scholarly journals Correction: Effects of Low-Intensity Pulsed Ultrasound on New Trabecular Bone during Bone-Tendon Junction Healing in a Rabbit Model: A Synchrotron Radiation Micro-CT Study

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0214974
Author(s):  
Hongbin Lu ◽  
Cheng Zheng ◽  
Zhanwen Wang ◽  
Can Chen ◽  
Huabin Chen ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Trabecular Bone ◽  
Rabbit Model ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Protective effects of low-intensity pulsed ultrasound on mandibular condylar cartilage exposed to mechanical overloading

2018 ◽  
Author(s):  
Mutsumi Fujita ◽  
Minami Sato-Shigeta ◽  
Hiroki Mori ◽  
Akihiko Iwasa ◽  
Nobuhiko Kawai ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Mandibular Condyle ◽  
Protective Effects ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Early Progression ◽  
Experimental Group

AbstractThe aim of this study was to examine the role of low-intensity pulsed ultrasound (LIPUS) exposure in the onset and early progression of temporomandibular joint (TMJ) osteoarthritis (TMJ-OA) induced by mechanical overloading. Fifteen-week-old male Wistar rats were divided into two experimental groups and a control group (n = 5 each). In the experimental groups, both TMJs were subjected to mechanical overloading by forced mouth opening using a jaw-opening device for 3 h/day for 5 continuous days. After mechanical overloading, TMJs in one experimental group were exposed to LIPUS for 20 min/day. After the experiments, mandibles were resected from the rats, and the condyles were processed. The bones were analyzed using high-resolution microcomputed tomography (micro-CT). The resected TMJs were also subjected to histological analysis and immunohistochemical staining. Micro-CT images of the mandibular condyle showed severe subchondral trabecular bone loss in the experimental group with overloading. Treatment with LIPUS after overloading resulted in decreased subchondral trabecular bone resorption. In TMJ sections from the experimental group with overloading, cell-free regions and proteoglycan loss characterized the cartilage degradation; LIPUS exposure restricted these changes in the mandibular condyle. Furthermore, the number of tartrate-resistant acid phosphatase-positive osteoclasts in the mineralized layer of the condylar cartilage increased after mechanical overloading and decreased after LIPUS treatment. Our findings suggest that LIPUS exposure after mechanical TMJ overloading downregulates subchondral trabecular bone resorption and proteoglycan loss in the mandibular condylar cartilage. Thus, it may prove to be protective effects of LIPUS exposure on onset and early progression of TMJ-OA induced by mechanical overloading.

Low-intensity Pulsed Ultrasound Does Not Enhance Distraction Callus in a Rabbit Model

2007 ◽  
Vol 459 ◽  
pp. 237-245 ◽  
Author(s):  
Kenneth F Taylor ◽  
Bahman Rafiee ◽  
John E Tis ◽  
Nozumu Inoue
Keyword(s):  
Rabbit Model ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Low-intensity pulsed ultrasound reduces periodontal atrophy in occlusal hypofunctional teeth

2017 ◽  
Vol 87 (5) ◽  
pp. 709-716 ◽  
Author(s):  
Yuki Kasahara ◽  
Risa Usumi-Fujita ◽  
Jun Hosomichi ◽  
Sawa Kaneko ◽  
Yuji Ishida ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Bhlh Transcription Factor ◽  
Mrna Levels ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Qrt Pcr ◽  
Low Intensity ◽  
Normal Occlusion ◽  
Ct Analysis

ABSTRACT Objective: To clarify whether low-intensity pulsed ultrasound (LIPUS) exposure has recovery effects on the hypofunctional periodontal ligament (PDL) and interradicular alveolar bone (IRAB). Materials and Methods: Twelve-week-old male Sprague-Dawley rats were divided into three groups (n = 5 each): a normal occlusion (C) group, an occlusal hypofunction (H) group, and an occlusal hypofunction group subjected to LIPUS (HL) treatment. Hypofunctional occlusion of the maxillary first molar (M1) of the H and HL groups was induced by the bite-raising technique. Only the HL group was irradiated with LIPUS for 5 days. The IRAB and PDL of M1 were examined by microcomputed tomography (micro-CT) analysis. To quantify mRNA expression of cytokines involved in PDL proliferation and development, real-time reverse transcription quantitative PCR (qRT-PCR) was performed for twist family bHLH transcription factor 1 (Twist1), periostin, and connective tissue growth factor (CTGF) in the PDL samples. Results: Micro-CT analysis showed that the PDL volume was decreased in the H group compared with that of the C and HL groups. Both bone volume per tissue volume (BV/TV) of IRAB was decreased in the H group compared with that in the C group. LIPUS exposure restored BV/TV in the IRAB of the HL group. qRT-PCR analysis showed that Twist1, periostin, and CTGF mRNA levels were decreased in the H group and increased in the HL group. Conclusion: LIPUS exposure reduced the atrophic changes of alveolar bone by inducing the upregulation of periostin and CTGF expression to promote PDL healing after induction of occlusal hypofunction.

