AhRLK1, a CLAVATA1-like leucine-rich repeat receptor-like kinase of peanut, confers increased resistance to bacterial wilt in tobacco

AbstractBacterial wilt caused by Ralstonia solanacearum is a devastating disease that infects hundreds of plant species. Host factors involved in disease resistance and pathogenesis remain poorly characterized. An up regulated and leucine-rich repeat receptor-like kinase characterized as CLAVATA1 and named AhRLK1 was obtained by microarray analysis in response to R. solanacearum in peanut. AhRLK1 contained presumably, a signal peptide, ten leucine-rich repeat (LRR) domains and conserved motifs of intracellular kinases. For subcellular localization, the AhRLK1 protein was visualized only in the plasma membrane. After inoculation with R. solanacearum, AhRLK1 was constantly up regulated in the susceptible variety Xinhuixiaoli but showed little changed in the resistant cultivar Yueyou92. Different hormones, including salicylic acid, abscisic acid, methyl jasmonate, and ethephon, induced expression, but expression was completely down regulated under cold and drought treatments. Transient overexpression provoked a hypersensitive response (HR) in Nicotiana benthamiana following agro-infiltration. Furthermore, in transgenic tobacco with overexpression of the gene, the resistance to R. solanacearum increased significantly. By contrast, most representative defense-responsive genes in HR, SA, JA and ET signal pathways such as NtHIN1, NtPR2, NtLOX1, and NtACS6, among others, were considerably up regulated in the AhRLK1 transgenic lines. Additionally, the EDS1 and PAD4 in the R gene signal were also up regulated in transgenic plants, but the NDR1 and NPR1 genes were down regulated. Accordingly, we suggest that AhRLK1 increases the defense response to R. solanacearum via HR and hormone defense signalling, associated with the EDS1 pathway of R gene signalling. The results provide new understanding of CLV1 function and will contribute to genetic enhancement of peanut.

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Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco

Journal of Experimental Botany ◽

10.1093/jxb/erz274 ◽

2019 ◽

Vol 70 (19) ◽

pp. 5407-5421

Author(s):

Chong Zhang ◽

Hua Chen ◽

Rui-Rong Zhuang ◽

Yu-Ting Chen ◽

Ye Deng ◽

...

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Defense Response ◽

R Gene ◽

Leucine Rich Repeat ◽

Receptor Like Kinase ◽

Defense Response Signaling ◽

Resistance To Ralstonia Solanacearum

Overexpression of a novel peanut CLAVATA1-like gene significantly enhanced the resistance to Ralstonia solanacearum in tobacco via defense response signaling associated with EDS1-mediated R-gene pathways.

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Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera

10.1101/2020.02.18.954479 ◽

2020 ◽

Cited By ~ 2

Author(s):

Ulrich Hohmann ◽

Priya Ramakrishna ◽

Kai Wang ◽

Laura Lorenzo-Orts ◽

Joel Nicolet ◽

...

Keyword(s):

Receptor Activation ◽

Activation Mechanism ◽

Signaling Proteins ◽

Receptor Kinase ◽

Leucine Rich Repeat ◽

Protein Ligands ◽

Receptor Like Kinase ◽

Transgenic Lines ◽

Receptor Kinase Signaling ◽

Membrane Signaling

AbstractReceptor kinases with extracellular leucine-rich repeat domains (LRR-RKs) form the largest group of membrane signaling proteins in plants. LRR-RKs can sense small molecule, peptide or protein ligands, and may be activated by ligand-induced interaction with a shape complementary SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptor kinase. We have previously shown that SERKs can also form constitutive, ligand-independent complexes with the LRR ectodomains of BAK1-interacting receptor-like kinase 3 (BIR3) receptor pseudokinases, negative regulators of LRR-RK signaling. Here we report that receptor chimaera in which the extracellular LRR domain of BIR3 is fused to the cytoplasmic kinase domains of the SERK-dependent LRR-RKs BRASSINOSTEROID INSENSITIVE1, HAESA and ERECTA form tight complexes with endogenous SERK co-receptors in the absence of ligand stimulus. Expression of these chimaera under the control of the endogenous promoter of the respective LRR-RK leads to strong gain-of-function brassinosteroid, floral abscission and stomatal patterning phenotypes, respectively. Importantly, a BIR3-GSO1/SGN3 chimera can partially complement sgn3 Casparian strip formation phenotypes, suggesting that GSO1/SGN3 receptor activation is also mediated by SERK proteins. Collectively, our protein engineering approach may be used to elucidate the physiological functions of orphan LRR-RKs and to identify their receptor activation mechanism in single transgenic lines.

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TaXa21, a Leucine-Rich Repeat Receptor–Like Kinase Gene Associated with TaWRKY76 and TaWRKY62, Plays Positive Roles in Wheat High-Temperature Seedling Plant Resistance to Puccinia striiformis f. sp. tritici

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-05-19-0137-r ◽

2019 ◽

Vol 32 (11) ◽

pp. 1526-1535 ◽

Cited By ~ 8

Author(s):

Jiahui Wang ◽

Junjuan Wang ◽

Hongsheng Shang ◽

Xianming Chen ◽

Xiangming Xu ◽

...

