scholarly journals An environmental DNA tool for monitoring the status of the Critically Endangered Smalltooth Sawfish, Pristis pectinata, in the Western Atlantic

2019 ◽  
Author(s):  
Ryan N. Lehman ◽  
Gregg R. Poulakis ◽  
Rachel M. Scharer ◽  
Katherine E. Schweiss ◽  
Jill M. Hendon ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Critically Endangered ◽  
Ex Situ ◽  
Past Century ◽  
Western Atlantic ◽  
The Core ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Target Dna ◽  
The Status ◽  
Pristis Pectinata

AbstractThe Critically Endangered Smalltooth Sawfish, Pristis pectinata, was once widespread in the tropical and subtropical waters of the Atlantic Ocean, but following substantial declines over the past century, the core population is currently confined to southwest Florida in the U.S. and the Bahamas. Recent research and verified public encounter reports suggests that this core population may be stabilizing and, potentially, expanding into formerly occupied areas of their historic range in the Western Atlantic; however, the status of this species in non-core waters is not well understood. Environmental DNA (eDNA) methods provide a relatively cost effective and rapid assessment tool for monitoring species occurrence in aquatic habitats. Here, we have developed an eDNA tool: a species-specific Droplet Digital™ PCR (ddPCR™) assay targeting a 100-base pair portion of the mitochondrial NADH dehydrogenase subunit 2 gene in P. pectinata, with the ability to reliably detect as little as 0.25 pg of target DNA. The assay was validated by collecting and analyzing a water sample from known P. pectinata nursery habitat in Florida, which was found to contain an average of 11.54 copies of target DNA/µL (SE = 0.72) in the reaction. The assay was then further tested by placing a juvenile sawfish in an ex situ tank and analyzing water samples collected at time intervals. The implementation of this eDNA tool into field surveys will provide additional, reliable data to assess species recovery and aid in prioritizing localities beyond the core range in which to focus research and education initiatives.

scholarly journals An environmental DNA tool for monitoring the status of the Critically Endangered Smalltooth Sawfish, Pristis pectinata, in the western Atlantic

2020 ◽  
Vol 12 (4) ◽  
pp. 621-629
Author(s):  
Ryan N. Lehman ◽  
Gregg R. Poulakis ◽  
Rachel M. Scharer ◽  
Katherine E. Schweiss ◽  
Jill M. Hendon ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Critically Endangered ◽  
Western Atlantic ◽  
The Status ◽  
Pristis Pectinata

Conservation of the critically endangered Achondrostoma occidentale (Pisces: Cyprinidae): integrating habitat data in restocking programs to improve population recovery

Gaia Scientia ◽  
2016 ◽  
Vol 10 (2) ◽  
pp. 1-9
Author(s):  
D. Mameri ◽  
C. Sousa Santos ◽  
M. F. Magalhães ◽  
J. I. Robalo
Keyword(s):  
Iberian Peninsula ◽  
River Basins ◽  
Fish Abundance ◽  
Critically Endangered ◽  
Ex Situ ◽  
Population Recovery ◽  
Age Classes ◽  
Younger Age ◽  
Risk Of Extinction

Cyprinid fauna endemic to the Iberian Peninsula is currently facing several human-caused threats which enhance their risk of extinction. The populations of the critically endangered Achondrostoma occidentale, occurring only in three small and intermittent Portuguese river basins, have been reinforced with fish bred ex-situ in order to minimize their risk of extinction. This study aimed to describe the restocking actions conducted and to identify and characterize the summer refugia that should become targets for restoration and further restocking. The results evidence population increments and a high representativeness of younger age classes in the restocked populations. The importance of annual monitoring of fish abundance to assess how the species is coping with the stochastic and deterministic stressors that may eventually compromise its survival is highlighted.