Effect of Low-Intensity Pulsed Ultrasound After Autologous Adipose-Derived Stromal Cell Transplantation for Bone-Tendon Healing in a Rabbit Model

2019 ◽  
Vol 47 (4) ◽  
pp. 942-953 ◽  
Author(s):  
Can Chen ◽  
Tao Zhang ◽  
Fei Liu ◽  
Jin Qu ◽  
Yang Chen ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Volume Fraction ◽  
Treatment Time ◽  
Rabbit Model ◽  
The Other ◽  
Pulsed Ultrasound ◽  
Trabecular Thickness ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Background: Low-intensity pulsed ultrasound (LIPUS), as a safe biophysiotherapy, can enhance bone-tendon (B-T) healing in vivo and induce osteogenic or chondrogenic differentiation of mesenchymal stromal cells in vitro. This study aimed to determine whether LIPUS can improve the efficacy of transplanted mesenchymal stromal cells on B-T healing. Hypothesis: LIPUS can induce lineage-specific differentiation of transplanted adipose-derived stromal cells (ASCs) at the B-T healing site, thus resulting in superior healing quality when compared with LIPUS or ASCs alone. Study Design: Controlled laboratory study. Methods: A total of 112 mature rabbits with partial patellectomy in the hindlimb were randomly assigned into mock sonication without ASCs (control), ultrasonication without ASCs (LIPUS), mock sonication with ASCs (ASCs), and ultrasonication with ASCs (LIPUS + ASCs). The treatment time of the mock sonication or ultrasonication was 20 minutes per day. Autologous ASCs were transplanted to the healing site by fibrin glue during the operation, and LIPUS was delivered daily starting at postoperative day 3 until euthanasia. The patella–patellar tendon junctions were postoperatively harvested at 8 and 16 weeks for radiological, histological, and mechanical evaluations. Additionally, 9 animals were used for ASC tracking with mCherry protein. Results: Radiologically, there was more new bone formation and remodeling in the LIPUS + ASCs group as compared with the other groups. Synchrotron radiation micro–computed tomography showed that the LIPUS + ASCs group significantly increased bone volume fraction, trabecular thickness, and trabecular number at the healing site as compared with the other groups at postoperative 8 weeks ( P < .05 for all). Histologically, immunohistochemical staining confirmed that the transplanted mCherry-ASCs can differentiate into osteoblasts and fibrochondrocytic-like cells. Meanwhile, as compared with the other groups, the LIPUS + ASCs group showed more formation and maturity of the fibrocartilage layer and new bone at postoperative weeks 8 and 16 ( P < .05 for all). Biomechanically, the LIPUS + ASCs group showed significantly higher failure load and stiffness versus the other groups at postoperative weeks 8 and 16 ( P < .05 for all). Conclusion: Autologous ASC transplantation stimulated with LIPUS can result in superior B-T healing quality when compared with LIPUS or ASCs alone. Clinical Relevance: This study demonstrates the effectiveness of using ASC transplantation stimulated with LIPUS for B-T healing and provides a foundation for future clinical studies.

Low-intensity pulsed ultrasound repair in mandibular condylar cartilage injury rabbit model

2019 ◽  
Vol 104 ◽  
pp. 60-66 ◽  
Author(s):  
Hang-yu Zhou ◽  
Qiang Li ◽  
Jian-xiong Wang ◽  
Yu-jie Xie ◽  
Shi-qi Wang ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Cartilage Injury ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Mandibular Condylar Cartilage
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