scholarly journals Construction of a genetic linkage map in Pyropia yezoensis (Bangiales, Rhodophyta) and QTL analysis of several economic traits of blades

2018 ◽  
Author(s):  
linbin huang ◽  
Xing-hong Yan
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Specific Growth ◽  
Pyropia Yezoensis ◽  
Economic Traits ◽  
Fresh Weight ◽  
Skewed Segregation

Pyropia yezoensis is an important economic seaweed, to construct a genetic linkage map and analyze the quantitative trait loci (QTLs) of blades, a doubled haploid (DH) population containing 148 DH strains established from the intraspecific hybridization between two strains with different colors was used in the present study and genotyped using 79 pairs of polymorphic sequence-related amplified polymorphism (SRAP) markers labeled with 5'-HEX and capillary electrophoresis. A chi-square test for significance of deviations from the expected ratio (1:1) on the loci which were polymorphic between parents and segregated in mapping population identified 301 loci with normal segregation (P ≥ 0.01) and 96 loci (24.18%) with low-level skewed segregation (0.001 ≤ P < 0.01). The map was constructed using JoinMap software after a total of 92 loci were assembled into three linkage groups. The map spanned 557.36 cM covering 93.71% of the estimated genome, with a mean interlocus space of 6.23 cM. Kolmogorov-Smirnov test (α=5%) of the marker positions along each LG showed a uniform distribution. After that, 10 QTLs associated with five economic traits of blades were detected, among which one QTL was for length, one for width, two for fresh weight, two for specific growth rate of length and four for specific growth rate of fresh weight. These QTLs could explain 2.29-7.87% of the trait variations, indicating that their effects were all minor. The results will serve as a framework for future marker-assisted breeding in P. yezoensis.

A fine‐scale genetic linkage map reveals genomic regions associated with economic traits in walnut ( Juglans regia )

2019 ◽  
Vol 138 (5) ◽  
pp. 635-646 ◽  
Author(s):  
Mallikarjuna K. Aradhya ◽  
Dianne Velasco ◽  
Ji‐Rui Wang ◽  
Ramesh Ramasamy ◽  
Frank M. You ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Juglans Regia ◽  
Fine Scale ◽  
Economic Traits ◽  
Genomic Regions

scholarly journals DEVELOPING GENETIC LINKAGE MAP AND CDNA SUBTRACTION LIBRARY FOR WATERMELON

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 871e-872
Author(s):  
A. Levi ◽  
C. E. Thomas ◽  
A. Davis ◽  
O.U.K. Reddy ◽  
Y. Xu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Medium Size ◽  
Local Alignment ◽  
Maturation Stage ◽  
Cdna Clones ◽  
Linkage Groups ◽  
F2 Population ◽  
Skewed Segregation

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. The testcross map comprises 262 markers (RAPD, ISSR, AFLP, SSR and ASRP markers) and covers 1,350 cM. The map comprises 11 large linkage groups (50.7–155.2 cM), 5 medium-size linkage groups (37.5–46.2 cM), and 16 small linkage groups (4.2–31.4 cM). Most AFLP markers are clustered on two linkage regions, while all other marker types are randomly dispersed on the genome. Many of the markers in this study are skewed from the classical (Mendelian) segregation ratio of1:1 in the testcross or the 3:1 ratio in the F2 population. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and F2 population. A cDNA library was constructed using RNA isolated from watermelon flesh 1 week (rapid cell division stage), 2 weeks (cell growth and storage deposition stage, 4 weeks (maturation stage), and 5 weeks (postmaturation stage) post pollination. Over 1,020 cDNA clones were sequenced, and were analyzed using the Basic Local Alignment Search Tool (BLAST). The sequenced cDNA clones were designated as expressed sequenced tag (EST) markers and will be used in mapping analysis of watermelon genome.

scholarly journals GENETIC LINKAGE MAP FOR WATERMELON: SEGREGATION AND DISTRIBUTION OF DNA MARKERS

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 872a-872
Author(s):  
A. Levi ◽  
C. E. Thomas ◽  
J. Thies ◽  
A. Simmons ◽  
Y. Xu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Genetic Distances ◽  
Aflp Markers ◽  
F2 Population ◽  
Skewed Segregation ◽  
Linkage Distance ◽  
Map Distance

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. About 51.0% and 31.8% of the markers in the testcross and F2 populations are skewed form the expected segregation ratios. AFLP markers appeared to be clustered on linkage regions, while ISSR and RAPD markers are randomly dispersed on the genome. AFLP markers also have greater genetic distances as compared with ISSR and RAPD markers, resulting in significant increase of map distance. An initial genetic map (based on the testcross population) that contains 27 ISSR and 141 RAPD markers has a total linkage distance of 1,166.2 cM. The addition of 2 ISSR, 8 RAPD and 77 AFLP markers increased the genetic distance of the map to 2,509.9 cM. Similar results with AFLP markers were also shown in mapping experiments with an F2S7 recombinant inbred line (RIL) population that was recently constructed for watermelon. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and the F2 population. Experiments with SSR, and EST markers are being conducted to saturate the linkage map of watermelon genome.

The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis

2009 ◽  
Vol 31 (6) ◽  
pp. 629-637 ◽  
Author(s):  
Wei-Dong LIU ◽  
Xiang-Bo BAO ◽  
Wen-Tao SONG ◽  
Zun-Chun ZHOU ◽  
Chong-Bo HE ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Japanese Scallop ◽  
Mizuhopecten Yessoensis
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