scholarly journals Molecular characterization of Arabidopsis GAL4/UAS enhancer trap lines identifies novel cell type-specific promoters

2016 ◽  
pp. pp.00213.2016 ◽  
Author(s):  
Tatyana Radoeva ◽  
Colette A. ten Hove ◽  
Shunsuke Saiga ◽  
Dolf Weijers
Keyword(s):  
Molecular Characterization ◽  
Enhancer Trap ◽  
Cell Type ◽  
Cell Type Specific ◽  
Enhancer Trap Lines

scholarly journals Connectivity characterization of the mouse basolateral amygdalar complex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Houri Hintiryan ◽  
Ian Bowman ◽  
David L. Johnson ◽  
Laura Korobkova ◽  
Muye Zhu ◽  
...  
Keyword(s):  
Granular Cell ◽  
Cell Types ◽  
Projection Neurons ◽  
Cell Type ◽  
Connectivity Map ◽  
Analysis Techniques ◽  
Domain Specific ◽  
Cell Type Specific ◽  
Unique Domain

AbstractThe basolateral amygdalar complex (BLA) is implicated in behaviors ranging from fear acquisition to addiction. Optogenetic methods have enabled the association of circuit-specific functions to uniquely connected BLA cell types. Thus, a systematic and detailed connectivity profile of BLA projection neurons to inform granular, cell type-specific interrogations is warranted. Here, we apply machine-learning based computational and informatics analysis techniques to the results of circuit-tracing experiments to create a foundational, comprehensive BLA connectivity map. The analyses identify three distinct domains within the anterior BLA (BLAa) that house target-specific projection neurons with distinguishable morphological features. We identify brain-wide targets of projection neurons in the three BLAa domains, as well as in the posterior BLA, ventral BLA, posterior basomedial, and lateral amygdalar nuclei. Inputs to each nucleus also are identified via retrograde tracing. The data suggests that connectionally unique, domain-specific BLAa neurons are associated with distinct behavior networks.

Cytometric Profiling of CD133+ Cells in Human Colon Carcinoma Cell Lines Identifies a Common core Phenotype and Cell Type-specific Mosaics

2013 ◽  
Vol 28 (3) ◽  
pp. 267-273 ◽  
Author(s):  
Marica Gemei ◽  
Rosa Di Noto ◽  
Peppino Mirabelli ◽  
Luigi Del Vecchio
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Cell Type ◽  
Carcinoma Cell Lines ◽  
Cell Type Specific

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

Characterization of cell type-specific S100B expression in the mouse olfactory bulb

Cell Calcium ◽  
2021 ◽  
Vol 94 ◽  
pp. 102334
Author(s):  
Xin Su ◽  
Tamara Vasilkovska ◽  
Nicole Fröhlich ◽  
Olga Garaschuk
Keyword(s):  
Olfactory Bulb ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals Identification and characterization of cell type-specific and ubiquitous chromatin regulatory structures in the human genome

PLoS Genetics ◽  
2005 ◽  
Vol preprint (2007) ◽  
pp. e136
Author(s):  
Hualin Xi ◽  
Hennady P Shulha ◽  
Jane M Lin ◽  
Teresa R Vales ◽  
Yutao Fu ◽  
...  
Keyword(s):  
Human Genome ◽  
Cell Type ◽  
Cell Type Specific ◽  
Identification And Characterization

scholarly journals Characterization of Cre recombinase  mouse lines enabling cell type‐specific targeting of postnatal intervertebral discs

2019 ◽  
Vol 234 (9) ◽  
pp. 14422-14431 ◽  
Author(s):  
Yixin Zheng ◽  
Xuejie Fu ◽  
Qingbai Liu ◽  
Shengqi Guan ◽  
Cunchang Liu ◽  
...  
Keyword(s):  
Cre Recombinase ◽  
Intervertebral Discs ◽  
Cell Type ◽  
Specific Targeting ◽  
Cell Type Specific ◽  
Mouse Lines

scholarly journals Multilayer OMICS characterization of APOE‐modulated isogenic hiPSCs elucidates cell‐type–specific mechanisms modified by APOE: A study of the IMI ADAPTED Consortium

2020 ◽  
Vol 16 (S2) ◽  
Author(s):  
Janina S. Ried ◽  
Marco Rocha Curado ◽  
María Eugenia Sáez ◽  
Lamiaa Bahnassawy ◽  
Heyne Lee ◽  
...  
Keyword(s):  
Cell Type ◽  
Cell Type Specific

scholarly journals Renal Tumors of Childhood—A Histopathologic Pattern-Based Diagnostic Approach

Cancers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 729 ◽  
Author(s):  
Ariadne H.A.G. Ooms ◽  
Gordan M. Vujanić ◽  
Ellen D’Hooghe ◽  
Paola Collini ◽  
Aurore L’Herminé-Coulomb ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Pediatric Oncology ◽  
Correct Diagnosis ◽  
Renal Tumors ◽  
Pediatric Tumors ◽  
Cell Type ◽  
Appropriate Treatment ◽  
Histologic Pattern

Renal tumors comprise approximately 7% of all malignant pediatric tumors. This is a highly heterogeneous group of tumors, each with its own therapeutic management, outcome, and association with germline predispositions. Histopathology is the key in establishing the correct diagnosis, and therefore pathologists with expertise in pediatric oncology are needed for dealing with these rare tumors. While each tumor shows different histologic features, they do have considerable overlap in cell type and histologic pattern, making the diagnosis difficult to establish, if based on routine histology alone. To this end, ancillary techniques, such as immunohistochemistry and molecular analysis, can be of great importance for the correct diagnosis, resulting in appropriate treatment. To use ancillary techniques cost-effectively, we propose a pattern-based approach and provide recommendations to aid in deciding which panel of antibodies, supplemented by molecular characterization of a subset of genes, are required.

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