Structure-Function Analysis of the Coiled-Coil and Leucine-Rich Repeat Domains of the RPS5 Disease Resistance Protein

Wheat blast is a devastating disease which is baffling scientists from its inception. This study characterized the blast resistance related protein domains with a view to develop molecular markers to identify resistant wheat genotypes against Blast fungus Magnaporthe oryzae. A genome browse analysis detected that the candidate resistance gene against blast could be located in several different chromosomes. An in silico analysis was collected with fifty nucleotide-binding site leucine-rich repeat (NBS-LRR), leucine-rich repeat (LRR), pathogenesis and resistance protein-encoding accessions on the basis of the previous resistance report. The phylogenetic tree of those putative resistance accessions, bearing resistance related protein-encoding domains, showed that an NBS-LRR accession JP957107.1 has 67% similarity with the disease resistance protein domain encoding accession of Brazilian resistant cultivar Thatcher. By contrast, the rice blast resistance Pita gene has 72% similarity with 18 pathogenesis protein domain encoding accessions. Among putative protein domains, disease resistance protein of Thatcher has 78% similarity with two NBS-LRR protein domains AAZ99757.1 and AAZ99757.1. Eighteen microsatellite markers were designed from eighteen putative NBS-LRR protein encoding accessions along with Piz3 marker. The 19 markers were unable to separate resistant and susceptible genotypes. Diffused versus conspicuous bands indicated either presence of insertion/deletion (InDel) or single nucleotide polymorphism (SNP) among wheat genotypes. Detection of InDel or SNP markers is a subject of further investigation. Additional markers are needed to be designed using new NBS-LRR, pathogenesis, coiled-coil (CC), translocated intimin receptor (TIR) resistance protein encoding accessions to find out markers specific for blast resistance. J. Bangladesh Agril. Univ. 17(2): 161–171, June 2019

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The Chloroplastic Protein THF1 Interacts with the Coiled-Coil Domain of the Disease Resistance Protein N′ and Regulates Light-Dependent Cell Death

PLANT PHYSIOLOGY ◽

10.1104/pp.16.00234 ◽

2016 ◽

Vol 171 (1) ◽

pp. 658-674 ◽

Cited By ~ 21

Author(s):

Louis-Philippe Hamel ◽

Ken-Taro Sekine ◽

Thérèse Wallon ◽

Yuji Sugiwaka ◽

Kappei Kobayashi ◽

...

Keyword(s):

Cell Death ◽

Disease Resistance ◽

Coiled Coil ◽

Coiled Coil Domain ◽

Disease Resistance Protein ◽

Dependent Cell ◽

Resistance Protein

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Activation of a plant nucleotide binding-leucine rich repeat disease resistance protein by a modified self protein

Cellular Microbiology ◽

10.1111/j.1462-5822.2012.01779.x ◽

2012 ◽

Vol 14 (7) ◽

pp. 1071-1084 ◽

Cited By ~ 39

Author(s):

Brody J. DeYoung ◽

Dong Qi ◽

Sang-Hee Kim ◽

Thomas P. Burke ◽

Roger W. Innes

Keyword(s):

Disease Resistance ◽

Nucleotide Binding ◽

Leucine Rich Repeat ◽

Disease Resistance Protein ◽

Resistance Protein

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Genome-Wide Identification of Powdery Mildew Resistance in Common Bean (Phaseolus vulgaris L.)

Frontiers in Genetics ◽

10.3389/fgene.2021.673069 ◽

2021 ◽

Vol 12 ◽

Author(s):

Papias H. Binagwa ◽

Sy M. Traore ◽

Marceline Egnin ◽

Gregory C. Bernard ◽

Inocent Ritte ◽

...

Keyword(s):

Disease Resistance ◽

Powdery Mildew ◽

Common Bean ◽

Resistance Genes ◽

Significant Snps ◽

Leucine Rich Repeat ◽

Genome Wide ◽

Disease Resistance Protein ◽

Resistance Protein ◽

The Common

Genome-wide association studies (GWAS) have been utilized to detect genetic variations related to several agronomic traits and disease resistance in common bean. However, its application in the powdery mildew (PM) disease to identify candidate genes and their location in the common bean genome has not been fully addressed. Single-nucleotide polymorphism (SNP) genotyping with a BeadChip containing 5398 SNPs was used to detect genetic variations related to PM disease resistance in a panel of 211 genotypes grown under two field conditions for two consecutive years. Significant SNPs identified on chromosomes Pv04 and Pv10 were repeatable, ensuring the phenotypic data’s reliability and the causal relationship. A cluster of resistance genes was revealed on the Pv04 of the common bean genome, coiled-coil-nucleotide-binding site–leucine-rich repeat (CC-NBS-LRR, CNL), and Toll/interleukin-1 receptor-nucleotide-binding site–leucine-rich repeat type (TIR-NBS-LRR, TNL)-like resistance genes were identified. Furthermore, two resistance genes, Phavu_010G1320001g and Phavu_010G136800g, were also identified on Pv10. Further sequence analysis showed that these genes were homologs to the disease-resistance protein (RLM1A-like) and the putative disease-resistance protein (At4g11170.1) in Arabidopsis. Significant SNPs related to two LRR receptor-like kinases (RLK) were only identified on Pv11 in 2018. Many genes encoding the auxin-responsive protein, TIFY10A protein, growth-regulating factor five-like, ubiquitin-like protein, and cell wall RBR3-like protein related to PM disease resistance were identified nearby significant SNPs. These results suggested that the resistance to PM pathogen involves a network of many genes constitutively co-expressed.

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