scholarly journals Renal Tumors of Childhood—A Histopathologic Pattern-Based Diagnostic Approach

Cancers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 729 ◽  
Author(s):  
Ariadne H.A.G. Ooms ◽  
Gordan M. Vujanić ◽  
Ellen D’Hooghe ◽  
Paola Collini ◽  
Aurore L’Herminé-Coulomb ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Pediatric Oncology ◽  
Correct Diagnosis ◽  
Renal Tumors ◽  
Pediatric Tumors ◽  
Cell Type ◽  
Appropriate Treatment ◽  
Histologic Pattern

Renal tumors comprise approximately 7% of all malignant pediatric tumors. This is a highly heterogeneous group of tumors, each with its own therapeutic management, outcome, and association with germline predispositions. Histopathology is the key in establishing the correct diagnosis, and therefore pathologists with expertise in pediatric oncology are needed for dealing with these rare tumors. While each tumor shows different histologic features, they do have considerable overlap in cell type and histologic pattern, making the diagnosis difficult to establish, if based on routine histology alone. To this end, ancillary techniques, such as immunohistochemistry and molecular analysis, can be of great importance for the correct diagnosis, resulting in appropriate treatment. To use ancillary techniques cost-effectively, we propose a pattern-based approach and provide recommendations to aid in deciding which panel of antibodies, supplemented by molecular characterization of a subset of genes, are required.

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

scholarly journals Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats

2015 ◽  
Vol 35 (12) ◽  
pp. 980-988 ◽  
Author(s):  
Caroline Spitz dos Santos ◽  
Vera Lúcia Teixeira de Jesus ◽  
Douglas McIntosh ◽  
Bruno Pereira Berto ◽  
Carlos Wilson Gomes Lopes
Keyword(s):  
Molecular Analysis ◽  
Morphological Analysis ◽  
Correct Diagnosis ◽  
Diagnostic Techniques ◽  
Tritrichomonas Foetus ◽  
Specific Primers ◽  
Transmission Electron ◽  
Appropriate Treatment ◽  
Simultaneous Infection ◽  
Pentatrichomonas Hominis

Abstract: Tritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle ending abruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian.

scholarly journals Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Eduardo Reséndiz ◽  
Helena Fernández-Sanz ◽  
José Francisco Domínguez-Contreras ◽  
Amelly Hyldaí Ramos-Díaz ◽  
Agnese Mancini ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Baja California ◽  
Clinical Signs ◽  
Chelonia Mydas ◽  
Baja California Sur ◽  
Epidermal Hyperplasia ◽  
Routine Monitoring ◽  
Hematological Values

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

scholarly journals Contemporary Renal Tumor Categorization With Biomarker and Translational Updates: A Practical Review

2019 ◽  
Vol 143 (12) ◽  
pp. 1477-1491 ◽  
Author(s):  
Alexander S. Taylor ◽  
Daniel E. Spratt ◽  
Saravana M. Dhanasekaran ◽  
Rohit Mehra
Keyword(s):  
World Health Organization ◽  
Molecular Characterization ◽  
Renal Tumor ◽  
Tumor Classification ◽  
World Health ◽  
Renal Tumors ◽  
World Health Organization Classification ◽  
The World ◽  
Health Organization

Context.— Renal tumor classification has evolved in recent decades, as evidenced by the comparable complexity of the 2016 revision to the World Health Organization Classification of Tumours of the Urinary System and Male Genital Organs. A recent expansion of the knowledge base surrounding the cells of origin and evolutionary genomic characteristics of renal tumors has led to molecular characterization of novel entities and enriched understanding of established entities. This pace of research and its implementation into clinical practice has again begun to surpass that of our own classification schemata, with significant discoveries having been made since the introduction of the 2016 revision to the World Health Organization classification. In particular, biomarkers for renal tumor diagnosis and prognosis are in translation for future clinical application. Objectives.— To provide a brief framework for clinical characterization of renal tumors rooted in morphologic assessment, to briefly review the current and future status of renal tumor biomarkers with an emphasis on practical use of these ancillary tools for accurate diagnosis, and to discuss the impact of emerging technologies and clinical trials relevant to renal cell carcinoma classification and biomarker development. Data Sources.— We review recent literature relevant to renal tumor classification (including established and proposed entities), focusing on molecular characterization and biomarker assessment. Conclusions.— Accurate renal tumor diagnosis requires an up-to-date understanding of renal tumor classification, including an awareness of morphologic clues that should stimulate consideration of molecularly defined entities, as well as the ancillary biomarker testing required to confirm diagnoses.

scholarly journals Molecular Characterization of Preneoplastic Lesions Provides Insight on the Development of Renal Tumors