scholarly journals In the midst of theory and practice: a foreword

2020 ◽  
pp. 1-5
Author(s):  
Hannes Peltonen ◽  
Knut Traisbach
Keyword(s):  
Social World ◽  
World Society ◽  
Theory And Practice ◽  
Pragmatic Approach ◽  
The Core ◽  
Critical Engagement ◽  
The Social ◽  
The Status

Abstract This foreword frames the Symposium in two ways. It summarises the core themes running through the nine ‘meditations’ in The Status of Law in World Society. Moreover, it places these themes in the wider context of Kratochwil's critical engagement with how we pursue knowledge of and in the social world and translate this knowledge into action. Ultimately, also his pragmatic approach cannot escape the tensions between theory and practice. Instead, we are in the midst of both.

scholarly journals Status of the Ex Situ and In Situ Conservation of Brazilian Crop Wild Relatives of Rice, Potato, Sweet Potato, and Finger Millet: Filling the Gaps of Germplasm Collections

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 638
Author(s):  
Marcelo B. Medeiros ◽  
José F. M. Valls ◽  
Aluana G. Abreu ◽  
Gustavo Heiden ◽  
Suelma Ribeiro-Silva ◽  
...  
Keyword(s):  
Finger Millet ◽  
Ex Situ Conservation ◽  
Gap Analysis ◽  
In Situ Conservation ◽  
Germplasm Collection ◽  
Crop Wild Relatives ◽  
Wild Relatives ◽  
Ex Situ ◽  
The Status

This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O’Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa’s ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country.

The Receding Animal: Theorizing Anxiety and Attachment in Psychoanalysis from Freud to Imre Hermann

2016 ◽  
Vol 29 (1) ◽  
pp. 55-76 ◽  
Author(s):  
Lydia Marinelli ◽  
Andreas Mayer
Keyword(s):  
Oedipus Complex ◽  
Death Instinct ◽  
Case Histories ◽  
The Core ◽  
The Status ◽  
Animal Phobias ◽  
Indirect Observation ◽  
To Come ◽  
Wolf Man ◽  
Little Hans

ArgumentAnimals played an important role in the formation of psychoanalysis as a theoretical and therapeutic enterprise. They are at the core of texts such as Freud's famous case histories of Little Hans, the Rat Man, or the Wolf Man. The infantile anxiety triggered by animals provided the essential link between the psychology of individual neuroses and the ambivalent status of the “totem” animal in so-called primitive societies in Freud's attempt to construct an anthropological basis for the Oedipus complex in Totem and Taboo. In the following, we attempt to track the status of animals as objects of indirect observation as they appear in Freud's classical texts, and in later revisionist accounts such as Otto Rank's Trauma of Birth and Imre Hermann's work on the clinging instinct. In the 1920s and 1930s, the Freudian conception of patients' animal phobias is substantially revised within Hermann's original psychoanalytic theory of instincts which draws heavily upon ethological observations of primates. Although such a reformulation remains grounded in the idea of “archaic” animal models for human development, it allows to a certain extent to empiricize the speculative elements of Freud's later instinct theory (notably the death instinct) and to come to a more embodied account of psychoanalytic practice.

scholarly journals Territorial organization of the restaurant facilities of Kyiv agglomeration

2015 ◽  
pp. 79-82
Author(s):  
K. Dmytriieva
Keyword(s):  
Restaurant Industry ◽  
The Core ◽  
Industry Standards ◽  
The Status ◽  
Territorial Organization ◽  
The Difference ◽  
Surrounding Areas ◽  
Concentration Ratios

The article outlines the factors of development of the restaurant facilities in Kyiv and surrounding areas. The status of the territorial organization of the restaurant facilities of Kyiv and Kyiv region through the review of the main indicators of the industry by districts in 2013 has been analyzed. Comparison of the industry standards due to availability of these services to population needs has been performed. The result of the difference of efficiency of the restaurant industry through calculation of concentration ratios turnover has been received. Area that can be considered as the core of the restaurant industry of Kyiv agglomeration and area with the potential of growth has been identified. The article visualized by maps.

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