scholarly journals Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)

2018 ◽  
Author(s):  
Alden S. Estep ◽  
Neil D. Sanscrainte ◽  
Christy M. Waits ◽  
Sarah J. Bernard ◽  
Aaron M. Lloyd ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Genetic Markers ◽  
Aedes Albopictus ◽  
Topical Application ◽  
Pyrethroid Resistance ◽  
Management Program ◽  
Health Concern ◽  
Effective Control ◽  
Resistance Testing

AbstractRecent outbreaks of locally transmitted dengue and Zika viruses in Florida have placed more emphasis on the importance of integrated vector management plans for Aedes aegypti (L.) and Aedes albopictus Skuse. Adulticiding, primarily with pyrethroids, can be the best option available for the immediate control of potentially arbovirus-infected mosquitoes during outbreak situations. While pyrethroid resistance is common in Ae. aegypti worldwide and testing is recommended by CDC and WHO, resistance to this class of products has not been widely examined or quantified in Florida. To address this information gap, we performed the first study to quantify both pyrethroid resistance and genetic markers of pyrethroid resistance in Ae. aegypti and Ae. albopictus strains in Florida. Using direct topical application, we examined 21 Ae. aegypti strains from 9 counties and found permethrin resistance (resistance ratio (RR)=6-61-fold) in all strains when compared to the susceptible ORL1952 control strain. Permethrin resistance in five strains of Ae. albopictus was very low (RR<1.6) even when collected from the same containers producing resistant Ae. aegypti. Characterization of two sodium channel kdr alleles associated with pyrethroid-resistance showed widespread distribution in 62 strains of Ae. aegypti. The 1534 phenylalanine to cysteine (F1534C) single nucleotide polymorphism SNP was fixed or nearly fixed in all strains regardless of RR. We observed much more variation in the 1016 valine to isoleucine (V1016I) allele and observed that increasing frequency of the homozygous V1016I allele correlates strongly with increased RR (Pearson corr= 0.905). In agreement with previous studies, we observed a very low frequency of three kdr genotypes, IIFF, VIFF, and IIFC. In this study, we provide a statewide examination of pyrethroid resistance, and demonstrate that permethrin resistance and the genetic markers for resistance are widely present in FL Ae. aegypti. Resistance testing should be included in an effective management program.Author SummaryAedes aegypti and Aedes albopictus can vector a variety of arboviruses that cause diseases and are thus a public health concern. Pyrethroid insecticide resistance is common in Aedes aegypti in many locations worldwide and can adversely affect vector control operations. However, the resistance status of these vectors in Florida is largely unreported and recent local transmission of dengue and Zika viruses has made this information critical for effective control operations. In this study, we showed that permethrin resistance and two common SNPs of the voltage gated sodium channel (V1016I and F1534C) previously associated with pyrethroid resistance were widely present in Florida Aedes aegypti strains. We also observed a strong correlation between the IICC genotype and RR as determined by topical application, which suggests, as have others, that kdr frequency may be a useful indicator of resistance in Aedes aegypti.

scholarly journals Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

Insects ◽  
2016 ◽  
Vol 7 (4) ◽  
pp. 60 ◽  
Author(s):  
Yuzhe Du ◽  
Yoshiko Nomura ◽  
Boris Zhorov ◽  
Ke Dong
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Pyrethroid Resistance

scholarly journals KÊNH DẪN TRUYỀN NA+ CỔNG ĐIỆN ÁP (NaV) VÀ TÍNH KHÁNG THUỐC DIỆT CÔN TRÙNG Ở MUỖI TRUYỀN BỆNH SỐT XUẤT HUYẾT

2017 ◽  
Vol 15 (3) ◽  
pp. 393-401
Author(s):  
Nguyễn Thị Kim Liên ◽  
Nguyễn Thị Hương Bình
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Aedes Albopictus ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated

