scholarly journals Tracking disease resistance deployment in potato breeding by enrichment sequencing

2018 ◽  
Author(s):  
Miles R Armstrong ◽  
Jack Vossen ◽  
Tze Yin Lim ◽  
Ronald C B Hutten ◽  
Jianfei Xu ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Genes ◽  
Potato Breeding ◽  
Crop Improvement ◽  
Resistance Breeding ◽  
Cost Effective ◽  
Complete Sequence ◽  
Gene Enrichment ◽  
Disease Resistance Breeding ◽  
Late Blight Pathogen

SummaryFollowing the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

scholarly journals 997 USE OF MOLECULAR MARKERS IN BREEDING FOR RESISTANCE TO FUNGAL PATHOGENS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 572c-572
Author(s):  
James D. Kell
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Resistance Genes ◽  
Fungal Pathogens ◽  
Rapd Markers ◽  
Resistance Breeding ◽  
Horticultural Crops ◽  
Allele Specific ◽  
Disease Resistance Breeding ◽  
Selection For

The use of RAPD markers in disease resistance breeding has been successfully demonstrated in horticultural crops. The identification of RAPD markers and their potential role in disease resistance breeding was first demonstrated in tomato; the procedure of bulk segregant analysis for detecting linkages between RAPD markers and genes conditioning resistance to downy mildew was described in lettuce; the use of linked RAPD markers to facilitate the efficient pyramiding of epistatic rust resistance genes to prolong their durability has been illustrated in common bean; the development of allele specific or sequence characterized primers linked to resistance genes has been achieved in pea, bean, tomato, and lettuce Indirect selection for disease resistance based on molecular markers is not a replacement for classical breeding procedures. Used as tools, markers offer breeders unique opportunities to combine resistance to an array of different pathogens and efficiently pyramid epistatic resistance genes for highly variable fungal pathogens. This would not always be possible in the absence of linked markers.

In vitro Selection: A Candidate Approach for Disease Resistance Breeding in Fruit Crops

2010 ◽  
Vol 9 (8) ◽  
pp. 437-446 ◽  
Author(s):  
Ramesh Chandra ◽  
Madhu Kamle ◽  
Anju Bajpai ◽  
M. Muthukumar ◽  
Shahina Kalim
Keyword(s):  
Disease Resistance ◽  
In Vitro Selection ◽  
Resistance Breeding ◽  
Fruit Crops ◽  
Disease Resistance Breeding

scholarly journals Advances in Wheat and Pathogen Genomics: Implications for Disease Control

2018 ◽  
Vol 56 (1) ◽  
pp. 67-87 ◽  
Author(s):  
Beat Keller ◽  
Thomas Wicker ◽  
Simon G. Krattinger
Keyword(s):  
Disease Resistance ◽  
Molecular Level ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
The Past ◽  
Wheat Improvement ◽  
Disease Resistance Breeding ◽  
Near Future ◽  
New Cultivars ◽  
Made In

The gene pool of wheat and its wild and domesticated relatives contains a plethora of resistance genes that can be exploited to make wheat more resilient to pathogens. Only a few of these genes have been isolated and studied at the molecular level. In recent years, we have seen a shift from classical breeding to genomics-assisted breeding, which makes use of the enormous advancements in DNA sequencing and high-throughput molecular marker technologies for wheat improvement. These genomic advancements have the potential to transform wheat breeding in the near future and to significantly increase the speed and precision at which new cultivars can be bred. This review highlights the genomic improvements that have been made in wheat and its pathogens over the past years and discusses their implications for disease-resistance breeding.

scholarly journals DNA markers for disease resistance breeding in peas (Pisum sativum L.)

1997 ◽  
Vol 50 ◽  
pp. 314-315 ◽  
Author(s):  
G. Timmerman-Vaughan ◽  
A.C. Russell ◽  
A. Hill ◽  
T.J. Frew ◽  
B.J. Gilpin
Keyword(s):  
Disease Resistance ◽  
Pisum Sativum ◽  
Dna Markers ◽  
Resistance Breeding ◽  
Disease Resistance Breeding
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