scholarly journals Protective effects of low-intensity pulsed ultrasound on mandibular condylar cartilage exposed to mechanical overloading

2018 ◽  
Author(s):  
Mutsumi Fujita ◽  
Minami Sato-Shigeta ◽  
Hiroki Mori ◽  
Akihiko Iwasa ◽  
Nobuhiko Kawai ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Mandibular Condyle ◽  
Protective Effects ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Early Progression ◽  
Experimental Group

AbstractThe aim of this study was to examine the role of low-intensity pulsed ultrasound (LIPUS) exposure in the onset and early progression of temporomandibular joint (TMJ) osteoarthritis (TMJ-OA) induced by mechanical overloading. Fifteen-week-old male Wistar rats were divided into two experimental groups and a control group (n = 5 each). In the experimental groups, both TMJs were subjected to mechanical overloading by forced mouth opening using a jaw-opening device for 3 h/day for 5 continuous days. After mechanical overloading, TMJs in one experimental group were exposed to LIPUS for 20 min/day. After the experiments, mandibles were resected from the rats, and the condyles were processed. The bones were analyzed using high-resolution microcomputed tomography (micro-CT). The resected TMJs were also subjected to histological analysis and immunohistochemical staining. Micro-CT images of the mandibular condyle showed severe subchondral trabecular bone loss in the experimental group with overloading. Treatment with LIPUS after overloading resulted in decreased subchondral trabecular bone resorption. In TMJ sections from the experimental group with overloading, cell-free regions and proteoglycan loss characterized the cartilage degradation; LIPUS exposure restricted these changes in the mandibular condyle. Furthermore, the number of tartrate-resistant acid phosphatase-positive osteoclasts in the mineralized layer of the condylar cartilage increased after mechanical overloading and decreased after LIPUS treatment. Our findings suggest that LIPUS exposure after mechanical TMJ overloading downregulates subchondral trabecular bone resorption and proteoglycan loss in the mandibular condylar cartilage. Thus, it may prove to be protective effects of LIPUS exposure on onset and early progression of TMJ-OA induced by mechanical overloading.

scholarly journals Low-Intensity Pulsed Ultrasound Stimulation of Condylar Growth in Rats

2009 ◽  
Vol 79 (5) ◽  
pp. 964-970 ◽  
Author(s):  
Rodrigo Oyonarte ◽  
Mariana Zárate ◽  
Francisco Rodriguez
Keyword(s):  
Layer Thickness ◽  
Mandibular Condyle ◽  
Sprague Dawley ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Mandibular Growth ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Bone Level ◽  
And Control

Abstract Objective: To test the hypothesis that low-intensity pulsed ultrasound (LIPUS) stimulation does not histologically affect the growth of mandibular condylar cartilage. Materials and Methods: Thirty-five 20-day-old Sprague-Dawley rats were assigned to experimental and control groups. Experimental rats were stimulated with LIPUS in the temporomandibular joint (TMJ) region unilaterally, for 10 or 20 minutes for 20 days. After euthanasia, histological specimens were analyzed qualitatively and histomorphometrically at the anterior and posterior aspects of the mandibular condyle, including the condylar cartilage and the area and perimeter of subchondral bony trabeculae. Results: LIPUS stimulation may alter the histological arrangement of the condylar bone and cartilage, showing qualitative differences on specimens treated for 10 or 20 minutes daily compared with controls. Cartilaginous layer thickness was not affected by LIPUS stimulation to a significant level, but was modified at the relative layer thickness within the cartilage at the anterior aspect of the condyle (P < .05). At the subchondral bone level, 20-minute stimulation significantly increases trabecular perimeter (P = .01). Conclusions: LIPUS application may affect mandibular growth pattern in rats acting at the cartilage and bone level. The effect of LIPUS on the growing condyle is expressed through a variation in trabecular shape and perimeter. A greater response is achieved when stimulated for 20 minutes instead of 10 minutes daily.

scholarly journals Effect of low-intensity pulsed ultrasound (LIPUS) on mandibular condyle growth in rats analyzed with micro-CT

2016 ◽  
Vol 58 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Kyozo Sasaki ◽  
Mitsuru Motoyoshi ◽  
Eri Horinuki ◽  
Yoshinori Arai ◽  
Noriyoshi Shimizu
Keyword(s):  
Mandibular Condyle ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Chondro-protective effects of low intensity pulsed ultrasound

2016 ◽  
Vol 24 (11) ◽  
pp. 1989-1998 ◽  
Author(s):  
S.M.Z. Uddin ◽  
B. Richbourgh ◽  
Y. Ding ◽  
A. Hettinghouse ◽  
D.E. Komatsu ◽  
...  
Keyword(s):  
Protective Effects ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Low-intensity pulsed ultrasound stimulation for mandibular condyle osteoarthritis lesions in rats

Oral Diseases ◽  
2017 ◽  
Vol 24 (4) ◽  
pp. 600-610 ◽  
Author(s):  
A Kanaguchi Arita ◽  
I Yonemitsu ◽  
Y Ikeda ◽  
M Miyazaki ◽  
T Ono
Keyword(s):  
Mandibular Condyle ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

The Effect of Low Intensity Pulsed Ultrasound on Proliferation of Rat Condylar Cartilage Cells in Hypoxia Culture

2019 ◽  
Vol 9 (5) ◽  
pp. 630-636
Author(s):  
Qiong Li ◽  
Wei Geng ◽  
Jun Li ◽  
Chao Liang ◽  
Chaolumen Bao
Keyword(s):  
Treatment Effect ◽  
Temporomandibular Disorder ◽  
Mrna Level ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Improvement Effect ◽  
Cartilage Cells ◽  
Subchondral Bone Tissue

Temporomandibular disorder (TMD) is a common and frequently-occurring oral and maxillofacial disease. Restoration of the joint's cartilage and subchondral bone tissue is the key to treat TMD. Low intensity pulsed ultrasound (LIPUS) has been proved to be effective in facilitating chondrocyte's proliferation, which inspired us to probe its treatment effect on TMD. With an isolated and hypoxic cultured mandibular condylar chondrocytes (MCC), we established a model for TMD. HIF-1α and VEGF's mRNA level was increased in hypoxic cultured MCC, which indicates our model simulated the cell's status caused by TMD pathology. When stimulated with LIIPUS at 45 mW/cm2, the MCC's apoptosis rate was inhibited and its proliferation was improved with best efficiency. Further experiment revealed that LIPUS restored MCC's Metalloproteinase's (MMP) up-regulation and collagen II's downregulation in hypoxic cultured MCC, respectively, which might be related to LIPUS's improvement effect on MCC.

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