2009 ◽  
Vol 175 (4) ◽  
pp. 1686-1698 ◽  
Author(s):  
Kerstin Stemmer ◽  
Heidrun Ellinger-Ziegelbauer ◽  
Hans-Jürgen Ahr ◽  
Daniel R. Dietrich
Keyword(s):  
Molecular Characterization ◽  
Renal Tumors ◽  
Preneoplastic Lesions

scholarly journals Circulating tumor DNA analysis enables molecular characterization of pediatric renal tumors at diagnosis

2018 ◽  
Vol 144 (1) ◽  
pp. 68-79 ◽  
Author(s):  
Irene Jiménez ◽  
Mathieu Chicard ◽  
Léo Colmet-Daage ◽  
Nathalie Clément ◽  
Adrien Danzon ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Dna Analysis ◽  
Circulating Tumor Dna ◽  
Renal Tumors ◽  
Tumor Dna

scholarly journals The fallacy of identification by neutralization in the light of cytopathology nonproducing enterovirus strains

2017 ◽  
Author(s):  
Moses Olubusuyi Adewumi ◽  
Temitope Oluwasegun Cephas Faleye ◽  
Johnson Adekunle Adeniji
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Specific Antisera ◽  
Southwest Nigeria ◽  
Coxsackievirus A24 ◽  
Structural Region

ABSTRACTWe describe the characterization of an enterovirus isolate recovered from untreated raw sewage in Ibadan, southwest Nigeria in 2010. The isolate was neutralized by specific antisera and consequently identified as Echovirus 7 (E7). Subsequent molecular characterization showed the isolate to be a mixture of E7 and Coxsackievirus A24 (CV-A24) thereby suggesting the CV-A24 strain to be non-cytopathology producing. Further molecular analysis suggested that the CV-A24 might have recombined with a Sabin poliovirus 2 (PV2) in its non-structural region. This is the first description of a Nigerian CV-A24 strain.

scholarly journals Integrated Strategy for Fast and Automated Molecular Characterization of Genes Involved in Craniosynostosis

2007 ◽  
Vol 53 (10) ◽  
pp. 1767-1774 ◽  
Author(s):  
Stefania Stenirri ◽  
Gabriella Restagno ◽  
Giovanni Battista Ferrero ◽  
Georgia Alaimo ◽  
Luca Sbaiz ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Correct Diagnosis ◽  
Apert Syndrome ◽  
Developmental Defect ◽  
Crouzon Syndrome ◽  
Coding Region ◽  
Fibroblast Growth Factor Receptors ◽  
Probe Hybridization ◽  
Dhplc Analysis

Abstract Background: Craniosynostosis, the premature fusion of 1 or more sutures of the skull, is a common congenital defect, with a prevalence of 1 in 2500 live births. Untreated progressive craniosynostosis leads to inhibition of brain growth and increased intracranial and intraorbital pressure. The heterogeneity of clinical phenotypes and the overlap of the various associated syndromes render the correct diagnosis of the different craniosynostoses particularly difficult. Methods: To identify 10 common mutations in the genes for fibroblast growth factor receptors 2 and 3 (FGFR2 and FGFR3), we developed a microelectronic microchip assay that exploited the PCR multiplexing format and coupled it with serial addressing and probe hybridization on the same pad. For the molecular characterization of patients who tested negative in the microchip screening, we also developed conditions for denaturing HPLC (DHPLC) analysis of the most mutated regions of FGFR2 and FGFR3 and the entire coding region of the TWIST1 gene. Results: In our cohort of 159 patients with various craniosynostosis syndromes, mutations were found in 100% of patients with Apert syndrome, 83.3% with Pfeiffer syndrome, 72.7% with Crouzon syndrome, 50.0% with Saethre-Chotzen syndrome, 27.7% with plagiocephaly, 31.8% with brachicephaly, 20% of complex cases, and 6.9% of mixed cases. No mutations were found in syndromic cases. Conclusions: The combined microchip-DHPLC strategy allows rapid and specific molecular diagnosis of craniosynostosis and is an effective tool for the medical and surgical management of these common congenital anomalies in a newborn or an infant with a developmental defect of the cranial vault.

scholarly journals Molecular Characterization of Moroccan Fig Germplasm Using Intersimple Sequence Repeat and Simple Sequence Repeat Markers To Establish A Reference Collection

HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 29-32 ◽  
Author(s):  
B. Khadari ◽  
A. Oukabli ◽  
M. Ater ◽  
A. Mamouni ◽  
J.P. Roger ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Simple Sequence Repeat ◽  
Germplasm Collection ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Reference Collection ◽  
Simple Sequence

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.

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