scholarly journals Mass Spectrometry-Based Absolute Quantification of Single Xenopus Embryo Proteomes

2018 ◽  
Vol 2019 (6) ◽  
pp. pdb.prot098376 ◽  
Author(s):  
Rik G.H. Lindeboom ◽  
Arne H. Smits ◽  
Matteo Perino ◽  
Gert Jan C. Veenstra ◽  
Michiel Vermeulen
Keyword(s):  
Mass Spectrometry ◽  
Absolute Quantification ◽  
Xenopus Embryo

High‐throughput, in‐depth and estimated absolute quantification of plasma proteome using data‐independent acquisition/mass spectrometry (“HIAP‐DIA”)

PROTEOMICS ◽  
2021 ◽  
pp. 2000264
Author(s):  
Yue Zhou ◽  
Zengqi Tan ◽  
Peng Xue ◽  
Yi Wang ◽  
Xiang Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Absolute Quantification ◽  
Plasma Proteome ◽  
Data Independent Acquisition ◽  
Using Data

Absolute Quantification of E1, Ubiquitin-Like Proteins and Nedd8–MLN4924 Adduct by Mass Spectrometry

2013 ◽  
Vol 67 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Xiaofeng Yang ◽  
James E. Brownell ◽  
Qing Xu ◽  
Fengying Zhu ◽  
Jingya Ma ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Absolute Quantification

scholarly journals Single-Cell Mass Spectrometry for Discovery Proteomics: Quantifying Translational Cell Heterogeneity in the 16-Cell Frog (Xenopus) Embryo

2016 ◽  
Vol 55 (7) ◽  
pp. 2454-2458 ◽  
Author(s):  
Camille Lombard-Banek ◽  
Sally A. Moody ◽  
Peter Nemes
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Cell Mass ◽  
Xenopus Embryo ◽  
Cell Heterogeneity ◽  
Cell Mass Spectrometry

scholarly journals Multiple Heart-Cutting Two Dimensional Liquid Chromatography And Isotope Dilution Tandem Mass Spectrometry For The Absolute Quantification Of Proteins In Human Serum

2021 ◽  
pp. 339022
Author(s):  
Amanda Suárez Fernández ◽  
Pablo Rodríguez-González ◽  
Lydia Álvarez ◽  
Montserrat García ◽  
Héctor González Iglesias ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Human Serum ◽  
Isotope Dilution ◽  
Absolute Quantification ◽  
Tandem Mass ◽  
Two Dimensional ◽  
The Absolute

scholarly journals Ultraperformance Liquid Chromatography Isotope Dilution Tandem Mass Spectrometry for the Absolute Quantification of Proteins and Peptides

2008 ◽  
Vol 80 (19) ◽  
pp. 7649-7649
Author(s):  
Leah G. Luna ◽  
Tracie L. Williams ◽  
James L. Pirkle ◽  
John R. Barr
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Isotope Dilution ◽  
Absolute Quantification ◽  
Tandem Mass ◽  
The Absolute ◽  
Proteins And Peptides

scholarly journals Structural Characterization and Absolute Quantification of Microcystin Peptides Using Collision-Induced and Ultraviolet Photo-Dissociation Tandem Mass Spectrometry

2018 ◽  
Vol 29 (9) ◽  
pp. 1812-1825 ◽  
Author(s):  
Troy J. Attard ◽  
Melissa D. Carter ◽  
Mengxuan Fang ◽  
Rudolph C. Johnson ◽  
Gavin E. Reid
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Absolute Quantification ◽  
Tandem Mass

Absolute quantification of Bet v 1 in birch polymerized allergenic extracts via mass spectrometry targeted analysis

2021 ◽  
Author(s):  
Raquel Moya ◽  
M. Antonia Odena ◽  
Mayte Gallego ◽  
Eliandre de Oliveira ◽  
Jerónimo Carnés
Keyword(s):  
Mass Spectrometry ◽  
Absolute Quantification ◽  
Bet V 1 ◽  
Targeted Analysis ◽  
Allergenic Extracts

scholarly journals Biomarker Discovery for Cytochrome P450 1A2 Activity Assessment in Rats, Based on Metabolomics

Metabolites ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 77 ◽  
Author(s):  
Xiao Pu ◽  
Yiqiao Gao ◽  
Ruiting Li ◽  
Wei Li ◽  
Yuan Tian ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cytochrome P450 ◽  
Biomarker Discovery ◽  
Absolute Quantification ◽  
Pharmacokinetic Analysis ◽  
Large Individual ◽  
Mrna Levels ◽  
Cytochrome P450 1A2 ◽  
Interindividual Variations ◽  
Highly Correlated

Cytochrome P450 1A2 (CYP1A2) is one of the major CYP450 enzymes (CYPs) in the liver, and participates in the biotransformation of various xenobiotics and endogenous signaling molecules. The expression and activity of CYP1A2 show large individual differences, due to genetic and environmental factors. In order to discover non-invasive serum biomarkers associated with hepatic CYP1A2, mass spectrometry-based, untargeted metabolomics were first conducted, in order to dissect the metabolic differences in the serum and liver between control rats and β-naphthoflavone (an inducer of CYP1A2)-treated rats. Real-time reverse transcription polymerase chain reaction and pharmacokinetic analysis of phenacetin and paracetamol were performed, in order to determine the changes of mRNA levels and activity of CYP1A2 in these two groups, respectively. Branched-chain amino acids phenylalanine and tyrosine were ultimately focalized, as they were detected in both the serum and liver with the same trends. These findings were further confirmed by absolute quantification via a liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based targeted metabolomics approach. Furthermore, the ratio of phenylalanine to tyrosine concentration was also found to be highly correlated with CYP1A2 activity and gene expression. This study demonstrates that metabolomics can be a potentially useful tool for biomarker discovery associated with CYPs. Our findings contribute to explaining interindividual variations in CYP1A2-mediated drug metabolism.

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