Bệnh sốt xuất huyết là bệnh truyền nhiễm cấp tính, có thể gây thành dịch lớn và có tỷ lệ tử vong cao. Bệnh lưu hành trên hơn 100 quốc gia trên thế giới ở hầu hết các châu lục. Sốt xuất huyết được lây truyền qua vector trung gian là muỗi Aedes (Aedes aegypti và Aedes albopictus). Việc kiểm soát vector truyền bệnh đóng vai trò quan trọng trong việc ngăn ngừa sự bùng phát của dịch. Trong các biện pháp kiểm soát vector truyền bệnh, việc sử dụng thuốc diệt côn trùng đã mang lại những hiệu quả đáng kể và thuốc diệt côn trùng được sử dụng rộng rãi hiện nay thuộc nhóm pyrethroid. Các hóa chất thuộc nhóm pyrethroid chủ yếu tác động lên côn trùng thông qua các thụ thể trên kênh dẫn truyền Na+ của các neuron thần kinh ở côn trùng. Pyrethroid cản trở sự ngừng hoạt động của kênh dẫn truyền, kết quả là kênh K+ mở trong thời gian dài làm gián đoạn tín hiệu điện trong hệ thống thần kinh, làm mất khả năng bay của côn trùng. Tuy nhiên, khả năng kháng thuốc ở côn trùng trong đó có muỗi đang khiến cho hiệu quả của thuốc diệt côn trùng bị giảm đi. Khả năng kháng thuốc được xác định là do các đột biến trên gen mã hóa cho kênh dẫn truyền Na+ (voltage-gated sodium channel – VGSC). Cho đến nay, rất nhiều đột biến đã được xác định có liên quan đến tính kháng ở các quần thể muỗi Aedes. Thêm vào đó, tần xuất của các đột biến trên gen VGSC ở các quần thể muỗi khác nhau có sự khác biệt rất lớn. Vì vậy, nghiên cứu tính kháng thuốc diệt ở muỗi Aedes sẽ giúp cho việc kiểm soát tốt đối với vector truyền bệnh sốt xuất huyết.

scholarly journals Insecticide resistance of Dengue vectors in South East Asia: a systematic review

2021 ◽  
Vol 21 (3) ◽  
pp. 1124-1140
Author(s):  
Mohd Rohaizat Hassan ◽  
Noor Atika Azit ◽  
Suhaiza Mohd Fadzil ◽  
Siti Rasidah Abd Ghani ◽  
Norfazilah Ahmad ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
East Asia ◽  
Aedes Albopictus ◽  
Pyrethroid Resistance ◽  
Resistance Management ◽  
Genetic Mutation ◽  
Peninsular Malaysia ◽  
South East Asia ◽  
Dengue Vectors

Background: The insecticides used widely has led to resistance in the vector and impose a challenge to vector control op- eration. Objectives: This review aims to analyse the distribution of insecticide resistance of dengue vectors in South East Asia and to describe the mechanism of insecticide resistance. Methods: Literature search for articles published on 2015 to 2019 from PubMed, Scopus and ProQuest was performed. Total of 37 studies included in the final review from the initial 420 studies. Results: Pyrethroid resistance was concentrated on the west coast of Peninsular Malaysia and Northern Thailand and scat- tered at Java Island, Indonesia while organophosphate resistance was seen across the Java Island (Indonesia), West Sumatera and North Peninsular Malaysia. Organochlorine resistance was seen in Sabah, Malaysia and scattered distribution in Nusa Tenggara, Indonesia. V1016G, S989P, F1269C gene mutation in Aedes Aegypti were associated with Pyrethroid resistance in Singapore and Indonesia. In Malaysia, over-expressed with monooxygenase P450 genes (CYP9J27, CYP6CB1, CYP9J26 and CYP9M4) Glutathione S-transferases, carboxylesterases commonly associated with pyrethroids resistance in Aedes Aegypti and CYP612 overexpressed in Aedes Albopictus. The genetic mutation in A302S in Aedes Albopictus was associated with organochlorine resistance in Malaysia. Conclusions: Rotation of insecticide, integration with synergist and routine assessment of resistance profile are recom- mended strategies in insecticide resistance management. Keywords: Insecticide resistance; vector management; Aedes; pyrethroid; mortality.

scholarly journals Frequency of kdr mutations in the voltage-sensitive sodium channel (VSSC) gene in Aedes aegypti from Yogyakarta and implications for Wolbachia-infected mosquito trials

2020 ◽  
Author(s):  
Juli R. Wuliandari ◽  
Ary A. Hoffmann ◽  
Warsito Tantowijoyo ◽  
Nancy M. Endersby-Harshman
Keyword(s):  
Aedes Aegypti ◽  
Inner City ◽  
Insecticide Resistance ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Infected Mosquito ◽  
Simultaneous Occurrence ◽  
Pyrethroid Insecticides ◽  
Background Population ◽  
Homozygous Mutant

