scholarly journals Constitutively enhanced genome integrity maintenance and direct stress mitigation characterize transcriptome of extreme stress-adapted Arabidopsis halleri

2019 ◽  
Author(s):  
Gwonjin Lee ◽  
Julia Quintana ◽  
Hassan Ahmadi ◽  
Lara Syllwasschy ◽  
Nadežda Janina ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Functional Class ◽  
Genome Integrity ◽  
Arabidopsis Halleri ◽  
Natural Habitats ◽  
Long Terminal Repeat Retrotransposon ◽  
Extreme Stress ◽  
Protein Encoding ◽  
Somatic Genome ◽  
Filament Protein

AbstractHeavy metal-rich toxic soils and ordinary soils are both natural habitats of Arabidopsis halleri. The molecular divergence underlying survival in sharply contrasting environments is unknown. Here we comparatively address metal physiology and transcriptomes of A. halleri originating from the most highly heavy metal-contaminated soil in Europe, Ponte Nossa (Noss/IT), and from non-metalliferous (NM) soil. Noss exhibits enhanced hypertolerance and attenuated accumulation of cadmium (Cd), and transcriptomic Cd responsiveness is decreased, compared to plants of NM soil origin. Among the condition-independent transcriptome characteristics of Noss, the most highly overrepresented functional class of “meiotic cell cycle” comprises 21 transcripts with elevated abundance in vegetative tissues, in particular ARGONAUTE 9 (AGO9) and the synaptonemal complex transverse filament protein-encoding ZYP1a/b. Increased AGO9 transcript levels in Noss are accompanied by decreased long terminal repeat retrotransposon expression, and are shared by plants from milder metalliferous sites in Poland and Germany. Expression of IRON-REGULATED TRANSPORTER1 (IRT1) is very low and of HEAVY METAL ATPASE2 (HMA2) strongly elevated in Noss, which can account for its specific Cd handling. In plants adapted to the most extreme abiotic stress, broadly enhanced functions comprise genes with likely roles in somatic genome integrity maintenance, accompanied by few alterations in stress-specific functional networks.

Spatial genetic structure within a metallicolous population of Arabidopsis halleri, a clonal, self-incompatible and heavy-metal-tolerant species

2004 ◽  
Vol 13 (10) ◽  
pp. 2959-2967 ◽  
Author(s):  
FABIENNE VAN ROSSUM ◽  
ISABELLE BONNIN ◽  
STÉPHANE FÉNART ◽  
MAXIME PAUWELS ◽  
DANIEL PETIT ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Genetic Structure ◽  
Spatial Genetic Structure ◽  
Arabidopsis Halleri ◽  
Tolerant Species

scholarly journals The tumor suppressor microRNA let-7 inhibits human LINE-1 retrotransposition

2020 ◽  
Author(s):  
Pablo Tristán-Ramos ◽  
Alejandro Rubio-Roldan ◽  
Guillermo Peris ◽  
Laura Sánchez ◽  
Suyapa Amador-Cubero ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Human Lung ◽  
Structure And Function ◽  
Human Lung Cancer ◽  
Genome Integrity ◽  
Class 1 ◽  
Somatic Genome ◽  
Human Line ◽  
And Function ◽  
Long Interspersed Element

ABSTRACTNearly half of the human genome is made of transposable elements (TEs) whose activity continues to impact its structure and function. Among them, Long INterspersed Element class 1 (LINE-1 or L1) elements are the only autonomously active TEs in humans. L1s are expressed and mobilized in different cancers, generating mutagenic insertions that could affect malignancy. Tumor suppressor microRNAs are ∼22nt RNAs that post-transcriptionally regulate oncogene expression and are frequently downregulated in cancer. Here we explore whether they also influence L1 mobilization. We found that downregulation of let-7 correlates with accumulation of L1 insertions in human lung cancer. Furthermore, we demonstrate that let-7 binds to the L1 mRNA and impairs the translation of the second L1-encoded protein, ORF2p, reducing its mobilization. Overall, our data uncover a new role for let-7, one of the most relevant microRNAs, which is to maintain somatic genome integrity by restricting L1 retrotransposition.

scholarly journals Negative Regulators of an RNAi-Heterochromatin Positive Feedback Loop Safeguard Somatic Genome Integrity in Tetrahymena

