scholarly journals Effect of flow rate and freezing on cyanocobalamin recovery using a commercial solid phase extraction cartridge

2019 ◽  
Author(s):  
Lauren Lees ◽  
Alison M. Bland ◽  
Giacomo R. DiTullio ◽  
Michael G. Janech ◽  
Peter A. Lee
Keyword(s):  
Solid Phase Extraction ◽  
Vitamin B12 ◽  
Flow Rate ◽  
Sea Water ◽  
Solid Phase ◽  
Negative Impact ◽  
Field Studies ◽  
Phase Extraction ◽  
Flow Rates ◽  
Solid Phase Extraction Cartridge

AbstractAnalysis of vitamin B12 in sea water is laborious, time consuming, and often requires storage of relatively large-volume water samples. Alleviating these major limitations will increase the throughput of samples and, as a consequence, improve our understanding of the distribution and role of vitamin B12 in the oceans. Previous studies have indicated that target analyte recovery is negatively affected at flow rates exceeding 1 mL min−1 using home-made C18 Solid Phase Extraction (SPE) cartridges. In this study, the effect of flow rate on recovery of vitamin B12 was tested across a range of flow rates between 1 and 37 mL min−1 using a commercial SPE cartridge containing surface-modified styrene divinylbenzene. Recovery of vitamin B12 at flow rates up to the maximum rate tested did not statistically differ from 1 mL min 1. A second study was conducted to determine whether storage of the SPE cartridges at −20°C had a negative impact on vitamin B12 recovery. Recovery of vitamin B12 from SPE cartridges stored up to 13 days did not differ from unfrozen SPE cartridges. These data suggest that rapid extraction and cold storage of vitamin B12 on commercial SPE cartridges does not negatively affect recovery and offers an economical alternative to field studies.

scholarly journals Effect of flow rate and freezing on cyanocobalamin recovery using a commercial solid phase extraction cartridge

2020 ◽  
pp. 014-018
Author(s):  
Lees Lauren ◽  
Bland Alison M ◽  
DiTullio Giacomo R ◽  
Janech Michael G ◽  
Lee Peter A
Keyword(s):  
Solid Phase Extraction ◽  
Flow Rate ◽  
Solid Phase ◽  
Phase Extraction ◽  
Solid Phase Extraction Cartridge

Gas Chromatographic/Mass Spectrometric Confirmation of Leucomalachite Green in Catfish (Ictalurus punctatus) Tissue

1995 ◽  
Vol 78 (4) ◽  
pp. 971-977 ◽  
Author(s):  
Sherri B Turnipseed ◽  
José E Roybal ◽  
Jeffrey A Hurlbut ◽  
Austin R Long
Keyword(s):  
Solid Phase Extraction ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Mass Spectrometric ◽  
Phase Extraction ◽  
Neutral Alumina ◽  
Ms Analysis ◽  
Solid Phase Extraction Cartridge ◽  
Leucomalachite Green ◽  
Chromatographic Mass

Abstract A gas chromatographic/mass spectrometric (GC/MS) method was developed to confirm the presence of leucomalachite green (LMG), a metabolite of the triphenylmethane dye malachite green (MG), in catfish tissue. Residues were isolated according to a previously described liquid chromatographic (LC)A/IS spectrometric analysis of MG and LMG in fish. In our isolation procedure, analytes are extracted from tissue with acetonitrile–buffer, partitioned into CH2CI2, and applied to neutral alumina and propylsulfonic acid solid-phase extraction cartridges. Before GC/MS analysis, extracts prepared for the LC determinative method are eluted from a cyano solid-phase extraction cartridge, extracted into organic solvent, and concentrated for GC/MS analysis. Selected ion monitoring was performed by using 5 diagnostic ions (m/z 330,329,253,210, and 165) of LMG. The method was validated by confirming LMG in tissue fortified with mixtures of MG and LMG (5 and 10 ng/g each) and in tissue from fish that had been exposed to low levels of MG.

A simple and efficient automated cGMP-compliant radiosynthesis of [11 C]metomidate using solid phase extraction cartridge purification

2019 ◽  
Vol 62 (4) ◽  
pp. 190-197 ◽  
Author(s):  
Mitja Kovac ◽  
Tunde Miklovicz ◽  
Lei Li ◽  
Joseph Russell ◽  
Roberto Canales Candela ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Solid Phase Extraction Cartridge

scholarly journals Determination of Low Levels of Lead in Beer Using Solid-Phase Extraction and Detection by Flame Atomic Absorption Spectrometry

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Vanessa N. Alves ◽  
Simone S. O. Borges ◽  
Waldomiro B. Neto ◽  
Nívia M. M. Coelho
Keyword(s):  
Solid Phase Extraction ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flow Rate ◽  
Flame Atomic Absorption Spectrometry ◽  
Solid Phase ◽  
Absorption Spectrometry ◽  
Phase Extraction ◽  
Flame Atomic Absorption

In this study, a method for the determination of low concentrations of lead in beer samples using solid-phase extraction with a flow injection analysis system and detection by flame atomic absorption spectrometry (FAAS) was developed.Moringa oleiferaseeds were used as a biosorbent material. Chemical and flow variables of the online preconcentration system, such as sample pH, preconcentration flow rate, eluent flow rate, eluent concentration, particle size, and sorbent mass, were studied. The optimum extraction conditions were obtained using a sample pH of 6.0, sample flow rate of 6.0 mL min−1, 63.0 mg of sorbent mass, and 2.0 mol L−1HNO3at a flow rate of 2.0 mL min−1as the eluent. With the optimized conditions, the preconcentration factor, precision, detection limit, consumption index, and sample throughput were estimated as 93, 0.3% (10.0 μg L−1,n=7), 7.5 μg L−1, 0.11 mL, and 23 samples per hour, respectively. The method developed was successfully applied to beer samples and recovery tests, with recovery ranging from 80% to 100%.

Development of an on-line solid phase extraction method based on new functionalized magnetic nanoparticles. Use in the determination of mercury in biological and sea-water samples

Talanta ◽  
2016 ◽  
Vol 153 ◽  
pp. 228-239 ◽  
Author(s):  
Elisa Vereda Alonso ◽  
M.M. López Guerrero ◽  
Pilar Colorado Cueto ◽  
José Barreno Benítez ◽  
José Manuel Cano Pavón ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Solid Phase Extraction ◽  
Water Samples ◽  
Extraction Method ◽  
Sea Water ◽  
Solid Phase ◽  
Phase Extraction ◽  
Functionalized Magnetic Nanoparticles ◽  
On Line
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