Keyword(s):

High Temperature ◽

Puccinia Striiformis ◽

Kinase Domain ◽

Signal Pathways ◽

Leucine Rich Repeat ◽

Virus Induced Gene Silencing ◽

Kinase Gene ◽

Rice Bacterial Blight ◽

Receptor Like Kinase ◽

Seedling Plant

Puccinia striiformis f. sp. tritici causes wheat stripe rust, one of most important diseases of wheat worldwide. High-temperature seedling plant (HTSP) resistance of wheat to P. striiformis f. sp. tritici is one specific type of host resistance, induced by high temperature (HT). Receptor-like kinases (RLKs) play key roles in regulating plant development and signaling networks, but there have been no reports on possible roles played by RLKs in wheat HTSP to P. striiformis f. sp. tritici. In the present study, a leucine rich repeat (LRR)-RLK gene, TaXa21, with a high homology with rice bacterial blight resistance gene Xa21, was cloned from wheat cultivar Xiaoyan 6 (XY 6). TaXa21 expression was up-regulated by the exposure to HT (20°C) for 24 h at 8 days postinoculation with P. striiformis f. sp. tritici and was induced by ethylene (ET) and hydrogen peroxide (H2O2). Knocking down TaXa21 using virus-induced gene silencing reduced HTSP resistance to P. striiformis f. sp. tritici compared with the control plants. In addition, the expression level of TaCAT in the H2O2 pathway was induced and TaACO in the ET signal pathway was reduced in the HT-treated TaXa21-silenced plants. Transient expression of TaXa21 in tobacco leaves confirmed its subcellular localization in plasma membrane, consistent with the prediction from bioinformatics analysis. The transmembrane and kinase domain of TaXa21 can interact with TaWRKY76 in the nucleus and cell membrane, which is different from the localization of Xa21 in rice. The interaction between TaWRKY76 and TaWRKY62 (positively involved in the HTSP resistance of XY 6) were observed. Together, these results indicated that TaXa21 is a RLK associated with TaWRKY76 and TaWRKY62 and functions as a positive regulator of wheat HTSP resistance to P. striiformis f. sp. tritici. Furthermore, the host defense is mediated by the H2O2 and ET signal pathways.

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Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2016.1242377 ◽

2016 ◽

Vol 31 (1) ◽

pp. 51-57 ◽

Cited By ~ 5

Author(s):

Yongrong Liao ◽

Changqiong Hu ◽

Xuewei Zhang ◽

Xufeng Cao ◽

Zhengjun Xu ◽

...

Keyword(s):

Abiotic Stress ◽

Stress Responses ◽

Leucine Rich Repeat ◽

Receptor Like Kinase

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Molecular characterization and functional analysis of CzR1, a coiled-coil-nucleotide-binding-site-leucine-rich repeat R-gene from Curcuma zedoaria Loeb. that confers resistance to Pythium aphanidermatum

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2013.05.003 ◽

2013 ◽

Vol 83 ◽

pp. 59-68 ◽

Cited By ~ 13

Author(s):

Basudeba Kar ◽

Satyabrata Nanda ◽

Pradeep Kumar Nayak ◽

Sanghamitra Nayak ◽

Raj Kumar Joshi

Keyword(s):

Functional Analysis ◽

Binding Site ◽

Molecular Characterization ◽

Coiled Coil ◽

Nucleotide Binding ◽

Pythium Aphanidermatum ◽

Nucleotide Binding Site ◽

R Gene ◽

Leucine Rich Repeat ◽

Curcuma Zedoaria

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Biovar Differentiation and Variation in Virulence of Ralstonia solanacearum Isolates Infecting Solanaceous Vegetables

Journal of Nepal Agricultural Research Council ◽

10.3126/jnarc.v2i0.16117 ◽

2016 ◽

Vol 2 ◽

pp. 22-26 ◽

Cited By ~ 1

Author(s):

Ram Devi Timila ◽

Shrinkhala Manandhar

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Negative Impact ◽

Virulent Strain ◽

Solanum Melongena ◽

Susceptible Variety ◽

Resistant Variety ◽

Slight Variation ◽

Solanaceae Family ◽

Moderately Resistant

Bacterial wilt caused by Ralstonia solanacearum E.F. Smith is one of the destructive diseases of solanaceous vegetables specially tomato (Lycopersicon esculentum L.) and eggplant (Solanum melongena L.). Experiments were conducted to determine biovar types existing among the strains or isolates of Nepal and variation in virulence in some vegetables belonging to solanaceae family. A total of 39 isolates infecting tomato, eggplant, chilli and potato collected from different parts of Nepal were analyzed for biovar types on the basis of 3 disaccharides and 3 hexose alcohols oxidation test. Experiments were conducted to determine variation in virulence or aggressiveness of some of the isolates under screen house conditions using three host differentials such as Pusa Ruby (susceptible), Bishesh (moderately resistant) and Srijana (resistant) tomato cultivars. Of the 39 isolates, 23 were biovar III, three biovar II, three biovar IV, and one was biovar I. Nine isolates could not be differentiated into any of the five biovars. For breeding and epidemiological purposes it is very important to analyze the variability of aggressiveness. A total of 5 isolates collected from different places were included in the test. Isolates from Bhaktapur was found the most virulent causing wilt in the variety Bishesh (moderately resistant). Other isolates had the negative impact with zero wilt on the differentials used. Isolates from Jungekhola of Dhading district did not induce wilt even on susceptible variety (Pusa Ruby), but exhibited only senescence reaction. The result indicated that there is some slight variation among the isolates tested. Some effective management tactics might be needed in those locations where highly aggressive or virulent strain of bacterial wilt is prevalent, because resistant variety may not be stable in such locations.

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