scholarly journals Comprehensive analysis of miRNA and protein profiles within exosomes derived from canine lymphoid tumour cell lines

2018 ◽  
Author(s):  
Hajime Asada ◽  
Hirotaka Tomiyasu ◽  
Takao Uchikai ◽  
Genki Ishihara ◽  
Yuko Goto-Koshino ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Cell Communication ◽  
Cell Types ◽  
Resistant Cell ◽  
Comprehensive Analysis ◽  
Protein Profiles ◽  
Tumour Cell Lines ◽  
Almost All ◽  
Lymphoid Tumour

AbstractExosomes are small extracellular vesicles released from almost all cell types, which play roles in cell-cell communication. Recent studies have suggested that microenvironmental crosstalk mediated by exosomes is an important factor in the escape of tumour cells from the anti-tumour immune system in human haematopoietic malignancies. Here, we conducted comprehensive analysis of the miRNA and protein profiles within the exosomes released from four canine lymphoid tumour cell lines as a model of human lymphoid tumours. The results showed that the miRNAs and proteins abundantly contained in exosomes were similar among the four cell lines. However, the profiles of miRNA within exosomes differed among the cell lines and reflected the expression pattern of miRNAs of the parent cells. In the comparison of the amounts of miRNAs and proteins among the cell lines, those of three miRNAs (miR-151, miR-8908a-3p, and miR-486) and CD82 protein differed between exosomes derived from vincristine-sensitive and resistant cell lines. Further investigations are needed to elucidate the biological functions of the exosomal contents in the microenvironmental crosstalk of lymphoid tumours.

scholarly journals Comprehensive analysis of miRNA and protein profiles within exosomes derived from canine lymphoid tumour cell lines

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0208567 ◽  
Author(s):  
Hajime Asada ◽  
Hirotaka Tomiyasu ◽  
Takao Uchikai ◽  
Genki Ishihara ◽  
Yuko Goto-Koshino ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Comprehensive Analysis ◽  
Protein Profiles ◽  
Tumour Cell Lines ◽  
Lymphoid Tumour

scholarly journals Characterization of difluoromethylornithine-resistant mouse and human tumour cell lines

1989 ◽  
Vol 258 (3) ◽  
pp. 709-713 ◽  
Author(s):  
A Hirvonen ◽  
T Eloranta ◽  
T Hyvönen ◽  
L Alhonen ◽  
J Jänne
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Tumour Cell ◽  
Resistant Cell ◽  
Human Cell Lines ◽  
Human Tumour ◽  
Resistant Mouse ◽  
Tumour Cell Lines ◽  
Human Tumour Cell Lines

Four mouse and two human tumour cell lines resistant to alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), were analysed for the activities of polyamine-biosynthetic and -biodegradative enzymes as well as for cellular polyamine contents. In all but one of these cell lines the resistance to DFMO was based on an overproduction of ODC. In a human myeloma cell line the resistance was based on a greatly enhanced arginase activity. Except for one L1210 variant cell line, all the resistant cell lines contained elevated S-adenosylmethionine decarboxylase activity. Similarly, all the resistant mouse, but not human, cell lines displayed enhanced spermidine and spermine synthase activities. Arginase activity was detected only in human cell lines. In both DFMO-resistant cell lines the activity of arginase was strikingly elevated. Of the biodegradative enzymes, polyamine oxidase activity was readily detectable in all mouse cells, but no measurable activity was found in the human cells. Spermidine/spermine N1-acetyltransferase activity was elevated in three out of four resistant mouse cell lines. Even though the concentration of spermidine was usually lower in the overproducer cells, this was compensated by an increased content of spermine. The two resistant human myeloma cells contained intracellular ornithine concentrations that were from more than 5 to more than 20 times higher than those in the parental cells.

Reading between the lines: molecular characterization of five widely used canine lymphoid tumour cell lines

2011 ◽  
Vol 11 (1) ◽  
pp. 30-50 ◽  
Author(s):  
E. L. Seiser ◽  
R. Thomas ◽  
K. L. Richards ◽  
M. Kathryn Kelley ◽  
P. Moore ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cell Lines ◽  
Tumour Cell ◽  
Tumour Cell Lines ◽  
Lymphoid Tumour

In Vitro Immunogenicity of Human Lymphoid Tumour Cell Lines

1985 ◽  
pp. 553-561
Author(s):  
D. J. Moss ◽  
J. A. Staples ◽  
S. R. Burrows ◽  
J. Ryan ◽  
T. B. Sculley
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Tumour Cell Lines ◽  
Lymphoid Tumour

Antitumour effect and modulation of expression of the ABCB1 gene by perifosine in canine lymphoid tumour cell lines

2014 ◽  
Vol 201 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Hirotaka Tomiyasu ◽  
Yuko Goto-Koshino ◽  
Yasuhito Fujino ◽  
Koichi Ohno ◽  
Hajime Tsujimoto
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Antitumour Effect ◽  
Abcb1 Gene ◽  
Tumour Cell Lines ◽  
Lymphoid Tumour

Epigenetic regulation of the ABCB1 gene in drug-sensitive and drug-resistant lymphoid tumour cell lines obtained from canine patients

2014 ◽  
Vol 199 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Hirotaka Tomiyasu ◽  
Yuko Goto-Koshino ◽  
Yasuhito Fujino ◽  
Koichi Ohno ◽  
Hajime Tsujimoto
Keyword(s):  
Cell Lines ◽  
Epigenetic Regulation ◽  
Tumour Cell ◽  
Drug Resistant ◽  
Abcb1 Gene ◽  
Tumour Cell Lines ◽  
Lymphoid Tumour

scholarly journals The main neutral aminopeptidase activity of human lymphoid tumour cell lines does not originate from the aminopeptidase N-(APN; CD13) gene

1997 ◽  
Vol 1355 (2) ◽  
pp. 147-154 ◽  
Author(s):  
T Wex ◽  
U Lendeckel ◽  
T Kähne ◽  
A Ittenson ◽  
K Frank ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Aminopeptidase N ◽  
Aminopeptidase Activity ◽  
Tumour Cell Lines ◽  
Lymphoid Tumour

Screening of Venezuelan medicinal plant extracts for cytostatic and cytotoxic activity against tumour cell lines

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
P Taylor ◽  
M Arsenak ◽  
MJ Abad ◽  
Á Fernández ◽  
R Gonto ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Plant Extracts ◽  
Tumour Cell ◽  
Medicinal Plant Extracts ◽  
Tumour Cell Lines

Cytotoxic activity against tumour cell lines and anti-inflammatory effects of compounds isolated from Xylopia aromatica

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
O Estrada ◽  
L González ◽  
M Mijares ◽  
Á Fernández ◽  
M Ruiz ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Tumour Cell ◽  
Tumour Cell Lines ◽  
Anti Inflammatory

Epigenetic modifiers reduce proliferation of human neuroendocrine tumour cell lines

2013 ◽  
pp. 1-1
Author(s):  
E Kate Lines ◽  
U Katherine Gaynor ◽  
Mark Stevenson ◽  
J Paul Newey ◽  
E Sian Piret ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Neuroendocrine Tumour ◽  
Epigenetic Modifiers ◽  
Tumour Cell Lines
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