Abstract Background : In the inner city of Yogyakarta, Indonesia, insecticide resistance is expected in the main dengue vector, Aedes aegypti , because of the intensive local application of pyrethroid insecticides. However, detailed information about the nature of resistance in this species is required to assist the release of Wolbachia mosquitoes in a dengue control program so that we can ensure that insecticide resistance in the strain of Ae. aegypti being released matches that of the background population. Methods: High-resolution melt genotyping was used to screen for kdr mutations associated with pyrethroid resistance in the voltage-sensitive sodium channel (V SSC ) gene in Ae. aegypti of some areas in the inner city of Yogyakarta. Results: The results show that the V1016G mutation predominated, with individuals homozygous for the 1016G allele at a frequency of 82.1% and the mutant allele G at a frequency of 92%. Two patterns of co-occurrence of mutations were detected in this study, homozygous individuals V1016G/S989P; and heterozygous individuals V1016G/F1534C/S989P. We found the simultaneous occurrence of kdr mutations V1016G and F1534C at all collection sites, but not within individual mosquitoes. Homozygous mutants at locus 1016 were homozygous wildtype at locus 1534 and vice versa, and heterozygous V1016G were also heterozygous for F1534C. The most common tri-locus genotype co-occurrences were homozygous mutant 1016GG and homozygous wild-type FF1534, combined with homozygous mutant 989PP (GG/FF/PP) at a frequency of 38.28%. Conclusions: Given the relatively small differences in frequency of resistance alleles across the city area, locality variations in resistance should have minor implications for the success of Wolbachia mosquito trials being undertaken in the Yogyakarta area.

scholarly journals A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti

2007 ◽  
Vol 16 (6) ◽  
pp. 785-798 ◽  
Author(s):  
K. Saavedra-Rodriguez ◽  
L. Urdaneta-Marquez ◽  
S. Rajatileka ◽  
M. Moulton ◽  
A. E. Flores ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Latin American ◽  
Pyrethroid Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals Individual-based dengue virus surveillance in Aedes aegypti mosquitoes collected concurrently with suspected patients in Tarlac City, Philippines

2020 ◽  
Author(s):  
Jean Claude Balingit ◽  
Thaddeus M. Carvajal ◽  
Mariko Saito-Obata ◽  
Maribet Gamboa ◽  
Amalea Dulcene Nicolasora ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Phylogenetic Analyses ◽  
Simultaneous Detection ◽  
Denv Infection ◽  
Health Concern ◽  
Effective Control ◽  
Entomological Surveillance ◽  
Mosquito Abundance ◽  
Virus Surveillance

Abstract Background Dengue virus (DENV) infection continues to be a major public health concern throughout tropical and subtropical regions of the world where Aedes aegypti mosquitoes, its primary vector, dwell. In the context of DENV transmission, effective control is reliant not only on knowledge of mosquito abundance, but also on mosquito infection. In the 2015 dengue season, we conducted a one-month entomological surveillance of adult Aedes aegypti mosquitoes around households of suspected dengue patients in Tarlac City, Philippines to assess the DENV infection rate in the local mosquito population, and to identify the DENV genotypes and serotypes concurrently co-circulating in mosquitoes and patients. Methods We performed a one-step multiplex real-time RT-PCR assay for the simultaneous detection and serotyping of DENV in patients and in individual female Aedes aegypti mosquito. Consequently, we performed sequencing and phylogenetic analyses to further characterize the detected DENVs in mosquitoes and patients at the genotype level. Results We collected a total of 583 adult Aedes aegypti mosquitoes, of which we tested 359 female mosquitoes individually for the presence of the DENV. Ten mosquitoes (2.8%) from amongst 359 female mosquitoes were confirmed to be positive for the presence of the DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which were consistent with the serotypes concurrently infecting patients. Sequencing and phylogenetic analyses of the detected DENVs based on the partial envelope ( E ) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely: DENV-1 genotype IV, DENV-2 Cosmopolitan genotype and DENV-4 genotype II. Notably, we observed a random geographic distribution of DENVs in the study area suggesting the occurrence of active DENV transmission within and outside the vicinities of Tarlac City. Conclusions In this study, we demonstrate the utility of an individual-based DENV surveillance in field-collected mosquitoes and the importance of incorporating mosquito virus data in phylogenetic studies. Analyzing virus sequences from vector and host could potentially improve our understanding of the dynamics of DENV transmission.

