽  
Robert N. Kelsh ◽  
...  
Keyword(s):  
Neural Crest ◽  
Danio Rerio ◽  
Mutational Analysis ◽  
Endothelin Receptor ◽  
Pigment Pattern ◽  
Pattern Development

Intercellular Relations in the Development of Amphibian Pigmentation

Development ◽  
1953 ◽  
Vol 1 (3) ◽  
pp. 263-268
Author(s):  
Victor C. Twitty
Keyword(s):  
Neural Crest ◽  
Pigment Cells ◽  
Pigment Pattern ◽  
Present Discussion ◽  
Amoeboid Cells

The role of intercellular influences in the control of differentiation finds many illustrations in the development of amphibian pigmentation. From the time the chromatophores leave the neural crest as colourless, amoeboid cells, until they come to rest as differentiated elements of the distinctive pigment pattern, their developmental behaviour is conditioned throughout by effects exerted mutually by the chromatophores themselves or imposed upon them by the cells of neighbouring tissues. For the purposes of the present discussion, I shall limit myself to examples of intercellular relations affecting (1) the migration of chromatoblasts and (2) their synthesis of pigment. Various suggestions have been made concerning the nature of the factors responsible for the dispersion of pro-pigment cells from the mid-dorsal line to their resting-places throughout the skin. One is that the cells are actually drawn ventrally in response to a true chemotactic attraction exerted by the skin beneath which they spread.

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