Cell Reports ◽  
2017 ◽  
Vol 18 (10) ◽  
pp. 2494-2507 ◽  
Author(s):  
Jan H. Suhren ◽  
Tomoko Noto ◽  
Kensuke Kataoka ◽  
Shan Gao ◽  
Yifan Liu ◽  
...  
Keyword(s):  
Positive Feedback ◽  
Feedback Loop ◽  
Genome Integrity ◽  
Negative Regulators ◽  
Positive Feedback Loop ◽  
Somatic Genome

Heavy metal specificity of cellular tolerance in two hyperaccumulating plants, Arabidopsis halleri and Thlaspi caerulescens

2004 ◽  
Vol 164 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Laurence Marquès ◽  
Magalie Cossegal ◽  
Stéphanie Bodin ◽  
Pierre Czernic ◽  
Michel Lebrun
Keyword(s):  
Heavy Metal ◽  
Thlaspi Caerulescens ◽  
Arabidopsis Halleri ◽  
Metal Specificity ◽  
Cellular Tolerance ◽  
Hyperaccumulating Plants

scholarly journals Plants accumulating heavy metals in the Sudety Mts

2011 ◽  
Vol 75 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Teresa Brej ◽  
Jerzy Fabiszewski
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Vascular Plants ◽  
Dry Weight ◽  
Systematic Position ◽  
Plant Biodiversity ◽  
Arabidopsis Halleri ◽  
Ore Deposit ◽  
Nickel Zinc ◽  
Armeria Maritima

The Sudeten flora consists of some plants we can recognize as heavy metal accumulators. Between others there are: <em>Thlaspi caerulescens</em>, <em>Arabidopsis halleri</em>, <em>Armeria maritima</em> ssp. <em>halleri</em> s.l. and probably the endemic fern <em>Asplenium onopteris</em> var. <em>silesiaca</em>. The authors present the concentrations of some important heavy metals measured in aboveground plant dry weight. The highest concentration of zinc was 8220 ppm (<em>Thlaspi</em>), nickel - 3100 ppm (<em>Thlaspi</em>), lead - 83 ppm (<em>Armeria</em>), copper - 611 ppm (<em>Arabidopsis</em>) and cadmium - 28 ppm (<em>Thlaspi</em>). The concentrations depend rather on species or population specification than on ore deposit quality. There are no typical hyperaccumulator among plants we have examined, but some signs of hyperaccumulation of nickel, zinc and lead could be observed. There are no typical endemic taxa, only <em>Asplenium onopteris</em> var. <em>silesiaca</em> and <em>Armeria maritima</em> ssp. <em>halleri</em> may be recognized as neoendemic taxa, but still of unclear systematic position. During the study we tried to find out why some Sudeten vascular plants do not develop heavy metals hyperaccumulation and why they are rather latent hyperaccumulators. Finally, we suggest to protect some metallicolous areas in spite they are rather territories with low plant biodiversity.

scholarly journals Genomic analysis for heavy metal resistance in S. maltophilia

2018 ◽  
Author(s):  
Wenbang Yu ◽  
Xiaoxiao Chen ◽  
Yilin Sheng ◽  
Qinghong Hong
Keyword(s):  
Heavy Metal ◽  
Gc Content ◽  
Genomic Analysis ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Single Chromosome ◽  
Ion Transporter ◽  
Protein Encoding ◽  
Mercury Detoxification

AbstractStenotrophomonas maltophilia is highly resistant to heavy metals, but the genetic knowledge of metal resistance in S. maltophilia is poorly understood. In this study, the genome of S.maltophilia Pho isolated from the contaminated soil near a metalwork factory was sequenced using PacBio RS II. Its genome is composed of a single chromosome with a GC content of 66.4% and 4434 protein-encoding genes. Comparative analysis revealed high syntney between S.maltophilia Pho and the model strain, S.maltophilia K279a. Then, the type and number of mechanisms for heavy metal uptake were analyzed firstly. Results revealed 7 unspecific ion transporter genes and 13 specific ion transporter genes, most of which were involved in iron transport. But the sulfate permeases belonging to the family of SulT/CysP that can uptake chromate and the high affinity ZnuABC/SitABCD were absent. Secondly, the putative genes controlling metal efflux were identified. Results showed that this bacterium encoded 5 CDFs, 1 copper exporting ATPase and 4 RND systems, including 2 CzcABC efflux pumps. Moreover, the putative metal transformation genes including arsenate and mercury detoxification genes were also identified. This study may provide useful information on the metal resistance mechanisms of S.maltophilia.

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