scholarly journals Frequency of kdr mutations in the voltage-sensitive sodium channel (VSSC) gene in Aedes aegypti from Yogyakarta and implications for Wolbachia-infected mosquito trials

2020 ◽  
Author(s):  
Juli R. Wuliandari ◽  
Ary A. Hoffmann ◽  
Warsito Tantowijoyo ◽  
Nancy M. Endersby-Harshman
Keyword(s):  
Aedes Aegypti ◽  
Inner City ◽  
Insecticide Resistance ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Infected Mosquito ◽  
Simultaneous Occurrence ◽  
High Resolution Melt ◽  
Pyrethroid Insecticides ◽  
Homozygous Mutant

Abstract Background: In the inner city of Yogyakarta, Indonesia, insecticide resistance is expected in the main dengue vector, Aedes aegypti, because of the intensive local application of pyrethroid insecticides. However, detailed information about the nature of resistance in this species is required to assist the release of Wolbachia mosquitoes in a dengue control program so that we can ensure that insecticide resistance in the strain of Ae. aegypti being released matches that of the background population.Methods: High-resolution melt genotyping was used to screen for kdr mutations associated with pyrethroid resistance in the voltage-sensitive sodium channel (VSSC) gene in Ae. aegypti of some areas in the inner city of Yogyakarta.Results: The results show that the V1016G mutation predominated, with individuals homozygous for the 1016G allele at a frequency of 82.1% and the mutant allele G at a frequency of 92%. Two patterns of co-occurrence of mutations were detected in this study, homozygous individuals V1016G/S989P; and heterozygous individuals V1016G/F1534C/S989P. We found the simultaneous occurrence of kdr mutations V1016G and F1534C at all collection sites, but not within individual mosquitoes. Homozygous mutants at locus 1016 were homozygous wildtype at locus 1534 and vice versa, and heterozygous V1016G were also heterozygous for F1534C. The most common tri-locus genotype co-occurrences were homozygous mutant 1016GG and homozygous wild-type FF1534, combined with homozygous mutant 989PP (GG/FF/PP) at a frequency of 38.28%.Conclusions: Given the relatively small differences in frequency of resistance alleles across the city area, locality variations in resistance should have minor implications for the success of Wolbachia mosquito trials being undertaken in the Yogyakarta area.

scholarly journals Landscape and Anthropogenic Factors Associated with Adult Aedes aegypti and Aedes albopictus in Small Cities in the Southern Great Plains

Insects ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 699
Author(s):  
Jordan D. Sanders ◽  
Justin L. Talley ◽  
Amy E. Frazier ◽  
Bruce H. Noden
Keyword(s):  
Aedes Aegypti ◽  
Great Plains ◽  
Aedes Albopictus ◽  
The United States ◽  
Health Concern ◽  
Anthropogenic Factors ◽  
Small Cities ◽  
Southern Great Plains ◽  
Factors Associated ◽  
Texas Border

As mosquito-borne diseases are a growing human health concern in the United States, the distribution and potential arbovirus risk from container-breeding Aedes mosquitoes is understudied in the southern Great Plains. The aim of the study was to assess landscape and anthropogenic factors associated with encountering adult container-breeding mosquitoes in small cities in southern Oklahoma. Collections were carried out over a 10 week period from June to August 2017 along two geographical transects, each consisting of three cities, equally distant from the Red River/Texas border. Mosquitoes were collected weekly using two trap types along with data for 13 landscape, vegetation, and anthropogenic variables. After five rounds of collection, 6628 female mosquitoes were collected over 2110 trap-nights involving 242 commercial or residential sites in six cities. Of the mosquitoes collected, 80% consisted of container-breeding species: Aedes albopictus (72%), Culex pipiens complex (16%) and Aedes aegypti (8%). Regionally, Aedes aegypti was more likely present in cities closest to the Texas border while Ae. albopictus was spread throughout the region. In general, Ae. aegypti and Ae. albopictus were significantly more present in sites featuring no or low vegetation and residential sites. Variables associated with Ae. albopictus presence and abundance varied between cities and highlighted the urban nature of the species. The study highlighted the distribution of Ae. aegypti geographically and within the urban context, indicated potential habitat preferences of container-breeding mosquito species in small towns, and demonstrated the usefulness of Gravid Aedes traps (GAT) traps for monitoring Aedes populations in urban habitats in small cities.

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